Evaluation of Genetic Diversity in Korean Soybean Landraces by Protein Banding Patterns Using High-Throughput Screening

Vijayanand Velusamy,김동섭,Kyung Jun Lee,하보근,김진백,김상훈,안준우,강시용 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.3

The agronomic performance and storage protein patterns of 722 soybean landraces collected from eight geographically different Korean locations were investigated. The days to 50% flowering, days to maturity, and 100-seed weight ranged from 68.9 to 71.9 (d),140.1 to 146.6 (d), and 22.4 to 26.8 (g), respectively. High-throughput protein profiling electrophoresis was performed, and the banding patterns were analyzed. Among the 722 soybean landraces, lipoxygenase bands were found to be absent in 21 lines. Nei’s gene diversity (h) ranged from 0 to 0.2642, with an average value of 0.1565. The mean coefficient of gene differentiation (Gst) was 0.0944, and the estimated gene flow (Nm) in the population was 4.7971. In a correlation matrix between the agronomic traits and protein banding patterns, the acidic banding pattern was significantly associated with all the other factors. The phenotypic and genotypic differences of the collection areas were evaluated, and the excellent soybean lines with high-value proteins, including 11S globulins,or without antinutritional factors such as lipoxygenase and trypsin inhibitor were selected

Genetic Diversity of Indian Jatropha Species as Revealed by Morphological and ISSR Markers

Vijayanand V,Senthil N,Vellaikumar S,Paramathma M 한국작물학회 2009 Journal of crop science and biotechnology Vol.12 No.3

The selection of Jatropha based on morphological information and molecular markers is essential as it is more reliable and consistent. Hence, twelve Jatropha accessions from different geographical areas of India were screened for genetic diversity using 19 morphological traits and 21 ISSR primers. The analysis of morphological traits grouped the accessions into five clusters. The cluster I consisted of J. curcas (CJC 18), J. curcas (CJC 20), J. curcas (CJC 22), J. curcas (CJC21), and J. curcas (CJC 25), and contained the maximum number of accessions; clusters II and IV contained the minimum number of accessions. Among all the characters, the highest range was exhibited by plant height and the least value by the number of branches. The twenty-one ISSR primers generated 156 polymorphic alleles. The average number of ISSR alleles generated was 7.47 per primer. The ISSR primer UBC 884 was highly informative with the maximum of 12 alleles. The 12 genotypes were grouped into eight clusters. The cluster I contained the maximum number of accessions, namely J. curcas (CJC 18), J. curcas (CJC 20), J. curcas (CJC 22), J. curcas (CJC21), and J. curcas (CJC 25). The clusters II, III, IV, V, VI, VII, and VIII (J. tanjorensis, J. gossypiifolia, J. glandulifera, J. podagrica, J. ramanadensis J. villosa, and J. integerrima) contained the minimum number of accessions. Maximum diversity between J. villosa and J. integerrima was noticed and the least diversity between J. curcas (CJC21) and J. curcas (CJC 25) seen because the ISSR markers differentiated the Jatropha accession into a wide genetic diversity as compared to the morphological data. The species-specific diagnostic markers identified in the study such as 1000 bp alleles for J. glandulifera by the primer UBC 826 is suitable for discriminating species of Jatropha, and thus can be used for identifying a Jatropha species from any mixed population comprising other members of the Jatropha complex. The selection of Jatropha based on morphological information and molecular markers is essential as it is more reliable and consistent. Hence, twelve Jatropha accessions from different geographical areas of India were screened for genetic diversity using 19 morphological traits and 21 ISSR primers. The analysis of morphological traits grouped the accessions into five clusters. The cluster I consisted of J. curcas (CJC 18), J. curcas (CJC 20), J. curcas (CJC 22), J. curcas (CJC21), and J. curcas (CJC 25), and contained the maximum number of accessions; clusters II and IV contained the minimum number of accessions. Among all the characters, the highest range was exhibited by plant height and the least value by the number of branches. The twenty-one ISSR primers generated 156 polymorphic alleles. The average number of ISSR alleles generated was 7.47 per primer. The ISSR primer UBC 884 was highly informative with the maximum of 12 alleles. The 12 genotypes were grouped into eight clusters. The cluster I contained the maximum number of accessions, namely J. curcas (CJC 18), J. curcas (CJC 20), J. curcas (CJC 22), J. curcas (CJC21), and J. curcas (CJC 25). The clusters II, III, IV, V, VI, VII, and VIII (J. tanjorensis, J. gossypiifolia, J. glandulifera, J. podagrica, J. ramanadensis J. villosa, and J. integerrima) contained the minimum number of accessions. Maximum diversity between J. villosa and J. integerrima was noticed and the least diversity between J. curcas (CJC21) and J. curcas (CJC 25) seen because the ISSR markers differentiated the Jatropha accession into a wide genetic diversity as compared to the morphological data. The species-specific diagnostic markers identified in the study such as 1000 bp alleles for J. glandulifera by the primer UBC 826 is suitable for discriminating species of Jatropha, and thus can be used for identifying a Jatropha species from any mixed population comprising other members of the Jatropha complex.

