http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Jung-Pil Suh,Tae-Hwan Noh,Ki-Young Kim,Jeong-Ju Kim,Yeon-Gyu Kim,Kshirod K. Jena 한국작물학회 2009 Journal of crop science and biotechnology Vol.12 No.3
Bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo) is a destructive disease of rice in the major rice growing countries of Asia. In 2003, a serious bacterial blight epidemic occurred in the southwestern coastal areas in Korea, causing significant yield loss due to the emergence of a new race, K3a. IR24 near-isogenic lines containing Xa4, xa5, Xa7 and Xa21 genes conferred different degrees of resistance to the most virulent K3a isolate, HB01009 in an inoculation experiment in the greenhouse. Expression levels of the resistance genes, Xa4, xa5 and Xa21 were studied in two F2 populations derived from the crosses between elite japonica cultivars and an advanced backcross breeding line possessing Xa4, xa5 and Xa21 genes. F2 progenies segregated for K3a resistance (R) and susceptible (S) phenotypes in a ratio of 3(R):1(S) indicated that K3a resistance was controlled by a major dominant gene. Three PCR markers tightly linked to the resistance genes Xa4, xa5 and Xa21 confirmed the presence of the genes and their interaction with each gene. This study demonstrated that the Xa21 gene dominantly contributed to K3a resistance. However, the Xa4 gene also contributed to the full expression of resistance. The level of expression of strong resistance to K3a race was attributed to the presence of Xa21 and Xa4 genes irrespective of the presence of xa5 gene. Our results suggest that the R-gene combinations of Xa4+Xa21 could be a useful and effective strategy toward improving resistance to K3a race of Korean japonica cultivars. Bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo) is a destructive disease of rice in the major rice growing countries of Asia. In 2003, a serious bacterial blight epidemic occurred in the southwestern coastal areas in Korea, causing significant yield loss due to the emergence of a new race, K3a. IR24 near-isogenic lines containing Xa4, xa5, Xa7 and Xa21 genes conferred different degrees of resistance to the most virulent K3a isolate, HB01009 in an inoculation experiment in the greenhouse. Expression levels of the resistance genes, Xa4, xa5 and Xa21 were studied in two F2 populations derived from the crosses between elite japonica cultivars and an advanced backcross breeding line possessing Xa4, xa5 and Xa21 genes. F2 progenies segregated for K3a resistance (R) and susceptible (S) phenotypes in a ratio of 3(R):1(S) indicated that K3a resistance was controlled by a major dominant gene. Three PCR markers tightly linked to the resistance genes Xa4, xa5 and Xa21 confirmed the presence of the genes and their interaction with each gene. This study demonstrated that the Xa21 gene dominantly contributed to K3a resistance. However, the Xa4 gene also contributed to the full expression of resistance. The level of expression of strong resistance to K3a race was attributed to the presence of Xa21 and Xa4 genes irrespective of the presence of xa5 gene. Our results suggest that the R-gene combinations of Xa4+Xa21 could be a useful and effective strategy toward improving resistance to K3a race of Korean japonica cultivars.
Jung-Pil Suh,Jae-Hwan Roh,Young-Chan Cho,Seong-Sook Han,Yong-Hee Jeon,Kyung-Ho Kang,Yeon-Gyu Kim 한국육종학회 2008 한국육종학회지 Vol.40 No.3
Fifty-two Korean japonica rice cultivars were analyzed for leaf blast resistance and genotyped with 4 STS and 26 SSR markers flanking the specific chromosome sites linked with blast resistance genes. In our analysis of resistance genes in 52 japonica cultivars using STS markers tightly linked to Pib, Pita, Pi5(t) and Pi9(t), the blast nursery reaction of the cultivars possessing the each four major genes were not identical to that of the differential lines. Eight of the 26 SSR markers were associated with resistant phenotypes against the isolates of blast nursery as well as the specific Korean blast isolates, 90-008 (KI-1113), 03-177 (KJ-105). These markers were linked to Pit, Pish, Pib, Pi5(t), Piz, Pia, Pik, Pi18, Pita and Pi25(t) resistance gene loci. Three of the eight SSR markers, MRG5836, RM224 and RM7102 only showed significantly associated with the phenotypes of blast nursery test for two consecutive years. These three SSR markers also could distinguish between resistant and susceptible japonica cultivars. These results demonstrate the usefulness of marker-assisted selection and genotypic monitoring for blast resistance of rice in blast breeding programs.
