Dynamic analysis of Ca^(2+) level during bovine oocytes maturation and early embryonic development

Su Li Liang,Qian Jun Zhao,Xiang Chen Li,Ya Ping Jin,Yi Peng Wang,Xiao Hua Su,Wei Jun Guan,Yue Hui Ma 대한수의학회 2011 Journal of Veterinary Science Vol.12 No.2

Mammalian oocyte maturation and early embryo development processes are Ca^(2+)-dependent. In this study, we used confocal microscopy to investigate the distribution pattern of Ca^(2+) and its dynamic changes in the processes of bovine oocytes maturation, in vitro fertilization (IVF), parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) embryo development. During the germinal vesicle (GV) and GV breakdown stage, Ca^(2+) was distributed in the cortical ooplasm and throughout the oocytes from the MI to MII stage. In IVF embryos, Ca^(2+)was distributed in the cortical ooplasm before the formation of the pronucleus. In 4-8 cell embryos and morulas, Ca^(2+) was present throughout the blastomere. In PA embryos, Ca^(2+) was distributed throughout the blastomere at 48 h, similar to in the 4-cell and 8-cell phase and the morula. At 6 h after activation, there was almost no distribution of Ca^(2+) in the SCNT embryos. However, Ca^(2+) was distributed in the donor nucleus at 10 h and it was distributed throughout the blastomere in the 2-8 cell embryos. In this study, Ca^(2+) showed significant fluctuations with regularity of IVF and SCNT groups, but PA did not. Systematic investigation of the Ca^(2+) location and distribution changes during oocyte maturation and early embryo development processes should facilitate a better understanding of the mechanisms involved in oocyte maturation, reconstructed embryo activation and development, ultimately improving the reconstructed embryo development rate.

Prognostic Value of PLCE1 Expression in Upper Gastrointestinal Cancer: a Systematic Review and Meta-analysis

Cui, Xiao-Bin,Peng, Hao,Li, Su,Li, Ting-Ting,Liu, Chun-Xia,Zhang, Shu-Mao,Jin, Ting-Ting,Hu, Jian-Ming,Jiang, Jin-Fang,Liang, Wei-Hua,Li, Na,Li, Li,Chen, Yun-Zhao,Li, Feng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Background: A number of studies have identified a shared susceptibility locus in phospholipase C epsilon 1 (PLCE1) for esophageal squamous cell carcinoma (ESCC) and gastric cardia adenocarcinomas (GCA). However, the results of PLCE1 expression in esophageal and gastric cancer remain inconsistent and controversial. Moreover, the effects on clinicopathological features remain undetermined. This study aimed to provide a precise quantification of the association between PLCE1 expression and the risk of ESCC and GCA through meta-analysis. Materials and Methods: Eligible studies were identified from PubMed, Wanfang Data, ISI Web of Science, and the Chinese National Knowledge Infrastructure databases. Using RevMan5.2 software, pooled odds ratios (ORs) with 95% confidence intervals (CIs) were employed to assess the association of PLCE1 expression with clinicopathological features relative to ESCC or GCA. Results: Seven articles were identified, including 761 esophageal and gastric cancer cases and 457 controls. Overall, we determined that PLCE1 expression was associated with tumor progression in both esophageal cancers (pooled OR=5.93; 95%CI=3.86 to 9.11) and gastric cancers (pooled OR=9.73; 95%CI=6.46 to 14.7). Moreover, invasion depth (pooled OR=3.62; 95%CI=2.30 to 5.70) and lymph node metastasis (pooled OR=4.21; 95%CI=2.69 to 6.59) were linked with PLCE1 expression in gastric cancer. However, no significant associations were determined between PLCE1 overexpression and the histologic grade, invasion depth, and lymph node metastasis in esophageal cancer. Conclusions: Our metaanalysis results indicated that upregulated PLCE1 is significantly associated with an increased risk of tumor progression in ESCC and GCA. Therefore, PLCE1 expression can be appropriately regarded as a promising biomarker for ESCC and GCA patients.

