http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Talmadge, James E. (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
-
재조합 인체 인터루킨 - II . 생물학적 및 면역요법적 활성도
최혜림,윤혜영,James E . Talmadge,함경수 ( Hye Lim Choi,Hye Young Yun,James E . Talmadge,Kyung Soo Hahm ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.2
Recombinant human interleukin-2 (rH IL-2, Ser^(125)-rH IL-2) purified from E. coli was characterized its biological and therapeutic activities for the establishment of preclinical screening system and therapeutic applications. In vitro augmentation of natural killer (NK) cell activity and mixed lymphocyte reaction (MLR) assay were shown to be comparable with other rH IL-2 from different manufacturers and laboratories. An antiserum against rH IL-2 was obtained from chick, and characterized the reacticvity with rH IL-2 reconstituted from lyophilized state in relation to the stability and conformational changes of the recombinant protein. In addition, long term storage of rH IL-2 in lyophilized or solution was not shown to incur remarkable loss of MLR activities, and any appearance of either degradation or aggregation products when analyzed by SDS-PAGE, indicating the stability and suitability for therapeutic applications of rH IL-2.
-
Recombinant Human Interleukin-2: II. Biological and Therapeutic Activities
최혜림,윤혜영,함경수,Choi, Hye-Lim,Yun, Hye-Young,Talmadge, James E.,Hahm, Kyung-Soo 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.2
Recombinant human interleukin-2 (rH IL-2, $Ser^{125}$-rH IL-2) purified from E. coli was characterized its biological and therapeutic activities for the establishment of preclinical screening system and therapeutic applications. In vitro augmentation of natural killer (NK) cell activity and mixed lymphocyte reaction (MLR) assay were shown to be comparable with other rH IL-2 from different manufacturers and laboratories. An antiserum against rH IL-2 was obtained from chick, and characterized the reacticvity with rH IL-2 reconstituted from lyophilized state in relation to the stability and conformational changes of the recombinant protein. In addition, long term storage of rH IL-2 in lyophilized or solution was not shown to incur remarkable loss of MLR activities, and any appearance of either degradation or aggregation products when analyzed by SDS-PAGE, indicating the stability and suitability for therapeutic applications of rH IL-2. 대장균으로 부터 분리정제된 재조합 인체 인터루킨-2 (KAIST rH IL-2, $Ser^{125}$-rH IL-2)를 전임상시험 및 암치료에 응용하기 위한 목적으로 생물학적 및 면역요법적 활성도를 측정하였다. In vitro에서 자연살해세포 활성의 증진능력 및 혼합 임파구반응 실험에서 활성을 나타냈으며 다른 회사의 재조합 인터루킨-2 제품들과 유사한 결과를 얻었다. 재조합 인체 인터루킨-2의 입체구조적 및 생물학적 활성도의 안정성을 조사하기 위하여 정제된 재조합 인체 인터루킨-2에 대한 항혈청을 닭에서 얻었으며 동결건조 저장하였던 재조합 인체 인터루킨-2와 이 항체와의 반응을 조사하였으며, 또한 6개월간 저장하였던 인터루킨-2의 안정성을 혼합 임파구반응 및 전기영동 방법으로 그 활성 및 단백질 성분을 조사한바 혼합임파구 반응에서 여전히 활성을 보여주였으며, 전기영동상 분해되었거나 응집된 산물이 생기지 않았음을 확인하였다. 이상의 결과는 KAIST 재조합 인체 인터루킨-2가 전임상시험 및 임상시험 목적에 사용하기에 적합함을 시사한다.
-
-
( Bo Hyung Kang ),( Joyce S Lee ),( Brett M Elicker ),( Thomas Urbania ),( Jay H Ryu ),( Talmadge E King ),( Harold R Collard ),( Dong Soon Kim ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-
Introduction: Definite usual interstitial pneumonia (UIP) pattern on high-resolution computed tomography (HRCT) is very specific, but not sensitive, for UIP pattern on surgical lung biopsy in patients suspected of having idiopathic pulmonary fibrosis (IPF). We investigated whether definite UIP pattern on HRCT was specific for UIP pattern on histopathology in patients with rheumatoid arthritis associated interstitial lung disease (RA-ILD). Methods: Two radiologists reviewed HRCT scans patients with biopsy proven RA-ILD. The radiologists categorized the HRCT scans as definite, possible or inconsistent with UIP pattern using the ATS/ERS/JRS/ALAT criteria. We also determined the sensitivity and specificity of definite UIP pattern on HRCT to UIP pattern onhistopathology. Results: There were 69 patients with RA-ILD included in this study and were collected from 3 tertiary care centers: University of California San Francisco, Mayo Clinic, and Asan Medical Center. The frequency of histopathologic UIP pattern in this cohort was 61%. Men were more likely than women to have a UIP pattern on histopathology (p 0.02). The agreement between the 2 radiologists for definite UIP pattern was 87% (kappa 0.67, p value <0.0001). The specificity of UIP pattern on HRCT for histopathologic UIP pattern was 96% (95% CI 81%-100%), with a positive predictive value of 95%. The sensitivity of UIP pattern on HRCT for histopathologic UIP pattern was 45% (95% CI 30%-61%), with a negative predictive value of 53%. Conclusion: Definite UIP pattern on HRCT in patients with RA-ILD is highly specific, but not sensitive, to UIP pattern on histopathology. This is consistent with the literature in IPF.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
