아세틸 콜린 유발 검사시 허혈성 심전도 변화와 흉통의 발생이 내피세포 기능장애에 미치는 영향

최철웅(Cheol Ung Choi),나승운(Seung-Woon Rha),김선원(Sun Won Kim),나진오(Jin Oh Na),임홍의(Hong Euy Lim),김진원(Jin Won Kim),김응주(Eung Ju Kim),한성우(Seong Woo Han),박창규(Chang Gyu Park),서홍석(Hong Seog Seo),오동주(Dong Joo Oh) 대한임상노인의학회 2010 대한임상노인의학회지 Vol.11 No.4

연구배경: 아세틸 콜린 유발 검사시 발생하는 흉통과 허혈성 심전도 변화가 아세틸 콜린에 의해 발생하는 관상동맥의 내피세포 기능에 어떤 영향을 주는지 알아보고자 했다. 방법: 흉통으로 내원하여 관상동맥 조영술과 아세틸 콜린 유발검사를 시행받은 1,085명의 환자를 대상으로 하였다. 아세틸 콜린 유발 검사를 하는 동안 허혈성 심전도 변화 및 흉통의 발생 유무에 따른 관상동맥 내피세포 기능의 특징을 비교하였다. 결과: 539명의 환자에서 아세틸 콜린 검사시 내피세포 기능 장애가 발생 하였다. 허혈성 심전도 변화가 있었던 그룹이 심전도 변화가 없었던 그룹보다 흉통이 더 많았고(78.1% vs. 60.8%, P=0.007), 관상동맥 조영술상 다발성 관상동맥 경련이 빈번하였고(59.4% vs. 40.6%, P=0.004), 관상동맥의 경련 정도도 더 심하였다(64.1% vs. 46.5%, P=0.006). 흉통이 있었던 그룹이 흉통이 없었던 그룹보다 허혈성 심전도 변화의 빈도가 더 높았다(14.7% vs. 7.5%, P=0.007). 또한 흉통이 있었던 그룹이 관상동맥 경련이 다발성으로 발생하는 경우가 많았고 (50.7% vs. 29.5%, P<0.001), 미만성으로 발생하는 경우가 많았고(87.1% vs. 75%, P<0.001), 관상동맥의 경련의 정도도 더 심하였다(51.6% vs. 43.5%, P=0.041). 결론: 아세틸 콜린 유발검사시 발생하는 허혈성 심전도 변화와 흉통은 관상동맥 경련의 정도와 경련이 발생하는 관상동맥 개수와 연관이 있다. Background: ECG changes and chest pain during acetylcholine (Ach) provocation tests may constitute important clinical parameters for Ach-induced endothelial dysfunction. We investigated the association between ischemic ECG changes and chest pain during the Ach-provocation test and angiographic characteristics of Ach-induced endothelial dysfunction. Methods: A total of 1,085 patients with anginal symptoms underwent diagnostic coronary angiography (CAG) and Ach-provocation tests. We compared angiographic characteristics of Ach-induced endothelial dysfunction according to the presence of ECG change and chest pain. Results: A total of 539 patients experienced Ach-induced endothelial dysfunction. Patients who experienced ECG change group suffered more frequent chest pain (78.1% vs. 60.8%, P=0.007), angiographically more frequent multiple coronary artery spasm (59.4% vs. 40.6%, P=0.004), and more severe coronary artery spasm (64.1% vs. 46.5%, P=0.006) than patients without ECG change. However, there was no difference in the length of endothelial dysfunction between the groups. The incidence of ischemic ECG changes in patients with chest pain was higher than in patients without chest pain (14.7% vs. 7.5%, P=0.007). Patients who experienced chest pain more often experienced multiple (50.7% vs. 29.5%, P<0.001), diffuse (87.1% vs. 75%, P<0.001), and severe coronary artery spasm (51.6% vs. 43.5%, P=0.041) than patients without chest pain. Conclusion: Ischemic ECG changes and chest pain during the Ach-provocation test are associated with multi-vessel involvement and severe coronary artery spasm. Chest pain is associated with the length of endothelial dysfunction, but ischemic ECG change is not.