Oil supplementation improved growth and diet digestibility in goats and sheep fed fattening diet

Su Chui Len Candyrine,Mohammad Faseleh Jahromi,Mahdi Ebrahimi,Wei Li Chen,Siamak Rezaei,Yong Meng Goh,Norhani Abdullah,Juan Boo Liang 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.4

Objective: This study evaluated the growth, digestibility and rumen fermentation between goats and sheep fed a fattening diet fortified with linseed oil. Methods: Twelve 3 to 4 months old male goats and sheep were randomly allocated into two dietary treatment groups in a 2 (species)×2 (oil levels) factorial experiment. The treatments were: i) goats fed basal diet, ii) goats fed oil-supplemented diet, iii) sheep fed basal diet, and iv) sheep fed oil-supplemented diet. Each treatment group consisted of six animals. Animals in the basal diet group were fed with 30% alfalfa hay and 70% concentrates at a rate equivalent to 4% of their body weight. For the oil treatment group, linseed oil was added at 4% level (w:w) to the concentrate portion of the basal diet. Growth performance of the animals was determined fortnightly. Digestibility study was conducted during the final week of the feeding trial before the animals were slaughtered to obtain rumen fluid for rumen fermentation characteristics study. Results: Sheep had higher (p<0.01) average daily weight gain (ADG) and better feed conversion ratio (FCR) than goats. Oil supplementation did not affect rumen fermentation in both species and improved ADG by about 29% and FCR by about 18% in both goats and sheep. The above enhancement is consistent with the higher dry matter and energy digestibility (p<0.05), as well as organic matter and neutral detergent fiber digestibility (p<0.01) in animals fed oil- supplemented diet. Sheep had higher total volatile fatty acid production and acetic acid proportion compared to goat. Conclusion: The findings of this study suggested that sheep performed better than goats when fed a fattening diet and oil supplementation at the inclusion rate of 4% provides a viable option to significantly enhance growth performance and FCR in fattening sheep and goats.

Honokiol Suppresses Renal Cancer Cells' Metastasis via Dual-Blocking Epithelial-Mesenchymal Transition and Cancer Stem Cell Properties through Modulating miR-141/ZEB2 Signaling

Li, Weidong,Wang, Qian,Su, Qiaozhen,Ma, Dandan,An, Chang,Ma, Lei,Liang, Hongfeng Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.5

Renal cell carcinoma (RCC) is associated with a high frequency of metastasis and only few therapies substantially prolong survival. Honokiol, isolated from Magnolia spp. bark, has been shown to exhibit pleiotropic anticancer effects in many cancer types. However, whether honokiol could suppress RCC metastasis has not been fully elucidated. In the present study, we found that honokiol suppressed renal cancer cells' metastasis via dual-blocking epithelial-mesenchymal transition (EMT) and cancer stem cell (CSC) properties. In addition, honokiol inhibited tumor growth in vivo. It was found that honokiol could upregulate miR-141, which targeted ZEB2 and modulated ZEB2 expression. Honokiol reversed EMT and suppressed CSC properties partly through the miR-141/ZEB2 axis. Our study suggested that honokiol may be a suitable therapeutic strategy for RCC treatment.

Cytogenetic and Genetic Mutation Features of de novo Acute Myeloid Leukemia in Elderly Chinese Patients

Su, Long,Li, Xian,Gao, Su-Jun,Yu, Ping,Liu, Xiao-Liang,Tan, Ye-Hui,Liu, Ying-Min Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Objectives: The present study aimed to examine the cytogenetic and genetic mutation features of acute myeloid leukemia (AML) in elderly Chinese patients. Methods: A retrospective analysis of cytogenetics and genetic mutations was performed in 113 cases (age range 50-82 years) with de novo AML. Results: The most frequent cytogenetic abnormality was t (15;17) (q22;q21), detected in 10.0% (n = 9) of successfully analyzed cases, followed by t (8;21) (q22;q22) in 8.89% (n = 8), and complex karyotypes in 5.56% (n = 5). Those with complex karyotypes included 4 cases (4.44%) of monosomal karyotypes. The frequencies of NPM1, FLT3-ITD, c-kit, and CEBPA mutations were 27.4% (31/113), 14.5% (16/110), 5.88% (6/102), and 23.3% (7/30), respectively. The complete remission rates of patients in low, intermediate, and high risk groups were 37.5%, 48.6%, and 33.3%, respectively (${\chi}^2$ = 0.704, P = 0.703) based on risk stratification. Conclusion: Cytogenetics and genetic mutations alone may not be sufficient to evaluate the prognoses of elderly AML patients. The search for a novel model that would enable a more comprehensive evaluation of this population is therefore imperative.

