MiR-150-5p Suppresses Colorectal Cancer Cell Migration and Invasion through Targeting MUC4

Wang, Wei-Hua,Chen, Jie,Zhao, Feng,Zhang, Bu-Rong,Yu, Hong-Sheng,Jin, Hai-Ying,Dai, Jin-Hua Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Growing evidence suggests that miR-150-5p has an important role in regulating genesis of various types of cancer. However, the roles and the underlying mechanisms of miR-150-5p in development of colorectal cancer (CRC) remain largely unknown. Transwell chambers were used to analyze effects on cell migration and invasion by miR-150-5p. Quantitative real-time PCR (qRT-PCR), Western blotting and dual-luciferase 3' UTR reporter assay were carried out to identify the target genes of miR-150-5p. In our research, miR-150-5p suppressed CRC cell migration and invasion, and MUC4 was identified as a direct target gene. Its effects were partly blocked by re-expression of MUC4. In conclusiomn, miR-150-5p may suppress CRC metastasis through directly targeting MUC4, highlighting its potential as a novel agent for the treatment of CRC metastasis.

Prognostic Value of Baseline and Interim Total Metabolic Tumor Volume and Total Lesion Glycolysis Measured on 18F-FDG PET-CT in Patients with Follicular Lymphoma

Jin-Hua Liang,Yun-Ping Zhang,Jun Xia,Chong-Yang Ding,Wei Wu,Li Wang,Lei Cao,Hua-Yuan Zhu,Lei Fan,Tian-Nv Li,Jian-Yong Li,Wei Xu 대한암학회 2019 Cancer Research and Treatment Vol.51 No.4

Purpose The purpose of this study was to investigate the prognostic significance of total metabolic tumor volume (TMTV) and total lesion glycolysis (TLG) in patients with follicular lymphoma (FL) at baseline and mid-treatment with 18F-fluorodeoxyglucose positron emission tomography computed tomography (PET-CT) scans. Materials and Methods The study analyzed data from 48 patients with FL who were treated in Jiangsu Province Hospital and reviewed their baseline PET-CT scans. TMTV and TLG were computed by using the absolute value of 2.0, 2.5, and 3.0 thresholding method, respectively. Results Median age was 53 years, 75.0% of patients had stage III to IV disease, 43.8% had a Follicular Lymphoma International Prognostic Index 1 (FLIPI1) score of 3 to 5 and 20.8% had a FLIPI2 score of 3 to 5. Receiver operating characteristic (ROC) curve analysis showed the optimal cut-off values for TMTV3.0 and TLG3.0 were 476.4 (sensitivity, 85.7%; specificity, 78.0%; area under the curve [AUC], 0.760; p=0.003) and 2,676.9 (sensitivity, 71.4%; specificity, 78.0%; AUC, 0.760; p=0.003). On multivariable analysis, TMTV3.0 and TLG3.0 were independent predictors of both progression-free survival (PFS) (hazard ratio [HR], 5.406; 95% confidence interval [CI], 1.326 to 22.040; p=0.019 and HR, 6.502; 95% CI, 1.079 to 39.182; p=0.042) and overall survival (OS) (HR, 4.111; 95% CI, 1.125 to 15.027; p=0.033 and HR, 5.885; 95% CI, 1.014 to 34.148; p=0.049). ROC curve analysis showed the optimal cut-off values for TMTV3.0 and TLG3.0 were 66.3% (sensitivity, 85.7%; specificity, 63.4%; AUC, 0.774; p < 0.001) and 64.5% (sensitivity, 85.7%; specificity, 65.9%; AUC, 0.777; p < 0.001). Conclusion Baseline TMTV and TLG are strong predictors of PFS and OS in FL. Furthermore, interim TMTV (TMTV > 66.3%) and TLG (TLG > 64.5%) reduction are valuable tools for early treatment response assessment in FL patients.

