Combined Application of Dentin Noncollagenous Proteins and Odontogenic Biphasic Calcium Phosphate in Rabbit Maxillary Sinus Lifting

Yang Gang,Liu Xin,Huang Tianyu,Ding Ruyuan,Wang Yuanyin 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.1

BACKGROUND: Teeth can be used as a raw material for preparing bone substitutes due to their similar chemical composition to bone. The objective of our study was to evaluate the effect of odontogenic biphasic calcium phosphate (BCP) incorporating dentin noncollagenous proteins (DNCPs) on osteogenesis and stability in maxillary sinus augmentation. METHODS: The composition, structure and morphology of the odontogenic BCP were tested by X-ray powder diffraction (XRD), Brunauer–Emmett–Teller, and scanning electron microscopy methods. The biocompatibility and osteoinduction of DNCPs and materials were examined in vitro and their bone regeneration capacity was verified in vivo. RESULTS: The results showed that the cells adhered and proliferated well on the DNCP-loaded BCP scaffold. The odontogenic BCP and DNCPs promoted osteogenic differentiation of cells, The new bone formation in the BCP groups and DNCP subgroups was significantly higher than the new bone formation in the control, and the new bone quality was better. The bone regeneration effect of odontogenic BCP was similar to the effect of deproteinized bovine bone mineral, but b- TCP did not maintain the height and volume of bone reconstruction. CONCLUSION: In conclusion, the combined application of DNCPs and odontogenic BCP is an effective strategy for tissue engineering osteogenesis in the maxillary sinus region. The biomimetic strategy could provide a new approach for patients requiring maxillary sinus lifting.

Multi-spectral Mueller Matrix Imaging for Wheat Stripe Rust

Yang Feng,Tianyu He,Wenyi Ren,Dan Wu,Rui Zhang,Yingge Xie 한국광학회 2024 Current Optics and Photonics Vol.8 No.2

Wheat stripe rust, caused by Puccinia striiformis, has reduced winter wheat yield globally for ages. In this work, multi-spectral Mueller matrix imaging with 37 measurements using the method of double rotatable quarter-wave plates was used to investigate wheat stripe rust. Individual Mueller matrix measurements were performed on incident monochromatic light with nine bands in the range of 430 to 690 nm. As a result, it was found that the infected area absorbed linearly polarized light and was sensitive to circularly polarized light in the spectral domain. Both linear depolarization and linear diattenuation images distinguished between wheat stripe rust and healthy tissue. The responsiveness of stripe rust to polarized light reveals the potential of using polarization imaging to detect plant diseases. This further suggests that the multi-spectral Mueller matrix imaging system provides us with an alternative approach to agricultural disease detection.

Histological Validation of Cardiovascular Magnetic Resonance T1 Mapping for Assessing the Evolution of Myocardial Injury in Myocardial Infarction: An Experimental Study

Zhang Lu,Yang Zhi-gang,Xu Huayan,Yang Meng-xi,Xu Rong,Chen Lin,Sun Ran,Miao Tianyu,Zhao Jichun,Zhou Xiaoyue,Fu Chuan,Guo Yingkun 대한영상의학회 2020 Korean Journal of Radiology Vol.21 No.12

Objective: To determine whether T1 mapping could monitor the dynamic changes of injury in myocardial infarction (MI) and be histologically validated. Materials and Methods: In 22 pigs, MI was induced by ligating the left anterior descending artery and they underwent serial cardiovascular magnetic resonance examinations with modified Look-Locker inversion T1 mapping and extracellular volume (ECV) computation in acute (within 24 hours, n = 22), subacute (7 days, n = 13), and chronic (3 months, n = 7) phases of MI. Masson’s trichrome staining was performed for histological ECV calculation. Myocardial native T1 and ECV were obtained by region of interest measurement in infarcted, peri-infarct, and remote myocardium. Results: Native T1 and ECV in peri-infarct myocardium differed from remote myocardium in acute (1181 ± 62 ms vs. 1113 ± 64 ms, p = 0.002; 24 ± 4% vs. 19 ± 4%, p = 0.031) and subacute phases (1264 ± 41 ms vs. 1171 ± 56 ms, p < 0.001; 27 ± 4% vs. 22 ± 2%, p = 0.009) but not in chronic phase (1157 ± 57 ms vs. 1120 ± 54 ms, p = 0.934; 23 ± 2% vs. 20 ± 1%, p = 0.109). From acute to chronic MI, infarcted native T1 peaked in subacute phase (1275 ± 63 ms vs. 1637 ± 123 ms vs. 1471 ± 98 ms, p < 0.001), while ECV progressively increased with time (35 ± 7% vs. 46 ± 6% vs. 52 ± 4%, p < 0.001). Native T1 correlated well with histological findings (R2 = 0.65 to 0.89, all p < 0.001) so did ECV (R2 = 0.73 to 0.94, all p < 0.001). Conclusion: T1 mapping allows the quantitative assessment of injury in MI and the noninvasive monitoring of tissue injury evolution, which correlates well with histological findings.