Distribution of Cd and Pb Accumulated in Medicinal Plant Roots and Their Cultivation Soils

Seo, Byoung-Hwan,Kim, Hyuck Soo,Bae, Jun-Sik,Kim, Won-Il,Hong, Chang-Ho,Kim, Kwon-Rae 한국토양비료학회 2015 한국토양비료학회지 Vol.48 No.4

In general, plant roots accumulate more heavy metals than the above ground organs such as leaf, stem, and fruit. This implies that root medicinal plants would be an issue with excessive heavy metal accumulation. Therefore, the current study was carried out to investigate the distribution of heavy metal (focused on Cd and Pb) concentrations in soils and medicinal plant roots grown in different region of Korea. Total 293 samples for each soil and plant were collected along the national wide. Soil pH, total and phytoavailable metal concentrations (1 M $NH_4NO_3$ extracted) in soils were determined and heavy metal concentrations in root of the medicinal plants were analyzed. Heavy metal concentrations of the soil samples studied were not exceeded standard limits legislated in 'Soil Environmental Conservation Act', except 2 samples for Cu. However, substantial amount of Cd was accumulated in medicinal plant roots with 29% samples exceeding the standard limit legislated in 'Pharmaceutical Affairs Act' while all plant samples were lower than the standard limit value for Pb. Also the current study demonstrated that cadmium concentrations in the roots were governed by the phytoavailable Cd in soils, which decreased as soil pH increased. From this result, application of heavy metal immobilization technique using a pH change-induced immobilizing agents can be suggested for safer root medicinal plant production.

하천취수가 하천흐름 및 수질에 미치는 영향

서일원(Seo Il Won),송창근(Song Chang Geun) 대한토목학회 2011 대한토목학회논문집 B Vol.31 No.4B

본 연구에서는 상류단 경계조건으로 입력되는 본류 유량에 생성과 소멸로 작용하는 지천유입량과 취수량을 포함하여 취수장에서의 취수가 하천흐름 및 수질에 미치는 영향을 살펴보기 위하여 팔당댐 직하류부터 잠실수중보 구간에 RMA-2 모형과 RMA-4 모형을 적용하였다. 수치모의 결과, 잠실수중보 상류에 위치해 있는 5개 취수장에서의 취수는 해당 하천 구간의 유량을 변화시키게 되며, 이는 하천의 수위, 유속 등 수리학적 인자를 변화시키는 것으로 밝혀졌다. 이러한 취수량 반영에 따른 수위 및 유속 변화는 해당 하천 구간의 수질의 변화를 초래하는 것으로 나타났다. 취수장에서 빠져나가는 유량을 포함하여 모의한 경우, 구의, 자양, 풍납취수장 부근에서 취수에 의한 유량 손실로 인하여 유속구조가 심하게 교란되었으며, 취수를 고려하지 않은 경우에 비해 유속은 평균 25% 낮게, 수위는 1.5 ㎝ 높게 나타났다. 취수를 고려하지 않은 경우 전 구간에 걸쳐 농도분포가 평행하게 나타났으나, 취수의 영향을 고려한 경우 구의, 암사 및 자양 취수장 부근에서의 농도분포가 크게 변화함을 확인할 수 있었다. 또한 취수를 고려한 경우 취수에 의한 유랑소멸로 하류구간에서 취수를 고려하지 않은 경우에 비해 BOD 농도가 높게 나타났다. 따라서 자연하천의 동수역학적 흐름 및 오염물질 혼합거동을 보다 정확히 해석하기 위해서는 지천 합류량 뿐만 아니라 취수장으로부터 유출되어 빠져나가는 취수량을 동시에 고려해야 하는 것으로 판단된다. The water quantity by intake station as well as the tributary flow discharge acting as sink or source were added to the main flow rate in the present study and RMA-2 and RMA-4 models were applied to the reach from Pal-dang dam to Jam-sil submerged weir to investigate the influence of water supply withdrawal on the river flow and water quality. The numerical results revealed that the water supply withdrawal from 5 intake stations located upstream of Jam-sil submerged weir changed the total flow rate and therby induced different hydraulic characteristics in terms of water surface elevation and velocity. The changed flow field by the inclusion of water intake quantity led to the variation of water quality. By the consideration of the water supply withdrawal, the velocity structure was significantly disturbed by the outflowing flow condition nearby Gu-ui, Ja-yang, and Pung-nap intake stations. Furthermore, the mean velocity was lowered by 25% and the stage upstream of Gu-ui station rose upto 1.5 ㎝ compared with the result by exclusion of water intake. In case of no water withdrawal, the distribution of BOD concentration was parallel throughout the domain. However, when the water withdrawal is considered, the distribution of BOD concentration nearby the Gu-ui, Am-sa, and Ja-yang station was signifiantly changed. In addition, the BOD concentration including the intake stations showed higher value at the downstream of the reach due to the loss of the discharge by water withdrawal effect. It is concluded that both the inflow and outflow discharges from tributaries and water intake stations should be included in the numerical simulation to analyze the hydrodynamic behaviors and mixing characteristics more accurately.