New Safrole Oxide Derivatives: Synthesis and in vitro Antiproliferative Activities on A549 Human Lung Cancer Cells

Wang, Li-Ying,Wang, Xiu-Hua,Tan, Jia-Lian,Xia, Shuai,Sun, Heng-Zhi,Shi, Jin-Wen,Jiang, Ming-Dong,Fang, Liang,Zuo, Hua,Dupati, Gautam,Jang, Kiwan,Shin, Dong-Soo Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.11

A number of novel small molecules, safrole oxide derivatives 4a-c, 6a-c, 9a-h, were synthesized by the reaction of safrole oxide with anilines 3 and 5, or its alkyl allyl ether derivative 7 with alkyl bromide 8 in moderate yields. The antiproliferative effects of all the target molecules on A549 cell growth were investigated and it was found that the 14 novel compounds could suppress A549 lung cancer cell growth. Among them, compound 6b was the most effective compound in inhibiting the proliferation of A549 cells.

The Prognostic Role of Circulating Epstein-Barr Virus DNA Copy Number in Angioimmunoblastic T-Cell Lymphoma Treated with Dose-Adjusted EPOCH

Jin-Hua Liang,Luo Lu,Hua-Yuan Zhu,Wang Li,Lei Fan,Jian-Yong Li,Wei Xu 대한암학회 2019 Cancer Research and Treatment Vol.51 No.1

Purpose Determine the frequency and prognostic value of circulating Epstein-Barr virus (EBV) DNA copy number in angioimmunoblastic T-cell lymphoma (AITL) patients who were treated with dose-adjusted etoposide, prednisone, vincristine, cyclophosphamide and doxorubicin (DA-EPOCH) regimens. Materials and Methods Sixty newly-diagnosed AITL patients were retrospectively enrolled in the present study. All patients were treated with DA-EPOCH regimen. Results Twenty-two subjects (36.7%) had a EBV DNA-positive test at diagnosis. EBV DNApositive patients were associated with lower lymphocyte-monocyte ratio (p=0.024). Median followup was 40 months (range, 14 to 100 months). The overall response rate for all the 60 AITL patents were 71.7% (95% confidence interval [CI], 58.6 to 82.5) with 3-year progressivefree survival (PFS) rate of 30.9%±6.1% and overall survival (OS) rate of 60.1%±6.6%. Not only did PFS estimation differ between the EBV DNApositive and EBV DNAnegative group (hazard ratio [HR], 2.24; 95% CI, 1.15 to 4.35; p=0.006), but also worse OS was observed in the pretreatment EBV DNApositive group than in the EBV DNAnegative group (HR, 2.74; 95% CI, 1.22 to 6.19; p=0.006). EBV DNA test positivity was independent prognostic marker for both PFS (HR, 2.17; 95% CI, 1.17 to 4.00; p=0.014) and OS (HR, 3.24; 95% CI, 1.48 to 7.11; p=0.004) after adjusting International Prognostic Index and prognostic index for AITL score. Reduction in EBV copies was significantly associated with therapy-response. Conclusion Circulating EBV DNA level was an important prognostic and monitoring marker for AITL patients who treated with DA-EPOCH regimens which cannot improve outcomes for AITL patients.

Identification and Characterization of the Antifungal Substances of a Novel Streptomyces cavourensis NA4s

( Hua Qi Pan ),( Su Ya Yu ),( Chun Feng Song ),( Nan Wang ),( Hui Ming Hua ),( Jiang Chun Hu ),( Shu Jin Wang ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.3

A new actinomycete strain NA4 was isolated from a deep-sea sediment collected from the South China Sea and showed promising antifungal activities against soilborne fungal pathogens. It was identified as Streptomyces cavourensis by morphological, physiological, and phylogenetic analyses based on its 16S rRNA gene sequence. The main antifungal components were isolated and identified from the fermentation culture as bafilomycins B1 and C1. These compounds exhibited significant antifungal activities and a broad antifungal spectrum. The results suggest that the Streptomyces cavourensis NA4 and bafilomycins B1 and C1 could be used as potential biocontrol agents for soilborne fungal diseases of plants.