Jung-Pil Suh,Sang-Nag Ahn,Young-Chan Cho,Kyong-Ho Kang,Im-Soo Choi,Yeon-Gyu Kim,Hak-Soo Suh,Hung-Goo Hwang 한국육종학회 2005 한국육종학회지 Vol.37 No.5
An interspecific BC3F2 population (Milyang 23/O. glaberrima/3*Milyang 23) consisting of 141 lines was evaluated for8 traits associated with yield. Oryza glaberrima (IRGC #103544) was phenotypically inferior to all of the traits examined in thisicle and grain yield. A genetic linkage map generated for this population consisted of 124 SSR markers with an average intervallength of 13.1 cM. The percentage of O. glaberrima genome, which was calculated on the number of the exotic gene introgres-sions contained in the 141 BC3F2 lines, ranged from 3.9 to 25.4% with a mean of 12.8%. Of the 37 QTLs associated with yield andyield components, 24 alleles from O. glaberrima were favorable. However, eight QTLs were significantly associated with yieldand five alleles of O. glaberrimafrom 1.5 to 2.2 tons/ha. Exploitation of the genetic potential of exotic species using molecular markers might provide a new oppor-tunity to improve yield and quantitative traits in rice breeding program.
Suh, Jung-Pil,Jeung, Ji-Ung,Noh, Tae-Hwan,Cho, Young-Chan,Park, So-Hyun,Park, Hyun-Su,Shin, Mun-Sik,Kim, Chung-Kon,Jena, Kshirod K Springer New York 2013 Rice Vol.6 No.-
<P><B>Background</B></P><P>The development of resistant cultivars has been the most effective and economical strategy to control bacterial leaf blight (BB) disease of rice caused by <I>Xanthomonas oryzae</I> pv. <I>oryzae</I> (<I>Xoo</I>). Molecular markers have made it possible to identify and pyramid valuable genes of agronomic importance in resistance rice breeding. In this study, three resistance genes (<I>Xa4</I> + <I>xa5</I> + <I>Xa21</I>) were transferred from an indica donor (IRBB57), using a marker-assisted backcrossing (MAB) breeding strategy, into a BB-susceptible elite japonica rice cultivar, Mangeumbyeo, which is high yielding with good grain quality.</P><P><B>Results</B></P><P>Our analysis led to the development of three elite advanced backcross breeding lines (ABL) with three resistance genes by foreground and phenotypic selection in a japonica genetic background without linkage drag. The background genome recovery of the ABL expressed more than 92.1% using genome-wide SSR marker analysis. The pathogenicity assays of three resistance-gene-derived ABL were conducted under glasshouse conditions with the 18 isolates of <I>Xoo</I> prevalent in Korea. The ABL exhibited very small lesion lengths, indicating a hypersensitive reaction to all 18 isolates of <I>Xoo</I>, with agronomic and grain quality traits similar to those of the recurrent parent. Pyramiding the resistance genes <I>Xa4, xa5</I> and <I>Xa21</I> provided a higher resistance to <I>Xoo</I> than the introduction of the individual resistance genes. Additionally, the combination of two dominant and one recessive BB resistance gene did not express any negative effect on agronomic traits in the ABL.</P><P><B>Conclusions</B></P><P>The strategy of simultaneous foreground and phenotypic selection to introduce multiple R genes is very useful to reduce the cost and the time required for the isolation of desirable recombinants with target resistance genes in rice. The resistance-gene-derived ABL have practical breeding value without a yield penalty by providing broad-spectrum resistance against most of the existing isolates of BB in South Korea and will have a high impact on the yield stability and sustainability of rice productivity.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/1939-8433-6-5) contains supplementary material, which is available to authorized users.</P>
