FABRICATION OF NANOPOROUS CHITOSAN MEMBRANES

XIAOLIANG WANG,XIANG LI,ELEANOR STRIDE,MOHAN EDIRISINGHE 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2010 NANO Vol.5 No.1

Naturally derived biopolymers have been widely used for biomedical applications such as drug carriers, wound dressings, and tissue engineering scaffolds. Chitosan is a typical polysaccharide of great interest due to its biocompatibility and film-formability. Chitosan membranes with controllable porous structures also have significant potential in membrane chromatography. Thus, the processing of membranes with porous nanoscale structures is of great importance, but it is also challenging and this has limited the application of these membranes to date. In this study, with the aid of a carefully selected surfactant, polyethyleneglycol stearate-40, chitosan membranes with a well controlled nanoscale structure were successfully prepared. Additional control over the membrane structure was obtained by exposing the suspension to high intensity, low frequency ultrasound. It was found that the concentration of chitosan/surfactant ratio and the ultrasound exposure conditions affect the structural features of the membranes. The stability of nanopores in the membrane was improved by intensive ultrasonication. Furthermore, the stability of the blended suspensions and the intermolecular interactions between chitosan and the surfactant were investigated using scanning electron microscope and Fourier transform infrared spectroscopy (FTIR) analysis, respectively. Hydrogen bonds and possible reaction sites for molecular interactions in the two polymers were also confirmed by FTIR analysis.

A Novel MAP Kinase Gene in Cotton (Gossypium hirsutum L.), GhMAPK, is Involved in Response to Diverse Environmental Stresses

Wang, Meimei,Zhang, Ying,Wang, Jian,Wu, Xiaoliang,Guo, Xingqi Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.3

The mitogen-activated protein kinase (MAPK) cascade is one of the major and evolutionally conserved signaling pathways and plays pivotal role in the regulation of stress and developmental signals in plants. Here, a novel gene, termed Gossypium hirsutum MAPK (GhMAPK), was isolated from cotton. The full-length cDNA of GhMAPK encodes for a 372 amino acid protein that contains all 11 of the MAPK conserved subdomains and the phosphorylationactivation motif, TEY. Amino acid sequence alignment revealed that GhMAPK shared high identity with group-C MAPK in plants and showed 83~89% similarities with MAPKs from Arabidopsis, apricot, pea, petunia, and tobacco. Southern blot analysis indicated that the GhMAPK belonged to a multygene family in cotton. Two introns were found within the region of genomic sequence. Northern blot analysis revealed that the transcripts of GhMAPK accumulated markedly when the cotton seedlings were subjected to various abiotic stimuli such as wounding, cold (4$^{\circ}C$), or salinity stress; Furthermore, GhMAPK was upregulated by the exogenous signaling molecules, such as salicylic acid (SA) and hydrogen peroxide ($H_2O_2C$), as well as pathogen attacks. These results indicate that the GhMAPK, which has a high degree of identity with group-C plant MAPKs, may also play an important role in response to environmental stresses.

Distribution characteristics and processes along flow paths of shallow groundwater in the Tan-Lu fault zone in Anhui province, China

Dejian Wang,Jiazhong Qian,Weidong Zhao,Xiaoliang Hou,Wei Wang,Lei Ma 한국지질과학협의회 2022 Geosciences Journal Vol.26 No.4

To disclose the distribution characteristics, the situation of flow and storage, and processes along flow paths of shallow groundwater in Tan-Lu fault zones, nine hundred and seven groundwater table elevations data and one hundred hydrochemical samples of shallow groundwater were taken from the Tan-Lu fault zone in Anhui province to analyze the characteristic of groundwater distribution. The geographic information system (GIS) method was used to analyze the spatial distribution characteristics of groundwater tables, total dissolved solids (TDS) and chloride ion (Cl−). Geophysical prospecting, drilling material and regional hydrogeological survey were utilized to disclose groundwater storage and flow regime in the fault zone. The results show that the Tan-Lu fault zone in Anhui province has controlled groundwater flow into the Jiashan basin, Hefei basin, Chaohu area and Qianshan basin, which developed from north to south in this area. Groundwater in theses basins have recharged from surrounding areas to form a water storage space. Geophysical prospecting and drilling technology revealed that the Tan-Lu fault zone provided a flow channel and storage space for groundwater. Faults provide preferential channels in some areas for the groundwater flow and circulation, eventually deep hot-water flows upward and discharges in the form of hot-springs. The identification of the groundwater flow pathway can help to provide a reliable scientific basis for regional spatial development and utilization of groundwater resources.

