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H9c2 심근 세포주에서 외인성 nitric oxide가 허혈에 의한 세포 독성에 미치는 영향
정성구,장현용,김명천,고영관,정주호,배영미,박원서,김대중,유영민,김성수,임성빈 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4
Background: Nitric oxide(NO) is known to have protective effects on an ischemic heart and to exert triggering effects on ischemic preconditioning. However, the effects of NO during the ischemic period have not been investigated. To investigate the role of exogenous nitric oxide in a model of ischemic heart cell death, we studied the effects of ischemic preconditioning and ischemia in a normal and an ischemic buffer. Methods: Rat cardiac myoblast cells(H9c2) were cultured in a normal and an ischemic buffered medium. For the ischemic culture of heart cells, the cells were cultured in a dessicator with GasPak for 5 hrs. In ischemic preconditioning, the cells were pretreated with ischemic buffer for 5 min and then perfused with normal medium for 30 min. For the measurement of the cytotoxicity, a MTT(3-4-Sdimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay was performed. A DAPI(4',6-diamidino-2-phenylindole dihydrochloride) staining procedure and a flow cytometry analysis were performed to confirm apoptotic cell death by ischemia. Results: Cell viability, as determined by using a MTT assay, showed that the preconditioned group treated with NO showed more cell death than with the not-preconditioned groups in both normal and ischemic buffers. But, In normal medium and not-preconditioned groups, NO showed protective effect according to the concentrations(100,1000μM) . No treatment with NO produced the different results. In normal medium, the protective effect of ischemic preconditioning was demonstrated, but no protective effect of ischemic preconditioning could be seen in the case of the ischemic buffer. The DAPI staining and flow cytometry analysis of heart cells showed characteristic apoptotic features. Conclusion: NO added in the ischemic phase had deterious effects on heart cells. Ischemic preconditioning was more harmful than ischemia alone. The toxicity of the cells was characteristic apoptosis.
Angiotensin 전환효소 유전자 다형성과 양극성 장애
김경나,김종우,정주호,이기철,정홍경,임성빈 대한생물치료정신의학회 2003 생물치료정신의학 Vol.9 No.2
연구목적 : 감정조절에 Renin-Angiotensin System이 관여하는 것으로 보고되어 왔다. 그 근거로 우울증의 새로운 약물치료로 주목받고 있는 Substance P의 대사에 angiotensin 전환효소(angiotensin-converting enzyme, ACE)가 관여한다는 것과 고혈압 환자에서 angiotensin 전환효소 억제제(ACE inhibitor)를 사용했을 때 다행감이나 우울감을 초래한다는 것 등이 있다. 본 연구에서는 ACE의 유전자 다형성을 분석하여 양극성장애와 ACE와의 연관성을 알아보고자 하였다. 방 법 : 양극성장애로 진단된 환자군 82명과 대조군 135명을 대상으로 16번째 intron의 다형성 부위를 가진 시발체 쌍(primer pair)을 사용하며 중합효소 연쇄반응(Polymerase Chain Reaction, PCR)을 시행하여 490bp 산물(I allele)과 190bp 산물(D allele)을 관찰함으로써 ACE 유전자의 유전자형(genotype)의 발현율과 대립유전자(allele)의 빈도를 비교 분석하였다. 결 과 : 양극성장애 환자군과 대조군 사이에서 유전자형의 발현율과 대립유전자적 빈도는 유의한 차이가 없었다. 결 론 : 양극성장애 환자군과 ACE 유전자 다형성 사이에 유의한 관련성은 없었다. 이 결과는 ACE가 양극성 장애의 원인으로 주요한 역할을 한다는 것을 시사하지 못했다. Objectives : A possible participation of the Renin-Angiotensin Systern(RAS) in regulating of the mood has been suggested by reports as follows : the angiotensrn converting enzyme(AGE1 is involved jn the metabolism of the neuropeptide substance P impficated with novel strategies for the pharmacotherapy of depression and the use of ACE inhibitors in hypertensive patients has been associated with euphoric or depressive stales. The aim of the present study was to analyze the role of the ACE gene I/D polymorphism for bipolar disorder. Methods : We examined the frequency of a polymorphism characterized by the insertion or deletion of a 287-bp Alu repeat sequence in intron 16 of the angiotensin converting enzyme gene(located on chromosome 37q233 in groups of patients with bipolar disorder(n=82) compared to healthy control subjects[n=135). ACE genotype was determined by size-analysis of polymerase chain reaction products. Results : The ACE ID polymorphism did not show any difference in allelic frequencies and genotypic distributions between bipolar disorder patients and control subjects. