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      • KCI등재

        Resistant starch (RS), a novel endogenous inert marker for detecting glucose absorption of small intestine with sweeteners administration in mice

        Wu Yaran,Cai Lei,Xie Xingzi,Yang Shuying,Shi Qing,Jia Hongzhe,Gu Xuqiang,Deng Jingmin,Shi Mingzhao,Chen Qiuping,Cao Shaoqian,Cai Shuangfeng 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.2

        Resistant starch could be degraded by the fermentation of colonic microorganisms in the large intestine of mammals, but not in the small intestine. In this study, we established a novel strategy by using resistant starch as an endogenous marker to determine the glucose absorption of the small intestine of laboratory animals. By optimization of the classical enzymatic method of starch measurement, the demand for the sample weight was reduced by 90%. Moreover, the amount of resistant starch in normal feed was detectable without any extra addition. The value of small intestine glucose absorption of mice was similar when using resistant starch and titanium dioxide as inert markers. The fermentation of resistant starch by intestinal microorganisms in the small intestine was demonstrated not disturbing the detection of glucose absorption significantly. Artificial sweeteners exposed ICR mice showed different glucose absorption which indicated, first, resistant starch can be used as a novel endogenous marker in the small intestine of small animals; second, although glucose tolerance did not change in mice after short-term exposure to artificial sweeteners, there were significant changes in glucose absorption associated with it; third, the short-term exposure resulted in no significant change in glucose tolerance.

      • A Study on the Optimization of Anisotropic Conductive Films for Sn-3Ag-0.5Cu-Based Flex-on-Board Application at a 250 °C Bonding Temperature

        Zhang, Shuye,Yang, Ming,Wu, Yang,Du, Jikun,Lin, Tiesong,He, Peng,Huang, Mingliang,Paik, Kyung-Wook IEEE 2018 IEEE transactions on components, packaging, and ma Vol.8 No.3

        <P>Although cationic epoxy was optimized for low-melting SnBi58 solder ACF joints with the lowest coefficient of thermal expansion (CTE) in terms of reliability, cationic epoxy also showed a faster curing property than any other types of adhesives. In fact, solder joint shapes at 250 °C bonding are very different from those shapes at 200 °C bonding. In this paper, four adhesive film types were investigated in terms of Sn-3Ag-0.5Cu solder ACF joint shapes on the electrical performances and reliability in a pressure cooker test (PCT). Thermal stability of adhesive films was tested to be first. Resin curing speeds were measured in a 250 °C isothermal mode differential scanning calorimetry, resin viscosities were checked by a parallel-plate rheometer, and adhesive thermomechanical properties, such as modulus and CTE, were characterized. Then, four different types of ACF resins containing the same weight percentages of Sn-3Ag-0.5Cu solders were assembled by the same thermocompression bonding parameter (250 °C 10s 2MPa on bump) on a 500- <TEX>$\mu \text{m}$</TEX>-pitch flex-on-board (FOB) application, and different solder joint morphologies were verified. Various bonded solder ACFs joints were compared in terms of solder wetting areas, electrical performances by a four-point-probe method, and the reliability of PCT (121 °C 100% humidity 2atm) for 120 h. This paper aims at optimizing the best adhesive film candidate for SAC305 solder ACF joints of FOB application.</P>

      • KCI등재

        Fault-Tolerant Control of Nonlinear System

        Yingwei Zhang,Shuying Wu,Yuan Wei 제어·로봇·시스템학회 2011 International Journal of Control, Automation, and Vol.9 No.6

        The design strategies of fault-tolerant control system (FTCS) are developed, which are categorized into two types: redundant design and fault compensation. In the first type, a certain degree of redundancy is introduced to controller. The resulting closed-loop system is stable not only when all actuators are operational but also only when one actuator experiences fault. In the second type, the on-line fault-tolerant control (FTC) scheme is developed based on the fuzzy logic system. The closed-loop stability is established based on Lyapunov’s sense. The resulting closed-loop system is stable by using the corrective control law. Compared with the existing results, the fault is accommodated by the fault compensator. The updating control law is so derived that all the parameters of the closed-loop system are bounded. The advantage of redundant design is that it is unnecessary to know the position and magnitude of the fault and is performed without FDI, which reduce the computing time and avoid the false alarms, non-detection and delay due to FDI. The advantage of fault compensation is the dynamics caused by faults that can be accommodated online. Examples of the proposed design indicate that the approaches are effective.