Behavior of phosphatidylcholine adsorption on CNx coated PTFE films

Wang Yang,F.Z. Cui,Xiaoliang Qing 한국물리학회 2006 Current Applied Physics Vol.6 No.5

In order to expand the applicability of PTFE for articial replacement, the CNx coatings which could inuence lipid adsorptionare of considerable relevance. The intention of this study is to gain rst information about the inuence of CNx coating on the phos-phatidylcholine (PC) adsorption of PTFE. By means of ion beam assisted deposition (IBAD), the CNx lms can have a stronglybond to the PTFE substrates as a wide atomic intermixed zone that is formed at the surface. The characterization of the CN struc-tures under dierent bombarding conditions was carried out by Raman and SEM as well as XPS measurements. For characterizingthe behavior, analysis of PC adsorption was illuminated where Fourier transform infrared spectroscopy of attenuated total internalreection mode (ATR-FTIR) was used as principal instrument. Results point to an increase of nitrogen content and sp2/sp3 fractionunder higher bombarding voltage which seems to play a dominant role for the biological characters and a dramatic restraining oflipid deposition was induced on CNx coated PTFE compared with the uncoated samples.

LSTM Android Malicious Behavior Analysis Based on Feature Weighting

( Qing Yang ),( Xiaoliang Wang ),( Jing Zheng ),( Wenqi Ge ),( Ming Bai ),( Frank Jiang ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.6

With the rapid development of mobile Internet, smart phones have been widely popularized, among which Android platform dominates. Due to it is open source, malware on the Android platform is rampant. In order to improve the efficiency of malware detection, this paper proposes deep learning Android malicious detection system based on behavior features. First of all, the detection system adopts the static analysis method to extract different types of behavior features from Android applications, and extract sensitive behavior features through Term frequency-inverse Document Frequency algorithm for each extracted behavior feature to construct detection features through unified abstract expression. Secondly, Long Short-Term Memory neural network model is established to select and learn from the extracted attributes and the learned attributes are used to detect Android malicious applications, Analysis and further optimization of the application behavior parameters, so as to build a deep learning Android malicious detection method based on feature analysis. We use different types of features to evaluate our method and compare it with various machine learning-based methods. Study shows that it outperforms most existing machine learning based approaches and detects 95.31% of the malware.

Growth and Development of Infants with Asymptomatic Congenital Cytomegalovirus Infection

Ruobing Shan,Xiaoliang Wang,Ping Fu 연세대학교의과대학 2009 Yonsei medical journal Vol.50 No.5

