Inhibitory Effects of (-)Epigallocatechin Gallate and Quercetin on Phorbol 12-Myristate 13-Acetate-Induced Secretion of Metalloproteinase-2 and Metalloproteinase-9

Kang Sang-Wook,Choi Yean-Jung,Choi Jung-Suk,Kwon Hyang-Mi,Bae Ji-Young,Park Eun-Hee,Ji Geun-Eog,Kang Il-Jun,Kang Young-Hee The Korean Nutrition Society 2006 Nutritional Sciences Vol.9 No.3

Matrix metalloproteinases (MMP) play an important role in the extracellular matrix (ECM) degradation undetphysiological and pathological conditions. The present study examined the influence of (-)epigallocatechin gallate and quercetin on phorbol-12-myristate 13-acetate (PMA)-induced secretion of MMP-2 and MMP-9, when human umbilical vein endothelial cells (HUVEC) were treated with (-)epigallocatechin gallate and quercetin at supraphysiological concentrations of $25{\mu}mol/L$. No cytotoxicity was observed by MIT assay in response to a treatment with PMA in the presence of (-)epigallocatechin gallate and quercetin. Western blot analysis and gelatin zymography revealed that exposure of HUVEC to PMA enhanced the levels and gelatinolytic activities of pro and active forms of MMP-2 and active form of MMP-9. (-)Epigallocatechin gallate attenuated PMA-stimulated secretion of active forms of MMP-2 and MMP-9 concomitantly with a loss of activities of these enzymes, which was related to the decreased mRNA levels of MMP. Quercetin was more potent than (-)epigallocatechin gallate in alleviating MMP-9 protein secretion and activity with a decrease in MMP-9 mRNA accumulation. Taken together, the results indicated that (-)epigallocatechin gallte and quercetin exhibited inhibitory effects on MMP activity and may qualify as chemopreventive and cardiovascular protective agents.

The effect of extract from leaves and stalks of Angelica gigas on the innate immunity

Kang, Shin-Seok,Byeon, Hyeon-Seop,Kang, Shin-Kwon,Ko, Duk-Hwan,Lim, Dae-Jun,Lee, Jung-Hwa,Kang, Sung-Ho The Korean Society of Veterinary Service 2013 韓國家畜衛生學會誌 Vol.36 No.4

The dried root of Angelica gigas (A. gigas) has been traditionally used as an oriental medicine, which is known to improve blood circulation and blood stasis. In the present study, leaves and stalks of A. gigas were used to investigate their effects on the innate immunity. The extracts were prepared from leaves and stalks of A. gigas and were fed to mice. The numbers of blood cells, total WBCs, neutrophils, lymphocytes, eosinophils and basophils were increased by 50% in mice fed with leaves extract of A. gigas compared to control mice. However, the numbers of blood cells were decreased when treated with stalks extract of A. gigas. The level of cholesterol and triglyceride in serum was markedly reduced in both mice group fed with leaves extract and stalks extract of A. gigas compared to control group (P<0.01). There was no significant change in the level of albumin, total protein, phosphate and calcium in serum. Activity of cationic peptide was found to be diffused in the testicles of mice fed with leaves extract of A. gigas compared to control group, which might be due to increased lysozyme in testicle. The lysoplate assay and immunohistochemistry assay suggest that the extract of leaves and stalks of A. gigas are immunogenic, but the effects might be related with acquired immune response rather than innate immunity.

X-ray Crystal Structure and Luminescence Properties of Pd(II) and Pt(II) Complexes with Dithiopyrrole

Kang, Jun-Gill,Cho, Dong-Hee,Park, Changmoon,Kang, Sung Kwon,Kim, In Tae,Lee, Sang-Woo,Lee, Ha-Hyeong,Lee, Young-Nam,Lim, Dae-Won,Lee, Sung-Jae,Kim, Sung-Ho,Bae, Young-Ju Korean Chemical Society 2008 Bulletin of the Korean Chemical Society Vol.29 No.3

The complexes Pd(nbmtp)Cl2 and Pt(nbmtp)Cl2 (nbmptp = 1-nonyl-3,4-bis(methylthio)pyrrole) were prepared and their x-ray structures were determined at room temperature. The four-coordinated metal unit and the pyrrole ring formed a nearly planar geometry. The free ligand dissolved in CH2Cl2 produced two luminescence bands associated with the lone-pair electron of S (l max = 525 nm) and the pyrrole p electron (l max = 388 nm). When the two complexes were dissolved in CH2Cl2, these two luminescence bands were also observed, although the low-energy band was blueshifted. For the crystalline Pt(II) complex, only the strong charge transfer band (l max = 618 nm) from the d* orbital of Pt resulted from excitation of the lone-pair electron of S.

