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Shang-Hao Liu,Chen-Feng Kuan,Cing–Yu Ke,Ming-Yuan Shen,Chin-Lung Chiang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.127 No.-
In this study, chitosan (CS) from fish waste was used to prepare bio-based environmentally friendly flameretardants. After the hydroxyl group (OH) of CS and the ammonium group (NH4 + ) of ammoniumpolyphosphate (APP) underwent reactions, they were filtered and dried to obtain CS-APP. The aminogroup of CS-APP then reacted with the epoxy group of 4,40-methylenebis(N,N-diglycidylaniline) (NDY)to form CS-APP-NDY. Isophorone diisocyanate, polyol, and 3-aminopropyltriethoxysilane reacted to formsilicon polyurethane. CS-APP-NDY was then mixed with Si-PU to prepare polymer composites. To determinethe structure, thermal properties, flame retardancy, and mechanical properties of the composites,Fourier-transform infrared spectroscopy, thermogravimetric analysis, limiting oxygen index (LOI), conecalorimetry, UL-94, thermal analysis-FTIR (TA-FTIR) spectroscopy, universal machine testing, scanningelectron microscopy, X-ray photoelectron spectroscopy, and Raman spectroscopy were performed. TheTGA results revealed that, after the addition of CS-APP-NDY, char yield increased from 0.5 wt% to 25.8wt%, and the thermal stability of pristine PU also improved. In addition, the LOI and UL-94 results indicatedthat, after the addition of CS-APP-NDY, the LOI increased from 18.2% to 26.3%, and the UL-94 levelimproved from ‘‘Fail” to V-1. Overall, these results indicated that the addition of CS-APP-NDY to pristinePU increased its flame-retarding performance.
Hao Wang,Yinuo Liu,Yanan Wang,Xiumin Shang,Zhongxin Yan,Shengli Li,Wei Bao 대한부인종양학회 2024 Journal of Gynecologic Oncology Vol.35 No.2
Objective: We previously elucidated that long non-coding RNA Promoter of CDKN1A AntisenseDNA damage Activated RNA (PANDAR) as a p53-dependent oncogene to promote cisplatinresistance in ovarian cancer (OC). Intriguingly, high level of p53-independent PANDAR wasfound in cisplatin-resistant patients with p53 mutation. Here, our study probed the new rolesand the underlying mechanisms of PANDAR in p53-mutant OC cisplatin-resistance. Methods: A2780 and A2780-DDP cells were ser ved as OC cisplatin-sensitive and cisplatin-resistant cells. HO-8910PM cells were subjected to construct chemotherapy-inducedextracellular vesicles (Chemo-EVs). Transmission electron microscopy (TEM) and nanoparticletracking analysis were employed to evaluate Chemo-EVs. Cell viability was assessed using cellcounting kit-8 and colony formation assays. Cell apoptosis was assessed using Annexin V andpropidium iodide staining. The relationships between PANDAR, serine and arginine-rich pre-mRNA splicing factor 9 (SRSF9) were verified by RNA immunoprecipitation and fluorescencein situ hybridization. Tumor xenograft experiment was employed to evaluate the effects ofPANDAR-Chemo-EVs on OC cisplatin-resistance in vivo. Immunofluorescent staining andimmunohistochemistr y were performed in tumor tissue. Results: PANDAR level increased in OC patients with p53-mutation. PANDAR efflux enactedvia exosomes under cisplatin conditions. Additionally, exosomes from OC cell lines carriedPANDAR, which significantly increased cell sur vival and chemoresistance in vitro and tumorprogression and metastasis in vivo. During cisplatin-induced stress, SRSF9 was recruited tonuclear bodies by increased PANDAR and muted apoptosis in response to cisplatin. Besides,SRSF9 significantly increased the ratio of SIRT4/SIRT6 mRNA in OC. Conclusion: Cisplatin-induced exosomes transfer PANDAR and lead to a rapid adaptation ofOC cell sur vival through accumulating SRSF9 following cisplatin stress exposure.