The impact of preliminary elements for management innovation adoption on the firm`s profitability

( Uk Jung ),( Chang-hwa Baek ),( Shin-won Seo ),( Sung-uk Lim ) 한국품질경영학회 2015 한국품질경영학회 학술대회 Vol.2015 No.2

Purpose: Many CEOs have often introduced various types of management innovation for the firm``s profitability improvement. If the firm is, however, in a situation in which the firm is not ready to adopt those management innovations, it is not possible to achieve the goal of profit enhancement. In this study, we analyze the relationship between the preliminary elements of management innovation and profitability, and want to introduce the key success factors of management innovation. Methodology/Approach: After investigating preliminary elements of the management innovation adoption through existing literature review, we conducted a questionnaire survey to Korean K-company. Then, by utilizing the structural equation modeling with AMOS, we analyzed the impact of preliminary elements of the management innovation adoption on the profitability. Findings: We found out that there are main preliminary elements of the management innovation adoption directed to profitability enhancement From this finding, it would be possible to assist promoting successful management innovation of the company. Research Limitation/implication: This research has a limitation on that the findings are based on the analysis with subjective measures rather than objective ones for profitability. There is a need for further studies using objective measures such as an actual percentage figure for sales growth or profit change. Originality/Value of paper: By searching and enhancing the preliminary elements of management innovation affecting the profitability, it would be possible to reduce the innovation failure in the future business.

Electro-hyperthermia up-regulates tumour suppressor Septin 4 to induce apoptotic cell death in hepatocellular carcinoma

Jeon, Tae-Won,Yang, Heebum,Lee, Chang Geol,Oh, Sang Taek,Seo, Daekwan,Baik, In Hye,Lee, Eun Hye,Yun, Ina,Park, Kyung Ran,Lee, Yun-Han Informa UK (TaylorFrancis) 2016 International journal of hyperthermia Vol.32 No.6