NMR-Based Metabolomics Approach for the Differentiation of Ginseng (Panax ginseng) Roots from Different Origins

Kang, Jin-Ho,Lee, Seo-Young,Kang, Sun-Mi,Kwon, Hyuk-Nam,Park, Jeong-Hill,Kwon, Sung-Won,Park, Sung-Hyouk 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.3

Agro-herbal materials vary in prices and qualities depending on the origin and age and the differentiation is both scientific and public health issue. Here, we describe a metabolomics approach used to discriminate ginseng roots from different sources. Six different types of ginseng roots from China and Korea were analyzed by NMR-based metabolomics. Chinese Dangsam showed prominent differences and was easily differentiated. The difference was mainly due to the large signals in the sugar region. We further analyzed the metabolomics results in subgroups. Jeonra (Korean), Choongcheong (Korean), and Chinese ginseng in subgroup 1 could be easily differentiated by the first two principal components. The loading plot for PC1 showed that the Jeonra and Chinese ginseng roots were mainly separated by sugar signals and methyl signals but that they were reverse-correlated. A diffusion-ordered spectroscopy (DOSY) analysis showed that the methyl signals are from high molecular weight compounds and that the sugar signals are either from oligosaccharides or ginsenosides. In subgroup 2, composed of Korean Choongcheong ginseng at different ages, we were able to see age-dependent transitions in the score plot. We believe our approach can be applied to detecting the adulteration of ginseng root powders and other herbal products from different origins.

Bioequivalence of Cholicerin Soft Capsule to Gliatilin Soft Capsule (Choline Alphoscerate 400 mg)

Kang, Hyun-Ah,Kim, Se-Mi,Kang, Seung-Rae,Kang, Min-Sun,Lee, Sang-No,Kwon, In-Ho,Yoo, Hee-Doo,Kim, Yoon-Gyoon,Lee, Yong-Bok The Korean Society of Pharmaceutical Sciences and 2010 Journal of Pharmaceutical Investigation Vol.40 No.2

The purpose of the present study was to evaluate the bioequivalence of two choline alphoscerate soft capsules, Gliatilin soft capsule (Daewoong Pharmaceuticals Co., Ltd.) and Cholicerin soft capsule (Sam Chun Dang Pharm. Co., Ltd.), according to the guidelines of Korea Food and Drug Administration (KFDA). Serum concentrations of choline after oral administration of choline alphoscerate were determined using a validated LC/MS/MS method. This method showed linear response over the concentration range of 0.5-20 ${\mu}g$/mL with correlation coefficient of 0.9999. The lower limit of quantitation using 100 ${\mu}L$ of serum was 0.5 ${\mu}g$/mL which was sensitive enough for pharmacokinetic studies. Thirty six healthy male Korean volunteers received each medicine at the choline alphoscerate dose of 1200 mg in a $2{\times}2$ crossover study. There was a one-week washout period between the doses. Blood samples were taken at predetermined time intervals up to 8 hr. $AUC_t$ (the area under the serum concentration-time curve from time 0 to 8 hr) was calculated by the linear trapezoidal rule method. $C_{max}$ (the maximum serum drug concentration) and $T_{max}$ (the time to reach $C_{max}$) were compiled from the serum concentration-time data. Analysis of variance was carried out using logarithmically transformed $AUC_t$ and $C_{max}$. No significant sequence effect was found for all of the bioavailability parameters, indicating that the crossover design was properly performed. The 90% confidence intervals of the $AUC_t$ ratio and the $C_{max}$ ratio for Cholicerin/Gliatilin were log0.9998-log1.1172 and log0.9938-1.0944, respectively. These values were within the acceptable bioequivalence intervals of log0.80-log1.25. Thus, the criteria of the KFDA guidelines for the bioequivalence was satisfied, indicating Cholicerin soft capsule and Gliatilin soft capsule are bioequivalent.

