http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Seung Hyeok Seok (검색결과 357 건)
- 무료
- 기관 내 무료
- 유료
-
-
-
Increasing Production in Korean Shrimp Farms with White-Spot Syndrome Virus PCR-Negative Brood Stock
( Seung Hyeok Seok ),( Min Won Baek ),( Hui Young Lee ),( Dong Jae Kim ),( Myung Sun Chun ),( Jong Sheek Kim ),( Se Ok Chang ),( Jae Hak Park ) 한국미생물생명공학회 2007 Journal of microbiology and biotechnology Vol.17 No.3
-
( Seung Hyeok Seok ),( Min Won Baek ),( Hui Young Lee ),( Dong Jae Kim ),( Yi Rang Na ),( Sung Hoon Park ),( Hyun Kyoung Lee ),( Doug Young Ryu ),( Jae Hak Park ) 한국동물실험대체법학회 2007 한국동물실험대체법학회 학술대회집 Vol.2007 No.1
We first established a transgenic zebrafish line carrying a plasmid (phAhRE-EGFP) for detecting developmental toxicities caused by aryl hydrocarbon receptor (AhR) binding toxicants. The plasmid contains the 5` human cytochrome P4501A1 (CYP1A1) upstream sequence, which AhR ligands target, and enhanced green fluorescent protein (EGFP) gene, an accurate and quick, inducible reporter. To evaluate this system, we treated embryos of wild-type and the transgenic fish with four well-known AhR binding toxicants: 3,3`,4,4`,5-pentachlorobiphenyl (PCB126), 2,3,7,8-tetra-chlorodibenzo-p-dioxin (TCDD), 3-methylcholanthrene (3-MC), and β-naphthoflavone (β-NF). These toxicants induced a dose-dependent increase in morphologic disruption, indicating toxicity at early life-stages. The transgenic zebrafish were sensitive enough to these toxicants to express the CYP1A1 regulated EGFP. Moreover, fluorescence-based quantification of the reporter gene was consistent with dose dependent toxicity of the four model toxicants. In conclusion, the stable transgenic line will allow for extremely rapid and reproducible toxicological profiling of early life-stage embryo development in both aquatic organisms and humans.
-
-
-
In vivo alternative testing with zebrafish in ecotoxicology
Seung-Hyeok Seok,Min-Won Baek,Hui-Young Lee,김동재,나이랑,Kyoung-Jin Noh,Sung-Hoon Park,Hyun-Kyoung Lee,Byoung-Hee Lee,Jae-Hak Park 대한수의학회 2008 Journal of Veterinary Science Vol.9 No.4
Although rodents have previously been used in ecotoxicological studies, they are expensive, time-consuming, and are limited by strict legal restrictions. The present study used a zebrafish (Danio rerio) model and generated data that was useful for extrapolating toxicant effects in this system to that of humans. Here we treated embryos of the naive-type as well as a transiently transfected zebrafish liver cell line carrying a plasmid (phAhREEGFP), for comparing toxicity levels with the well-known aryl hydrocarbon receptor (AhR)-binding toxicants: 3,3’,4,4’,5-pentachlorobiphenyl (PCB126), 2,3,7,8-tetrachlorodibenzo- p-dioxin, and 3-methylcholanthrene. These toxicants induced a concentration-dependent increase in morphological disruption, indicating toxicity at early life-stages. The transient transgenic zebrafish liver cell line was sensitive enough to these toxicants to express the CYP1A1 regulated enhanced green fluorescent protein. The findings of this study demonstrated that the zebrafish in vivo model might allow for extremely rapid and reproducible toxicological profiling of early life-stage embryo development. We have also shown that the transient transgenic zebrafish liver cell line can be used for research on AhR mechanism studies.
-
-
Seok, Seung-Hyeok,Koo, Hye Cheong,Kasuga, Asako,Kim, Yeun,Lee, Eun Gae,Lee, Hyeyoung,Park, Jong-Hwan,Baek, Min-Won,Lee, Hui-Young,Kim, Dong-Jae,Lee, Byeung-Hee,Lee, Yong-Soon,Cho, Sang-Nae,Park, Jae-H Elsevier 2006 Veterinary microbiology Vol.114 No.3
<P><B>Abstract</B></P><P>Skin ulcers, scoliosis, and dropsy-like scale edema were observed in laboratory-maintained zebrafish. Affected fish had multifocal granulomas not only in internal organs such as the liver, intestine, genital organs, kidney, muscle, and spleen but also in the fin, epithelium, gills, and sclera of the eyes. Large numbers of acid-fast-rod-shaped bacteria were observed within the necrotic centers of well-demarcated, multifocal granulomas with Gram's stain and Ziehl–Neelson's stain. The size of the <I>Mycobacterium</I> spp. was 1–2μm×2–3μm with a double-layered cell wall, based upon electron-microscopical features. Definitive diagnosis of these outbreaks was obtained by culture on selective media followed by PCR-restriction fragment length polymorphism analysis (PRA) of the <I>rpoB</I> gene for species identification. The amplified 360-bp products of the <I>rpoB</I> gene of mycobacteria isolated from zebrafish were digested with <I>Msp</I>I restriction enzyme, which revealed unique band patterns matching those of <I>Mycobacterium abscessus</I> and <I>Mycobacterium chelonae</I> which are responsible for skin and soft tissue infection caused by rapidly growing mycobacteria in humans. This is the first documentation of the precise identification of zoonotic non-tuberculous mycobacteria isolated from laboratory-maintained zebrafish by the PRA of the <I>rpoB</I> gene; this study thus provides a great deal of useful epidemiological information and reduces the likelihood that epizootics will occur.</P>
-
Noninvasive imaging of tumor associated macrophages for tumor diagnosis
Seung Hyeok Seok 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7
Metastasis is a series of process in which a primary tumor spreads to secondary organs, and successful colonization of cancer cells at distant sites depends on cooperative interaction with myeloid cells to establish a hospitable environment that is compatible with their growth. Notably, the metastatic niche is established at early stage by tumor-shed factors. However, the molecular mechanism that underlies tumor-induced modulation of myeloid cells to facilitate metastases remains largely uncharacterized. In this talk, I will discuss the recent findings on tumor-induced formation of early metastatic niche, and diagnostic approach of macrophages-targeted noninvasive imaging to detect micrometastases. We identified a tumor-derived factor highly secreted by aggressive breast cancer cells as a major culprit associated with endothelial remodeling to favor macrophage infiltration at metastatic organ. Also, we found that in vivo imaging of macrophages with clinically applicable imaging modalities such as PET and SPECT/CT could detect lung micrometastases in tumor xenograft models. Collectively, our work suggests tumor-derived rewiring of tumor microenvironments that orchestrates the macrophages-mediated metastatic niche, and its future potential for diagnostic and therapeutic interventions.
KISS연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