Heavy concrete shielding properties for carbon therapy

Jin-Long Wang,Jiade J Lu,Da-Jun Ding,Wen-Hua Jiang,Ya-Dong Li,Rui Qiu,Hui Zhang,Xiao-Zhong Wang,Huo-Sheng Ruan,Yan-Bing Teng,Xiao-Guang Wu,Yun Zheng,Zi-Hao Zhao,Kai-Zhong Liao,Huan-Cheng Mai,Xiao-Dong Korean Nuclear Society 2023 Nuclear Engineering and Technology Vol.55 No.6

As medical facilities are usually built at urban areas, special concrete aggregates and evaluation methods are needed to optimize the design of concrete walls by balancing density, thickness, material composition, cost, and other factors. Carbon treatment rooms require a high radiation shielding requirement, as the neutron yield from carbon therapy is much higher than the neutron yield of protons. In this case study, the maximum carbon energy is 430 MeV/u and the maximum current is 0.27 nA from a hybrid particle therapy system. Hospital or facility construction should consider this requirement to design a special heavy concrete. In this work, magnetite is adopted as the major aggregate. Density is determined mainly by the major aggregate content of magnetite, and a heavy concrete test block was constructed for structural tests. The compressive strength is 35.7 MPa. The density ranges from 3.65 g/cm³ to 4.14 g/cm³, and the iron mass content ranges from 53.78% to 60.38% from the 12 cored sample measurements. It was found that there is a linear relationship between density and iron content, and mixing impurities should be the major reason leading to the nonuniform element and density distribution. The effect of this nonuniformity on radiation shielding properties for a carbon treatment room is investigated by three groups of Monte Carlo simulations. Higher density dominates to reduce shielding thickness. However, a higher content of high-Z elements will weaken the shielding strength, especially at a lower dose rate threshold and vice versa. The weakened side effect of a high iron content on the shielding property is obvious at 2.5 µSv=h. Therefore, we should not blindly pursue high Z content in engineering. If the thickness is constrained to 2 m, then the density can be reduced to 3.3 g/cm³, which will save cost by reducing the magnetite composition with 50.44% iron content. If a higher density of 3.9 g/cm³ with 57.65% iron content is selected for construction, then the thickness of the wall can be reduced to 174.2 cm, which will save space for equipment installation.

Halomonas alkalitolerans sp. nov., a Novel Moderately Halophilic Bacterium Isolated from Soda Meadow Saline Soil in Daqing, China

Shuang Wang,Qian Yang,Zhi-Hua Liu,Lei Sun,Dan Wei,Jun-Zheng Zhang,Jin-Zhu Song,Yun Wang,Jia Song,Jin-Xia Fan,Xian-Xin Meng,Wei Zhang 한국미생물학회 2011 The journal of microbiology Vol.49 No.1

A moderately halophilic bacterial strain 15-13^T, which was isolated from soda meadow saline soil in Daqing City, Heilongjiang Province, China, was subjected to a polyphasic taxonomic study. The cells of strain 15-13^T were found to be Gram-negative, rod-shaped, and motile. The required growth conditions for strain 15-13^T were: 1-23% NaCl (optimum, 7%), 10-50°C (optimum, 35°C), and pH 7.0-11.0 (optimum, pH 9.5). The predominant cellular fatty acids were C18:1 ω7c (60.48%) and C16:0 (13.96%). The DNA G+C content was 67.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain 15-13^T clustered within a branch comprising species of the genus Halomonas. The closest phylogenetic neighbor of strain 15-13^T was Halomonas pantelleriensis DSM 9661^T (98.9% 16S rRNA gene sequence similarity). The level of DNA-DNA relatedness between the novel isolated strain and H. pantelleriensis DSM 9661^T was 33.8%. On the basis of the phenotypic and phylogenetic data, strain 15-13^T represents a novel species of the genus Halomonas, for which the name Halomonas alkalitolerans sp. nov. is proposed. The type strain for this novel species is 15-13^T (=CGMCC 1.9129^T =NBRC 106539^T).