Oncologic outcomes of preoperative stent insertion first versus immediate surgery for obstructing left-sided colorectal cancer

Kang, Sung Il,Oh, Heung-Kwon,Yoo, Jae Suk,Ahn, Soyeon,Kim, Min Hyun,Kim, Myung Jo,Son, Il Tae,Kim, Duck-Woo,Kang, Sung-Bum,Park, Young Soo,Yoon, Chang Jin,Shin, Rumi,Heo, Seung Chul,Lee, In Taek,Youk, Elsevier 2018 Surgical oncology Vol.27 No.2

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Colonic self-expanding metallic stenting (SEMS) is widely used for the treatment of malignant colonic obstruction as a bridge to elective surgery. However, the effects of colonic stenting on long-term oncologic outcomes are debatable. This study aimed to compare the long-term oncologic outcomes of preoperative SEMS insertion with those of immediate surgery in patients with obstructing left-sided colorectal cancer.</P> <P><B>Methods</B></P> <P>A cohort of consecutive patients who underwent radical surgery for obstructing left-sided colorectal cancer between 2004 and 2011 in five tertiary referral hospitals were analyzed. Long-term survivals were analyzed and adjusted using the inverse probability of treatment weighting method, based on propensity scores, to reduce selection bias.</P> <P><B>Results</B></P> <P>One hundred and nine patients underwent immediate surgery, and 226 underwent stent insertion before surgery. Disease-free survival did not differ significantly in both the unadjusted population (hazard ratio [HR] 1.063, 95% confidence interval [CI] 0.730–1.548; Log-rank, p = 0.746) and the adjusted population (HR 0.122, 95% CI 0.920–1.987; Log-rank, p = 0.122). Overall survival also did not differ significantly in both the unadjusted population (HR 0.871, 95% CI 0.568–1.334; Log-rank, p = 0.526) and the adjusted population (HR 1.023, 95% CI 0.665–1.572; Log-rank, p = 0.916). Defunctioning stoma formation was less in the SEMS insertion group than immediate surgery group (adjusted, 14.6% vs. 41.3%, p < 0.001).</P> <P><B>Conclusion</B></P> <P>The ‘bridge to surgery’ strategy using metallic stents was oncologically comparable to immediate surgery in patients with malignant left-sided colorectal obstruction.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Disease-free survival rate was comparable, SEMS insertion vs. immediate surgery. </LI> <LI> Overall survival rate was comparable between the two groups. </LI> <LI> Defunctioning stoma formation was less in the SEMS insertion group. </LI> <LI> The ‘bridge to surgery’ strategy using stent insertion was oncologically acceptable. </LI> </UL> </P>

Performance of a Novel CT-Derived Fractional Flow Reserve Measurement to Detect Hemodynamically Significant Coronary Stenosis

Kang Si-Hyuck,Kim Soo-Hyun,Kim Sun-Hwa,Chun Eun Ju,Chung Woo-Young,Yoon Chang-Hwan,Park Sang-Don,Nam Chang-Wook,Kwon Ki-Hwan,Doh Joon-Hyung,Byun Young-Sup,Bae Jang-Whan,Youn Tae-Jin,Chae In-Ho 대한의학회 2023 Journal of Korean medical science Vol.38 No.32

Background: Fractional flow reserve (FFR) based on computed tomography (CT) has been shown to better identify ischemia-causing coronary stenosis. However, this current technology requires high computational power, which inhibits its widespread implementation in clinical practice. This prospective, multicenter study aimed at validating the diagnostic performance of a novel simple CT based fractional flow reserve (CT-FFR) calculation method in patients with coronary artery disease. Methods: Patients who underwent coronary CT angiography (CCTA) within 90 days and invasive coronary angiography (ICA) were prospectively enrolled. A hemodynamically significant lesion was defined as an FFR ≤ 0.80, and the area under the receiver operating characteristic curve (AUC) was the primary measure. After the planned analysis for the initial algorithm A, we performed another set of exploratory analyses for an improved algorithm B. Results: Of 184 patients who agreed to participate in the study, 151 were finally analyzed. Hemodynamically significant lesions were observed in 79 patients (52.3%). The AUC was 0.71 (95% confidence interval [CI], 0.63–0.80) for CCTA, 0.65 (95% CI, 0.56–0.74) for CT-FFR algorithm A (P = 0.866), and 0.78 (95% CI, 0.70–0.86) for algorithm B (P = 0.112). Diagnostic accuracy was 0.63 (0.55–0.71) for CCTA alone, 0.66 (0.58–0.74) for algorithm A, and 0.76 (0.68–0.82) for algorithm B. Conclusion: This study suggests the feasibility of automated CT-FFR, which can be performed on-site within several hours. However, the diagnostic performance of the current algorithm does not meet the a priori criteria for superiority. Future research is required to improve the accuracy.

