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Chan Soo Moon,Yun Jung Choi,Eun Young Kim,In Sun Lee,Sae Byol Kim,Sung Mo Jung,Se Kyu Kim,Joon Chang,Ji Ye Jung 대한결핵 및 호흡기학회 2013 Tuberculosis and Respiratory Diseases Vol.74 No.3
Splenosis is defined as an autotransplantation of the splenic tissue after splenic rupture or splenectomy, and occurs most frequently in the peritoneal cavity. Splenosis is usually asymptomatic and is found incidentally. We report a case of combined intrathoracic and intraperitoneal splenosis in a 54-year-old male who worked as a miner for 10 years in his twenties, and was a current smoker. He was referred to our hospital for further evaluation of an incidental left diaphragmatic mass. Positron emission tomography-computed tomography and bronchoscopy were performed to evaluate the possibility of malignancy. There was no evidence of malignancy, but the spleen was not visualized. Reviewing his medical history revealed previous splenectomy, following a dynamite explosion injury. Therefore, splenosis was suspected and technetium-99m-labeled heat-damaged red blood cell scan confirmed the diagnosis. Radionuclide imaging is a useful diagnostic tool for splenosis, which could avoid unnecessary invasive procedures.
Chan-Jung Lee,Hye-Su Han,Chang-Sung Jhune,Jong-Chun Cheong,Jin-A Oh,Won-Sik Kong 한국버섯학회 2011 한국버섯학회지 Vol.9 No.4
Cobweb disease symptoms were observed in a mushroom farm in Buye, Korea during a disease survey in 2008-2011. Five isolates of Cladobotryum sp. were obtained from the infected caps and stipes. These isolates of Cladobotryum sp. were identified as C. mycophilum based on their morphological, cultural characteristics and analysis of the ITS sequences. Early symptoms were noticed as round, fleshy, yellowish brown lesions on mushroom caps. Late symptoms progressed when the parasitic fungus formed white cobweb circular colonies on dead or damaged pinheads, spread on the surface of the casing, and covered entirely fruiting bodies. Optimal temperature and pH for mycelial growth on MEA is 23℃ and 6.0. Microscopically the spores of the fungus are large and most 2~3 celled produced on vertically branched conidiophores. Mushroom caps turned dark brown and shrunk due to soft rot. Testing of sensitivity to selected fungicides showed that isolate was highly resistance to Mancozeb and Thiophanate-methyl, moderately sensitivity to Iprodione, and highly sensitivity to Benomyl, Prochloraz-Mn and Carbendazim.
Rapid, sensitive and specific detection of Brucella canis by real-time PCR assay using HybProbe
( Sung Ll Kang ),( Moon Her ),( Ji Yeon Kim ),( Kichan Lee ),( Jong Wan Kim ),( Hyang Keun Lee ),( So Ra Sung ),( Hyorim Cho ),( Suk Chan Jung ) 한국예방수의학회(구 한국수의공중보건학회) 2013 예방수의학회지 Vol.37 No.1
We designed and evaluated a specific HybProbe set for identification of Brucella canis, which is based on a specific single nucleotide polymorphism (SNP) of the omp25 gene. Real-time PCR assay using this probe specifically identified B. canis reference and field strains, however, the other Brucella species and general bacteria strains were not amplified. This real-time PCR assay can re-confirm specific amplification in melting curve analysis. The average melting temperature values for generation of B. canis strains wan 67℃m, due to a single base-pair mismatch at the binding site of the probe. The detection limit appeared to be 30 fg of DNA and 100CFU by serial dilution of whole blood inoculated with B. canis. In addition, use of the buffy coat resulted in approximately 50 times higher sensitivity than whole blood directly. This technique is widely applicable for use in diagnostic laboratories and is readily discriminative with regard to small amounts of biomass.
