효모에서 분리한 단백 메틸레스의 특성에 관한 연구

김보경,백운기,조만희,김창세 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

A cytochrome C - specific protein methylase Ⅲ was purified approximately 63 - fold from Saccharomyces cerevisiae. The unmethylated cytochrome C served as substrate for the enzyme. Horse heart apocytochrome C was found to be a better substrate than the native horse heart cytochrome C. This observation suggests that the in vivo methylation of cytochrome C in the yeast probably occurs before cytochrome C become attached to the mitochondria. In partly purified protein methylase Ⅲ the optimun pH is about 9.0, the enzyme activity is linearly proportional to the amount of enzyme protein up to about 30㎍ and also to time up to 15min. The Km values for horse heart cytochrome C and S - adenosyl - L- methionine were 1.33mM and 40㎛, respectively. S - adenosyl - L - homocysteine was found to be a competitive inhibitor for S - adenosyl - L - methionine, and the Ki value was 2.7 μM. The molecular weight of the enzyme was approximately 97,000, determined on sephadex S- 200 gel filtration. The regulation of cellular cytochrome C and the protein methylase Ⅲ are not coordinately related in a parallel manner.

Endogenous Proteinaceous Inhibitor for Protein Methylation Reactions

Paik, Woon-Ki,Lee, Hyang-Woo,Kim, Sangduk The Pharmaceutical Society of Korea 1987 Archives of Pharmacal Research Vol.10 No.3

Protein methylation occurs ubiquitously in nature and involves N-methylation of lysine, arginine, histidine, alanine, proline and glutamine, O-methylesterfication o dicarboxylic acids, and S-methylation of cysteine and methionine. In nature, methylated amino acids accur in highly specialized proteins such as histones, flagella proteins, myosin, actin, ribosomal proteins. hn RNA-bound protein, HMG-1 and HMG-2 protein, opsin, EF-Tu, EF-$1\alpha$, porcine heart citrate synthase, calmodulin, ferredoxin, $1\alpha$-amylase, heat shock protein, scleroderma antigen, nucleolar protein C23 and IF-3l.

A Possible Influence of a Single Methyl Substitution on the Tertiary Structure of a Protein Molecule

Paik, Woon Ki,]Kim, Sangduk 한국생화학분자생물학회 1991 생화학분자생물학회 소식 Vol.11 No.4

Biological effects caused by the posttranslational methylation of certain side chains in proteins has been thought to be due solely to changes in charge, steric relations or hydrophobicity at the site of the methyl group. However, there is increasing evidence that the presence of CH, can also induce a "global" effect on the protein molecule. Some of the evidence is described here.

Protein Methylation and Interaction with the Antiproliferative Gene, BTG2/TIS21/Pc3

Woon Ki Paik,Sangduk Kim,임인경 연세대학교의과대학 2014 Yonsei medical journal Vol.55 No.2

The last one and half a decade witnessed an outstanding re-emergence of attention and remarkable progress in the field of protein methylation. In the present article, we describe the early discoveries in research and review the role protein methylation played in the biological function of the antiproliferative gene, BTG2/TIS21/PC3.

A Possibls Influence of a Single Methyl Substitution on the Tertiary Structure of a Protein Molecule

Paik, Woon Ki,Kim, Sangduk 성균관대학교 약학연구소 1990 成均藥硏論文集 Vol.2 No.1

Abstract-Biological effects caused by the posttranslational methylation of certain side chains in protein has been thought to be due solely to changes in charge, steric relations or hydrophobicity at the site of the methyl group. However, there is increasing evidence that the presence of CH_3 can also incuce a “global”effect on the protein molecule. Some of the evidence is described here.