HBV : PE-030 ; Epidemiologic review of patients with chronic hepatitis B in the era of antiviral therapy: A single center experience at 2010

( Kang Pyo Kim ),( Nam Woo Kwon ),( Beom Kyung Kim ),( Seung Up Kim ),( Do Young Kim ),( Sang Hoon Ahn ),( Kwang Hyub Han ),( Chae Yoon Chon ),( Jun Yong Park ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background: With wide use of vaccination and antiviral agents against hepatitis B virus (HBV) infection, epidemiologic characteristics of HBV-infected patients have changed substantially for several decades in South Korea, however, recent data are lacking. Hence, we reviewed the recent clinical characteristics of patients with chronic hepatitis B (CHB). Methods: From January 2010 to December 2010, we studied 5,262 consecutive HBV-infected patients who visited in Severance Hospital. Data concerning demographic profiles, laboratory findings about viral activity, and antiviral therapy were gathered. Results: Among the whole population, the median age was 51.0 and 66.0% were male. 1,788 (34.0%) had positive HBeAg and the median ALT level was 31.0 (2, 4700) IU/ml and 1,560 (29.6%) patients had family history of HBV-related liver disease. Regarding viral replication status, 297 (5%) patients were in immune tolerance phase, 2,195 (42%) in inactive phase, and the remaining 2,770 (53%) patients were in active CHB (either immune clearance or reactivation phase). Liver cirrhosis was diagnosed in 2,065 patients, among them, 611 (29.6%) patients had positive HBeAg, and 576 patients had experienced decompensation events including ascites, variceal bleeding, and hepatic encephalopathy. Hepatocellular carcinoma was diagnosed in 1,268 patients. Patients with HCC increased as they get older, especially after age of forty for male and after fifty for female. The male patients had a significantly higher ratio of the HCC than female patients did (78.7% vs. 21.3%, respectively, p<0.001). Conclusions: The study result shows that epidemiologic characteristics of CHB are male, elder, HBeAg negative dominant. The further studies are required to investigate the overall epidemiology of HBV infection in Korea.

Dengue Fever Mimicking Acute Appendicitis : A Case Report

Kang, Yoon Jung,Choi, Sung Youn,Kang, Im Ju,Lee, Jae Eun,Seo, Mi Hye,Lee, Tae Houn,Ghim, Byoung Kwon 대한감염학회 2009 감염과 화학요법 Vol.41 No.4

We experienced a case of enteritis mimicking acute appendicitis in Dengue fever. This is the first case report of such complication in Korea. A 36-year-old man presented with fever and abdominal pain after a trip to the Philippines. He complained of severe pain on the right iliac fossa region. Complete blood cell count showed thrombocytopenia with leucopenia. Computed tomogram (CT) of abdomen and pelvis revealed a normal appendix. Dengue fever was confirmed by IgM capture enzyme-linked immunosorbent assay against dengue virus. During the follow-up period of 4 weeks, he was recovered and platelet count gradually. We can see from this case that dengue fever may present with abdominal pain, which mimics acute appendicitis. Early recognition of dengue fever mimicking appendicitis is important to prevent unnecessary surgical intervention.

Effects of Gait Training Using Functional Electrical Stimulation on Stroke Patients' Balance and Gait Velocity

Kang, Kwon-Young,Choi, Wan-Suk,An, Ho-Jung,Koo, Ja-Pung,Lee, Joon-Hee,Yun, Young-Dae,Lee, Jung-Sook,Jung, Joung-Youl,Lee, Sang-Bin International Academy of Physical Therapy Research 2011 Journal of International Academy of Physical Ther Vol.2 No.2

The purpose of this study is to examine the effects of gait training using functional electrical stimulation on the improvement of hemiplegic patients' functions for balance and gait velocity. The subjects of the experiment were determined to be 10 each hemiplegic patients who had been diagnosed with stroke or brain damage six months or longer earlier assigned to an experimental group and a control group respectively. The subjects were evaluated before the experiment using Tetrax and 10M gait tests, received gait training five times a week for four weeks using functional electrical stimulation and were evaluated after the experiment in the same method as used in the evaluation before the experiment. In order to examine differences between the experimental group that received gait training using functional electrical stimulation and the control group that was treated by functional electrical stimulation and received gait training thereafter, differences between before and after the experiment were analyzed using paired sample t-tests and differences in changes after the experiment between the experimental group and the control group were analyzed using independent sample t-tests in order to compare the two groups with each other. Experimental results showed significant differences in weight bearing, balance and gait velocity between before and after the experiment in the experimental group(p<.05). In the control group, whereas weight bearing and gait velocity did not show any significant difference between before and after the experiment(p>.05), balance showed significant differences(p<.05). Weight bearing, balance and gait velocity change rates showed significant differences between the experimental group and the control group(p<.05). In conclusion, it was indicated that gait training using functional electrical stimulation is effective for enhancing stroke patients' weight bearing rates, balance abilities and gait velocity.