Host range of Cybocephalus flavocapitis and Cybocephalus nipponicus, two potential biological control agents for the cycad aulacaspis scale, Aulacaspis yasumatsui

Sing-Ying Song,Ching-Wen Tan,Shaw-Yhi Hwang 한국응용곤충학회 2012 Journal of Asia-Pacific Entomology Vol.15 No.4

Host-range and host-specificity tests were performed with Cybocephalus flavocapitis T. R. Smith (Coleoptera:Cybocephalidae) and Cybocephalus nipponicus Endrödy-Younga (Coleoptera: Cybocephalidae), two biological control candidates against the invasive cycad aulacaspis scale, Aulacaspis yasumatsui Takagi (Hemiptera: Diaspididae). Seventeen native scale species plus the invasive A. yasumatsui scale were tested in growth chambers using nochoice tests and hosts suitable for each of the two predatory beetles. The results revealed that the two Cybocephalus beetles, one imported species from Thailand and one native species, both fed on relatively similar scale prey species. Additionally, the adult beetles of these two species oviposited only on Diaspididae scales. The results showed that both Cybocephalus beetles may share a similar host niche in Taiwan

Study of Pulse Generation Technique for Serial dual Electrode Detection of Amino Acids and Proteins in Flow Injection Analysis

Ying-Sing Fung,Song-Ying Mo 한국분석과학회 1995 분석과학 Vol.8 No.4

Study of Pulse Generation Technique for Serial dual Electrode Detection of Amino Acids and Proteins in Flow Injection Analysis

Fung, Ying-Sing,Mo, Song-Ying 한국분석과학회 1995 분석과학 Vol.8 No.4

A new analytical procedure using a serial dual electrode detector was developed for the analysis of amino acids and proteins. Bromine was generated at the upstream electrode and detected by the downstream electrode. The presence of amino acids and proteins was shown to lower the downstream current but with no apparent effect on the upstream current. This indirect mode of detection can be applied to the determination of amino acids and proteins which are electrochemically inactive or too large to be accessible to the electrode surface for electron exchange. The method is shown capable to determine various amino acids (cystine, tyrosine, lysine, tryptophan, glycine, methionine and arginine) and proteins (cytochrome c, hemoglobin, HAS, a-Amylase, Conalbumin I, Catalase and Myglobin) with linear working range for amino acids between $10^{-6}$ to $10^{-3}M$ and total proteins between $10^{-7}$ to $10^{-3}M$. The method has been applied for the analysis of amino acids and total protein in food using Flow Injection Analysis with results obtained comparable to those using the traditional analytical procedure. Use of pulse generation technique was shown to produce a more stable flow injection analysis peaks for repetitive determination than the use of conventional constant current method which showed increase of the background current after determination over 200 minutes. The pulse method was found to give stable baseline even after 400 minutes. Thus, the method is shown able to provide a suitable analytical procedure for automatic analysis of amino acids and proteins in food by flow injection analysis.

Discovery of Plant Antagonists of Insect Juvenile Hormone

Seok-Hee Lee,Ying Fang,Saes-Byeol An,Doo-Sang Park,Hyuk-Hwan Song,Sei-Ryang Oh,Soo-Young Kim,Seonghyun Kim,Namjung Kim,Hyun-Woo Oh,Yeon Ho Je,Sang Woon Shin 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10

Insects impact human health through vector-borne diseases and cause major economic loses through damaging crops and stored agricultural products. Insect-specific growth regulators (IGR) represent attractive control agents because of their safety to the environment and humans. Here, we report identification of plant compounds that are antagonists of the insect-specific juvenile hormone (PJHANs), using the yeast two hybrid system transformed with the mosquito JH receptor as a reporter assay. We show that these compounds act by inhibiting larval growth and reproduction in mosquitoes. We also demonstrate that PJHANs affect the JH receptor, Methoprene-tolerant (Met), by disrupting its complex with CYCLE, formation of which is required for mediating JH action. We isolated five diterpene secondary metabolites with JH antagonist activity from two plants, Lindera erythrocarpa and Solidago serotina. They are effective in causing mortality of mosquito larvae at relatively low LD50 values. Two of these diterprenes affect Met function, leading to reduction in expression of Met target genes and causing retardation of follicle development in mosquito ovaries. Developing potent compounds counteracting JHaction (JH antagonists) would find a wider range of control applications. However, so far such JH antagonists have not been developed. Here, we report the discovery of potent JH antagonists in plants, which represents an innate resistance mechanism of plants against insect herbivores. These newly discovered plant JH antagonist compounds could be used as the starting material for developing novel insecticides.

