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Xia, Xiao-Xia,Han, Mee-Jung,Lee, Sang Yup,Yoo, Jong-Shin WILEY-VCH Verlag 2008 Proteomics Vol.8 No.10
<P>Escherichia coli BL21 (DE3) and W3110 strains, belonging to the family B and K-12, respectively, have been most widely employed for recombinant protein production. During the excretory production of recombinant proteins by high cell density cultivation (HCDC) of these strains, other native E. coli proteins were also released. Thus, we analyzed the extracellular proteomes of E. coli BL21 (DE3) and W3110 during HCDC. E. coli BL21 (DE3) released more than twice the amount of protein compared with W3110 during HCDC. A total of 204 protein spots including 83 nonredundant proteins were unambiguously identified by 2-DE and MS. Of these, 32 proteins were conserved in the two strains, while 20 and 33 strain-specific proteins were identified for E. coli BL21 (DE3) and W3110, respectively. More than 70% of identified proteins were found to be of periplasmic origin. The outer membrane proteins, OmpA and OmpF, were most abundant. Two strains showed much different patterns in their released proteins. Also, cell density-dependent variations in the released proteins were observed in both strains. These findings summarized as reference proteome maps will be useful for studying protein release in further detail, and provide new strategies for enhanced excretory production of recombinant proteins.</P>
Xia, Yun-Xia,Ni, Yi-Qing,Zhang, Chi Techno-Press 2014 Structural monitoring and maintenance Vol.1 No.4
Long-span cable-supported bridges are flexible structures vulnerable to unsymmetric loadings such as railway traffic and strong wind. The torsional dynamic response of long-span cable-supported bridges under running trains and/or strong winds may deform the railway track laid on the bridge deck and affect the running safety of trains and the comfort of passengers, and even lead the bridge to collapse. Therefore, it is eager to figure out the torsional dynamic response of long-span cable-supported bridges under running trains and/or strong winds. The Tsing Ma Bridge (TMB) in Hong Kong is a suspension bridge with a main span of 1,377 m, and is currently the world's longest suspension bridge carrying both road and rail traffic. Moreover, this bridge is located in one of the most active typhoon-prone regions in the world. A wind and structural health monitoring system (WASHMS) was installed on the TMB in 1997, and after 17 years of successful operation it is still working well as desired. Making use of one-year monitoring data acquired by the WASHMS, the torsional dynamic responses of the bridge deck under rail traffic and strong winds are analyzed. The monitoring results demonstrate that the differences of vertical displacement at the opposite edges and the corresponding rotations of the bridge deck are less than 60 mm and $0.1^{\circ}$ respectively under weak winds, and less than 300 mm and $0.6^{\circ}$ respectively under typhoons, implying that the torsional dynamic response of the bridge deck under rail traffic and wind loading is not significant due to the rational design.
SUN XIA(Sun, Xia),박상현(Park, Sanghyun),권영구(Kwon, Younggoo) 한국IT서비스학회 2009 한국IT서비스학회 학술대회 논문집 Vol.2009 No.1
최근 차량용 IT 기술이 발달함에 따라 네비게이션, 위치추적, 인터넷 접수, 원격 차량진단, 사고감지, 긴급구난, 교통정보 등을 제공하는 서비스들이 등장하고 있다. 또한 차량의 편의성과 안정성을 추구함과 동시에 친환경 등에 대한 요구도 증가하고 있다. 그리고 최근차량 상호간 정보의 교환이 더욱 필요해짐에 따라 차량간의 무선 통신 기능이 중요해지고 있으며 차량 내의 네트워크 기술에 대한 연구도 필요하다. 현재 차량 내 네트워크로는 CAN, LIN, MOST등의 유선으로 된 버스 시스템을 중심으로 한 차량 제어 시스템과 멀티미디어 시스템으로 크게 구분할 수 있다. 그러나 자동차 내에 장치 배선이 복잡해짐에 따라 차량의 무게 증가, 고장율의 증가, 연비 저하 등으로 이어지고 있다. 이러한 문제를 보완하기 위해 차량 내에 무선 센서 네트워크 시스템과의 통합 개발이 요구되고 있다. 본 논문에서는 빠르게 발전하고 있는 차량 내 네트워크에 대한 기술개발 동향을 분석하고, 유무선 통합형 차량 내 네트워크 응용 서비스들을 제시하고자 한다.