Sung, Ha-Cheol,Kim, Su-Kyung,Cheong, Seok-Wan,Park, Shi-Ryong,Roh, Dong-Chan,Baek, Kyung-Whan,Lee, Jung-Hyun,Park, Dae-Sik The Ecological Society of Korea 2006 Journal of Ecology and Environment Vol.29 No.4
We investigated the distribution of three anuran species, Three-striped pond frogs (Rana nigromaculata), Bullfrogs (Rana catesbeiana), and Narrow-mouthed toads (Kaloula borealis), in an administrative district, Haenam Gun, Junnam Province, Korea using volunteer call surveys. Twenty-eight volunteer call surveyors were assigned to each $2{\times}2km^2$ survey plot. Call surveys on whether the species are present or not were conducted for 5 minutes between 30 minutes after sunset and the midnight on rice fields and ponds from 10 April to 28 August in 2005. Depending on species, call surveys were carried out at seven to 28 plots with average 8.4 to 10.7 visits per the plot. We calculated the detection probabilities and occupancy rates of the three species using four models with three covariates: temperature, humidity, and the amount of water at the habitat. The model average detection probabilities of three anuran species of R. nigromaculata, R. catesbeiana, and K. borealis were 0.53, 0.74, and 0.41 respectively, and the site occupancy rates of them were 0.93, 0.94, and 0.86 respectively. Our results indicate that R. nigromaculata, R. catesbeiana, and K. borealis are common in Haenam Gun.
( Chan Ran You ),( Myeong Jun Song ),( Chan Kwon Jung ),( Sung Woo Hong ),( Won Hee Hur ),( Sang Wook Choi ),( Si Hyun Bae ),( Jong Young Choi ),( Seung Kew Yoon ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1
Background: Ribosomal proteins (RP) involve in the regulation of apoptosis and carcinogenesis. In recent studies of RP in hepatocellular carcinoma (HCC), the overexpression of ribosomal protein L12, L27, L30 and L36 was found. We aimed to investigate the expression of ribosomal protein S23 (RPS23) and its effect on prognosis in postoperative HCC patients. Methods: Liver tissues were obtained 63 patients who had undergone curative resection for HCC. We enrolled the patients with early tumor stage (≤ UICC stage III) and good liver function (≤ Child Pugh score 6). The expressions of RPS23 in tumor tissue and non-tumor tissue were examined by immunohistochemical staining. Analyses for survival and recurrence were done between tumor RPS23 (t-RPS23) expression group and non-expression group. Results: RPS23 was expressed in 34 patients (54.0%) on tumor tissue and 8 patients (13.1%) in Non-tumor tissues (P=0.016). The patients were grouped according to the expression of RPS26 in tumor tissue. Basal characteristics were not different between t-RPS23 positive and negative group. The three years and five years survival rate was higher in t-RPS23 positive group (90.2% vs. 65.8% of 3 year survival rate, P=0.007, 86.1% vs. 55.7% of 5 year survival rate, P=0.010). On multivariate analysis, t-RPS23 expression (HR 10.95, P=0.004) and tumor number (HR 9.71, P=0.001) were independent prognostic factors for 3 year and 5 year survival. Tumor recurrence during one year after resection was relatively low in t-RPS23 positive group, but not statistically significant (17.8% vs. 36.0%, P=0.084). However, on multivariate analysis, t-RPS23 expression (HR 4.18, P=0.021) and serosa invasion (HR 4.15, P=0.019) were independent factors for 1 year tumor recurrence. Conclusions: The RPS23 expression of HCC patients was higher in tumor tissue than in non- tumor tissues. The RPS23 expression in tumor tissue was good prognostic marker for resected HCC. RPS23 may be associated with good oncogene to have paradoxical function in HCC.
Determination of Physicochemical Properties and Pharmacokinetic Profiles of Soybean Extracts
Jung, Hyun-Chan,You, Sung-Kyun,Kwon, Sun-Kyu,Hwang, Ji-Sook,Cho, Cheong-Weon The Korean Society of Pharmaceutical Sciences and 2010 Journal of Pharmaceutical Investigation Vol.40 No.6
Isoflavones have received much attention because of their health-related and clinical benefits such as estrogenic and anti-oxidative activities as well as triggering of natural killer cell activity. However, there are few publications reporting the pharmacokinetic profiles together with physicochemical properties of main isoflavones. Therefore, the pharmacokinetic parameters of main aglycones, daidzein, glycitein and genistein after oral administration of soybean extracts were investigated and the physicochemical properties of soybean extracts were characterized. It was observed that angle of repose was $46^{\circ}$ and tap density, bulk density and porosity were 10.12, 4.3 and $0.86\;g/cm^3$ and the mean $AUC_{last}$ of daidzein, glycitein and genistein was $11.376\;{\mu}g{\cdot}h/mL$, $3.045\;{\mu}g{\cdot}h/mL$ and $0.825\;{\mu}g{\cdot}h/mL$, respectively. Cell viability was 60% at a concentration of 10 mg/mL. Taken together, it was suggested that isoflavones were contained in the soybean products and had an antioxidant activity and this study would be the basis to control the quality of soybean products and study of the bioequivalence between soybean products in future.