HCV-Specific Interleukin-21+CD4+ T Cells Responses Associated with Viral Control through the Modulation of HCV-Specific CD8+ T Cells Function in Chronic Hepatitis C Patients

Guo-Hua Feng,Fu-Sheng Wang,Ji-Yuan Zhang,Qing-Lei Zeng,Lei Jin,Junliang Fu,Bin Yang,Ying Sun,Tianjun Jiang,Xiangsheng Xu,Zheng Zhang,Jinhong Yuan,Liyuan Wu 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.4

Interleukin-21 (IL-21)+CD4+ T cells are involved in the immune response against hepatitis B virus (HBV) by secreting IL-21. However, the role of IL-21+CD4+ T cells in the immune response against chronic hepatitis C (CHC) virus infection is poorly understood. This study aimed to investigate the role of IL-21+CD4+ T cells in CHC patients and the potential mechanisms. The study subjects in-cluded nineteen CHC patients who were grouped by viral load (low, < 106 RNA copies/ml, n = 8; high, > 106 RNA copies/ml, n = 11). The peripheral frequency of HCV-specific IL-21+CD4+ T cells was higher in the low viral load group and was negatively correlated with the serum HCV RNA viral load in all CHC patients. Meanwhile, IL-21+ cells accumulated in the liver in the low viral load group. In vitro, IL-21 treatment increased the expression of proliferation markers and cytolytic molecules on HCV-specific CD8+ T cells. In summary, these findings suggest that HCV-specific IL-21+CD4+ T cells might contribute to HCV control by rescuing HCV-specific CD8+ T cells in CHC patients.

Model updating with constrained unscented Kalman filter for hybrid testing

Wu, Bin,Wang, Tao Techno-Press 2014 Smart Structures and Systems, An International Jou Vol.14 No.6

The unscented Kalman filter (UKF) has been developed for nonlinear model parametric identification, and it assumes that the model parameters are symmetrically distributed about their mean values without any constrains. However, the parameters in many applications are confined within certain ranges to make sense physically. In this paper, a constrained unscented Kalman filter (CUKF) algorithm is proposed to improve accuracy of numerical substructure modeling in hybrid testing. During hybrid testing, the numerical models of numerical substructures which are assumed identical to the physical substructures are updated online with the CUKF approach based on the measurement data from physical substructures. The CUKF method adopts sigma points (i.e., sample points) projecting strategy, with which the positions and weights of sigma points violating constraints are modified. The effectiveness of the proposed hybrid testing method is verified by pure numerical simulation and real-time as well as slower hybrid tests with nonlinear specimens. The results show that the new method has better accuracy compared to conventional hybrid testing with fixed numerical model and hybrid testing based on model updating with UKF.

Identification of drought response genes in Zygophyllum xanthoxylum by suppression subtractive hybridization

Bin Wu,Xiaohua Su 한국식물학회 2016 Journal of Plant Biology Vol.59 No.4

Drought is an important abiotic stress that seriously limits plant growth and crop productivity in some regions of the world. Zygophyllum xanthoxylum, a succulent xerophyte species with excellent adaptability to adverse arid environments, is an excellent model system for the study of the molecular mechanisms behind drought tolerance in xerophytic species. To better understand the molecular basis of Z. xanthoxylum responses to drought stress and to isolate drought tolerance-related genes from Z. xanthoxylum, we constructed a cDNA library from Z. xanthoxylum and screened the library for stress-related genes by PCR-based suppression subtractive hybridization (SSH). A total of 637 expressed sequence tags were generated by sequencing 672 randomly selected clones, resulting in the identification of 338 unigenes comprising 77 contigs and 261 singlets. The unigenes were assigned to 13 functional categories, such as metabolism, disease- or defense-related genes and energyrelated gene. In addition, we analyzed the expression patterns of 24 genes associated with drought stress-responsive pathways by real-time RT-PCR. The results indicated that osmolyte biosynthesis genes, reactive oxygen scavengers, transporters, signaling components and transcription factors play important roles in drought stress responses. This study provides a valuable resource for studying the drought tolerance mechanism of this xerophytic plant species.

