Intravenous Tenecteplase for Acute Ischemic Stroke Within 4.5–24 Hours of Onset (ROSE-TNK): A Phase 2, Randomized, Multicenter Study

Wang Lu,Dai Ying-Jie,Cui Yu,Zhang Hong,Jiang Chang-Hao,Duan Ying-Jie,Zhao Yong,Feng Ye-Fang,Geng Shi-Mei,Zhang Zai-Hui,Lu Jiang,Zhang Ping,Zhao Li-Wei,Zhao Hang,Ma Yu-Tong,Song Cheng-Guang,Zhang Yi,Ch 대한뇌졸중학회 2023 Journal of stroke Vol.25 No.3

Background and Purpose Intravenous tenecteplase (TNK) efficacy has not been well demonstrated in acute ischemic stroke (AIS) beyond 4.5 hours after onset. This study aimed to determine the effect of intravenous TNK for AIS within 4.5 to 24 hours of onset. Methods In this pilot trial, eligible AIS patients with diffusion-weighted imaging (DWI)-fluid attenuated inversion recovery (FLAIR) mismatch were randomly allocated to intravenous TNK (0.25 mg/kg) or standard care within 4.5–24 hours of onset. The primary endpoint was excellent functional outcome at 90 days (modified Rankin Scale [mRS] score of 0–1). The primary safety endpoint was symptomatic intracranial hemorrhage (sICH). Results Of the randomly assigned 80 patients, the primary endpoint occurred in 52.5% (21/40) of TNK group and 50.0% (20/40) of control group, with no significant difference (unadjusted odds ratio, 1.11; 95% confidence interval 0.46–2.66; <i>P</i>=0.82). More early neurological improvement occurred in TNK group than in control group (11 vs. 3, <i>P</i>=0.03), but no significant differences were found in other secondary endpoints, such as mRS 0–2 at 90 days, shift analysis of mRS at 90 days, and change in National Institutes of Health Stroke Scale score at 24 hours and 7 days. There were no cases of sICH in this trial; however, asymptomatic intracranial hemorrhage occurred in 3 of the 40 patients (7.5%) in the TNK group. Conclusion This phase 2, randomized, multicenter study suggests that intravenous TNK within 4.5–24 hours of onset may be safe and feasible in AIS patients with a DWI-FLAIR mismatch.

Self-assembly and Mechanism of L-Alanine-based Dihydrazide Derivative as Excellent Gelator of Organic Solvents

Wang, Chuan-Sheng,Wang, Xiao-Hong,Li, Zhi-Yuan,Wei, Wei,Shi, Zhong-Liang,Sui, Zhi-Tong Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.4

A new organogelator, L-Alanine dihydrazide derivative can self-assemble in various organic solvents and turned them into thermally reversible physical supramolecular organogels at extremely low concentrations (< 2 wt %). The gel-sol phase transition temperatures ($T_{GS}$) were determined as a function of gelator concentration and the corresponding enthalpies (${\Delta}H_g$) were extracted. Scanning electron microscopy (SEM) measurements revealed that the interspaces of fiber-like network structures were diminished with the increasing of the LMOG concentration. FT-IR spectroscopy studies revealed that hydrogen-bonding and hydrophobic interaction were the driving forces for the formation of the gels. Based on the data of XRD and molecular modeling, the possible packing modes for the formation of organogelator aggregates were proposed.

One-Pot Synthesis of CdSe Quantum Dots Using Selenium Dioxide as a Selenium Source in Aqueous Solution

Wang, Yilin,Yang, Hong,Xia, Zhenyi,Tong, Zhangfa,Zhou, Liya Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.7

A novel technology has been developed for the synthesis of thioglycolic acid (TGA)-capped CdSe quantum dots (QDs) in aqueous medium. The reaction was carried out in air atmosphere with one-pot by using $SeO_2$ to replace Se or $Na_2Se$. The technological parameters including refluxing time, pH values and molar ratios of selenium to cadmium had significant influence on the luminescence properties of CdSe QDs. Furthermore, the obtained QDs were characterized by fluorescent spectroscopy, X-ray powder diffraction (XRD) and transmission electron microscopy (TEM), respectively. The results demonstrated that the CdSe QDs were of zinc-blended crystal structure in a sphere-like shape.

