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Mathematical models applied to dispersal of pest populations in greenhouse
T. V. Nguyen,C.Xia,B.K.Chung,H.Y.Kim,T.S.Chona 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05
A genome project has been launched and aims to sequence total genome of Cotesia plutellae bracovirus. On this process, several open reading frames (ORFs) have been identified. This study was intended to clone and express protein tyrosine phosphatase genes, PTP1 and PTP6. The ORFs of these two genes consist of 900 and 891 bp, respectively. PTP1 and PTP6 are genes of a group of genes that has been implicated as important regulatory components in cell growth, differentiation and malignant transformation by certain viruses. In this work,we studied the cloning and expression patterns of these genes in Plutella xylostella, a lepidopteran host of C. plutellae. A polymerase chain reaction (PCR) produced the corresponding products of PTP1/6. These PCR products were cloned and expressed using an expression vector pBAD-TOPO, and then over-expressed using an inducer, L-arabinose. Then the purified proteins were confirmed using Western blotting (immunoblotting using V5 antibody) and the apparent molecular weights of both proteins were about 40 kDa. Expression of PTP genes were analyzed in the parasitized P. xylostella by realtime RT-PCR, which indicated late expression pattern of both PTPs during parasitization. We are pursuing to elucidate their physiological function in the parasitized host insect.
Lee, K.N.,Nguyen, T.,Kim, S.M.,Park, J.H.,Do, H.T.,Ngo, H.T.,Mai, D.T.,Lee, S.Y.,Nguyen, C.V.,Yoon, S.H.,Kweon, C.H.,Cho, I.S.,Kim, H. Elsevier Scientific Pub. Co 2011 Veterinary microbiology Vol.147 No.3
In this study, we used universal or duplex serotype-specific (O and Asia 1) RT-PCR to analyze clinical field samples of foot-and-mouth disease virus (FMDV) or virus isolates collected in Viet Nam between 2006 and 2007. We found viral serotypes O and Asia 1 circulating concurrently during this period. Direct sequencing of type-specific RT-PCR products revealed the existence of three different topotypes of serotype O: Southeast Asia (SEA), Middle East-South Asia (ME-SA), and Cathay. Of these, SEA was most prevalent during the period. All samples of serotype Asia 1 belonged to genetic group V. Based on the rooted maximum likelihood phylogenetic trees inferred from the VP1 region, new lineages in topotype SEA were originating from Viet Nam, and group V strains of Asia 1 have undergone fewer passages from the common ancestor, compared with other genetic groups. The co-circulation of different types of FMDV may complicate the individual or population genomic structures of FMDV and make conventional multiplex diagnostic methods and phylogenetic analyses with relevant evolutionary models essential in Viet Nam.
Chung, H.-C.,Nguyen, V.-G.,Oh, W.-T.,Huynh, T.-M.-L.,Moon, H.-J.,Lee, J.-H.,Kim, H.-K.,Park, S.-J.,Park, B.-K. Elsevier 2017 Transplantation proceedings Vol.49 No.9
<P><B>Abstract</B></P> <P><B>Background</B></P> <P>There might be much benefit in xenotransplantation, however, the risk of infections across species barriers remains, especially porcine endogenous retrovirus (PERV). To date, many attempts have been made to knock down active PERVs by inhibitory RNA (RNAi) and micro RNA (miRNA), which target different genes of PERV. There are a few studies that have explored whether targeting promoter regions of PERV could exert an inhibition effect.</P> <P><B>Methods</B></P> <P>miRNAs were automatically selected based on an online program BLOCK-iT RNAi Designer. The inhibition efficiency between miRNAs was compared based on their inhibition of different PERV genes: long terminal repeats (LTR), gag, and pol. Both relative quantitative real-time polymerase chain reaction (PCR) and C-type reverse transcriptase activity were performed.</P> <P><B>Results</B></P> <P>The results demonstrated that miRNA targeting the LTR region degraded the target sequence, and simultaneously inhibited the mRNA expression of both gag and pol genes of PERV. The LTR1, LTR2, and dual LTR1 + LTR2 miRNA inhibited 76.2%, 22%, and 76.8% of gag gene expression, respectively. Similarly, the miRNA was found to knock down the pol gene expression of 69.8%, 25.5%, and 77.7% for single targeting miRNA (LTR1 and LTR2) and multi-targeting miRNA (LTR1 + LTR2), respectively. A stable PK15 clone constitutively expressed dual LTR1 + LTR2 miRNA and exhibited higher inhibitory up to 82.8% and 92.7% of the expressions of the gag and pol genes, respectively. Also, the result of co-cultivation of dual LTR1 + LTR2 miRNA transfected PK15 cell with a human cell line inhibited expression of LTR, gag, and pol genes of PERV.