Xiaoxiang Han,Shang-Bin Liu,Wei Yan,Chin-Te Hung,Yanfei He,Pei-Hao Wu,Li-Li Liu,Shing-Jong Huang 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.7
A series of Brønsted-Lewis acidic ionic liquid (BLAIL) catalysts consisting of sulfonated ionic liquid [SO3H-pmim]Cl and Sn(II) chloride have been synthesized and exploited for catalytic transesterification of soybean oil with methanol to biodiesel. The structural and chemical properties of these [SO3H-pmim]Cl-xSnCl2 (x=0-0.8) catalysts were characterized by different analytical and spectroscopic techniques, such as FT-IR, TGA, and NMR. In particular, their acid properties were studied by solid-state 31P NMR using trimethylphosphine oxide as the probe molecule. The BLAIL catalysts were found highly efficient for transesterification reaction due to the introduction of Lewis acidity by SnCl2 in the initially Brønsted acidic [SO3H-pmim]Cl catalyst. The effects of three independent process variables on biodiesel yield were optimized by response surface methodology (RSM). Consequently, an excellent biodiesel yield of 98.6% was achieved under optimized reaction conditions over the BLAIL catalyst with SnCl2 loading (x) of 0.7.
Jun-Li Liu,Jing-Ting Sun,Hui-Lin Hu,Hao-Yuan Wang,Yun-Xi Kang,Tian-Qi Chen,Zhu-Hong Chen,Yu-Xuan Shang,Yu-Ting Li,Bo Hu,Rui Liu 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.1
A growing number of neuroimaging studies have revealed abnormal brain structural and functional alterations in subjects with internet addiction (IA), however, with conflicting conclusions. We plan to conduct a systematic review and meta-analysis on the studies of voxelbased morphometry (VBM) and resting-state functional connectivity (rsFC), to reach a consolidated conclusion and point out the future direction in this field. A comprehensive search of rsFC and VBM studies of IA will be conducted in the PubMed, Cochrane Library, and Web of Science databases to retrieve studies published from the inception dates to August 2021. If the extracted data are feasible, activation likelihood estimation and seed-based d mapping methods will be used to meta-analyze the brain structural and functional changes in IA patients. This study will hopefully reach a consolidated conclusion on the impact of IA on human brain or point out the future direction in this field.
Intracellular Polysaccharide and its Antioxidant Activity by Pleurotus citrinopileatus SM-01
Su-Qian Wu,Shang-Long Gao,Hong-Hong Liu,Xin-Yi Sun,Long Hao,Le Jia,Li-Fei Pang,Shou-Hua Jia,Meng-Shi Jia 한국고분자학회 2013 Macromolecular Research Vol.21 No.6
The extraction parameters of intracellular polysaccharide (IPS) from Pleurotus citrinopileatus SM-01mycelia were optimized, and the in vitro and in vivo antioxidant activities of IPS were investigated. The optimum conditions of IPS extraction were predicted to be an ultrasonic treatment time of 664.09 s, precipitation time of 23.03h and pH 7.36, and IPS yield was estimated at 16.13%. The in vitro inhibition effects of IPS at a dosage of 5 g/L on the superoxide anion, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals were 73.96±4.62%, 69.2±4.37%,and 50.75±4.39%, respectively, which were 72.56±5.08%, 22.83±1.94%, and 43.93±3.26% higher than that of butylated hydroxytoluene (BHT), respectively. The reducing power of IPS was 0.9±0.07, 69.81±5.24% higher than that of BHT. The activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT) and alanine aminotransferase (ALT) in mice blood were 241.38±23.19, 454.95±42.39, 60.32±5.16, and 32.39±2.54 U/mL,respectively, and the malonaldehyde (MDA) level was 9.54±0.72 nmol/mL. The results provided a reference for the large-scale extraction of IPS by P. citrinopileatus SM-01 in industrial fermentation, suggesting that the IPS can be used as a potential antioxidant, which enhances adaptive immune responses.