http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
BMB Reports : Apoptosis inhibitor 5 increases metastasis via Erk-mediated MMP expression
( Kwon Ho Song ),( Seok Ho Kim ),( Kyung Hee Noh ),( Hyun Cheol Bae ),( Jin Hee Kim ),( Hyo Jung Lee ),( Jinhoi Song ),( Tae Heung Kang ),( Dong Wan Kim ),( Se Jin Oh ),( Ju Hong Jeon ),( Tae Woo Kim 생화학분자생물학회(구 한국생화학분자생물학회) 2015 BMB Reports Vol.48 No.6
Apoptosis inhibitor 5 (API5) has recently been identified as a tumor metastasis-regulating gene in cervical cancer cells. However, the precise mechanism of action for API5 is poorly understood. Here, we show that API5 increases the metastatic capacity of cervical cancer cells in vitro and in vivo via up-regulation of MMP-9. Interestingly, API5-mediated metastasis was strongly dependent on the Erk signaling pathway. Conversely, knock-down of API5 via siRNA technology decreased the level of phospho-Erk, the activity of the MMPs, in vitro invasion, and in vivo pulmonary metastasis. Moreover, the Erk-mediated metastatic action was abolished by the mutation of leucine into arginine within the heptad leucine repeat region, which affects protein-protein interactions. Thus, API5 increases the metastatic capacity of tumor cells by up-regulating MMP levels via activation of the Erk signaling pathway. [BMB Reports 2015; 48(6): 330-335]
통증자가조절법과 병용한 늑간신경차단이 개흉술후 통증과 흡기용적에 미치는 영향
송선옥,김세연,김흥대,권오득,정태은 대한마취과학회 1998 Korean Journal of Anesthesiology Vol.34 No.6
Background : This study was performed to evaluate the effects of intercostal nerve block added in intravenous patient-controlled analgesia(IV-PCA; PCA) on pain, pulmonary function and the movement of the ipsilateral arm after a thoracotomy. Methods : Forty five patients undergoing elective thoracotomy were randomly allocated into one of three groups. The groups were divided as follows: PCA, ICB-PCA(PCA and intercostal nerve blocks by direct injection of 5 ml of 0.2% bupivacaine into the intercostal spaces of two upper and two lower segments around the surgical incision) and IM groups. For the PCA, the patients that received PCA, were administered IV bolus of 0.1 mg/kg of nalbuphine followed by PCA with 0.1% nalbuphine(basal rate 0.5 ml/hr, bolus dose 1 mg and lockout interval 8 minutes). In each group, VAS score, the inspiratory capacity and the movement of the ipsilateral arm were checked postoperatively at 6, 24, 48 and 72 hours. Results : Inspiratory capacity was decreased less in ICB-PCA group(P<0.05) at6 hour, but after 24 hour, there were no differences between the groups. The analgesic effect was significantly better in ICB-PCA group as compared to the PCA or IM groups(P<0.05). Furthermore, arm motion limitation after operation was the least in ICB-PCA group(P<0.01). Conclusions : Intraoperative intercostal nerve blocks added in PCA has a transient improvement of pulmonary function, and also provide better analgesia and improved ipsilateral arm motion after a thoracotomy than in PCA or IM analgesia. The authors recommend adding intercostal nerve block for patients undergoing thoracotomy who receive IV-PCA. (Korean J Anesthesiol 1998; 34: 1247∼1253)
THE EFFECT OF ACUTE TOLUENE EXPOSURE ON HIPPOCAMPAL NEUROGENESIS IN ADULT RATS
Heung-Sik Seo,Young-Su Yang,Jinsoo Lee,Min-Sung Kang,Soonjin Kwon,Seong-Jin Choi,Soo-Nam Kim,Byeong-Chan Lee,Hyo-Seon Yang,Young-Ah Han,Hee-Jin Yu,Jae-Hwan Kim,Jin-Hee Lee,Jeong-Ah Song,Kyung Jin Jung 한국환경독성학회 2009 한국독성학회 심포지움 및 학술발표회 Vol.2009 No.11
ACUTE INHALATION TOXICITY STUDY WITH TOLUENE IN RATS
Heung-Sik Seo,Young-Su Yang,Jinsoo Lee,Min-Sung Kang,Soonjin Kwon,Seong-Jin Choi,Soo-Nam Kim,Byeong-Chan Lee,Hyo-Seon Yang,Yong-Ah Han,Hee-Jin Yu,Jae-Hwan Kim,Jin-Hee Lee,Jeong-Ah Song,Kyung Jin Jung 한국환경독성학회 2009 한국독성학회 심포지움 및 학술발표회 Vol.2009 No.11
Song, Suk-Heung,Kim, Kibeom,Choi, Sung-Eun,Han, Sangkwon,Lee, Ho-Suk,Kwon, Sunghoon,Park, Wook Optical Society of America 2014 Optics letters Vol.39 No.17