Dynamic analysis of high-speed railway train-bridge system after barge collision
Xia, Chaoyi,Ma, Qin,Song, Fudong,Wu, Xuan,Xia, He Techno-Press 2018 Structural Engineering and Mechanics, An Int'l Jou Vol.67 No.1
In this paper, a framework is proposed for dynamic analysis of train-bridge systems with a damaged pier after barge collision. In simulating the barge-pier collision, the concrete pier is considered to be nonlinear-inelastic, and the barge-bow is modeled as elastic-plastic. The changes of dynamic properties and deformation of the damaged pier, and the additional unevenness of the track induced by the change of deck profile, are analyzed. The dynamic analysis model for train-bridge coupling system with a damaged pier is established. Based on the framework, an illustrative case study is carried out with a $5{\times}32m$ simply-supported PC box-girder bridge and the ICE3 high-speed train, to investigate the dynamic response of the bridge with a damaged pier after barge collision and its influence on the running safety of high-speed train. The results show that after collision by the barge, the vibration properties of the pier and the deck profile of bridge are changed, forming an additional unevenness of the track, by which the dynamic responses of the bridge and the car-body accelerations of the train are increased, and the running safety of high-speed train is affected.
On the role of surface diffusion in determining the shape or morphology of noble-metal nanocrystals
Xia, Xiaohu,Xie, Shuifen,Liu, Maochang,Peng, Hsin-Chieh,Lu, Ning,Wang, Jinguo,Kim, Moon J.,Xia, Younan National Academy of Sciences 2013 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.110 No.17
<P>Controlling the shape or morphology of metal nanocrystals is central to the realization of their many applications in catalysis, plasmonics, and electronics. In one of the approaches, the metal nanocrystals are grown from seeds of certain crystallinity through the addition of atomic species. In this case, manipulating the rates at which the atomic species are added onto different crystallographic planes of a seed has been actively explored to control the growth pattern of a seed and thereby the shape or morphology taken by the final product. Upon deposition, however, the adsorbed atoms (adatoms) may not stay at the same sites where the depositions occur. Instead, they can migrate to other sites on the seed owing to the involvement of surface diffusion, and this could lead to unexpected deviations from a desired growth pathway. Herein, we demonstrated that the growth pathway of a seed is indeed determined by the ratio between the rates for atom deposition and surface diffusion. Our result suggests that surface diffusion needs to be taken into account when controlling the shape or morphology of metal nanocrystals.</P>
Dynamic analysis of coupled train - ladder track - elevated bridge system
Xia, He,Deng, Yushu,Xia, Chaoyi,De Roeck, G.,Qi, Lin,Sun, Lu Techno-Press 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.47 No.5
As a new type of vibration reduction, the ladder track system has been successfully used in engineering. In this paper, a numerical model of the train-track-viaduct system is established to study the dynamic responses of an elevated bridge with ladder track. The system is composed of a vehicle submodel, a track submodel and a bridge submodel, with the measured track irregularities as the system self-excitation. The whole time histories of a train running through an elevated bridge with $3{\times}27m$ continuous PC box girders are simulated. The dynamic responses of the bridge such as deflections, lateral and vertical accelerations, and the vehicle responses such as derailment factors, offload factors and car-body accelerations are calculated. The calculated results are partly validated through the comparison with the experimental data. Compared to the common slab track, adapting the ladder sleeper can effectively reduce the accelerations of the bridge girder, and also reduce the car-body accelerations and offload factors of the train vehicle.
Anti-Rollover Control Based on Stable Zone Partition of Counterbalanced Forklift Trucks
Xia Guang,Xia Yan,Tang Xiwen,Zhao Linfeng,Hu Jinfang 한국자동차공학회 2021 International journal of automotive technology Vol.22 No.6
To improve the anti-rollover capacity of a counterbalanced forklift, a two-stage rollover dynamic model is established on the basis of the forklift structure. Stable zones are divided according to the two-stage lateral load transfer rate: stable region, relatively stable region, dangerous zone and abnormal dangerous zone. An anti-rollover layered control strategy based on stable zone partition is proposed, and different anti-rollover control actuators are selected: dynamic balance weight, anti-rollover cylinder and steering cylinder. Anti-rollover controllers consist of the upper stable region identification controller, the middle-level controller based on model predictive control (MPC) and the lower layer executive controller. The upper stable region identification controller performs stable zone recognition based on the two-stage lateral load transfer rate. The middlelevel MPC controller calculates the required control torque with the body’s lateral angle and yaw rate as the control objectives. The lower layer executive controller controls the balance weight, anti-rollover cylinder and the steering cylinder according to the improved chain incremental allocation method to meet the target control torque. Simulation and real vehicle tests based on MATLAB/Simulink show that the anti-rollover control based on stable zone partition can greatly reduce the risk of forklift rollover and improve the forklift safety.