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Culture supernatant proteins of Brucella canis represented potential antigenicity
Jin Ju Lee(Jin Ju Lee),So-Ra Sung(So-Ra Sung),Eun Ji Yum(Eun Ji Yum),Sung Dae Yang(Sung Dae Yang),Min Hoe Lee(Min Hoe Lee),Bang-Hun Hyen(Bang-Hun Hyen),Moon Her(Moon Her),Hee Soo Lee(Hee Soo Lee) 한국예방수의학회 2018 한국예방수의학회 학술대회자료집 Vol.2018 No.-
Lee, Sung Hyen,Lillehoj, Hyun S.,Jang, Seung I.,Lillehoj, Erik P.,Min, Wongi,Bravo, David M. Cambridge University Press 2013 The British journal of nutrition Vol.110 No.5
<P>The <I>Clostridium</I>-related poultry disease, necrotic enteritis (NE), causes substantial economic losses on a global scale. In the present study, a mixture of two plant-derived phytonutrients, <I>Capsicum</I> oleoresin and turmeric oleoresin (XT), was evaluated for its effects on local and systemic immune responses using a co-infection model of experimental NE in commercial broilers. Chickens were fed from hatch with a diet supplemented with XT, or with a non-supplemented control diet, and either uninfected or orally challenged with virulent <I>Eimeria maxima</I> oocysts at 14 d and <I>Clostridium perfringens</I> at 18 d of age. Parameters of protective immunity were as follows: (1) body weight; (2) gut lesions; (3) serum levels of <I>C. perfringens</I> α-toxin and NE B-like (NetB) toxin; (4) serum levels of antibodies to α-toxin and NetB toxin; (5) levels of gene transcripts encoding pro-inflammatory cytokines and chemokines in the intestine and spleen. Infected chickens fed the XT-supplemented diet had increased body weight and reduced gut lesion scores compared with infected birds given the non-supplemented diet. The XT-fed group also displayed decreased serum α-toxin levels and reduced intestinal <I>IL-8</I>, lipopolysaccharide-induced TNF-α factor (<I>LITAF</I>), <I>IL-17A</I> and <I>IL-17F</I> mRNA levels, while cytokine/chemokine levels in splenocytes increased in the XT-fed group, compared with the animals fed the control diet. In conclusion, the present study documents the molecular and cellular immune changes following dietary supplementation with extracts of <I>Capsicum</I> and turmeric that may be relevant to protective immunity against avian NE.</P>
( Sang-yeop Lee ),( Won-seok Yang ),( Se-won Park ),( Gun-ho Han ),( Yong-chil Seo ),( Woo-hyen Kim ),( Young-sik Yoon ),( Won-sik Lee ) 한국폐기물자원순환학회(구 한국폐기물학회) 2019 한국폐기물자원순환학회 심포지움 Vol.2019 No.1
Solid refuse fuel (SRF) manufacturing facilities, residues are buried at a rate of 30~45% of incoming amount or some are disposed of by incineration facilities. It is known that landfill can increase the pollution load and it takes about 20% of total facility operation cost due to landfill disposal cost. In the study residue waste from SRF manufacturing plant was attempted to combust, the bottom ash generated from incineration tests was utilized aggregates to fabricate bricks to assess the applicability. The variation in bottom ash properties influences the characteristics of bricks and also it could be hazardous for environment. So, before making bricks from generated bottom ash, basic characteristic of bottom ash such as ignition loss and heavy metal leaching test must be analyzed. These bricks were manufactured by substitution of stone powder and sand. These can be used as an interlocking block for the sidewalk and open space. Finally we analyzed the basic characteristics of the bottom ash and analyzed the water absorption, bending strength, compressive strength, and dimensions of the mixed bricks. Following these results, as comparing with standard interlocking block, recycling brick satisfied with the less than 10% abosrption rate and more than 5Mpa flexural strenghth. we could assess the applicability of these bricks manufactured.
Lee, Min Ai,Park, Hyen Joo,Chung, Hwa-Jin,Kim, Won Kyung,Lee, Sang Kook American Chemical Society and American Society of 2013 Journal of natural products Vol.76 No.7
<P>The antiproliferative and antitumor activities of 2-hydroxycinnamaldehyde (<B>1</B>), a phenylpropanoid isolated from the bark of <I>Cinnamomum cassia</I>, were investigated using human colorectal cancer cells. Compound <B>1</B> exhibited antiproliferative effects in HCT116 colon cancer cells, accompanied by modulation of the Wnt/β-catenin cell signaling pathway. This substance was found also to inhibit β-catenin/T-cell factor (TCF) transcriptional activity in HEK293 cells and HCT116 colon cancer cells. Further mechanistic investigations in human colon cancer cells with aberrantly activated Wnt/β-catenin signaling showed that <B>1</B> significantly suppressed the binding of β-catenin/TCF complexes to their specific genomic targets in the nucleus and led to the down-regulation of Wnt target genes such as c-myc and cyclin D1. In an in vivo xenograft model, the intraperitoneal administration of <B>1</B> (10 or 20 mg/kg body weight, three times/week) for four weeks suppressed tumor growth in athymic nude mice implanted with HCT116 colon cancer cells significantly, without any apparent toxicity. In an ex vivo biochemical analysis of the tumors, compound <B>1</B> was also found to suppress Wnt target genes associated with tumor growth including β-catenin, c-myc, cyclin D1, and survivin. The suppression of the Wnt/β-catenin signaling pathway is a plausible mechanism of action underlying the antiproliferative and antitumor activity of <B>1</B> in human colorectal cancer cells.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2013/jnprdf.2013.76.issue-7/np400216m/production/images/medium/np-2013-00216m_0007.gif'></P>
Sang Hun Lee(이상훈),Ki Yuol Jung(정기열),Hyen Chung Chun(전현정),Dong Hyok Gong(공동혁) 한국토양비료학회 2021 한국토양비료학회 학술발표회 초록집 Vol.2021 No.11
Subsurface drip irrigation is highly efficient irrigation system to save water compared to surface irrigation methods. Although the use of soil sensors helps farmers with irrigation scheduling by providing information about when and how much to irrigate, little information is available on soil moisture pattern by subsurface drip irrigation using different soil sensors. Therefore, irrigation scheduling using different soil sensors was evaluated to compare soil moisture wetting pattern at coarse textured soil. Subsurface drip irrigation system was installed at 40cm depth from soil surface with the dripline of 1.6L/hr effluent rate. Study soil has 6% clay content and classified with sandy loam, which was evaluated plant available water content of 10.8%. Subsurface drip irrigation were automatically initiated based on soil moisture sensor and tensiometer. In this study soil water content was fluctuated with irrigation and precipitation event. In the treatment of irrigation by soil moisture sensor, soil water content was well maintained at desired soil water content. However, the variability of soil water content by tensiometer was relatively greater compared to soil water sensor. The results of this study indicate that the irrigation rate and coverage of the irrigation system should be determined on irrigated soil and the amount of water to be applied or target point must be determined based on the irrigation system and the soil water characteristics.