Study on Crystallization Kinetics of Dynamically-Vulcanized PP/EPDM Blends

Bin Yang,Yan-Li Deng,Xia Ru,Ji-Bin Miao,Ming Cao,Jia-Sheng Qian,Li-Fen Su,Peng Chen,Jing-Wang Liu,La-Xia Wu,Tao Pang 한국고분자학회 2016 폴리머 Vol.40 No.4

Two types of β nucleating agents (β-NAs), aryl dicarboxylic acid amide (TMB-5) and diphenyl phthalate diamine (NT-C), were adopted to modify the polypropylene (PP)/ethylene propylene diene monomer (EPDM) blends, which were prepared by dynamic-vulcanization technology. Wide angle X-ray diffraction (WAXD) and differential scanning calorimetry (DSC) were used to study the crystallization kinetics of PP. Our results showed that the addition of β-NAs can considerably increase the crystallization temperature, and significantly decrease the spherulite size of β-PP (L300). The Jeziorny analysis showed there were ~82% and ~89% of relative crystallinity generated from the primary crystallization in the composites containing TMB-5 and NT-C, respectively. The crystallization half time (t0.5) showed that NT-C improved the overall crystallization rate more effectively than TMB-5. In addition, the peaks of the relative crystallization rate curves were shifted towards higher temperature by 14 and 9℃ with the addition of TMB-5 and NT-C, respectively.

A management strategy for solar panel - battery - super capacitor hybrid energy system in solar car

Bin Wu,Fang Zhuo,Fei Long,Weiwei Gu,Yang Qing,YanQin Liu 전력전자학회 2011 ICPE(ISPE)논문집 Vol.2011 No.5

This paper presents an application of solar energy - battery - super-capacitor hybrid energy storage system in solar electric vehicles. The key point is the proposed energy management control algorithm. The entire system consists of a solar panel, a boost converter, a battery, a super capacitor, a bi-directional DC/DC converter, and a brushless DC motor. This paper gives the details of the control strategy to cooperate the three energy sources or storage devices: solar panel, battery and super capacitor. A hysteretic control algorithm for battery charging by solar panel is given. A novel control method for bi-directional DC/DC is proposed to keep the battery discharging current within a certain limit and make full use of super capacitor. The switching transient between super-capacitor charging mode and discharging mode is also presented. Both simulation and experimental results demonstrate the effectiveness of the proposed strategy.

Novel flexible styrenic elastomer cation-exchange material based on phenyl functionalized polystyrene-butadiene copolymer

Wu Bin Ying,장지욱,이민우,황택성,이경진,이범재 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.47 No.-

The sulfonated phenyl functionalized polystyrene-butadiene random copolymer was synthesized forapplication asflexible ion-exchange material. The polymer was prepared through phenyl functionalizationvia thiol-ene reaction and sulfonation of the base polystyrene-butadiene copolymer with differentvinyl contents and phenyl contents. Sulfonation on phenyl functionalized polystyrene-butadienecopolymer was carried out in 1,2-dichloroethane at room temperature using the acetyl sulfonate as thesulfonation agent which was prepared from the reaction of acetyl acetate with sulfuric acid. Thesulfonation degrees were adjusted from 7.3 mol% to 24.3 mol% determined by elemental analysis (EA)with great ion exchange capacity (0.60–2.26 meq/g), conductivity (0.47–6.1 mS/cm) and lower wateruptake (5.1–12.8 wt.%). These excellent characteristics lead to great potential using as theflexibleion-exchange material.