Honokiol Suppresses Renal Cancer Cells’ Metastasis via Dual-Blocking Epithelial-Mesenchymal Transition and Cancer Stem Cell Properties through Modulating miR-141/ZEB2 Signaling

Weidong Li,Qian Wang,Qiaozhen Su,Dandan Ma,Chang An,Lei Ma,Hongfeng Liang 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.5

Renal cell carcinoma (RCC) is associated with a high frequency of metastasis and only few therapies substantially prolong survival. Honokiol, isolated from Magnolia spp. bark, has been shown to exhibit pleiotropic anticancer effects in many cancer types. However, whether honokiol could suppress RCC metastasis has not been fully elucidated. In the present study, we found that honokiol suppressed renal cancer cells’ metastasis via dual-blocking epithelial-mesenchymal transition (EMT) and cancer stem cell (CSC) properties. In addition, honokiol inhibited tumor growth in vivo. It was found that hono-kiol could up-regulate miR-141, which targeted ZEB2 and modulated ZEB2 expression. Honokiol reversed EMT and suppressed CSC properties partly through the miR-141/ZEB2 axis. Our study suggested that honokiol may be a suitable therapeutic strategy for RCC treatment.

Anti-hyperuricemic and nephroprotective effects of Rhizoma Dioscoreae septemlobae extracts and its main component dioscin via regulation of mOAT1, mURAT1 and mOCT2 in hypertensive mice

Junxia Su,Yu-Hui Wei,Minglong Liu,Tianxi Liu,Jianhua Li,Yuanchun Ji,Jianping Liang 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.10

Rhizoma Dioscoreae septemlobae (RDSE) hasbeen widely used for the treatment of hyperuricemia inChina. However, the therapeutic mechanism has beenunknown. This study investigated the antihyperuricemicmechanisms of the extracts obtained from RDSE and itsmain component dioscin (DIS) in hyperuricemic mice. Hyperuricemic mice were induced by potassium oxonate(250 mg/kg). RDSE or DIS was orally administered tohyperuricemic mice at dosages of 319.22, 638.43,1276.86 mg/kg/day for 10 days, respectively. Uric acid orcreatinine in serum and urine was determined by HPLC orHPLC–MS/MS, respectively. The xanthine oxidase (XO)activities in mice liver were examined in vitro. Proteinlevels of organic anion transporter 1 (mOAT1), uratetransporter 1 (mURAT1) and organic cation transporter 2(mOCT2) in the kidney were analyzed by western blotting. The results indicated that uric acid and creatinine in serumwere significantly increased by potassium oxonate, ascompared to that of control mice. Compared saline-treatedgroup, after RDSE treatment in the high and middle dose,the expression of mOAT1 increased 47.98 and 54.48 %,respectively, which accompanied with the decreasedexpression of mURAT1 (47.63 %) in high dose. After DIS treatment in high, middle and low dose, the expression ofmOAT1 increased 23.93, 32.80 and 25.28 % compared tosaline-treated group, respectively, which accompanied withthe decreased expression of mURAT1 (51.07, 51.42 and51.35 %). However, RDSE and DIS displayed a weak XOinhibition activity compared with allopurinol. Therefore,RDSE and DIS processed uricosuric and nephroprotectiveactions by regulation of mOAT1, mURAT1 and mOCT2.

MnO2/carbon nanotube-embedded carbon nanofibers as core–shell cables for high performing asymmetric flexible supercapacitors

Cheng-Liang Huang,Li-Ming Chiang,Chien-An Su,YUAN-YAO LI 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.103 No.-