VDUP1 Is Required for the Development of Natural Killer Cells

Lee, Kee Nyung,Kang, Hyung-Sik,Jeon, Jun-Ho,Kim, Eun-Mi,Yoon, Suk-Ran,Song, Hyunkeun,Lyu, Chil-Youl,Piao, Zheng-Hao,Kim, Sun-Uk,Han, Ying-Hao,Song, Su-Sung,Lee, Young-Ho,Song, Kyu-Sang,Kim, Yong-Man,Y Elsevier 2005 Immunity Vol.22 No.2

<P><B>Summary</B></P><P>Vitamin D<SUB>3</SUB> upregulated protein 1 (VDUP1) is a stress-response gene that is upregulated by 1,25(OH)<SUB>2</SUB>D<SUB>3</SUB> in tumor cells. The in vivo roles of VDUP1 were investigated by producing mice lacking VDUP1 (VDUP1<SUP>−/−</SUP> mice). VDUP1<SUP>−/−</SUP> mice showed minimal changes in the development of T and B cells, but there was a profound reduction in the numbers of natural killer (NK) cells. As well, these mice showed decreased NK activity. In the VDUP1<SUP>−/−</SUP> mice, the expression of CD122 was reduced, demonstrating that VDUP1 is required for CD122 expression and NK maturation. In addition, severe lymphoid hyperplasia in the small intestine was observed in VDUP1<SUP>−/−</SUP> mice. Taken together, these results suggest that VDUP1 is a critical factor for the development and function of NK cells in vivo.</P>

중국관광객의 면세점 바오마이 결정요인에 대한 실증연구

임성훈(Sung-Hoon Lim),고숭(Song Gao),진가영(Jia-Ying Chen) 한국무역학회 2020 貿易學會誌 Vol.45 No.5

국내 팔로워십 연구동향 분석

박상욱(Sang wook, Park),강준영(Jun Ying, Jiang),전다영(Da young, Jeon),송영수(Young soo, Song) 한국기업교육학회 2018 기업교육과인재연구 Vol.20 No.3

본 연구는 2000년부터 2017년까지 KCI 등재지와 등재후보지에 게재된 팔로워십 연구 논문분석을 통해 학문적, 실제적 영역에서 시사점을 제공하는데 그 목적이 있다. 이를 위해 총 128편의 팔로워십 연구 논문에 대해 연구대상, 연구주제, 연구방법을 연도별 논문수와 비율을 비교하여 분석하였으며, 주요 결과는 다음과 같다. 첫째, 연구대상 중 국내기업을 대상으로 진행된 연구가 약 50%로 가장 많았으며 팔로워십 역량 개발, 유형 연구 등 팔로워십 연구 분야를 확장시키고 흐름을 주도했던 것으로 나타났다. 둘째, 연구주제 중 리더십에 대한 팔로워십 영향력에 대한 연구가 집중되었다. 즉, 팔로워십과 리더십을 독립적으로 인식하는 경우(Leadership Vs. Followership), 리더십과 상호 관계적으로 인식하는 경우(Leadership & Followership), 리더십 내에 포함된 개념으로 인식하는 경우(Followership in Leadership) 중에서 세 번째 경우의 연구가 압도적이었다. 이는 리더의 역할을 중요시하고 리더 중심적 연구가 주로 이루지고 있음을 알 수 있다. 셋째, 연구방법 분석에서는 양적연구방법을 활용한 연구가 큰 비중을 차지하고 있는 것으로 나타났다. 이상의 연구 결과를 바탕으로 향후 국내 팔로워십 연구에 기여하고 HRD영역에서 다루어져야할 팔로워십 연구 방향에 대한 시사점을 제공하고자 한다. The purpose of this research is to provide academic and practical viewpoints on followership by analyzing registered publications on the Korea Citation Index (KCI) and other journals between 2000 and 2017. For this, a total of 128 research publications were reviewed on research subjects, research topics and research methods. The publications were classified by the number of and ratio of papers published per year. The main findings are given below. First, the most commonly studied research subjects (about 50%) were domestic companies. Such studies on Korean companies were found to be leading the trends, as well as playing a role in expanding the research field of followership (including study into follower competence and types of followership). Second, research topics focused mostly on the influence of followership on leadership. This was seen in three forms: followership independent of leadership (leadership vs. followership), followership related to leadership (leadership and followership), and followership as a concept included in leadership (followership in leadership). An overwhelming number of studies has been done in the last form, implying that the role of a leader is considered crucial, and that leader-centered research is what is mainly conducted. Third, with regard to the research method, quantitative research method was most favored. Based on such results, we wish to contribute to the expansion and growth of research on followership, as well as present practical and theoretical implications for the direction of followership studies in the field of human resources development.