      • Investigation of the super-resolution methods for vision based structural measurement

        Zhi Cong Chen,Lijun Wu,Zhouwei Cai,Chenghao Lin,Shuying Cheng,Peijie Lin 국제구조공학회 2022 Smart Structures and Systems, An International Jou Vol.30 No.3

        The machine-vision based structural displacement measurement methods are widely used due to its flexible deployment and non-contact measurement characteristics. The accuracy of vision measurement is directly related to the image resolution. In the field of computer vision, super-resolution reconstruction is an emerging method to improve image resolution. Particularly, the deep-learning based image super-resolution methods have shown great potential for improving image resolution and thus the machine-vision based measurement. In this article, we firstly review the latest progress of several deep learning based super-resolution models, together with the public benchmark datasets and the performance evaluation index. Secondly, we construct a binocular visual measurement platform to measure the distances of the adjacent corners on a chessboard that is universally used as a target when measuring the structure displacement via machine-vision based approaches. And then, several typical deep learning based super resolution algorithms are employed to improve the visual measurement performance. Experimental results show that super-resolution reconstruction technology can improve the accuracy of distance measurement of adjacent corners. According to the experimental results, one can find that the measurement accuracy improvement of the super resolution algorithms is not consistent with the existing quantitative performance evaluation index. Lastly, the current challenges and future trends of super resolution algorithms for visual measurement applications are pointed out.

      • KCI등재

        Molecular cloning, characterization and expression analysis of bolting-associated genes in flowering Chinese cabbage

        Xufeng Xiao,Caijun Wu,Zhiyun Xu,Yingui Yang,Shuying Fan,Heng Wang 한국유전학회 2015 Genes & Genomics Vol.37 No.4

        Bolting and flowering enhance the commercial value of flowering Chinese cabbage. FLOWERING LOCUS C (FLC) and FRIGIDA (FRI) are two key flowering time genes in Arabidopsis thaliana. Here we reported on the cloning and characterization of three ‘classical’ genes from the autonomous pathway from flowering Chinese cabbage, BrcuFCA, BrcuFLD and BrcuFVE. The results of expression analysis showed BrcuFLC was a gradually up-regulated with the developmental stages. However, temporal mRNA expression of BrcuFRI, BrcuFCA, BrcuFVE, and BrcuFLD were found to follow the opposite transcription patterns. The spatial expression patterns of BrcuFCA, BrcuFLD, and BrcuFVE were similar with the highest levels in flowers, whereas the highest transcription levels of BrcuFLC occurred in leaves and stems and that of BrcuFRI in roots. We presumed that the main pathway of bolting– flowering regulation in flowering Chinese cabbage might be the autonomous pathway and different from the vernalization pathway and FRI-dependent pathway.

      • SCIESCOPUSKCI등재

        Effect of Red Pepper (Capsicum frutescens) Powder or Red Pepper Pigment on the Performance and Egg Yolk Color of Laying Hens

        Li, Huaqiang,Jin, Liji,Wu, Feifei,Thacker, Philip,Li, Xiaoyu,You, Jiansong,Wang, Xiaoyan,Liu, Sizhao,Li, Shuying,Xu, Yongping Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.11