Supercapacitors (SCs) are important devices for energy storage because they provide a higher power densityas compared to that of batteries. However, SCs with high energy density and good flexibility are stillunder development. In this study, we fabricated MnO2-coated Carbon nanotube (CNT)-embedded carbonnanofiber (CNF) (referred to as MnO2/CNF-CNT) core–shell cables to investigate pseudo-capacitators,while activated CNF-CNTs obtained by CO2 activation was used for Electrical double-layer capacitors(EDLCs). Owing to the unique structure of the materials and high electrical conductivity of the CNTson the CNFs, a specific capacitance of 483.5 F/g was achieved using MnO2/CNF-CNT mat with 1 MNa2SO4 aqueous electrolyte at 0.5 A/g. A flexible MnO2/CNF-CNT supercapacitor was assembled, whichwas binder-free, electrically conductive media-free, and current collector-free. Further an asymmetricsupercapacitor was assembled with the MnO2/CNF-CNT mat and activated CNF-CNT mat in an ionic liquid(1-ethyl-3-methylimidazolium bis (trifluoromethylsulfonyl) imide) electrolyte. The maximum specificcapacitance of, energy density of and power density of 94.25 F/g, 209.4 Wh/kg and 1000 W/kg can beachieved with the asymmetric supercapacitor at 0.5 A/g and 4.0 V. We believe that these materials havesignificant potential for use in light-weight and flexible SCs.

A Light-weighted Data Collection Method for DNS Simulation on the Cyber Range

( Shuang Li ),( Shasha Du ),( Wenfeng Huang ),( Siyu Liang ),( Jinxi Deng ),( Le Wang ),( Huiwu Huang ),( Xinhai Liao ),( Shen Su ) 한국인터넷정보학회 2020 KSII Transactions on Internet and Information Syst Vol.14 No.8

The method of DNS data collection is one of the most important parts of DNS simulation. DNS data contains a lot of information. When it comes to analyzing the DNS security issues by simulation on the cyber range with customized features, we only need some of them, such as IP address, domain name information, etc. Therefore, the data we need are supposed to be light-weighted and easy to manipulate. Many researchers have designed different schemes to obtain their datasets, such as LDplayer and Thales system. However, existing solutions consume excessive computational resources, which are not necessary for DNS security simulation. In this paper, we propose a light-weighted active data collection method to prepare the datasets for DNS simulation on cyber range. We evaluate the performance of the method and prove that it can collect DNS data in a short time and store the collected data at a lower storage cost. In addition, we give two examples to illustrate how our method can be used in a variety of applications.

Associations of HSP90AA2 gene polymorphisms with disease susceptibility, glucocorticoids efficacy and health-related quality of life in Chinese systemic lupus erythematosus patients

Man Zhang,Su‑Su Li,Qiao‑Mei Xie,Jian‑Hua Xu,Xiu‑Xiu Sun,Fa‑Ming Pan,Sheng‑Qian Xu,Sheng‑Xiu Liu,Jin‑Hui Tao,Shuang Liu,Jing Cai,Pei‑Ling Chen,Long Qian,Chun‑Huai Wang,Chun‑Mei Liang,Hai‑Liang Huang,Ha 한국유전학회 2018 Genes & Genomics Vol.40 No.10

Although the current glucocorticoids (GCs) treatment for systemic lupus erythematosus (SLE) is effective to a certain extent, the difference in therapeutic effect between patients is still a widespread problem. Some patients can have repeated attacks that greatly diminish their quality of life. This study was conducted to investigate the relationship between HSP90AA2 polymorphisms and disease susceptibility, GCs efficacy and health-related quality of life (HRQoL) in Chinese SLE patients. A case–control study was performed in 470 SLE patients and 470 normal controls. Then, 444 patients in the case group were followed up for 12 weeks to observe efficacy of GCs and improvement of HRQoL. Two single nucleotide polymorphisms (SNPs) of HSP90AA2 were selected for genotyping: rs1826330 and rs6484340. HRQoL was assessed using the SF-36 questionnaire. The minor T allele of rs1826330 and the TT haplotype formed by rs1826330 and rs6484340 showed associations with decreased SLE risk (T allele: PBH = 0.022; TT haplotype: PBH = 0.033). A significant association between rs6484340 and improvement of HRQoL was revealed in the follow-up study. Five subscales of SF-36 were appeared to be influenced by rs6484340: total score of SF-36 (additive model: PBH = 0.026), physical function (additive model: PBH = 0.026), rolephysical (recessive model: PBH = 0.041), mental health (dominant model: PBH = 0.047), and physical component summary (additive model: PBH = 0.026). No statistical significance was found between HSP90AA2 gene polymorphisms and GCs efficacy. These results revealed a genetic association between HSP90AA2 and SLE. Remarkably, HSP90AA2 has an impact on the improvement of HRQoL in Chinese population with SLE.