<P>This article presents free-floating three-dimensional (3D) microstructure fabrication in a microfluidic channel using direct fine-tuned grayscale image lithography. The image is designed as a freeform shape and is composed of gray shades as light-absorbing features. Gray shade levels are modulated through multiple reflections of light in a digital micromirror device (DMD) to produce different height formations. Whereas conventional photolithography has several limitations in producing grayscale colors on photomask features, our method focuses on a maskless, single-shot process for fabrication of freeform 3D micro-scale shapes. The fine-tuned gray image is designed using an 8-bit grayscale color; thus, each pixel is capable of displaying 256 gray shades. The pattern of the UV light reflecting on the DMD is transferred to a photocurable resin flowing through a microfluidic channel. Here, we demonstrate diverse free-floating 3D microstructure fabrication using fine-tuned grayscale image lithography. Additionally, we produce polymeric microstructures with locally embedded gray encoding patterns, such as grayscale-encoded microtags. This functional microstructure can be applied to a biophysical detection system combined with 3D microstructures. This method would be suitable for fabricating 3D microstructures that have a specific morphology to be used for particular biological or medical applications.</P>
Kwon, Byung-Su,Jung, Heung-Su,Song, Min-Sun,Cho, Kyung Sook,Kim, Sung-Chun,Kimm, Kuchan,Jeong, Jin Sook,Kim, In-Hoo,Lee, Seong-Wook Elsevier 2005 Molecular therapy Vol.12 No.5
<P><B>Abstract</B></P><P>In this study, we describe a novel approach to human cancer therapy that is based upon <I>trans</I>-splicing ribozyme-mediated replacement of cancer-specific RNAs with new transcripts that exert therapeutic activities. We have developed a specific ribozyme that can reprogram human telomerase reverse transcriptase (hTERT) RNA to induce transgene activity selectively in cancer cells that express the RNA. The ribozyme-mediated triggering of the transgene expression was accomplished via a high-fidelity <I>trans</I>-splicing reaction with the targeted residue in the hTERT-expressing cells. The ribozyme also induced cytotoxic activity in various hTERT-expressing cancer cells, hence selectively retarding the growth of those cells. Efficient and specific cell regression was also detected with ganciclovir (GCV) treatment only in hTERT-positive cancer cells, which were established to express stably the specific ribozyme that contains the herpes simplex virus thymidine kinase (HSV-<I>tk</I>) gene. Tissue-specific expression of the ribozyme could further augment the target specificity of the ribozyme. Importantly, we observed efficient regression of tumors with GCV treatment in mice that had been inoculated subcutaneously with hTERT-positive cancer cells that stably expressed the specific ribozyme that contains HSV-<I>tk</I>. These results suggest that the hTERT RNA-targeting <I>trans</I>-splicing ribozyme could be a powerful agent for tumor-targeted specific gene therapy.</P>
Papain-like protease (PLpro) inhibitory effects of cinnamic amides from Tribulus terrestris fruits.
Song, Yeong Hun,Kim, Dae Wook,Curtis-Long, Marcus John,Yuk, Heung Joo,Wang, Yan,Zhuang, Ningning,Lee, Kon Ho,Jeon, Kwon Seok,Park, Ki Hun Pharmaceutical Society of Japan 2014 Biological & pharmaceutical bulletin Vol.37 No.6
<P>Tribulus terrestris fruits are well known for their usage in pharmaceutical preparations and food supplements. The methanol extract of T. terrestris fruits showed potent inhibition against the papain-like protease (PLpro), an essential proteolylic enzyme for protection to pathogenic virus and bacteria. Subsequent bioactivity-guided fractionation of this extract led to six cinnamic amides (1-6) and ferulic acid (7). Compound 6 emerged as new compound possessing the very rare carbinolamide motif. These compounds (1-7) were evaluated for severe acute respiratory syndrome coronavirus (SARS-CoV) PLpro inhibitory activity to identify their potencies and kinetic behavior. Compounds (1-6) displayed significant inhibitory activity with IC50 values in the range 15.8-70.1??M. The new cinnamic amide 6 was found to be most potent inhibitor with an IC50 of 15.8??M. In kinetic studies, all inhibitors exhibited mixed type inhibition. Furthermore, the most active PLpro inhibitors (1-6) were proven to be present in the native fruits in high quantities by HPLC chromatogram and liquid chromatography with diode array detection and electrospray ionization mass spectrometry (LC-DAD-ESI/MS).</P>