Study on Drilling Ground Collapse Induced by Groundwater Flow and Prevention Based on a Coupled CFD-DEM Method

Hao-tong Zhou,Chang-qing Liu,Gui-he Wang,Kai Kang,Yu-hong Liu 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.5

Given the hazards caused by drilling ground collapse (DGC) due to groundwater flow in the processes of geotechnical engineering investigation, a coupled computational fluid dynamics and discrete element method (CFD-DEM) method is adopted to numerically simulate the development of hazards. An improved coupling program is employed to realize the two-way coupling between open-source CFD code OpenFOAM and commercial DEM software PFC3D. The coupling program allows complex meshes generated by ANSYS ICEM to be imported into PFC3D. The feasibility and accuracy of the CFD-DEM method are first verified by simulating a single spherical particle settling in hydrostatic water and the formation of the repose angle of sandpiles. Then, this method is adopted to investigate the patterns of stratum deformation and foundation loss, typical particle flow processes, and characteristics of drilling rig toppling. Finally, measures for prevention of DGC induced by groundwater flow are discussed in detail. Two typical strata, including a water-rich sand stratum and a water-rich sand-clay compound stratum, are considered. The research results show that for the water-rich sand stratum, DGC induces a “bowl”-shaped settlement trough, and the maximum value of ground surface settlement is proportional to the foundation loss rate. For the water-rich sand-clay compound stratum, a “jar”-shaped settlement trough is created. Four typical particle flow processes are involved in DGC. The drilling rig topples toward the center of the settlement trough. The maximum displacement appears at the top of the tower and has an approximate linear correlation with the groundwater flow velocity. The measures and methods of DGC prevention are also summarized in detail. The results of this research provide a reference for hazard prevention and delicacy control of DGC induced by groundwater flow.

Activated Protein C Protects Myocardium Via Activation of Anti-apoptotic Pathways of Survival in Ischemia-reperfused Rat Heart

Jia-Wang Ding,Xiao-Hong Tong,Jun Yang,Zhao-Qi Liu,Yan Zhang,Jian Yang,Song Li,Li Li 대한의학회 2010 Journal of Korean medical science Vol.25 No.11

Activated protein C (APC) is known to be beneficial on ischemia reperfusion injury in myocardium. However, the protection mechanism of APC is not fully understood. The purpose of this study was to investigate the effects and possible mechanisms of APC on myocardial ischemic damage. Artificially ventilated anaesthetized Sprague-Dawley rats were subjected to a 30 min of left anterior descending coronary artery occlusion followed by 2 hr of reperfusion. Rats were randomly divided into four groups; Sham, I/R, APC preconditioning and postconditioning group. Myocardial infarct size, apoptosis index, the phosphorylation of ERK1/2, Bcl-2, Bax and cytochrome c genes and proteins were assessed. In APC-administrated rat hearts, regardless of the timing of administration, infarct size was consistently reduced compared to ischemia/reperfusion (I/R) rats. APC improved the expression of ERK1/2 and anti-apoptotic protein Bcl-2 which were significantly reduced in the I/R rats. APC reduced the expression of pro-apoptotic genes, Bax and cytochrome c. These findings suggest that APC produces cardioprotective effect by preserving the expression of proteins and genes involved in anti-apoptotic pathways, regardless of the timing of administration.

One-Pot Synthesis of CdSe Quantum Dots Using Selenium Dioxide as a Selenium Source in Aqueous Solution

Yilin Wang,Hong Yang,Zhenyi Xia,Zhangfa Tong,Liya Zhou 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.7

A novel technology has been developed for the synthesis of thioglycolic acid (TGA)-capped CdSe quantum dots (QDs) in aqueous medium. The reaction was carried out in air atmosphere with one-pot by using SeO_2 to replace Se or Na_2Se. The technological parameters including refluxing time, pH values and molar ratios of selenium to cadmium had significant influence on the luminescence properties of CdSe QDs. Furthermore, the obtained QDs were characterized by fluorescent spectroscopy, X-ray powder diffraction (XRD) and transmission electron microscopy (TEM), respectively. The results demonstrated that the CdSe QDs were of zinc-blended crystal structure in a sphere-like shape.

A native Trichoderma harzianum strain Th62 displays antagonistic activities against phytopathogenic fungi and promotes the growth of Celosia cristata

Yue-Feng Wang,Xue-Yue Hou,Chuan-Ying Jiang,Tong-Tong Zhai,Rui Miao,Jun-Jie Deng,Zhi-Hong Yao,Rongshu Zhang 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.2