Suitability of Fourier Transform Infrared Spectroscopy as a screening method for the production of useful mutant lines in Panax ginseng

Javzandulam Ulziisaikhan,Jun-Ying Zhang,Hong-Yu Li,Hyeon-Jin Sun,Somi Kim,Sung-Jun Song,Hyo-Yeon Lee 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Panax ginseng C.A Meyer is commonly used in Asian traditional medicine to treat a variety of diseases. Ginsenosides are glycosylated triterpenes, referred to saponins, have been especially noted as active compounds contributing to the various efficacy of ginseng. In this study, we are trying to select high saponin content of ginseng lines from the gamma irradiated adventitious roots. Recently, we have generated several mutant ginseng lines improving ginseniside content by gamma radiation. The mutant lines were selected by phenotypes and ginsenoside content (HPLC analysis) of the irradiated adventitious root lines. However, the ginsenoside content of the mutant lines was not sufficient for commercial use and the selection method was not suitable for large scale of mutant line selection. In this study, we are testing Fourier transfer infrared spectroscopy (FT-IR) as a new selection method of mutant lines in Panax ginseng. About 5,000 pieces of Panax ginseng adventitious roots were exposed to gamma radiation (60Co). Irradiation dosages were 0, 25, 50 and 70Gy. Survival rate of the irradiated samples was evaluated by counting the number of survival main roots after 5 weeks culture in the solid MS medium with NAA, IAA and 5% sucrose. In present, we are collecting the survived adventitious root lines (about 900 lines) from the gamma irradiated ginseng roots for FT-IR and HPLC analysis. After analysis of FT-IR and HPLC, we will assess the suitability of the FT-IR as a screening method for the preparation of mutant lines in ginseng.

Transcriptome Analysis of the Sacbrood Virus-infected Asian Honeybee, Apis cerana.

Seok Hee Lee,Sang Woon Shin,Qin Liu,Song Eun Kim,Saes Byeol An,Fang Ying,Jae Young Choi,Woo Jin Kim,Yeon Ho Je 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10

Sacbrood virus (SBV) is one of the most fatal pathogens against Asian honeybee, Apis cerana. This virus cause failure of the insect larvae to pupate and death of the adult insects. This study has analyzed the host genes affected by viral infection, by comparing the expression level of host transcripts infected with or without SBV. As a first step, we sequenced the cDNA libraries of Asian honeybee by using illumina RNA sequencing. The sequences were de novo assembled to acquire honeybee transcriptome sequences. The transcriptome was annotated by the sequence comparison to known protein sequences by BLASTX and evolutionary genealogy of genes: Non-supervised Orthologous Groups (eggNOG) database with functional categories and description. By mapping the RNA-seq data to de novo assembled transcripts, we characterized the differentially expressed transcripts between SBV-infected and non-infected Asian honeybee.

Primary Involvement of NADPH Oxidase 4 in Hypoxia-Induced Generation of Reactive Oxygen Species in Adipose-Derived Stem Cells

Kim, Ji Hye,Song, Seung-Yong,Park, Sang Gyu,Song, Sun U.,Xia, Ying,Sung, Jong-Hyuk Mary Ann Liebert 2012 STEM CELLS AND DEVELOPMENT Vol.21 No.12

<P>We have previously demonstrated that hypoxia stimulates adipose-derived stem cells (ASCs) through the generation of reactive oxygen species (ROS). However, the precise mechanism involved in the ROS generation by ASCs is not well understood. We sought to investigate in this work: (1) which subtype of NADPH oxidase (Nox) is primarily expressed in ASCs; (2) where Nox4 is localized in ASCs; and (3) whether silencing of Nox4 attenuates hypoxia-enhanced function of ASC. We used 2',7'-dichlorofluorescin diacetate (DCF-DA) as an indicator of ROS generation and found that the fluorescence intensity of DCF-DA was significantly increased after hypoxia exposure (2% oxygen). In addition, hypoxia enhanced the proliferation and migration of ASCs and upregulated the mRNA expression of Oct4 and Rex1. Quantitative analysis of mRNA expression of Nox family in ASCs demonstrated that Nox4 is primarily expressed in ASCs, while immunofluorescence assay showed that Nox4 is mainly localized in the perinuclear region and overlaps with Mitotracker, a mitochondria marker. Silencing of Nox4 by siRNA treatment downregulated the RNA and protein expression of Nox4, which significantly reduced the ROS generation under hypoxia. In addition, Nox4 silencing significantly reduced the proliferation and migration of ASCs and downregulated the mRNA expression of Oct4 and Rex1. Phosphorylation of platelet-derived growth factor receptor-β, AKT, and ERK1/2 also diminished following Nox4 silencing. In a nutshell, these results suggest that Nox4 is primarily expressed in ASCs and plays a pivotal role in the hypoxia-enhanced stimulation of ASCs.</P>