Purpose: To observe changes in audiology, intellectual development, behavior development, and physical growth during systematic follow-up of infants with asymptomatic congenital human cytomegalovirus (HCMV) infection. Materials and Methods: Fifty-two infants diagnosed with asymptomatic congenital HCMV infection from July 2003 to July 2007 served as the infection group, and 21 healthy infants served as the control group. All infants were confirmed to have HCMV infection by Fluorescent Quantative polymerase chain reaction (FQ-PCR). In both the infection and control groups, the neonates and infants at 3 months, 6 months, and 1 year of age underwent examinations. Results: 1) 20 items of National Black Nurses Association (NBNA) scores of neonates 12-14 days after birth in 2 groups were 38.3 ± 1.95 and 38.5 ± 2.29, without significant differences. 2) Auditory test: 50 ears of 25 cases in the infection group showed abnormal auditory thresholds in V waves with an abnormal rate of 14%, while no abnormalities were found in 21 cases in the control group. 3) Mental and psychomotor development index scores in the control group (107.49 ± 11.31 and 107.19 ± 10.98) were compared with those in 41 asymptomatically infected infants at 1 year of age (107.21 ± 9.96 and 108.31 ± 11.25), and no statistically significant difference was noted. Conclusion: 1) An elevated threshold in the V wave was present in asymptomatically infected infants, but could not be detected through otoacoustic emission (OAE) screening. 2) Either in the neonatal or infant periods, asymptomatic congenital HCMV infection did not have a significant influence on nervous behavior or on physical and intellectual development. Purpose: To observe changes in audiology, intellectual development, behavior development, and physical growth during systematic follow-up of infants with asymptomatic congenital human cytomegalovirus (HCMV) infection. Materials and Methods: Fifty-two infants diagnosed with asymptomatic congenital HCMV infection from July 2003 to July 2007 served as the infection group, and 21 healthy infants served as the control group. All infants were confirmed to have HCMV infection by Fluorescent Quantative polymerase chain reaction (FQ-PCR). In both the infection and control groups, the neonates and infants at 3 months, 6 months, and 1 year of age underwent examinations. Results: 1) 20 items of National Black Nurses Association (NBNA) scores of neonates 12-14 days after birth in 2 groups were 38.3 ± 1.95 and 38.5 ± 2.29, without significant differences. 2) Auditory test: 50 ears of 25 cases in the infection group showed abnormal auditory thresholds in V waves with an abnormal rate of 14%, while no abnormalities were found in 21 cases in the control group. 3) Mental and psychomotor development index scores in the control group (107.49 ± 11.31 and 107.19 ± 10.98) were compared with those in 41 asymptomatically infected infants at 1 year of age (107.21 ± 9.96 and 108.31 ± 11.25), and no statistically significant difference was noted. Conclusion: 1) An elevated threshold in the V wave was present in asymptomatically infected infants, but could not be detected through otoacoustic emission (OAE) screening. 2) Either in the neonatal or infant periods, asymptomatic congenital HCMV infection did not have a significant influence on nervous behavior or on physical and intellectual development.

Comparison of fractal properties of porous structures during coal devolatilization

Rong He,Xiaoliang Wang 한국화학공학회 2007 Korean Journal of Chemical Engineering Vol.24 No.3

to char pores, coal pores can also be classified as micro pores and macro pores based on their fractal dimensions. Thespecific surface area and fractal dimension of micro pores in coal particles are basicaly unchanged after devolatiliza-tion. However, the specific surface area and fractal dimension of macro pores, which are key factors in char combustion,are increased after devolatilization. In fact, the fractal dimensions are basically doubled. These parameters will affectanother fractal geometrical factor β in char pores that is correlated to char combustion rate. Since the rate of char com-the properties of their parent coal pores in the future.

Preparation of Nano-Convex and Nano-Concave-Patterned Polyimide Surfaces and their Nano-Tribological Behavior

Chunxia Wu,XIAOLIANG ZHANG,HONGWEI CHE,JINGBO MU,Guangshuo Wang,Zhixiao Zhang,Guohua Cui 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.4

In this work, nano-convex-patterned polyimide surface (notated as 1-sample) and nano-concavepatterned polyimide surface (notated as 2-sample) were prepared by self-assembly and etching. Atomic force microscope (AFM) with a colloidal probe was used to examine the adhesion and nano-tribological behavior of the 1-sample and 2-sample. Results suggest that the 1-sample and 2-sample can decrease the surface friction and adhesive forces because of the decreased contact area between the contacting pairs. The friction forces of the 1-sample and 2-sample increased with the increase in sliding velocity and applied load. Moreover, the nano-concave pattern is more effective in reducing the adhesive force than the nano-convex pattern because of its higher surface roughness. However, the nano-convex patterning is more effective in reducing the friction force than the nano-concave patterning because of the smaller area of contact between the 2-sample and the colloidal probe.

