A nonNMDA antagonist, GYKI 52466 improves microscopic O₂balance in the cortex during focal cerebral ischemia

Chi, Oak Z.,Chang, Qiang,Wang, Guolin,Liu, Xia,Harvey R. Weiss 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Oak Z.Chi,Qiang Chang, Guolin Wang*, Xia Liu, Harvey R. Weiss□.Deprtments of Anesthesai, Departments of Physiology and Biophysics, University of Medicne and Dentisrty of New Jersey,Robert Wood Johnson Medical School, New Brunswick, New Jersey, USA and*Department of Anesthesia, Medical University, Tianjing, People's Republic of China. A non-NMDA antagonist, GYKI 52466 improves microscopic O² balance in the cortex during focal cerebral ischemia. Proceedings of International Symposium on East-West Medicine, Seoul. 172-182, 1999.-This study was performed to test whether GYKI 52466, a non-NMDA receptor antagonist, would improve microregional oxygen supply and consumption balance in the focal cerebral ischemic area. Rats were anesthetized with 1.4% isoflurance. For the GYKI Group (n=8), 19 min before middle cerebral artery (MCA) occlusion, a bolus of 5mg/kg of GYKI 52466 iv was administered and was followed by an infusion of 5mg/kg/hr. For the control Group(n=8), the same volume of the vehicle was administered. One hour after MCA occlusion, regional cerebral blood flow (rCBF) was measured using the 14C-iodoantipyrine autoradiographic technique. Microscopic arterial and venous oxygen saturations were determined using microspectrophotometry. In the cortex contralateral to MCA occlusion, the average rCBF and the average O² consumption were lower in the GYKI Group than in the Control Group (rCBF:GYKI 65.5±24.1, Control 97.7 33.4ml/100g/min;O² consumption: GYKI3.9±1.2, Control 6.2±2.5ml O²/100g/min) without a significant difference in the number of veins with SvO²<50%. In the ischemic cortex, the number of veins with SvO²<50% was significantly smaller in the GYKI Group (21 veins out of 63)than in the Control Group(45 out of 59)without a significant difference in the average rCBF(GYKI44.9±17.7, Control 29.7±10.4) or regional O² consumption between these two groups (GYKI 3.3±1.4,Control 27.7±1.2). Our data demonstrated that GYKI 52466 was effective in improving microscopic O² balance in the focal ischemic cortical area of the brain and it decreased O² consumption in the non-ischemic cortex. [Neurological Research 1999;21:299-304]

Influence of Contact Area Deformation Distribution of Tire on Tire Noise, Rolling Resistance and Dry Grip Performance

Guolin Wang,Kangying Yu,Chen Liang,Ye Mei 한국자동차공학회 2021 International journal of automotive technology Vol.22 No.1

Most tire properties are closely related to the pattern deformation. In order to explore the relationship between tire performance and defined deformation parameters in the contact area between the pneumatic tire and road surface, ten types of 205/55R16 passenger car radial (PCR) tires were selected as the research objects. Using VIC-3D non-contact strain measurement system and by subdividing the contact area, the pattern deformation in contact area under vertical load was tested, and the relevant deformation parameters of the contact area were extracted. Correlation analysis was used to establish the relationship between the identified deformation parameters and tire performance indicators. Then an evaluation method of tire performance based on the deformation parameters was proposed, as well as clarification of the contradictory mechanism between tire noise, rolling resistance and dry grip performance. While the results indicate tire noise having a significant negative correlation with radial deformation in the contact area, rolling resistance coefficient gave a significant positive correlation with its equivalent strain in the XY plane of the contact area, and dry braking distance positively correlates with tensile strain in the Y direction of contact area and negatively correlates with tensile strain in the X direction of the contact area.

NUMMERICAL SIMULATION-BASED ANALYSIS OF THE EFFECT OF CURING BLADDERS ON SHAPING OF TIRES

Wang Guolin,Zhao Wei,Zhou Haichao,Liang Chen 한국자동차공학회 2023 International journal of automotive technology Vol.24 No.4

The tire building process is a key part of tire manufacturing, serving as a bridge between the construction design and the finished tire, and the bladder is the core component to complete the shaping process. With the help of finite element method, the tire building process can be effectively reproduced, which help to carry out targeted problem solving and solution design. In this paper, the numerical simulation method is used to study the 205/55R16 radial tire building process, and the reliability of the simulation method is verified by comparing simulated green tire and its test section; then the shaping simulation model incorporating the bladder is established and compared with the finished tire parts to illustrate the reliability; On this basis, the influence of bladder parameters was analyzed by orthogonal design of experiment and simulated annealing optimization algorithm, the sensitivity of different parameters was obtained, the bladder parameters were optimized; compared with the bladder with original parameters, the optimized bladder effectively reduced the stress by 44.67 % and 55.54 % , while significantly improving the contact force between the bladders and the green tire. The results of the study provide a good methodological basis and theoretical guidance for tire design, manufacturing and bladder optimization.

