Genome-wide screening antifungal genes in <i>Streptomyces griseus</i> S4-7, a Fusarium wilt disease suppressive microbial agent

Hong, Sung Won,Kim, Da-Ran,Kwon, Youn Sang,Kwak, Youn-Sig Published by Elsevier/North Holland on behalf of t 2019 FEMS microbiology letters Vol.366 No.12

<P> <I>Streptomyces</I> is a widely studied bacterial genus, particularly with regard to secondary metabolites and antibiotics production. <I>Streptomyces griseus</I> S4-7 was isolated from a strawberry Fusarium wilt disease suppressive soil, and its biological control ability has been well established. However, the antifungal mechanism of strain S4-7 is not yet fully understood at the molecular and biochemical level. Therefore, in this study we created a random mutant library for strain S4-7 with the Tn5 transposon element to investigate antifungal traits on a genome-wide scale. In total 4646 individual mutant strains were created and 13 mutants were selected based on loss of antifungal activity. The knockout genes were identified as electron transfer oxidoreductase (eto),sigma factor-70(sig70) and nrps by Inverse PCR (I-PCR). eto regulates the <I>geranylgeranyl</I> reductase gene, which is involved in terpenoid-quinone biosynthesis, an important factor in cell fitness. In the <I>△eto</I> strain, expression of <I>wbl,</I> a master regulator of the production of secondary metabolites, was significantly reduced. sig70 is responsible for the cell differentiation sensing mechanism in genus <I>Streptomyces. △nrps</I> showed decreased production of hybrid peptide-polyketide siderophores. These results suggest that <I>S. griseus</I> S4-7 may have various antifungal mechanisms, and each mechanism is essential to maximal antifungal activity. <P>

D.H.Lawrence의 생명주의 Ⅰ

황규식(Hwang, Kew·Sig) 부산대학교 인문학연구소 1985 코기토 Vol.27 No.-

Antitumor activity of spinasterol isolated from Pueraria roots

Gook-Che Jeon,Myoung-Soon Park,윤도영,C hul-Ho Shin,Hong-Sig Sin,엄수종 생화학분자생물학회 2005 Experimental and molecular medicine Vol.37 No.2

We purified phytoestrogens from Pueraria root (Pueraria mirifica from Thailand and Pueraria lobata from Korea), which is used as a rejuvenat-ing folk medicine in Thailand and China. Dried, powdered plant material was extracted with 100% ethanol and further separated by concentration, filtration, and thin layer silica gel chromatogra-phy. Using the fractions obtained during separa-tion, we first investigated their cytotoxicity in se-veral cancer cel lines from various tissues. The ethanol-extracted components (PE1, PE4) had sig-nificant antiproliferative efects on breast cancer cel lines, including MCF-7, ZR-75-1, MDA-MB-231, SK-BR-3, and Hs578T. Second, we compared flavonoids, sterols, and coumarins from Pueraria root. The known compounds were not as efec-tive, and occured in a diferent polarity region on HPLC. Third, further separation resulted in the isolation of eight diferent components (Sub PE-A to -H). One of these, PE-D, affected the growth of some breast cancer cel lines (MCF-7, MDA- MB-231) in a dose- and time-dependent maner, as wel as the growth of ovarian (2774) and cervical cancer cels (HeLa). Finaly, a transfec-tion assay showed that this component had an estrogenic effect similar to 17βactivates both estrogen receptor α (ERα) and ERβ. The NMR analysis determined that spin-asterol (stigmasta-7, 22-dien-3beta-ol) is an active cytotoxic component of Pueraria root.

몰리브덴(V)와 시트릭액시드계 착물합성과 그 성질

최식영,이재덕 安東大學 1989 安東大學 論文集 Vol.11 No.1

A new series of dioxo-di-μ-oxo-dimolybdate(v), ◁화학식 삽입▷(원문을 참조하세요), has been prepared by reaction of pyridinum oxoisothiooyantomolybdate(v) with citric acid derivatives containing amine carboxyl groups. The properties and possible molecular structure of these complexes were discussed by elemental analysis, spectroscopic studies and magnetic susceptibility measurements. The infrared spectra of these complexes all show two strong Mo=O2 stretching bands in the 900~980cm-1, MoO2Mo very prominent strectching bands at around 410~425 and 735~750cm^(-1) to symmetrical and asymmetrical O-bridge stretching, a coordinated coo^(-) asymmetrical band in the 1585~1640cm^(-1). Also, d-d transition of molybdenyl complexes corresponding to ^(2)B_(2)-^(2)B_(1) occured in the 24,800~28,000cm^(-1) region, charge transfer transition corresponding to ligand-to-molybdenum in the 32,500~33,800, 42,000 ~47,500cm^(-1) region. The complexes synthesized were yellow or orange and diamagnetic.

