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Targeting Orthotopic Glioma in Mice with Genetically Engineered Salmonella typhimurium
Wen, Min,Jung, Shin,Moon, Kyung-Sub,Jiang, Shen Nan,Li, Song-Yuan,Min, Jung-Joon The Korean Neurosurgical Society 2014 Journal of Korean neurosurgical society Vol.55 No.3
Objective : With the growing interests of bacteria as a targeting vector for cancer treatment, diverse genetically engineered Salmonella has been reported to be capable of targeting primary or metastatic tumor regions after intravenous injection into mouse tumor models. The purpose of this study was to investigate the capability of the genetically engineered Salmonella typhimurium (S. typhimurium) to access the glioma xenograft, which was monitored in mouse brain tumor models using optical bioluminescence imaging technique. Methods : U87 malignant glioma cells (U87-MG) stably transfected with firefly luciferase (Fluc) were implanted into BALB/cAnN nude mice by stereotactic injection into the striatum. After tumor formation, attenuated S. typhimurium expressing bacterial luciferase (Lux) was injected into the tail vein. Bioluminescence signals from transfected cells or bacteria were monitored using a cooled charge-coupled device camera to identify the tumor location or to trace the bacterial migration. Immunofluorescence staining was also performed in frozen sections of mouse glioma xenograft. Results : The injected S. typhimurium exclusively localized in the glioma xenograft region of U87-MG-bearing mouse. Immunofluorescence staining also demonstrated the accumulation of S. typhimurium in the brain tumors. Conclusion : The present study demonstrated that S. typhimurium can target glioma xenograft, and may provide a potentially therapeutic probe for glioma.
Wen-Ming Chen,Yi Min Xie,Gabriele Imbalzano,Jianhu Shen,Shanqing Xu,이성재,Peter Vee Sin Lee 한국정밀공학회 2016 International Journal of Precision Engineering and Vol.17 No.6
The development of porous metals to alleviate the effects of stress shielding in bone will help improve the function of metallic biomaterials in orthopaedic applications. A critical step in advancing this technology is to design metallic structures with low rigidity that is comparable with bone tissue, but with good mechanical strength. In this study, porous titanium (Ti) structures with periodic cell topologies were designed to achieve tunable mechanical properties. The versatility of the design scheme was demonstrated by examining lattice designs with different stiffness properties achieved by using the Selective Laser Melting (SLM) technology. The fabricated porous Ti exhibited a low modulus of 1.05 GPa but a high compressive strength of 55 MPa. Large deformation analysis using digital image correlation (DIC) technique indicated uniform strain patterns at micro-trusses, suggesting the overall high quality of the structure with absence of local flaws. A functionally-graded stiffness design was further investigated by varying the diameters of micro-trusses within the structure. A stiffness graded material may be favourable for anatomical site that has strong depthdependent variations, such as in trabecular bone microstructures.
Identification of a Novel Human Zinc Finger Gene, ZNF438, with Transcription Inhibition Activity
( Zhao Min Zhong ),( Bo Wan ),( Yun Qiu ),( Jun Ni ),( Wen Wen Tang ),( Xin Ya Chen ),( Yun Yang ),( Su Qin Shen ),( Ying Wang ),( Mei Rong Bai ),( Qing Yu Lang ),( Long Yu ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.4
Feeding Value of High-oil Corn for Taiwan Country Chicken
Lin, Min-Jung,Chiou, Peter Wen-Shyg,Chang, Shen-Chang,Croom, Jim,Fan, Yang-Kwang Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.9
The feeding value of high-oil corn fed to Taiwan Country (TC) chicken was examined by measuring apparent metabolizable energy (AME), growth performance, sexual maturity, carcass characteristics, and plasma pigmentation. In a completely randomized design, 870 sex-intermingled one-wk-old chicks were assigned to one of 30 floor pens, 29 birds per pen, and each pen randomly assigned to one of five dietary treatments. The experiment was ended when birds were 16 wk of age. The five dietary treatments varied in main fat sources, which were corn oil (CO), high-oil corn (HOC), lard (LRD), whole soybean (WSB) and yellow corn (YC), respectively. All the diets were formulated isonitrogenously, isocalorically, and of equal lysine and methionine contents except YC, in which equal amounts of YC replaced HOC. The results indicated that feed conversion in HOC was 8% higher (p<0.05) than YC whereas the calculated AME of HOC was only 3.5% to 4.0% higher than that of YC. No significant differences were observed in body weight, body weight gain, feed consumption, feed conversion ratio and ME efficiency for body weight gain among CO, HOC, LRD, and WSB. No significant differences existed in both skin and muscle pigmentation of breast among the five dietary treatments. No significance differences existed in plasma carotenoid content measured at various ages among the five dietary treatments except that birds fed with HOC had less (p<0.05) plasma carotenoids at 16 wk-old. The results indicate that if the price of high-oil corn is no more than 1.05 times that of yellow corn, the dietary cost per kg of body weight gain for TC chickens fed diets containing high-oil corn will be less, although their body weight may be lighter compared to chickens fed diets formulated with other fat sources.