Application of Variable Projection Method based on Gram-Schmidt Orthogonalization in Spatial Cartesian Coordinate Transformation Model

Luyao Wang,Guolin Liu,Qiuxiang Tao,Ke Wang 대한토목학회 2019 KSCE Journal of Civil Engineering Vol.23 No.12

For the linear and nonlinear parameters that can be separated in the spatial Cartesian coordinate transformation model, we use the variable projection algorithm in this paper to represent the linear parameters with nonlinear parameters, which are transformed into least squares problems with only nonlinear parameters. We simplify the matrix of the nonlinear function by the Gram-Schmidt orthogonalization method, and combine the nonlinear least squares iterative method with the Levenberg-Marquardt (LM) algorithm to solve for the coordinate transformation parameters. Experiments are carried out by solving for the coordinate transformation parameters of the independent spatial Cartesian coordinate system and the CGCS2000 coordinate system. We compare the solution results of the four methods (parameter non-separation method, traditional variable projection method, variable projection method based on QR decomposition, and variable projection method based on Gram-Schmidt orthogonal decomposition) with respect to the calculated results, the number of iterations and the computation time. The experimental results show that the proposed method in this paper requires a lower computation time and achieves higher computational efficiency when obtaining the same solution results and with the same number of iterations.

Two new triterpenoid saponins derived from the leaves of Panax ginseng and their antiinflammatory activity

Li, Fu,Cao, Yufeng,Luo, Yanyan,Liu, Tingwu,Yan, Guilong,Chen, Liang,Ji, Lilian,Wang, Lun,Chen, Bin,Yaseen, Aftab,Khan, Ashfaq A.,Zhang, Guolin,Jiang, Yunyao,Liu, Jianxun,Wang, Gongcheng,Wang, Ming-Kui The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.4

Background: The leaves and roots of Panax ginseng are rich in ginsenosides. However, the chemical compositions of the leaves and roots of P. ginseng differ, resulting in different medicinal functions. In recent years, the aerial parts of members of the Panax genus have received great attention from natural product chemists as producers of bioactive ginsenosides. The aim of this study was the isolation and structural elucidation of novel, minor ginsenosides in the leaves of P. ginseng and evaluation of their antiinflammatory activity in vitro. Methods: Various chromatographic techniques were applied to obtain pure individual compounds, and their structures were determined by nuclear magnetic resonance and high-resolution mass spectrometry, as well as chemical methods. The antiinflammatory effect of the new compounds was evaluated on lipopolysaccharide-stimulated RAW 264.7 cells. Results and conclusions: Two novel, minor triterpenoid saponins, ginsenoside $LS_1$ (1) and 5,6-didehydroginsenoside $Rg_3$ (2), were isolated from the leaves of P. ginseng. The isolated compounds 1 and 2 were assayed for their inhibitory effect on nitric oxide production in LPS-stimulated RAW 264.7 cells, and Compound 2 showed a significant inhibitory effect with $IC_{50}$ of $37.38{\mu}M$ compared with that of NG-monomethyl-L-arginine ($IC_{50}=90.76{\mu}M$). Moreover, Compound 2 significantly decreased secretion of cytokines such as prostaglandin $E_2$ and tumor necrosis factor-${\alpha}$. In addition, Compound 2 significantly suppressed protein expression of inducible nitric oxide synthase and cyclooxygenase-2. These results suggested that Compound 2 could be used as a valuable candidate for medicinal use or functional food, and the mechanism is warranted for further exploration.

An effective process for removing organic compounds from oily sludge using soluble metallic salt

Guolin Jing,Mingming Luan,Chunjie Han,Tingting Chen,Huaiyuan Wang 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.4

This study evaluates the application of wet oxidation for the treatment of oily sludge by using hydrogen peroxide as oxidant instead of air at high temperature in a batch reactor. The experimental results show that the quantity of oxidant with 250% to theoretical oxygen demand of oily sludge is comparatively effective and economica1. Adding a catalyst, Fe3+, significantly improved the COD removal. Reaction mechanism for WO of oily sludge is analyzed. Oxidation of intermediate for oily sludge is a rate-limiting step for further oxidation of oily sludge.

CXCL-13 Regulates Resistance to 5-Fluorouracil in Colorectal Cancer

Guolin Zhang,Xin Luo,Wei Zhang,Engeng Chen,Jianbin Xu,Fei Wang,Gaoyang Cao,Zhenyu Ju,Dongai Jin,Xuefeng Huang,Wei Zhou,Zhangfa Song 대한암학회 2020 Cancer Research and Treatment Vol.52 No.2