몰리브덴 (V)와 니트리로아세트산계 착물 합성과 그 성질

최식영 安東大學 1987 安東大學 論文集 Vol.9 No.1

A new series of dioxo-di-μ-oxo-dimolybdate (V), ◁화확식 삽입▷(원문을 참조하세요), has been prepared by reaction of pyridinum oxoisothiocyantomolybdate (V) with nitrilotriacetic acid deivatives containing amine carboxyl groups. The properties and possible molecular structure of these complexes were discussed by elemental analysis, spectroscopic studies and magnetic susceptibility measurements. The infrared spectra of these complexes all show two strong Mo=Ot stretching modes in the 900~980cm^(-1), MoO2 Mo very prominent stretching bands at around 410~425 and 735~750cm^(-1) to symmetrical and antisymmetrical O-bridge stretching, a coordinated COO- antisym-metrical band in the 1585~1640cm^(-1). Also, d-d transition of molybdenyl complexes corresponding to ^(2)B_(2)→^(2)B_(1) occured in the 24,800~28,000cm-1 region, charge transfer transition corresponding to ligand-to-molybdenum in the 32,500~33, 800, 42,000~475,00cm^(-1) region. The complexes synthesized were yellow and diamagnetic.

몰리브덴(V)와 1,2-Diaminopropane-N,N,N´,N´-tetraacetic acid계 착물 합성과 그 성질

최식영 安東大學 1988 安東大學 論文集 Vol.10 No.1

A new series of dioxo-di-μ-oxo-dimolybdate (V), ◁화학식 삽입▷(원문을 참조하세요), has been prepared by reaction of pyridinum oxoisothiocyanato mdolybdate (V) with 1, 2-Diaminopropane-N, N, N`, N`-tetracetate derivatives containing amine carboxyl groups. The properties and possible molecular structure of these complexes were discussed by elemental analysis, spectroscopic studies and magnetic susceptibility measurements. The infrared spectra of these complexes all show two strong Mo=Ot stretching modes in the 900~980cm^(-1), MoO_(2)Mo very prominent stretching bands at around 410~425 and 735~750cm^(-1) to symmetrical and asymmetrical O-bridge stretching, a coordinated coo-asymmetrical band in the 1585~1640cm^(-1). The complexes were yellow and diamagnetic.

Relationship between Spinopelvic Parameters and Hip Function in Patients with Femoroacetabular Impingement at Diagnosis: A Cross-Sectional Study

Bernardo Aguilera-Bohórquez,Pablo Corea,Cristina Sigüenza,Jochen Gerstner-Saucedo,Alvaro Carvajal,Erika Cantor 대한고관절학회 2023 Hip and Pelvis Vol.35 No.1

Purpose: The aim of this study was to determine correlation between the spinopelvic parameters in sitting and standing positions (sacral slope [SS], lumbar lordosis [LL], spinopelvic tilt [SPT], pelvic incidence [PI], and pelvic femoral angle [PFA]), with hip function assessed using the modified Harris hip scores (mHHs) in patients with symptomatic femoroacetabular impingement (FAI) at diagnosis. Materials and Methods: A retrospective study of 52 patients diagnosed with symptomatic FAI was conducted. Evaluation of the spinopelvic complex in terms of SS, LL, SPT, PI and PFA was performed using lateral radiographs of the pelvis and lumbosacral spine in standing and sitting positions. Assessment of hip function at diagnosis was performed using the mHHs. Calculation of spinopelvic mobility was based on the difference (Δ) between measurements performed in standing and sitting position. Results: The median time of pain evolution was 11 months (interquartile range [IQR], 5-24 months) with a median mHHs of 66.0 points (IQR, 46.0-73.0) at diagnosis. The mean change of LL, SS, SPT, and PFA was 20.9±11.2。, 14.2±8.6。, 15.5±9.0。, and 70.7±9.5。, respectively. No statistically significant correlation was observed between spinopelvic parameters and the mHHs (P>0.05). Conclusion: Radiological parameters of the spinopelvic complex did not show correlation with hip function at the time of diagnosis in patients with symptomatic FAI. Conduct of further studies will be required in the effort to understand the effect of the spinopelvic complex and its compensatory mechanics, primarily between the hip and spine, in patients with FAI before and after hip arthroscopy.