<P>Purpose: Modulated electro-hyperthermia (mEHT) has been shown to be effective against various types of human tumours, including hepatocellular carcinoma (HCC). Here we aimed to investigate the molecular mechanism underlying the cytotoxic effects of mEHT to HCC cells. Materials and methods: Human liver cancer cell lines, Huh7 and HepG2, were treated with mEHT (42 degrees C/60 min) three times at 2-day intervals. Growth inhibition and apoptotic induction were evaluated using MTS, microscopic analysis, a clonogenic assay, annexin V/PI staining and a ccK18 ELISA. Global changes in gene expression were examined using RNA sequencing to obtain insights into molecular changes in response to mEHT. For in vivo evaluation of mEHT we used HepG2 HCC xenografts grown in nude mice. Results: mEHT suppressed HCC cell proliferation and long-term colony formation through induction of apoptosis. The growth inhibitory effects are induced through a subset of molecular changes. Notably the expression level of septin 4 (SEPT4) (involved in pro-apoptotic activity and growth suppression) was up-regulated, whereas a key regulator of invasiveness G-Protein coupled receptor 64 (GPR64) was repressed. Subsequent Western blotting confirmed that the common increase in tumour suppressor SEPT4 in both Huh7 and HepG2 cells is accompanied by the restoration of cyclin-dependent kinase (CDK) inhibitor p21 and decrease in pro-caspase 7 and pro-caspase 3, thereby accelerating apoptotic signalling in HCC cells. Additionally, mEHT significantly inhibited the growth of human HCC xenografts in nude mice. Conclusions: These findings suggest that apoptotic cell death induced by mEHT is mediated by the up-regulation of tumour suppressor SEPT4 in human HCC cells.</P>

콤바인 HST 전자제어시스템 개발

서신원(Sin-Won Seo),허윤근(Yun-Kun Huh),이제용(Je-Yong Lee),이창규(Chang-Kyu Lee),배근수(Keun-Soo Bae) 충남대학교 농업과학연구소 2011 농업과학연구 Vol.38 No.1

I/An electro-hydraulic transmission having advantages of convenience, safety, simple linking and high power, and an electronic control system were designed and fabricated. In this study, characteristics of the control system were investigated through outdoor tests for evaluation of installation of the system on a combine. Major findings were as followings. 1. Experiment for performance evaluation of the control system was conducted on concrete road. With steering lever in neutral position, driving HST swash plate and left/right wheel speed increased in proportion to driving lever angle. In case of steering control, steering swash plate angle changed in proportion to steering lever angle. This should cause increase in outer wheel speed, but it was observed that HST swash plate was controlled toward neutral to maintain the speed before steering. As a result, speed before steering was maintained despite the change in outer wheel speed by steering HST swash plate angle change. 2. It was observed that the HST system enabled steering with outer wheel maintained at constant speeds while inner wheel speed decreased, which was more stable than conventional mechanical links. In addition, for the selected 5 criteria, experiment showed satisfactory results and it was judged that installation on real vehicle would be feasible. 3. The control system showed response property of appropriate forward/reverse movement and lift/right steering, without causing any problems during experiment on concrete. Result of response property experiment on field operation also showed appropriate control over forward/reverse movement and left/right steering

Distribution of Cd and Pb Accumulated in Medicinal Plant Roots and Their Cultivation Soils

Byoung-Hwan Seo,Hyuck Soo Kim,Jun-Sik Bae,Won-Il Kim,Chang-Ho Hong,Kwon-Rae Kim 한국토양비료학회 2015 한국토양비료학회지 Vol.48 No.4

In general, plant roots accumulate more heavy metals than the above ground organs such as leaf, stem, and fruit. This implies that root medicinal plants would be an issue with excessive heavy metal accumulation. Therefore, the current study was carried out to investigate the distribution of heavy metal (focused on Cd and Pb) concentrations in soils and medicinal plant roots grown in different region of Korea. Total 293 samples for each soil and plant were collected along the national wide. Soil pH, total and phytoavailable metal concentrations (1 M NH₄NO₃ extracted) in soils were determined and heavy metal concentrations in root of the medicinal plants were analyzed. Heavy metal concentrations of the soil samples studied were not exceeded standard limits legislated in ‘Soil Environmental Conservation Act’, except 2 samples for Cu. However, substantial amount of Cd was accumulated in medicinal plant roots with 29% samples exceeding the standard limit legislated in ‘Pharmaceutical Affairs Act’ while all plant samples were lower than the standard limit value for Pb. Also the current study demonstrated that cadmium concentrations in the roots were governed by the phytoavailable Cd in soils, which decreased as soil pH increased. From this result, application of heavy metal immobilization technique using a pH change-induced immobilizing agents can be suggested for safer root medicinal plant production.