</P> <P><B>Conclusions</B></P> <P>In conclusion, this study suggested that the LTR might be an alternative target for gene silencing of PERV, and that multi-targeting miRNA had better inhibitory effect than single- targeting miRNA. In an in vitro model, the presence of miRNA was able to reduce PERV infectivity in a human cell line.</P> <P><B>Highlights</B></P> <P> <UL> <LI> This study suggested that the LTR might be an alternative target for gene silencing of PERV, and that multi-targeting miRNA had better inhibitory effect than single- targeting miRNA. </LI> <LI> In an in vitro model, the presence of miRNA was able to reduce PERV infectivity in a human cell line. </LI> </UL> </P>
Choi, S.Y.,Nguyen, V.T.,Lee, J.c.,Kang, H.,Pandey, B.D. Elsevier Scientific Pub. Co 2014 Journal of hazardous materials Vol.278 No.-
The present paper is focused on solvent extraction of hazardous Cd(II) from acidic chloride media by Cyanex 921, a new extractant mixed with 10% (v/v) TBP in xylene. The optimum conditions for extraction and stripping of Cd(II) were investigated with an aqueous feed of 0.1mol/L Cd(II) in 2.0mol/L HCl. McCabe-Thiele diagram was in good agreement with the simulation studies, showing the quantitative extraction (99.9%) of Cd(II) within two counter-current stages utilizing 0.30mol/L Cyanex 921 at O/A ratio of 3/2 in 10min. Stoichiometry of the complexes extracted was determined and confirmed by numerical treatment and graphical method, revealing the formation of HCdCl<SUB>3</SUB>.2L and HCdCl<SUB>3</SUB>.4L for Cyanex 921(L) concentration in the range 0.03-0.1mol/L and 0.1-1.0mol/L, respectively. The thermodynamic parameters for the extraction of cadmium were also determined. The stripping efficiency of cadmium from the loaded organic with 0.10mol/L HCl was 99.6% in a three-stage counter-current process at an O/A ratio of 2/3. Cyanex 921 was successfully applied for the separation of Cd(II) from Ni(II) in the simulated leach liquor of spent Ni-Cd batteries. The study demonstrates the applicability of the present hydrometallurgical approach for the treatment of hazardous waste, the spent Ni-Cd batteries.
Nano Ni layered anode for enhanced MCFC performance at reduced operating temperature
Nguyen, H.V.P.,Othman, M.R.,Seo, D.,Yoon, S.P.,Ham, H.C.,Nam, S.W.,Han, J.,Kim, J. Pergamon Press ; Elsevier Science Ltd 2014 INTERNATIONAL JOURNAL OF HYDROGEN ENERGY - Vol.39 No.23
Nanoparticles of Ni and Ni-Al<SUB>2</SUB>O<SUB>3</SUB> were coated on a molten carbonate fuel cell (MCFC) anode by spray method to enlarge the electrochemical reaction sites at triple phase boundaries (TPBs). Both nano Ni coated anode and nano Ni-Al<SUB>2</SUB>O<SUB>3</SUB> anode exhibited significant reduction of anode polarization, thanks to smaller charge transfer resistance. The maximum power density of nano Ni coated anode was 159 mW cm<SUP>-2</SUP> at current density of 300 mA cm<SUP>-2</SUP> operating at 600 <SUP>o</SUP>C. This is about 7% increase from the standard cell performance tested and compared in the study. Although low performance of nano coated Ni-Al<SUB>2</SUB>O<SUB>3</SUB> cell is observed due to electrolyte consumption, the stability of cell performance during operation time is more favorable in MCFCs operation.
Incremental sheet forming technique and its applications
V. C. Do(도반추옹),D.T. Nguyen(규엔득투완),K. C. Park(박기철),Y. S. Kim(김영석) 한국소성가공학회 2015 금형가공 심포지엄 Vol.2015 No.8
Computer aided manufacturing technology is widely used in the sheet forming industry. Conventional press forming technique, in which the whole plate is deformed at the same time, has been the effective in mass production. To achieve the flexible manufacturing requirement, a new forming technology called incremental sheet forming (ISF) that gradually and locally deforms the sheet metal has been developed. Unlike conventional large-capacity press forming, this technology does not use the upper and lower dies. This technology has been beneficial in rapid production of prototypes and small batch production (rapid prototyping technology). The product is incrementally made by using a CNC machine or a robot that has the ability to control the path of the tool moving along the trajectory of the outer shape of the product. Nowadays, this flexible manufacturing technology has expanded its application into a variety of asymmetry or symmetry stockpile products, such as the front end of the high-speed train, aircraft shell components, dental parts etc. This study briefly summarizes the basic mechanism of the ISF technology, recent developments in ISF technology, and some applications in forming complex shaped products.