Field Performance and SSR Analysis of Drought QTL Introgression Lines of Rice
( Jung Pil Suh ),( Yong Jae Won ),( Eok Keun Ahn ),( Jeong Heui Lee ),( Woon Goo Ha ),( Myeong Ki Kim ),( Young Chan Cho ),( Eung Gi Jeong ),( Bo Kyeong Kim ) 한국육종학회 2014 Plant Breeding and Biotechnology Vol.2 No.2
A set of five Korean rice cultivars and seven drought-tolerant indica lines were screened under irrigated non-stress and drought-stressed conditions in the 2011 and 2012 dry seasons at IRRI, Philippines. The drought-stressed experiment received mild to moderate stress. Under drought stress, ‘IR86918-B-439-B’ had the highest grain yield among all tested lines and ‘Hanarembyeo’ had the highest grain yield among the five Korean rice cultivars. ‘IR86918-B-439-B’ also had the highest yield under irrigated non-stress conditions. The grain yield of ‘Hanareumbyeo’ was similar to ‘IR86918-B-439-B’ under non-stress conditions. SSR marker analysis was performed using 125 SSR markers for detection of polymorphic markers between the Korean rice cultivars and the drought-tolerant indica lines, and for genetic diversity analysis. Twelve polymorphic markers were identified in the region of three major drought QTLs (DTY1.1, DTY2.2, DTY3.1) in two of the Korean rice cultivars and three of the drought-tolerant lines. These polymorphic markers will be useful as foreground genotyping markers for drought-QTL introgression in Korean rice genetic backgrounds.
Jung Pil Suh,Young Chan Cho,Soo Jin Kwon,Im Soo Choi,Ha Chul Hong,Yeon Gyu Kim,Sang Nag Ahn,Hung Goo Hwang 한국육종학회 2006 한국육종학회지 Vol.38 No.2
A recombinant inbred lines (RILs) consisting of 231 lines, derived from a japonica (Suweon365) and a japonica (Chu-cheongbyeo) rice, was used to investigate the genetic factors affecting cooking and eating quality of rice. Alkali digestion valueloci (QTLs
( Jung Pil Suh ),( Young Chan Cho ),( Yong Jae Won ),( Eok Keun Ahn ),( Man Kee Baek ),( Myeong Ki Kim ),( Bo Kyeong Kim ),( Kshirod K. Jena ) 한국육종학회 2015 Plant Breeding and Biotechnology Vol.3 No.4
Advances in plant molecular techniques have dramatically widened the applicability of gene identification and pyramiding valuable genes. This study was carried out to pyramid five resistance genes for biotic stress into the japonica rice cultivar using marker-assisted selection (MAS) and marker-assisted background analysis of selected progenies using SSR markers. The Pi40, Xa4, xa5, Xa21 and Bph18 genes were combined in Jinbubyeo, a Korean japonica rice variety using MAS. Gene specific co-dominant PCR-based markers were used to select for homozygous recombinant lines in a segregating population derived from a cross between the parental homozygous resistant gene introgression lines. We had successfully developed multiple gene pyramided breeding lines (GPLs) for bacterial blight, blast, and brown planthopper using MAS in rice. The GPLs exhibited high resistance against biotic stress and had around 93% of the genetic background of the recurrent parent Jinbubyeo based on SSR graphical mapping. The yield and agronomic traits of the GPLs were similar to those of the recurrent parent, indicating that there is no apparent agronomic trait penalty associated with the presence of the resistance genes. The strategy of simultaneous foreground and phenotypic selection to introduce multiple R genes is very useful to reduce the cost and the time required for the isolation of desirable recombinants with target resistance genes in rice. The GPLs could be useful to enhance effective resistance for biotic stress and produce stable grain yield in japonica rice breeding programs.