Ion-imprinted antifouling nanocomposite membrane for separation of lithium ion

Dongshu Sun,Tianyu Zhou,Yang Lu,Yongsheng Yan,Chunbo Liu,Guangbo Che 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.9

Membrane fouling is a primary challenge restricting the practical application of membrane separation technology. Inspired by the idea that improving hydrophilicity would lead to antifouling performance of membrane material. In this work, ion-imprinted antifouling nanocomposite membrane (LiI-NcMs) blended with GO and TiO2 nanomaterialwas fabricated for selective separation of lithium ion. As a result, markedly improved hydrophilicity was achievedon LiI-NcMs (55.1o for contact angle). LiI-NcMs also showed good selective adsorption capacity in the mixed solutionof Mg2+ and Li+. Moreover, LiI-NcMs exhibited superior stability; after 20 adsorption/desorption cycles a maximumadsorption capacity of 88.1% can be maintained. This work demonstrates a new and facile approach to prepare novelmembrane separation material for a particular substance for efficient selective separation in industrial applications.

Antidepressant-like effect of ginsenoside Rb1 on potentiating synaptic plasticity via the miR-134emediated BDNF signaling pathway in a mouse model of chronic stress-induced depression

Guoli Wang,Tianyue An,Cong Lei,Xiaofeng Zhu,Li Yang,Lianxue Zhang,Ronghua Zhang 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.3

Brain-derived neurotrophic factor (BDNF)etropomyosin-related kinase B (TrkB) plays acritical role in the pathogenesis of depression by modulating synaptic structural remodeling and functional transmission. Previously, we have demonstrated that the ginsenoside Rb1 (Rb1) presents a novelantidepressant-like effect via BDNFeTrkB signaling in the hippocampus of chronic unpredictable mildstress (CUMS)-exposed mice. However, the underlying mechanism through which Rb1 counteractsstress-induced aberrant hippocampal synaptic plasticity via BDNFeTrkB signaling remains elusive. Methods: We focused on hippocampal microRNAs (miRNAs) that could directly bind to BDNF and areregulated by Rb1 to explore the possible synaptic plasticity-dependent mechanism of Rb1, which affordsprotection against CUMS-induced depression-like effects. Results: Herein, we observed that brain-specific miRNA-134 (miR-134) could directly bind to BDNF 30UTRand was markedly downregulated by Rb1 in the hippocampus of CUMS-exposed mice. Furthermore, thehippocampusetargeted miR-134 overexpression substantially blocked the antidepressant-like effects ofRb1 during behavioral tests, attenuating the effects on neuronal nuclei-immunoreactive neurons, thedensity of dendritic spines, synaptic ultrastructure, long-term potentiation, and expression of synapseassociated proteins and BDNFeTrkB signaling proteins in the hippocampus of CUMS-exposed mice. Conclusion: These data provide strong evidence that Rb1 rescued CUMS-induced depression-like effectsby modulating hippocampal synaptic plasticity via the miR-134-mediated BDNF signaling pathway.

Simulated altitude exercise training damages small intestinal mucosa barrier in the rats

Li, Meng,Han, Tianyu,Zhang, Weijia,Li, Wei,Hu, Yang,Lee, Sang Ki Korean Society of Exercise Rehabilitation 2018 JER Vol.14 No.3

<P>This study investigated the effect of simulated altitude training on the changes of small intestinal mucosa barrier, bacterial overgrowth and inflammatory response in the small intestine of rat. Male 8-week-old Sprague-Dawley rats were randomly divided into four groups: normal oxygen sedentary group (n=30), normal oxygen exercise group (n=30), low oxygen sedentary group (n=30) and low oxygen exercise group (n=30). Exercise training was on a treadmill for 1 hr per day on days 3, 6, and 9 in the hypoxia condition. Hematological profiles, hematolxylin and eosin staining, fluorescence <I>in situ</I> hybridization, reverse transcription-polymerase chain reaction and Western blot were used to analyze the effect of simulated altitude training on the amount of bacteria, and expression of mRNA and protein. Simulated exercise training significantly increased red blood cells and hematocrit. The small intestinal mucosa barrier was significantly injured by the simulated altitude exercise training. Comparatively more bacterial growth was evident in the small intestine by the simulated altitude exercise training. mRNA levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) and protein expression of nuclear factor-kappa B (NF-κB) were significantly elevated by simulated altitude exercise training. These results suggest that the simulated altitude exercise training may impair the small intestinal mucosa barrier via elevation of bacterial growth and inflammatory cytokines (IL-6, TNF-α) and the up-regulation of NF-κB in the rats.</P>