Behavior of CFS built-up battened columns: Parametric study and design recommendations

Vijayanand S,Anbarasu M 국제구조공학회 2020 Structural Engineering and Mechanics, An Int'l Jou Vol.74 No.3

The structural performance of cold-formed steel (CFS) built-up battened columns were numerically investigated in this paper. The built-up column sections were formed by connecting two-lipped channels back-to-back, with a regular spacing of battens plates, and have been investigated in the current study. Finite element models were validated with the test results reported by the authors in the companion paper. Using the validated models, the parametric study was extended, covering a wider range of overall slenderness to assess the accuracy of the current design rules in predicting the design strengths of the CFS built-up battened columns. The parameters viz., overall slenderness, different geometries, plate slenderness (b/t ratio) and yield stress were considered for this study. In total, a total of 228 finite element models were analyzed and the results obtained were compared with current design strength predicted by Effective Width Method of AISI Specifications (AISI S100:2016) and European specifications (EN1993-1-3:2006). The parametric study results indicated that the current design rules are limited in predicting the accuracy of the design strengths of CFS built-up battened columns. Therefore, a design equation was proposed for the AISI and EC3 specifications to predict the reliable design strength of the CFS Built-up battened columns and was also verified by the reliability analysis.

Investigations on the Performance Characteristics of Carbon Nano-tubes, Alumina and Titanium Dioxide Based Plasma Sprayed Coatings on AISI 1020 Steel

Vijayanand Palani,Amitesh Kumar,K. R. Vijaya Kumar,P. Kumaran 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.22 No.2

The current study deals with the investigation on the performance characteristics of varying composition of coating mixture comprising carbon nano-tube, alumina and titanium dioxide on the thermal spray coating on mild steel. The performance properties, namely tensile and microhardness, were measured for the varying composition of coating mixture based thermal coating on mild steel. Response surface methodology was adopted to access the interdependencies prevailing between various dependent and independent parameters, namely composition of the mixture, tensile and microhardness. Scanning Electron Microscope coupled with elemental mapping were used to show the melting zones features and composition. The experimental results and response graphs indicated that increased weight percentages of carbon nano-tube and TiO2 with an optimal value of alumina at 87.5 percent of total weight showed better performance properties due to better dispersion leading to good bonding suitable for protective coatings. Scanning Electron Microscope images reveal partially melted zones that were restricted to smaller regions, and all particles tend to solidify beginning with a fully molten phase.

Evaluation of the Resistance of Mungbean Lines to Sprout Rot Caused by Pseudomonas species

