Genome-wide identification and analysis of expression pattern of REVEILLE transcription factors in castor (Ricinus communis L.)

Wei Xue,Zhang Jixing,Wang Nan,Sun Mengdi,Ding Xue,Xu Hui,Yu Xiumin,Yue Wenran,Huo Hongyan,Yu Lili,Wang Xiaoyu 한국원예학회 2024 Horticulture, Environment, and Biotechnology Vol.65 No.3

REVEILLE (RVEs) transcription factors play an important role in regulating circadian clock, plant growth and development, and stress responses. While RVEs have been identifi ed in various plant species, comprehensive genome exploration and analysis of RVEs in castor ( Ricinus communis L.) have not been previously reported. In this study, we identifi ed RcRVEs in the castor genome through sequence alignment and conducted an analysis encompassing gene structures, protein motifs, physicochemical properties, transcription patterns under cold stress, diff erent tissues, and diurnal rhythm. A total of fi ve RcRVEs were pinpointed in the castor genome. Evolutionary tree analysis categorized these RcRVEs into three distinct groups, implying that diff erent RcRVEs may fulfi ll distinct biological functions. Furthermore, several abiotic stresses and hormone-responsive cis -acting elements were found in the promoters of the RcRVEs . RNA-sequencing (RNA-seq) analysis revealed that RcRVE1 and RcRVE7 were induced by cold stress and exhibited upregulation over time, indicating their involvement in regulating cold adaptation in castor plants. Five RcRVEs have notably higher expression levels in leaves compared to other tissues. The expression of all RcRVEs displayed diurnal rhythms under light/dark cycles. This study provides valuable insights into the potential functions of RcRVEs in regulating castor’s growth and development, and responses to cold stress.

Analysis of Fracture Characteristics of Ore Rock Based on GMTS Criterion

Xiaoyu Tang,Wen Wan,Wei Chen,Zongtang Zhang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.10

In this paper, the Tongkaiyu ore-rock is taken as the research object, aiming to analyze the fracture characteristics of the mixed type I, II and I-II faults in ore-rock by using the appropriate fracture criterion, and analyze the fracture development mode. Considering the non-singular term (T stress) in the formula of stress field sequence at crack tip, the generalized maximum tangential stress (GMTS) criterion is established, which is consistent with the boundary conditions of the central straight Brazilian disk (CSTBD) specimen. Through CSTBD splitting test and three-dimensional particle flow code (PFC3D) numerical simulation, the Angle between the central direct joint and the loading direction was changed, and the fracture toughness and fracture development law of type I, type II and type I-II mixed fracture were obtained. The theoretical and simulation results show that, compared with the maximum tangential stress (MTS) criterion, the GMTS criterion has an advantage in judging the fracture characteristics of mixed types I, II and I-II in ore rock.

High-precision Position Tracking Control of Electro-hydraulic Servo Systems Based on an Improved Structure and Desired Compensation

Wei Shen,Xinyu Liu,Xiaoyu Su 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.11

How to improve the position tracking accuracy of electro-hydraulic servo system is a hot issue today. Full state feedback control has received widespread attention for its ability to significantly improve control performance,however, its practical application range is limited in view of the large influence of measurement noise. In terms of this issue, we propose an adaptive robust controller based on improved structure and desired compensation. Firstly,to reduce the impact of measurement noise, the actual state value is substituted by the corresponding desired value in the controller design based on model compensation and the adaptive model compensator. Then, we introducea new auxiliary variable into the controller to optimize its structure. In addition, nonlinear robust control laws are integrated in the controller to balance unstructured uncertainties. Simulation analysis shows that the proposedcontrol strategy not only achieves the asymptotic tracking when parameter perturbation exists, but also ensures a specified transient response and final tracing precision under the combined influence of structured and unstructureduncertainties. The results indicate that the control strategy has good control accuracy as well as strong robustness

Response of Ammonia Assimilation in Cucumber Seedlings to Nitrate Stress

Xiaoyu Yang,Xiufeng Wang,Min Wei,Shoko Hikosaka,Eiji Goto 한국식물학회 2010 Journal of Plant Biology Vol.53 No.3

The influence of increased nitrate concentration—14 (control) and 140 mmol L−1 (T)—in hydroponic culture on ammonia assimilation in cucumber (Cucumis sativus L. cv. Xintaimici) seedlings was investigated. The results showed that NH3 accumulation in the roots and leaves of T seedlings increased significantly, indicating that NH3toxicity might be involved in nitrate stress. Under control conditions, GS and GOGAT activity were much higher in the leaves than in the roots, whereas GDH activity was much higher in the roots than in the leaves. Correlation analysis showed that NH3 concentration had a strong negative linear relationship with GDH activity in the roots but had a strong negative linear relationship with GS and GOGAT activity in the leaves. These results indicate that NH3 might be assimilated primarily via GDH reaction in the roots and via GS/GOGAT cycle in the leaves. Shortterm nitrate stress resulted in the increase of GS and GOGAT activity in the roots and GDH activity in the leaves of T seedlings, indicating possible shifts in ammonia assimilation from the normal GDH pathway to GS/GOGAT pathway in the roots and from the normal GS/GOGAT pathway to the GDH pathway in the leaves under nitrate stress, but with the increase of treatment time, GS, GOGAT,and GDH activity in the roots and leaves of T seedlings decreased possibly due to low water potential and NH3toxicity.