Spatio-temporal Semantic Features for Human Action Recognition

( Jia Liu ),( Xiaonian Wang ),( Tianyu Li ),( Jie Yang ) 한국인터넷정보학회 2012 KSII Transactions on Internet and Information Syst Vol.6 No.10

Most approaches to human action recognition is limited due to the use of simple action datasets under controlled environments or focus on excessively localized features without sufficiently exploring the spatio-temporal information. This paper proposed a framework for recognizing realistic human actions. Specifically, a new action representation is proposed based on computing a rich set of descriptors from keypoint trajectories. To obtain efficient and compact representations for actions, we develop a feature fusion method to combine spatial-temporal local motion descriptors by the movement of the camera which is detected by the distribution of spatio-temporal interest points in the clips. A new topic model called Markov Semantic Model is proposed for semantic feature selection which relies on the different kinds of dependencies between words produced by “syntactic” and “semantic” constraints. The informative features are selected collaboratively based on the different types of dependencies between words produced by short range and long range constraints. Building on the nonlinear SVMs, we validate this proposed hierarchical framework on several realistic action datasets.

Essential oil of Citrus limon Osbeck: An insecticidal natural product against Cryptolestes pusillus SchÖnherr

Jie Yu,Shi Tianyu,Yang Dong,Zhang Zhongjie 한국응용곤충학회 2024 Journal of Asia-Pacific Entomology Vol.27 No.1

This study investigated the insecticidal activity and mechanism of action of essential oil from Citrus limon Osbeck (C. limon oil). The fumigation method was used to determine the insecticidal activity of different concentrations of C. limon oil against adult Cryptolestes pusillus (C. pusillus). In addition, the effect of C. limon oil on the organs of adult C. pusillus was studied. The results showed that when the concentration of C. limon oil reached 0.6 μL/cm 3 , following fumigation for 4, 8, 12, 16, 20, and 24 h, the corrected mortality rates of adult C. pusillus were 13 %, 30 %, 47 %, 95 %, 97 %, and 100 %, respectively. Furthermore, C. limon oil caused vacuolization in the digestive tract and adipose cells, thus, as a plant-based insecticide, it has strong research and application value.

Study on localization influences of frequency veering on vibration of mistuned bladed disk

Hongyuan Zhang,Huiqun Yuan,Wenjun Yang,Tianyu Zhao 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.11

Reduced-order models of the bladed disk of the aero-engine compressor were established by adopting Prestressed component mode synthesis (PCMS) method. The frequency veering characteristics of the tuned bladed disk were analyzed. From the aspect of the strain energy, the forced vibration response of the mistuned bladed disk was analyzed, along with calculation of the contribution degree factors, the localization factors and frequency veering distances, finding the influences of the frequency veering distance, the contribution degree factors and the localization. The results show that frequency veering has significant influence on vibration localization of the mistuned bladed disk; in the region of frequency veering, the degree of vibration localization of the mistuned bladed disk is relatively high; along with the changes of the frequency veering distance, the contribution degree of the strain energy of the blades of localization of the mistuned bladed disk shows the certainty of regularity.

High-Conductivity SiO2-Matrix B-Doped Si-NC Thin Films by Following Ion-Beam Treatment

Junjun Huang,Weiyan Wang,Jie Yang,Yongzhen Tan,Wei Chen,Tianyu Ge,Yajun Zhang,Min Gao,Zhenming Chen 대한금속·재료학회 2016 ELECTRONIC MATERIALS LETTERS Vol.12 No.6

In this work, further ion-beam was performed on SiO2-matrix B-doped Si-NC (SBC) thin films in order to enhance conductivity. The effect of ionbeamtype on the electrical properties of SBC thin films was investigatedsystematically. The results indicated that the conductivities of SBC thinfilms were significantly improved by both argon and hydrogen ion-beamtreatments, and the higher the hydrogen ion ratio, the higher theconductivity of SBC thin films. The conductivity of SBC thin films wasincreased from 1.82 × 10−6 S/cm to 3.2 × 10−3 S/cm with followinghydrogen-ion-beam treatment. The change in conductivity of SBC thinfilms was most possibly resultant from the ion-beam treatment facilitatingthe formation of higher superficial order and lower defects. An alternativemethod was proposed to prepare high-conductivity SBC thin films, whichmay be applied to other heterogeneous thin films.