Enzymology of Protein Methylation

Paik, Woon Ki,Kim, Sangduk Yonsei University, College of Medicine 1986 Yonsei medical journal Vol.27 No.3

일반논문 1 : 김종익의 유언과 경성여자의학전문학교 설립과정

백운기 ( Woon Ki Paik ),김상덕 ( Sang Duk Kim ) 연세대학교 의과대학 의사학과 의학사연구소 2011 연세의사학 Vol.14 No.1

Dr. Rosetta Hall, an American missionary physician, and Dr. Jeong-hee Gil, a young Korean physician, founded the Joseon (Keijo) Women`s Training Institute in 1928. Between 1933 and 1937, Dr. Gil and her husband, Dr. Kim Tak-won, maintained and financed the medical institute. Supporting the institute placed a heavy burden on the young doctors who were just establishing their private medical practice. Despite this burden, they undertook the work necessary to elevate the institute to a full medical college. In order to generate the substantial funds needed to establish the medical college, they created a foundation for the “creation of a women`s medical college” in 1934 and solicited funding. In 1937, a philanthropist interested in furthering education, Mr. Kim Jong-ik, agreed to donate the funding necessary to elevate the institute to a medical college. Mr. Kim, however, unexpectedly contracted dysentery and died. In his will, Mr. Kim bequeathed a portion of his estate to upgrading the institute to a medical college. The executor, contrary to Mr. Kim`s intent as set forth in his will, however, did not use the funds to elevate the institute, but rather established a completely new women`s medial college. The executor`s actions were a clear violation of Drs. Kim`s and Gil`s legal rights as beneficiaries under the will. They, nonetheless, accepted the outcome, because challenging the executor`s actions under Japanese rule would have been futile as Dr. Kim was a noted anti-Japanese patriot well known to the Japanese. Moreover, Sato Gozo had been Dr. Kim`s teacher at the Keijo Medical College. Most importantly, their dream of establishing a women`s medical college in Korea had been realized regardless of how. Regardless of whether the institute had been elevated to a medical college or not, Drs. Kim Tak-won and Gil Jeong-hee made great sacrifices to further the education of women medical doctors in Korea and should be recognized for their great contributions to the creation of Korea Women`s Medical College.

Control of the Rice Stem Borer(Chilo suppressalis) with the new Insecticides

Paik Woon Hah,Choi Seung Yoon,Yun Soon Ki,Im Moon Soon Korean Society of Applied Entomology 1962 한국식물보호학회지 Vol.1 No.-

1. 본시험은 이화곤충방제에 유효하다고 인정된 신살충제를 선정하여 이화곤충에 대한 살충효과의 재확인 및 살포적기를 구명코저 시행하였다. 2. 제일화기에 있어서 Diazinon, Lebaycild, Endrin 및 Folidol은 부화 14일전 처리로서도 부화유충의 식입제로서 유효하였고, 부화유충 식입후일처리구에 있어서도 무처리구에 비하여 유효하였다. 3. 제2화기에 있어 부화 21일전 처리구의 유효성은 Detron=Folidol>$\gamma$-dol>Endrin>Dimecron의 순이었다. 그리고 부화 14일 및 17일전과 부화 2일 및 7일후 처리에 있어서도 $\gamma-dol$을 제한 다음 약제들은 고도의 유효성을 나타내었다. 4. 그러나 약제처리시기 사이에는 유의성이 없었다. 5. 본시험은 1회의 시험성적임으로 동일시험이 수회반복되어 살충효과 및 살포적기가 완전히 구명되었으면 한다.

Inhabiting Condition and Preservation of Black-faced Spoonbill around Ganghwa County

Paik, In-Hwan,Chun, Byung-Sun,Kim, In-Gyu,Lee, Ki-Sup,Jin, Seon-Deok,Yu, Jae-Pyung,Lee, Han-Soo,Paek, Woon-Kee national science museum of korea 2010 Journal of Korean nature Vol.3 No.1