Vijayanand Velusamy(벨루사미비제야난드),Euiho Park(박의호) 한국생명과학회 2012 생명과학회지 Vol.22 No.7

녹두나물(숙주나물)은 국내뿐만 아니라 세계적으로도 널리 이용되고 있는 채소다. 그런데 녹두나물 재배를 하는 과정에서 발생되는 녹두나물 무름병은 녹두나물 생산량은 물론 품질을 심각하게 저하시킨다. 본 연구에서는 녹두나물 부패 조직으로부터 70계통의 병원균을 분리하였으며, 각 병원균의 병원성을 검정하였다. 그 가운데 강한 병원성을 가진 Pseudomonas 균류의 계통 YV-St-033를 확인하여 선발하였으며, 분리된 병원균계의 16S rRNA 유전자 염기서열을 분석하고 유전학적 유연관계를 분석하였다. YV-St-033는 P. mosselii, P. putita, P. fluorescens, P. entomophila, P. lecoglossicida 등의 종이 속한 그룹으로 확인이 되었으며, Pseudomonas mosselii R10 strain과 가장 높은 염기서열 identity (약 99%)를 보였다. 또한 YV-St-033 strain을 이용하여 영남대학교에서 보유하고 있는 녹두 유전자원들에 대해 녹두나물 무름병 저항성을 검정하였다. 3일간 배양한 녹두에 병원균을 접종하고 녹두의 생장율을 비교한 결과 YV148 line에서 높은 저항성이 확인되었으며, 그 외 녹두 계통에서도 부분적인 저항성을 나타내었다. 숙주나물 무름병에 저항성을 보인 YV 148 계통은 나물이 가늘고 연하고 생장율이 우수하여 앞으로 품종 육종의 좋은 재료로 활용될 수 있을 것으로 판단되었다. Mungbean sprout rot is one of the most serious problems of the commercial mungbean sprout industry. In this study, 70 strains of mungbean sprout rot pathogens were isolated from rotten sprouts at different time intervals. The pathogenicity of the isolated pathogens was tested. The highly pathogenic strain (YV-St-033) was identified as Pseudomonas sp. by 16S rRNA gene sequencing. In phylogenetic analysis, the YV-St-033 strain was grouped with P. mosselii, P. putita, P. fluorescens, P. entomophila, and P. lecoglossicida. The results of the 16S rRNA gene sequence analysis revealed that the YV-St-033 strain shared the highest sequence identity (more than 99%) with the P. mosselii R10 strain. The mungbean lines of Yeungnam University germplasm were screened against the YV-St-033 strain. Based on the growth rate of the sprouts after 3 days of inoculation with the pathogen, the YV148 line was highly resistant to the pathogen. The remaining lines were either partially or fully infected. The highly resistant line YV 148 is suitable for future breeding programs due to their thin sprouts and fast growing nature.

High-throughput screening of protein variations in soybean seeds.

Vijayanand Velusamy,Kyung Jun Lee,Bo-Keun Ha,Jin Baek Kim,Sang Hoon Kim,Si-Yong Kang,Dong Sub Kim 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

The protein in soybean seeds accounts for approximately 40% of the dry seed weight. Two major storage proteins, 7S and 11S, constitute 70-80% of the total storage proteins in the seeds. In this study, the variation of total soluble protein extracts from 1152 soybean landraces that have been collected from South Korea were studied using high-throughput screening method with HT Protein Express Labchip (Caliper Life Sciences, Inc.). Seven distinct protein band patterns - four protein sub-units of 11S and three sub-units of 7S, were taken into account and their presence or absence were analyzed. Among the 1152 landraces, 525 genotypes were identified as lacking lipoxygenase, 255 lacking α1 subunit, 680 lacking α subunit, 169 lacking β subunit, 140 lacking acidic, 114 lacking Kunitz Trypsin Inhibitor (KTi) and 199 lacking basic protein patterns. The high-throughput protein analysis is helpful in screening a large number of populations with less time and minimum labor. The selected genotypes with low amounts or lacking of anti-nutritional factors such as trypsin inhibitor, lipoxygenase and α subunit would be used for future breeding purpose of quality improvement in soybean protein.