Model updating with constrained unscented Kalman filter for hybrid testing

Bin Wu,Tao Wang 국제구조공학회 2014 Smart Structures and Systems, An International Jou Vol.14 No.6

The unscented Kalman filter (UKF) has been developed for nonlinear model parametric identification, and it assumes that the model parameters are symmetrically distributed about their mean values without any constrains. However, the parameters in many applications are confined within certain ranges to make sense physically. In this paper, a constrained unscented Kalman filter (CUKF) algorithm is proposed to improve accuracy of numerical substructure modeling in hybrid testing. During hybrid testing, the numerical models of numerical substructures which are assumed identical to the physical substructures are updated online with the CUKF approach based on the measurement data from physical substructures. The CUKF method adopts sigma points (i.e., sample points) projecting strategy, with which the positions and weights of sigma points violating constraints are modified. The effectiveness of the proposed hybrid testing method is verified by pure numerical simulation and real-time as well as slower hybrid tests with nonlinear specimens. The results show that the new method has better accuracy compared to conventional hybrid testing with fixed numerical model and hybrid testing based on model updating with UKF.

Synthesis of Clickable Poly[2-(3-butenyl)-2-oxazoline]/Nylon-6,6 Fiber

Wu Bin Ying,Sohee Kim,Min Woo Lee,Kyung Jin Lee,Bum Jae Lee 한국고분자학회 2016 한국고분자학회 학술대회 연구논문 초록집 Vol.41 No.2

Co-amplification at Lower Denaturation-temperature PCR Combined with Unlabled-probe High-resolution Melting to Detect KRAS Codon 12 and 13 Mutations in Plasma-circulating DNA of Pancreatic Adenocarcinoma Cases

Wu, Jiong,Zhou, Yan,Zhang, Chun-Yan,Song, Bin-Bin,Wang, Bei-Li,Pan, Bai-Shen,Lou, Wen-Hui,Guo, Wei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Background: The aim of our study was to establish COLD-PCR combined with an unlabeled-probe HRM approach for detecting KRAS codon 12 and 13 mutations in plasma-circulating DNA of pancreatic adenocarcinoma (PA) cases as a novel and effective diagnostic technique. Materials and Methods: We tested the sensitivity and specificity of this approach with dilutions of known mutated cell lines. We screened 36 plasma-circulating DNA samples, 24 from the disease control group and 25 of a healthy group, to be subsequently sequenced to confirm mutations. Simultaneously, we tested the specimens using conventional PCR followed by HRM and then used target-DNA cloning and sequencing for verification. The ROC and respective AUC were calculated for KRAS mutations and/or serum CA 19-9. Results: It was found that the sensitivity of Sanger reached 0.5% with COLD-PCR, whereas that obtained after conventional PCR did 20%; that of COLD-PCR based on unlabeled-probe HRM, 0.1%. KRAS mutations were identified in 26 of 36 PA cases (72.2%), while none were detected in the disease control and/or healthy group. KRAS mutations were identified both in 26 PA tissues and plasma samples. The AUC of COLD-PCR based unlabeled probe HRM turned out to be 0.861, which when combined with CA 19-9 increased to 0.934. Conclusions: It was concluded that COLD-PCR with unlabeled-probe HRM can be a sensitive and accurate screening technique to detect KRAS codon 12 and 13 mutations in plasma-circulating DNA for diagnosing and treating PA.

Nonlinear resonance of porous functionally graded nanoshells with geometrical imperfection

Wu-Bin Shan,Gui-Lin She 국제구조공학회 2023 Structural Engineering and Mechanics, An Int'l Jou Vol.88 No.4

Employing the non-local strain gradient theory (NSGT), this paper investigates the nonlinear resonance characteristics of functionally graded material (FGM) nanoshells with initial geometric imperfection for the first time. The effective material properties of the porous FGM nanoshells with even distribution of porosities are estimated by a modified power-law model. With the guidance of Love’s thin shell theory and considering initial geometric imperfection, the strain equations of the shells are obtained. In order to characterize the small-scale effect of the nanoshells, the nonlocal parameter and strain gradient parameter are introduced. Subsequently, the Euler-Lagrange principle was used to derive the motion equations. Considering three boundary conditions, the Galerkin principle combined with the modified Lindstedt Poincare (MLP) method are employed to discretize and solve the motion equations. Finally, the effects of initial geometric imperfection, functional gradient index, strain gradient parameters, non-local parameters and porosity volume fraction on the nonlinear resonance of the porous FGM nanoshells are examined.