IEC61850 기반의 IED 적합성 검사 프로그램 개발

송운식(Un-Sig Song),양효식(Hyo-Sig Yang),김상식(Sang-Sig Kim),박민우(Min-Woo Park),장병태(Byung-Tae Jang),이남호(Nam-Ho Lee),장혁수(Hyuk-Soo Jang) 대한전기학회 2007 대한전기학회 학술대회 논문집 Vol.2007 No.11

IEC6l850 기반의 변전소 자동화 시스템에서 사용하는 IED를 국내의 여러 업체에서 개발하고 있다. 하지만 개발된 IED를 IEC6l850 국제 표준에 맞게 개발했는지 시험하는 적합성 검사는 외국의 인중업체에 의해서만 진행되고 있다. 그렇기 때문에 국내에서도 IED의 적합성 검사를 해줄 수 있는 방법이 있어야 한다. 본 논문에서는 IEC6l850-l0에서 제시하고 있는 ACSI 모델의 매핑과 서비스 시험 사례에 대한 검사를 수행하고 UCA IUG(International Users Group)에서 제시하는 적합성 검사 진행 방법에 따라 검사를 수행할 수 있는 적합성 검사 프로그램의 프로토타입을 구현하고 이를 소개한다.

PICOM에 따른 변전소 자동화 시스템 검사 연구

송운식(Un-Sig Song),양효식(Hyo-Sik Yang),김상식(Sang-Sig Kim),박민우(Min-Woo Park),장병태(Byung-Tae Jang),이남호(Nam-Ho Lee),장혁수(Hyuk-Soo Jang) 대한전기학회 2007 대한전기학회 학술대회 논문집 Vol.2007 No.11

PICOM (Piece of Information for COMmunication)은 논리 노드 간에 교환되는 정보를 기술하기 위하여 정의되어 있다. 변전소의 기능은 여러 논리노드로 분할되어 서로 상호작용하며 동작한다. 변전소 자동화 시스템 검사는 단일 IED만의 기능 시험이 아니며 IED들간에 메시지를 교환을 통하여 변전소 기능을 정의된 성능을 만족하며 수행하는지 검사하는 것이다.

Sequential Conjugation of 6-Aminohexanoic Acids and L-Arginines to Poly(amidoamine) Dendrimer to Modify Hydrophobicity and Flexibility of the Polymeric Gene Carrier

Yu, Gwang-Sig,Yu, Ha-Na,Choe, Yun-Hui,Son, Sang-Jae,Ha, Tai-Hwan,Choi, Joon-Sig Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.2

We synthesized a novel cationic dendrimer consisting of a poly(amidoamine) dendrimer (PAMAM, generation 4) backbone with both L-arginine (Arg) at the termini and 6-aminohexanoic acid (Ahx) between the original core polymer and the peripheral Arg units. The sequential chemical modification of PAMAM G4 with Ahx and Arg resulted in higher transfection efficiency with much less cytotoxicity. PAMAM G4-Ahx-Arg formed stable polyplexes at weight ratios of 8:1 or higher (polymer: plasmid DNA), and the mean polyplex diameter was $180{\pm}20nm$. PAMAM G4-Ahx-Arg showed much higher transfection ability than PAMAM G4 or PAMAM G4-Ahx. Furthermore, PAMAM G4-Ahx-Arg was much less cytotoxic than PEI25KD and PAMAM G4-Arg. In addition to Arg grafting of the PAMAM dendrimer, which endows a higher transfection capability, the addition of Ahx spacer increased dendrimer hydrophobicity, introduced flexibility into the conjugated amino acids, and reduced cytotoxicity. Overall, it appears that the concomitant modification of PAMAM with Ahx and Arg could lead to new PAMAM conjugates with better performances.