Trichoderma spp. are widely applied, eco-friendly mycofungicides and plant growth promoters. Native Trichoderma strainsare likely to have more productive, stable biocontrol and biofertilizer activities since they are well adapted to the local environment. In this study, we isolated a native Trichoderma strain ‘Th62’ from the rhizosphere soil of wild Chelidonium majusplants in Harbin, China (126.6341°E, 45.7242°N). The isolated Trichoderma strain was identifi ed as a T. harzianum strainvia morphological observation and molecular methods based on the rDNA internal transcribed spacer region and elongationfactor-1α gene sequences. Signifi cant antagonistic activities of Th62 against fi ve soil-borne fungal phytopathogens,Fusarium oxysporum , Sclerotinia sclerotiorum , Alternaria alternata , Cytospora chrysosperma, and Rhizoctonia solani ,were confi rmed by dual-culture assays. Furthermore, the crude fermentation products of Th62 also displayed antifungalactivities against these fi ve pathogens. To evaluate the function of Th62 as a biofertilizer, we subsequently applied Th62on cockscomb ( Celosia cristata L), a plant species with both ornamental and medicinal values, by inoculation with Th62conidia at diff erent concentrations, 1 × 10 10 cfu mL −1 , 1 × 10 11 cfu mL −1 , and 1 × 10 12 cfu mL −1 . The benefi cial eff ects ofTh62 were evaluated by measuring the growth and photosynthetic traits of the inoculated cockscomb plants, and the resultsdemonstrated that Th62 signifi cantly improved the photosynthetic effi ciency, photosynthetic capacity, and the adaptabilityto intense light of the inoculated cockscomb plants compared to the controls. Consistently, Th62 inoculation signifi cantlyimproved the growth and fl ower yield of cockscomb. We presented a positive case of isolating and applying native microbialresources on local plantation practices.

PREPARATION AND ANTI-CORROSION PERFORMANCE OF ZINC PHOSPHATE NANOCRYSTALS BY ULTRASONIC – HYDROTHERMAL SYNERGISTIC ROUTE

CHANG LIU,HONG-ZHEN XIE,QIN TONG,JIAN-DONG WANG,JIN-KU LIU,XIAO-HONG YANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.6

The zinc phosphate nanocrystals were synthesized by the ultrasonic – hydrothermal synergisticroute. The ultrasonic – hydrothermal synergistic route can not only decrease the size of the zincphosphate material, but also improve the crystallinity of the product. The transmission electronmicroscopy (TEM) image showed that the needle-like zinc phosphate product was 200 – 300 nmin length and 70 – 80 nm in width. The anti-corrosion tests revealed that the salt atmosphere-resistant time about 1056 h was longer than 768 h common zinc phosphate materials in themarket. The mechanisms of ultrasonic – hydrothermal synergistic route and anti-corrosion werediscussed.

Starving Aphelinus asychis negatively affects host feeding and parasitism on Myzus persicae

Sheng-Yin Wang,Yi Feng,Ning-Ning Liang,Rui Tang,Yan-Hong Liu,Da Yu Zhang,Tong-Xian Liu 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.2

Aphelinus asychis (Walker) is an effective biological control agent for many species of aphids found on various crops. To provide strategies for releasing these parasitoids into a crop ecosystem, the effects of a short-duration starvation experiment on A. asychis were measured under laboratory conditions using Myzus persicae (Sulzer) as the host on cabbage and chili pepper plants. The survival rate of starved A. asychis female adults decreased sharply as the starving time increased, and the longevity of the starved parasitoids also decreased significantly. The number of aphids killed by the parasitoids decreased gradually with the extended duration of starvation. In addition, the number of aphids on chili peppers and cabbages killed by starved A. asychis females over their lifespan was significantly lower than that in the control group. Our results indicated that a short period of starvation might not cause significantly negative effects on the parasitoids and may be beneficial for the control of M. persicae at low densities after 1 day; however, starvation for>1 day seriously affected the performance of A. asychis females over their entire lifespan. The parasitoids performed similarly when aphids were fed chili pepper or cabbage plants.

SMYD3-associated pathway is involved in the anti-tumor effects of sulforaphane on gastric carcinoma cells

Qing-Qing Dong,Qiu-Tong Wang,Lei Wang,Ya-Xin Jiang,Mei-Ling Liu,Hai-Jie Hu,Yong Liu,Hao Zhou,Hong-Peng He,Tong-Cun Zhang,Xuegang Luo 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.4

Sulforaphane (SFN), a natural compound derived from cruciferous vegetables, has been proved to possess potent anti-cancer activity. SMYD3 is a histone methyltransferase which is closely related to the proliferation and migration of cancer cells. This study showed that SFN could dose-dependently induce cell cycle arrest, stimulate apoptosis, and inhibit proliferation and migration of gastric carcinoma cells. Accompanied with these anticancer effects, SMYD3 and its downstream genes, myosin regulatory light chain 9, and cysteine-rich angiogenic inducer 61, was downregulated by SFN. Furthermore, overexpression of SMYD3 via transfection could abolish the effects of SFN, suggesting that SMYD3 might be an important mediator of SFN. To the best of our knowledge, this is the first report describing the role of SMYD3 in the anti-cancer of SFN. These findings might throw light on the development of novel anti-cancer drugs and functional food using SFN-rich cruciferous vegetables.