        Two experiments were conducted to study the effects of red pepper (Capsicum frutescens) powder or red pepper pigment on the performance and egg yolk color of laying hens. In Exp. 1, 210, thirty-wk old, Hy-line Brown laying hens were fed one of seven diets containing 0.3, 0.6, 1.2, 2.0, 4.8 or 9.6 ppm red pepper pigment or 0.3 ppm carophyll red. Each diet was fed to three replicate batteries of hens with each battery consisting of a row of five cages of hens with two hens per cage (n = 3). In Exp. 2, 180, thirty-wk old, Hyline Brown laying hens, housed similarly to those in Exp. 1, were fed an unsupplemented basal diet as well as treatments in which the basal diet was supplemented with 0.8% red pepper powder processed in a laboratory blender to an average particle size of $300{\mu}m$, 0.8% red pepper powder processed as a super fine powder with a vibrational mill ($44{\mu}m$) and finally 0.8% red pepper powder processed as a super fine powder with a vibrational mill but mixed with 5% $Na_2CO_3$ either before or after grinding. A diet supplemented with 0.3 ppm carophyll red pigment was also included (n = 3). In both experiments, hens were fed the red pepper powder or pigment for 14 days. After feeding of the powder or pigment was terminated, all hens were fed the basal diet for eight more days to determine if the dietary treatments had any residual effects. In Exp. 1, there were no differences in egg-laying performance, feed consumption or feed conversion ratio due to inclusion of red pepper pigment in the diet. Average egg weight was higher (p<0.05) for birds fed 1.2, 2.4 or 9.6 ppm red pepper pigment than for birds fed the diet containing 0.3 ppm red pepper pigment. On d 14, egg color scores increased linearly as the level of red pepper pigment in the diet increased. In Exp. 2, feeding red pepper powder did not affect egg-laying performance, feed consumption or feed conversion ratio (p>0.05). However, compared with the control group, supplementation with all of the red pepper powder treatments increased egg weight (p<0.05). All the red pepper powder treatments also increased (p<0.05) the yolk color score compared with the control. The results of the present study suggest that both red pepper powder and pigment are effective feed additives for improving egg yolk color for laying hens.

      • KCI등재

        Molecular Identification of Microbial Community in Chinese Douchi During Post-fermentation Process

        Tingtao Chen,Shunqiang Xiong,Shuying Jiang,Mengjuan Wang,Qinglong Wu,Hua Wei 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.6

        To track changes in dynamic microbial communities during post-fermentation process, traditional culture method, and denaturing gradient gel electrophoresis (DGGE) were used to study the number and species of dominant microorganisms in douchi. The result of culturedependent method showed that the microbial number changed slightly since the 4^th day while the DGGE indicated that the really steady-state was achieved from the 10^th day. In addition, Lactococcus lactis subsp.,Staphylococcus lentus, and 2 uncultured bacterium were identified to occupy the dominant positions in bacterial DGGE pattern, and Bacillus thermoamylovorans, Bacillus subtilis, Enterobacter spp., and Absidia corymbifera,Pichia guilliermondii, Pichia farinose were also detected from Bacillus and fungal DGGE patterns, respectively. In conclusion, some pathogenic microorganisms involving in the douchi fermentation had been detected throughout the post-fermentation process, and the combination of culturedependent and –independent method was proved to be effective in profiling microbial diversity.

      • KCI등재

        Oxidative stress-induced circKIF18A downregulation impairs MCM7-mediated anti-senescence in intervertebral disc degeneration

        Wang Jianle,Xia Dongdong,Lin Yan,Xu Wenbin,Wu Yaosen,Chen Jiaoxiang,Chu Junjie,Shen Panyang,Weng Sheji,Wang Xiangyang,Shen Lifeng,Fan Shunwu,Shen Shuying 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

        Low back pain, triggered by intervertebral disc degeneration (IVDD), is one of the most common causes of disability and financial expenditure worldwide. However, except for surgical interventions, effective medical treatment to prevent the progression of IVDD is lacking. This study aimed to investigate the effects of circKIF18A, a novel circRNA, on IVDD progression and to explore its underlying mechanism in IVDD. In this study, we found that oxidative stress was positively correlated with nucleus pulposus cell (NPC) senescence in IVDD and that circKIF18A was downregulated in IVDD and attenuated senescent phenotypes such as cell cycle arrest and extracellular matrix degradation in NPCs. Mechanistically, circKIF18A competitively suppressed ubiquitin-mediated proteasomal degradation of MCM7, and the protective effects of circKIF18A on NPCs were partially mediated by MCM7 under oxidative stress. Intradiscal injection of adenoviral circKIF18A ameliorated IVDD in a rat model. This study revealed that circKIF18A regulates NPC degeneration by stabilizing MCM7 and identified a novel signaling pathway, the circKIF18A-MCM7 axis, for anti-senescence molecular therapy in IVDD.

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