Carbon disulfide induces mitochondria-mediated apoptosis in Sertoli-germ cells coculture

Wei Wang,Zhen Zhang,Yinsheng Guo,Yu Dong,Xiaoyu Huang1,Yijun Zhou,Guoyuan Chen 대한독성 유전단백체 학회 2015 Molecular & cellular toxicology Vol.11 No.2

Carbon disulfide (CS2), a common organic solvent, induces a variety of adverse effects in the male reproductive system. In this study, we investigated the cytotoxicity, ultrastructural changes, and potential apoptotic induction mechanisms of CS2 in mixed cultures of Sertoli and germ cells. Sertoli and germ cells were cocultured and treated with CS2 for 24 h. Growth rates were noted, and apoptotic cells were identified by Hoechst 33258 staining. Ultrastructure changes were observed via transmission electron microscopy (TEM). Mitochondrial membrane potential and expressions of apoptosis-related factors (cytochrome c, Bax, Bcl- 2, caspase-3 and caspase-9) were examined by JC-1 staining, western blot, and real-time PCR. The results showed that CS2 treatment was associated with reduced growth rates of Sertoli-germ cells. Ultrastructure changes in Sertoli-germ cells treated with CS2 were typical of apoptosis. In addition, CS2 treatment depolarized mitochondrial membrane potential, upregulated Bax levels and downregulated Bcl-2 levels, released cytochrome c from the mitochondrial intermembrane space to the cytosol, and triggered mitochondria-mediated apoptosis. Subsequently, caspase-9 and caspase-3 were activated, resulting in Sertoli-germ cells apoptosis. The above data suggest that CS2 has adverse effect on the viability of Sertoli-germ cells and induces apoptosis through mitochondrial pathway.

Numerical analysis of the thermal behaviors of cellular concrete

Wei She,Guotang Zha,Guotao Yang,Jinyang Jiang,Xiaoyu Cao,Yi Du 사단법인 한국계산역학회 2016 Computers and Concrete, An International Journal Vol.18 No.3

In this study, both two- and three-dimensional (2D and 3D) finite-volume-based models were developed to analyze the heat transfer mechanisms through the porous structures of cellular concretes under steady-state heat transfer conditions and to investigate the differences between the 2D and 3D modeling results. The 2D and 3D reconstructed pore networks were generated from the microstructural information measured by 3D images captured by X-ray computerized tomography (X-CT). The computed effective thermal conductivities based on the 2D and 3D calculations performed on the reconstructed porous structures were found to be nearly identical to those evaluated from the 2D cross-sectional images and the 3D X-CT images, respectively. In addition, the 3D computed effective thermal conductivity was found to agree better with the measured values, in comparison with the 2D reconstruction and real cross-sectional images. Finally, the thermal conductivities computed for different reconstructed porous 3D structures of cellular concretes were compared with those obtained from 2D computations performed on 2D reconstructed structures. This comparison revealed the differences between 2D and 3D image-based modeling. A correlation was thus derived between the results of the 3D and 2D models.

Modeling and Verification of a Contactless Air Film Conveyor Using a Viscous Traction Principle

Wei Zhong,Xiaoyu Gu,Ke Xu,Fanghua Liu,Xin Li,Toshiharu Kagawa 한국정밀공학회 2017 International Journal of Precision Engineering and Vol.18 No.12

Many industries require contactless transport of delicate or clean products such as silicon wafers, flat foodstuffs and freshly painted objects. In this study, a contactless air film conveyor for flat objects is introduced. The object is supported by a thin film formed between the object and the conveyor surface and transported by viscous traction which is generated by controlled airflow underneath the object. Experiments are conducted to investigate the film pressure distribution and viscous force. The results show that the film pressure is symmetrically distributed along the direction perpendicular to the airflow in the actuating cells and non-symmetrically distributed along the airflow direction. The viscous force decreases as the increase of gap thickness. A simplified model is established to help understand and characterize the viscous force. The calculated results show a good agreement with the experimental data. It is revealed that the viscous force is the resultant of an actuating force in the pocket and side areas and a drag force from airflow across the dam area. Simulation and experiments are conducted using a PID controller for one dimension position control and position tracking. The results verify the effectiveness of the theoretical modeling and control method.