Purpose 5-Fluorouracil (5-Fu) is used as a conventional chemotherapy drug in chemotherapy for patients with advanced colorectal cancer, but many patients still suffer from treatment failure due to 5-Fu resistance. Emerging observations revealed the important role of chemokine (C-X-C motif) ligand 13 (CXCL-13) in tumor microenvironment and its relationship with prognosis in patients with colorectal cancer. This study is designed to reveal the important role of CXCL-13 in causing colorectal cancer resistance to 5-Fu. Materials and Methods CXCL-13 levels of patient's serum or cell culture supernatants were measured separately by enzyme-linked immunosorbent assay. In cell assays, cell viability is detected by Cell Counting Kit-8. Therefore, the recombinant human CXCL-13 was used to simulate its high expression in cells while its antibody and siRNA were used to reduce CXCL-13 expression in cells. Results In this study, we demonstrated that CXCL-13 is associated with 5-Fu resistance by culture medium exchange experiments and cytokine arrays of colorectal cancer resistant and nonresistant cells. Clinical studies showed that CXCL-13 is highly expressed in the serum of 5-Fu–resistant patients. High levels of serum CXCL-13 also predict a worse clinical outcome. The addition of recombinant CXCL-13 cytokine resulted in 5-Fu resistance, while its antibody overcame 5-Fu resistance, and knockdown of CXCL-13 expression by siRNA also reduced 5-Fu resistance, which can be saved by added recombination CXCL-13. Conclusion These results not only identify a CXCL-13 mediated 5-Fu resistance mechanism but also provide a novel target for 5-Fu–resistant colorectal cancer in prevention and treatment strategies.

Identification of a nuclear-recessive gene locus for male sterility on A2 chromosome using the Brassica 60 K SNP array in nonheading Chinese cabbage

Guolin Zhou,Xia Li,Aihua Wang,Feng Zu,Zhenhua Hu,Jiazao Lin,Jinxing Tu 한국유전학회 2016 Genes & Genomics Vol.38 No.12

WS24-3 is a newly bred recessive genic male sterility line of the non-heading Chinese cabbage (Brassica rapa ssp. chinensis). Here, an F2 population was produced from the cross between WS24-3 and a male-fertile breeding line (WS135). The Illumina Brassica 60 K single nucleotide polymorphism (SNP) array was used for SNPs detecting between sterile and fertile bulks from the F2 population, and 62 SNPs were identified. BLAST analysis of the 62 SNPs revealed that the A2 chromosome of Brassica rapa genome contained 22 SNPs, whereas the other chromosomes did not contain more than 6 SNPs each. These data indicated that the potential target gene locus, named Bra2Ms, might be located on A2. Based on 10 of the 22 SNPs, allele-specific-polymerase chain reaction (AS-PCR) primers and single sequence repeat (SSR) primers were designed, 5 AS-PCR primers and 9 SSR primers showed difference between the bulks in electrophoretic determination. Analysis of these markers in F2 population revealed that Bra2Ms was genetically delimited to a region of 1.2 cM. We also detected two co-segregated markers SSRa2-951 and SSRa2-960 in this region. The markers identified in our study might facilitate the transfer of recessive genic male sterility alleles to other favorable genetic backgrounds. Furthermore, these markers will support a map-based clone of Bra2Ms.

Dynamic Resource Reservation for Ultra-low Latency IoT Air-Interface Slice

( Guolin Sun ),( Guohui Wang ),( Prince Clement Addo ),( Guisong Liu ),( Wei Jiang ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.7

The application of Internet of Things (IoT) in the next generation cellular networks imposes a new characteristic on the data traffic, where a massive number of small packets need to be transmitted. In addition, some emerging IoT-based emergency services require a real-time data delivery within a few milliseconds, referring to as ultra-low latency transmission. However, current techniques cannot provide such a low latency in combination with a mice-flow traffic. In this paper, we propose a dynamic resource reservation schema based on an air-interface slicing scheme in the context of a massive number of sensors with emergency flows. The proposed schema can achieve an air-interface latency of a few milliseconds by means of allowing emergency flows to be transported through a dedicated radio connection with guaranteed network resources. In order to schedule the delay-sensitive flows immediately, dynamic resource updating, silence-probability based collision avoidance, and window-based re-transmission are introduced to combine with the frame-slotted Aloha protocol. To evaluate performance of the proposed schema, a probabilistic model is provided to derive the analytical results, which are compared with the numerical results from Monte-Carlo simulations.

Energy Efficient Cell Management by Flow Scheduling in Ultra Dense Networks

( Guolin Sun ),( Prince Clement Addo ),( Guohui Wang ),( Guisong Liu ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.9

To address challenges of an unprecedented growth in mobile data traffic, the ultra-dense network deployment is a cost efficient solution to off-load the traffic over other small cells. However, the real traffic is often much lower than the peak-hour traffic and certain small cells are superfluous, which will not only introduce extra energy consumption, but also impose extra interference onto the radio environment. In this paper, an elastic energy efficient cell management scheme is proposed based on flow scheduling among multi-layer ultra-dense cells by a SDN controller. A significant power saving was achieved by a cell-level energy manager. The scheme is elastic for energy saving, adaptive to the dynamic traffic distribution in the office or campus environment. In the end, the performance is evaluated and demonstrated. The results show substantial improvements over the conventional method in terms of the number of active BSs, the handover times, and the switches of BSs.