( Jiao Wen Shen ),임윤희 ( Yun Hui Lim ),조영민 ( Young Min Jo ) 한국공업화학회 2011 공업화학 Vol.22 No.2
본 연구에서는 화학적 공침법을 적용하여 가스상 이산화탄소 분해를 위한 나노크기의 M-페라이트(M=Co, Ni, Cu, Zn)를 제조하였다. 열중량 분석 결과, 시험제조한 모든 시료의 최고 무게 감소율은 350 ℃ 미만에서 발생하였다. 소성온도가 증가할수록 결정형은 우수하여 표면촉매활성화를 기대할 수 있지만, 입자결정의 크기가 크고, 비표면적이 낮은 페라이트가 합성됨을 알 수 있었다. FT-IR 분석으로부터 375~406 cm-1의 범위에서 octahedral site에 착화물이 존재함을 확인 할 수 있었으며, 이는 페라이트 내 스피넬 구조가 형성되어 있음을 보여주는 것이라고 믿는다. 본 연구로부터 얻은 이산화탄소 분해반응을 위한 금속페라이트의 최적 열처리 온도는 500 ℃인 것으로 나타났다. In this work, nano-sized M-ferrites (M=Co, Ni, Cu, Zn) for the decomposition of carbon dioxide were synthesized by the chemical co-precipitation. From the thermogravimetric analysis, it was clear that the maximum weight loss of each sample took place below 350 ℃. High temperature calcination resulted in more systematic crystallines, smaller specific surface area and larger particle size. An analysis by FTIR in the range of 375~406 cm-1 revealed the presence of chelates at the octahedral site, which implies the formation of spinel structure in the ferrites. The current work showed that a 500 ℃ is the optimum heat treatment temperature of metal ferrites for CO2 decomposition reaction.
Cao,Jain Min,Wu,Chuan Liang,Shen,Wen ZhEng,Huang,Chang,Li,Yin Bo,Xu,Yang Zhen 대한전자공학회 1997 ICVC : International Conference on VLSI and CAD Vol.5 No.1
Start with 2D simulation of hot-carrier injection current. in this pier, we have discussed the influence of different silicon film thickness (Tsi), gate oxide thickness (Tox1) and substrate doping (Na) an the hot-carrier effects of thin-film deep submicron SOI/MOSFET. Simulation results indicate that, with reduction of Tsi, the hot-carrier current is reduced (by more than 2 orders); and in Tsi range of 0.05-0.1μ. the hot carrier carrent is smallest and insensitive to Tox1, Na and Tsi. These are helpful to the design of high reliability SOI/MOSFET.
Ruiyue Qiu,Wen Zhao,Jiao Yang,Yanwei Shen,Biyuan Wang,Pan Li,Andi Zhao,Qi Tian,Mi Zhang,Min Yi,Jin Yang,Danfeng Dong 한국유방암학회 2019 Journal of breast cancer Vol.22 No.2
Purpose: Numerous previous studies have reported inconsistent results about the differences between synchronous contralateral breast cancer (sCBC) and metachronous contralateral breast cancer (mCBC). This study aimed to compare the clinical characteristics and outcomes between sCBC and mCBC and determine predictive factors for the survival of sCBC and mCBC patients. Methods: Using the Surveillance, Epidemiology, and End Results Program database, we identified sCBC or mCBC patients from 2000 to 2010. The Kaplan-Meier method and Cox proportional hazards regression analysis were used to analyze overall survival and breast cancer-specific survival (BCSS) rates of sCBCs and mCBCs, respectively. Results: Overall, 14,057 sCBC (n = 8,139, 57.9%) and mCBC (n = 5,918, 42.1%) patients were included. The first tumors of sCBC were more likely to have higher stage and more lymph and distant metastases, whereas those of mCBC were more often infiltrating ductal carcinoma (IDC), had localized stage, were estrogen receptor (ER) and progesterone receptor (PR) negative, and had less axillary nodal involvement. The second tumors of mCBC tended to be IDC and have higher grade, adverse stage, ER and PR-negativity; and more axillary nodal involvement, compared to the second tumors of sCBC. mCBC patients had significantly favorable 5-year BCSS but worse long-term BCSS compared with sCBC patients. Moreover, subgroup analysis revealed no significant difference of BCSS between sCBC and mCBC among patients aged 18–60 years. Multivariate analysis indicated that age, grade, and stage of 2 tumors; surgery for second tumor; and ER status of the second tumor were independent prognostic factors for BCSS of contralateral breast cancer (CBC). Conclusion: The characteristics and outcomes of sCBCs and mCBCs were substantially different. sCBC and mCBC patients may have different prognosis, and the prognosis of CBC depends on the first and second tumors.