Strength and Mechanism of Carbonated Solidified Clay with Steel Slag Curing Agent

Man Li,Qiang Wang,Jingdong Yang,Xiaoliang Guo,Wenjun Zhou 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.3

Industrial wastes, such as steel slag and desulfurized gypsum, are piled up in large quantities and only a very small portion is recycled, adversely impacting natural ecosystems. Meanwhile, environmental problems caused by CO2 have increasingly received attention. Hence, this study introduces a novel environmentally friendly composite, formed by sodium hydroxide (used as an activator), desulfurized gypsum and steel slag (S-GS). The main objective of this study is to evaluate the potential use of S-GS for solidifying clay under the condition of carbonation curing. Besides, the samples’ characteristics are investigated according to the tests of unconfined compressive strength (UCS), pH, carbonation depth, quality loss and scanning electron microscope (SEM). It is found that the UCS of solidified clay decreases with the increasing water content while it increases first and then decreases with the increasing desulfurized gypsum content, reaching the maximum when the water content is 0.5 times the liquid limit and the content of desulfurized gypsum is 8%. Moreover, compared with the standard curing, carbonation curing can stimulate the activity of S-GS to improve the UCS of samples more effectively. With the increasing carbonation curing time, the mass loss rate and carbonation depth of samples increase while the pH value decreases. Additionally, based on the normalized analysis, the carbonation time has the most significant effect on the UCS. Furthermore, the SEM results indicate that formation of Calcium carbonate and Magnesium carbonate are primary reasons for improving the UCS of the stabilised clay during carbonization. This research promotes steel slag and desulfurized gypsum as green stabilisers for soil stabilization, and the method of carbonation curing contributes to the higher UCS, which also greatly shortens the curing time.

MiR-200c-3p inhibits LPS-induced M1 polarization of BV2 cells by targeting RIP2

Zhao Lei,Liu Xiaosong,Yang Jiankai,Wang Xiaoliang,Liu Xiaomeng,Wu Jianliang,Li Chen,Xu Donggang,Hu Yuhua 한국유전학회 2022 Genes & Genomics Vol.44 No.4

Background: Microglia are important immune cells, which can be induced by lipopolysaccharide (LPS) into M1 phenotype that express pro-inflammatory cytokines. Some studies have shown that microRNAs play critical roles in microglial activation. Objective: This study was designed to investigate the role of miR-200c-3p in regulating inflammatory responses of LPS-treated BV2 cells. Methods: The expression of miR-200c-3p in BV2 cells was detected by real-time PCR. Receptor-interacting protein 2 (RIP2) was predicted as a target gene of miR-200c-3p. Their relationship was verified by dual-luciferase reporter assay. The function of miR-200c-3p and RIP2 in microglial polarization and NF-κB signaling was further evaluated. Results: LPS treatment reduced miR-200c-3p expression in a dose-dependent and time-dependent manner in BV2 cells. LPS treatment increased the expression of M1 phenotype markers inducible nitric oxide synthase (iNOS) and major histocompatibility complex class (MHC)-II, promoted the release of pro-inflammatory cytokines interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α, and enhanced the nuclear translocation and phosphorylation of nuclear factor-kappaB (NF-κB) p65. Reversely, miR-200c-3p mimics down-regulated the levels of these inflammatory factors. Furthermore, RIP2 was identified to be a direct target of miR-200c-3p. RIP2 knockdown had a similar effect to miR-200c-3p mimics. Overexpression of RIP2 eliminated the inhibitory effect of miR-200c-3p on LPS-induced M1 polarization and NF-κB activation in BV2 cells. Conclusions: MiR-200c-3p mimics suppressed LPS-induced microglial M1 polarization and NF-κB activation by targeting RIP2. MiR-200c-3p/RIP2 might be a potential therapeutic target for the treatment of neuroinflammation-associated diseases.