강섬유 보강된 철근콘크리트 보의 휨 강도

김애화(Jin, Ai Hua),왕기(Wang, Qi),정우진(Jeong, Woo Jin),김선우(Kim, Sun Woo),박완신(Park, Wan Shin),윤현도(Yun, Hyun Do) 한국콘크리트학회 2022 한국콘크리트학회 학술대회 논문집 Vol.34 No.2

섬유형상에 따른 모르타르에 매입된 강섬유의 인발거동

김애화(Jin, Ai Hua),왕기(Wang, Qi),정우진(Jeong, Woo Jin),김선우(Kim, Sun Woo),박완신(Park, Wan Shin),윤현도(Yun, Hyun Do) 한국콘크리트학회 2022 한국콘크리트학회 학술대회 논문집 Vol.34 No.1

宋代的蔬菜與蔬菜業

張金花(Zhang Jin-hua),王茂華(Wang Mao-hua) 한국외국어대학교 역사문화연구소 2009 역사문화연구 Vol.33 No.-

Based on previous, this article tries doing a systematic analysis of Sung vegetables and vegetable industry. There were about forty to fifty different types of vegetables in Sung, The whole vegetable category was similar to contemporary, and cultivation, storage technology was mature. The Sungs recognized that vegetables of different varieties have different sowing season, different varieties of vegetables and loose soil tilth required different degrees. In dishes with cultivation methods, all regions have geographical differences in common. There is a growing vegetables in the water garden of turnip in South, which popularized in Guangdong, Zhejiang and Sichuan. North was also about governance intensive vegetable bed. The Sungs mastered a seed treatment, germination, seed and nursery technology, in particular, pregermination way the use of incubation, the use of sulfur and other minerals to increase vegetable production, to improve quality and sealed storage of vegetables. People exclaimed their sophisticated skills. Along with the improvement of productivity, urbanization, by the former generation of self sufficiency, Sung’s Vegetable industry was tended to specialization and commercialization. Both the southern border areas or rural and urban, there were extensive gardens and vegetable production base. Vegetables should be on consumer demand in the city, the out skirts of the city was the emergence of specialized vegetable production base. Because of the relations of production of vegetables, people's livelihood, huge demand, Lucrative, low risk, it attracted all classes. In addition to the vegetable gardens of the ordinary farmers and citizens, there were vegetable gardens owed officials, government run and operated by the monastery, which had amazing size. At the same time, Sung government was also aware of the profit, gradually strengthened the management and revenue of vegetable industry. 본 論文은 이전 연구 성과를 토대로 宋代의 蔬菜와 蔬菜業에 대하여 일련의 분석과 연구를 하고자 한다. 宋代 蔬菜의 種類는 約 四五十種이고 大體的으로 當代의 蔬菜品類는 매우 다양하였다. 재배와 儲藏 技術이 상당히 발달하였다. 宋代의 사람들은 각각의 蔬菜品種별로 그 파종 시기, 土質과 疏鬆度(토질이 푸석푸석한정도)가 다르다는 것을 알고 있었다. 菜品과 재배 方法上에 地區間에 共性과 差異가 있으며 南方에서는 水面에 蔬菜를 재배하는 葑園이 이었으며 兩廣, 兩浙, 蜀等地에서 보편화 되었다. 北方에서는 精耕细作을 중시하였다. 宋人은 種子處理, 催芽, 播種과 育苗技術을 알고 있었으며 특히 孵化方式을 이용하여 催芽를 하였으며 硫磺等의 礦物質을 이용하여 蔬菜의 產量과 品質을 향상 시켰고 蔬菜를 密封储藏技術等을 사용하였다. 生產力의 향상과 城市化等으로 인하여 宋代의 蔬菜業은 前代의 自給自足인 형태에서 專業化와 商品化로 변화하였다. 江南, 邊境地區의 農村과 城鎮을 막론하고 菜園과 蔬菜生產基地가 광범히 존재하였다. 城市 에서의 蔬菜의 消費需求로 인하여 近郊에 城市에 專門面的으로 蔬菜를 공급하는 蔬菜生產基地가 생겨나게 되었다. 蔬菜의 生產은 民生과 關係되었고, 需求가 龐大하고 利潤이 豐厚하였으며 風險이 비교적 적은 이유로 社會各階層이 蔬菜業에 종사하게 하였다. 普通의 農戶와 市民經營的 菜圃 이외에 士大夫 혹은 官員, 官營, 寺院等에서 菜圃를 經營하였으며 規模또한 매우 컸다. 同時에 宋政府 또한 蔬菜業에서 많은 利益이 발생하는 것을 인식하고 逐漸적으로 蔬菜业에 대한 管 理와 稅收를 강화하게 되었다.