Investigating the Influences of Wet Fiber Laser Cutting Upon the Surface Integrity of Nitinol Cardiovascular Stents

Xiaoyu Sun,Xiuting Wei,Zhiyong Li,Deda Lou,Yongqi Wang,Hanqing Liu 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.22 No.7

High precision laser cutting with minimal post-treatment is needed in the manufacturing of nitinol cardiovascular stents. This paper carries out a series of cutting experiments of nitinol cardiovascular stents by wet fiber laser cutting to understand the basic process characteristics and to investigate the influences of process parameters (laser power, cutting speed, pulse width, frequency) upon the surface integrity (surface topography, dross, surface roughness, HAZ, recast layer, subsurface nanohardness, surface chemical composition). The univariate analysis was used to analyze and explain the influence that rules of process parameters had upon the surface integrity. The experiment results showed that the effects of process parameters on surface integrity were regular, with the optimal single-factor parameters obtained. Moreover, the removal mechanism of the nitinol material was also revealed. This study is of great significance for the medical industry in improve the cutting quality of cardiovascular stents.

The Combination of Platelet Rich Plasma Gel, Human Umbilical Mesenchymal Stem Cells and Nanohydroxyapatite/polyamide 66 Promotes Angiogenesis and Bone Regeneration in Large Bone Defect

Liu Wei,Huang Yong,Liu Daqian,Zeng Teng,Wang Jingzhe,Li Ang,Wang Dawei,Wang Xiaoyu 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.6

BACKGROUND: In the present study, a novel tissue engineering bone graft including platelet rich plasma gel (PRP gel), human umbilical mesenchymal stem cells (HUMSCs) and nanohydroxyapatite/polyamide 66 (nHA-PA66) was constructed. We explored whether the composite scaffolds could enhance the angiogenesis and bone repair capacity in rat femoral large bone defect (LBD). This study aimed to provide evidence for the clinical application of the composite scaffold in LBD treatment. METHODS: PRP was prepared, the platelets and growth factors were measured. HUMSCs were isolated and identified. the osteogenic capacity of PRP in vitro was measured. Then HUMSCs-PRP-gel/nHA-PA66 composite scaffolds were synthesized and observed. The proliferation and osteogenesis differentiation of HUMSCs on the composite scaffold was measured. The angiogenic capacity of PRP in vitro was measured by capillary-like tube formation assay. Finally, the angiogenesis and bone repair capacity of the composite scaffolds was measured in rat LBD. RESULTS: PRP contained high level of platelets and growth factors after activation, and promoted osteogenic and angiogenic differentiation in vitro. The HUMSCs-PRP-gel/nHA-PA66 composite scaffold was porosity and promoted the proliferation and osteogenesis differentiation of HUMSCs. At 12th weeks, more micro-vessels and new bone were formed around the composite scaffolds compared with other groups, the defect was almost repaired. CONCLUSION: Our study for the first time identified that the combination of PRP gel, HUMSCs and nHA-PA66 scaffold could significantly promote angiogenesis and bone regeneration in rat LBD, which may have implications for its further application in clinical LBD treatment.

团体沙盘对大学生团体依恋和孤独感的干预效果研究

莫晓月(Xiaoyue Mo),姚贤芬(Xianfen Yao),钟思倪(Sini Zhong),许雅婷(Yating Xu),凌威(Wei Ling),周彩虹(Caihong Zhou) YIXIN 출판사 2024 教育教学研究论丛 Vol.2 No.6

This study aimed to investigate the effects of group sandtable intervention on group attachment and loneliness among college students in order to enhance their physical and mental well-being as well as improve their core literacy. Twenty-four subjects were recruited and divided into experimental and control groups. The experimental group received eleven sessions of group sandbox interventions while the control group did not undergo any measures. Group Attachment Scale (GAS) and Loneliness Scale (UCLA) were used for data evaluation with paired T-test for analysis. The results showed a significant decrease in both group attachment avoidance and loneliness compared to pre-test levels (P < 0.05). Group sandplay can effectively reduce group attachment avoidance and loneliness among college students while enhancing their overall core quality. 本研究旨在探讨团体沙盘对大学生团体依恋和孤独感的干预效果,以促进大学生身心健康,提升核心素养。研究对象为公开招募24 位被试,均分为实验组和对照组,实验组开展共11 次的团体沙盘干预,对照组不采取任何措施。本研究采用团体依恋量表(GAS)和孤独感量表(UCLA)评估为研究工具,使用SPSS 23 进行数据分析。研究发现团体依恋回避及孤独感后测结果均显著低于前测(P<0.05),团体沙盘游戏可以改善大学生团体依恋回避和孤独感,有效提升大学生核心素养。