Regular monitoring on the Black-faced Spoonbill and survey on their breeding were performed in the vicinity of Ganghwa County from August 2002 till July 2003 in order to understand the inhabiting conditions of the endangered species of the bird (EN) defined by IUCN. Maximum 230 individuals of the Black-faced Spoonbill around the County were observed at the southern tidal flat of Ganghwa, which was the largest and 51 at Gyodongdo Island, 55 at Boreumdo Island while one at Seokmodo Island. No Black-faced Spoonbill was observed at Jumundo Island. For the inhabiting status of the bird as per the time of survey, 203 individuals were observed in August 2002, which was recorded as the largest of its kinds, on the other hand, only 5 individuals were found in March, which was the least. No Black-faced Spoonbill was observed from November till February of the following year. For the areas of survey, more individuals of the endangered bird were observed at the southern tidal flat of Ganghwa during the period of survey than other areas and it is because the bird took a rest at the southern tidal flat of Ganghwa and fed their foods. Breeding of the bird was verified at Yudo Island, Seokdo Island, Bido Island and Suribongdol Island and the breeding status as per each island is 100 individuals at Yudo Island, 10 at Seokdo Island, 8 at Bido Island and 5 at Suribongdol Island. On the other hand, the Black-faced Spoonbill found at the southern tidal flat of Ganghwa fed their foods at the nearby rice paddy during May and June. Heavy rainfall in 2007 and the pressure on developing the area surrounding Ganghwdo Island may force the endangered birds to breed at the artificial island of the Incheon Songdo International City or the main inhibiting place is in danger of vanishing. In order to protect the Black-faced Spoonbill, therefore, development and use of not only the tidal flat but also the rice paddy that is the wetland with fresh water and shallow tidal flat should be minimized. Also, converting to the eco-friendly development plan of the reclaimed land and collecting information to restore the inhabiting place of Black-faced Spoonbill is essential.

A List of Spiders Collected in the Rice Paddy at Milyang

Woon Hah Paik,Jun Namkoong,Hak Ki Kim 한국응용곤충학회 1974 한국응용곤충학회지 Vol.13 No.1

전적적으로 널리 재배되고 있는 통일(IR667-98)이 우리나라의 현존 도열병균 Race이 대하여 고도로 저항성을 유지하고 있다. 그러나 통일과 그 자매계통은 1972년 서울대학교 도열병검정밭못자리에서 통일에서 분리한 RaceIA-65에 대해 중도감수성이었다. 1973년에는 IA-65를 접종한 격리밭못자러에서 통일을 비롯한 IR계통은 이 Race를 접종하지 않은 농장못자리보다 현저히 병원성이 높았다. 또한 IA-65에 대한 196 IR 계통의 유묘점정반응은 통일을 포함한 약 가 중도감수성이었다. 1973년에 다시 통일을 비롯한인도형 계통에서 주로 분리한 30균주중 12개는 통일이 고도감수성이었던 필리핀 국제미작연구소의 우점 Race 군으로 알려진 IA군으로 판별되었다. Race IA군도 유묘검정에서 통일에 병원성이 있었다. 이와같이 통일 및 IR 계통에 병원성인 Race IA 65 및 IA군은 한국을 비롯한 일본형 벼재배지대에서 보고된 바 없는 새로운 것으로 생각된다. 우리나라에 현존하는 몇 가지 Race가 통일에 병원성을 보이고 있고 더 나아가서 이들 Race가 일반 농도포장에서 만연될지도 모르므로 통일에 대한 도열병의 반응이 계속 추구되어야 한다. Since Tongil has been cultivated widely in farmers' fields, the variety is known to be highly resistant to existing races of Pyricularia oryzae in Korea. However, Tongil and the sister lines were moderately susceptible to race IA-65, which was isolated from Tongil at the blast nursery of the College of Agriculture, Seoul National University, 1972. In field tests of 1973, pathogenic reactions of IR lines at the isolated nursery, where IA-65 had been inoculated, were significantly higher than those at the farm nursery. When seedlings of 196 IR lines were inoculated with IA-65, about of the IR lines including IR667-98 were moderately susceptible. Among the 30 isolates of P. oryzae obtained mostly from indicas, 12 were also differentiated in 1973 as IA group known to be the most prevalent races at IRRI in the Philippines, where Tongil has been highly susceptible. This IA group was also pathogenic to Tongil in greenhouse experiments. The race group causing moderately susceptible to Tongil should be new in Korea, since no occurrences of the race IA have ever been reported previously from the areas where japonica types of rice are cultivated. Because some races of P. oryzae present in Korea are pathogenic to Tongil and because these races may become prevalent in the fields, the reaction of Tongil must be continuously monitored.