Kikuchi-Fujimoto Disease Coexistent with Papillary Thyroid Carcinoma in a Single Lymph Node

Jin Ju Park,Yu Bin Seo,Hyun Chang Choi,Jeong Won Kim,Mi Kyung Shin,Dong Jin Lee,Jacob Lee 순천향대학교 순천향의학연구소 2015 Journal of Soonchunhyang Medical Science Vol.21 No.1

Cervical lymphadenopathy can be developed from various causes such as viral infection, bacterial infection, Kikuchi-Fujimoto disease, tuberculosis, malignancy, and reactive changes. In patients who have malignancy, metastatic lymphadenopathy is possible but it is rare that other concomitant diseases are in the same lymph node. We experienced a case of coexistence of Kikuchi necrotizing lymphadenitis and papillary thyroid carcinoma in a single cervical lymph node. A 38-year-old man who was previously diagnosed with papillary thyroid cancer with cervical lymph nodes metastasis presented with cervical lymphadenopathy. The lymph node biopsy showed tuberculous lymphadenitis. After finishing anti-tuberculosis medication, recurrent lymphadenopathy had developed and a surgical biopsy was performed. At that time, the diagnosis was Kikuchi necrotizing lymphadenitis combined with metastatic papillary carcinoma in a single lymph node.

Poster Session : DNA Damage Response and Genetic Instability ; P53-dependenet chemopreventive mechanism for SeMet in normal cells exposed to methyl methanesulfonate

( Hwa Jin Jung ),( Ju Han Lee ),( Seok Won Jeong ),( Young Ju Lee ),( Sung Vin Yim ),( Seung Joon Park ),( Joo Ho Chung ),( Jee Chang Jung ),( Young R. Seo1 ) 한국생화학분자생물학회 (구 한국생화학회) 2007 생화학분자생물학회 춘계학술발표논문집 Vol.2007 No.-

Safety of a Traditional Korean Medicine, Cheonggan extracts (CGX): A 2-week Single-dose Toxicity Study in SD Rats and Beagle Dogs

Shin, Jang-Woo,Cho, Jung-Hyo,Seo, Dong-Seok,Sung, Nak-Won,Kwon, Min,Son, Chang-Gue The Society of Korean Medicine 2009 대한한의학회지 Vol.30 No.6

Objectives: To evaluate the acute toxic effects and approximate lethal dose of Cheonggan extracts (CGX) in SD rats and beagle dogs. Methods: Male and female rats were divided into 4 groups (Control, CGX 1250, CGX 2500, CGX 5000) respectively and male and female dogs were divided into two groups respectively (Control, CGX 5000) respectively. A single oral dose of CGX was treated to the rats and dogs. Mortality, signs of gross toxicity, and behavioral changes were observed over 14 days. All animals were observed every hour for 4 hours after administration and once a day thereafter for 14 days. Body weights were determined at $0_{th}$, $7_{th}$, and $14_{th}$ days. All surviving animals were sacrificed and necrotized. Major organs were inspected visually for gross findings. Results: No animals died in any of the groups during the experimental period (2 weeks), rats or dogs. Body weights of rats and dogs during the experiment continuously increased in all groups but there was no significant change. No abnormal clinical signs were observed for 2 weeks after a single administration of CGX in any dose group of CGX, rats or dogs. No abnormal findings in major organs were observed in any group of rats or dogs. Conclusion: CGX does not have acute toxic effects in rats or dogs. Therefore, an approximate lethal dose is assumed to exceed 5000 mg/kg in both rats and dogs.