Characterizations of plasma sprayed composite coatings over 1020 mild steel

P. Vijayanand,Amitesh Kumar,K. R. Vijaya Kumar,A. Vinod,P. Kumaran,S. Arungalai Vendan 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.10

The present work deals with the coating of mild steel using plasma spray coating technique to improve surface properties for various industrial applications. Three different composite coating powders namely Al 2 O 3 :TiO 2 , Al 2 O 3 :TiO 2 : CNT and Al 2 O 3 : CNT were coated on the surface of mild steel for a thickness of 0.25 mm. Subsequently, the coated samples were tested for their mechanical characteristics namely tensile, compression and hardness followed by tribological investigations primarily comprising of wear analysis. Further, metallurgical analysis engrossing the Scanning electron microscopy, X-Ray diffraction and Energy-dispersive X-ray spectroscopy analysis were carried out to assess the homogeneity, spread uniformity and bond integrity of the developed samples. The test results revealed that the performance measure of Al 2 O 3 :TiO 2 : CNT is way ahead than other two powder coatings while carbon nanotube majorly contributes to the strength and quality of the surface coating.

Formulation and comparative pharmacokinetic evaluation of orodispersible tablets and films of nebivolol hydrochloride

P. Vijayanand,J. S. Patil,M. Venkata Reddy 한국약제학회 2015 Journal of Pharmaceutical Investigation Vol.45 No.2

Objective of the present research work was toprepare orodispersible tablets and orodispersible films ofnebivolol hydrochloride for dysphagic patients. Nebivololhydrochloride, an anti-hypertensive agent, was chosen as amodel drug in this study. Oral bioavailability of Nebivololhydrochloride is only 12 % in humans due to extensive firstpass hepatic metabolism by Cytochrome P450 2D6enzyme. Orodispersible tablets of Nebivolol hydrochloridewere prepared using different sublimating agents likecamphor, menthol and thymol. The orodispersible tabletformulation containing 10 % w/w of menthol showed adisintegration time of 11 s with more than 98 % drugrelease within 14 min. The orodispersible films of Nebivololhydrochloride were prepared using xanthan gum, guargum and sodium alginate as polymers by solvent castingmethod. Formulation containing 1.5 % w/v of sodiumalginate had disintegration time of 14 s with 99 % drugrelease within 10 min. In-vivo studies of both orodispersibletablets and orodispersible films in rabbits showedsignificantly better pharmacokinetic profile (AUC, Tmax,Cmax) compared to marketed conventional tablets of Nebivololhydrochloride. From this study, it was concludedthat both orodispersible tablets and films of Nebivololhydrochloride may prove to be more efficacious in thetreatment of hypertension in dysphagic patients.

Evaluation of Genetic Diversity in Korean Soybean Landraces by Protein Banding Patterns Using High-Throughput Screening

Velusamy, Vijayanand,Lee, Kyung Jun,Ha, Bo-Keun,Kim, Jin-Baek,Kim, Sang Hoon,Ahn, Joon-Woo,Kang, Si-Yong,Kim, Dong Sub 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.3

The agronomic performance and storage protein patterns of 722 soybean landraces collected from eight geographically different Korean locations were investigated. The days to 50% flowering, days to maturity, and 100-seed weight ranged from 68.9 to 71.9 (d), 140.1 to 146.6 (d), and 22.4 to 26.8 (g), respectively. High-throughput protein profiling electrophoresis was performed, and the banding patterns were analyzed. Among the 722 soybean landraces, lipoxygenase bands were found to be absent in 21 lines. Nei's gene diversity (h) ranged from 0 to 0.2642, with an average value of 0.1565. The mean coefficient of gene differentiation (Gst) was 0.0944, and the estimated gene flow (Nm) in the population was 4.7971. In a correlation matrix between the agronomic traits and protein banding patterns, the acidic banding pattern was significantly associated with all the other factors. The phenotypic and genotypic differences of the collection areas were evaluated, and the excellent soybean lines with high-value proteins, including 11S globulins, or without antinutritional factors such as lipoxygenase and trypsin inhibitor were selected.

Synthesis of a Novel Nano-Sized Pt/ZnO Catalyst for Water Gas Shift Reaction in Medium Temperature Application

Subramanian, Vijayanand,Potdar, Hari S.,Jeong, Dae-Woon,Shim, Jae-Oh,Jang, Won-Jun,Roh, Hyun-Seog,Jung, Un Ho,Yoon, Wang Lai Springer-Verlag 2012 Catalysis letters Vol.142 No.9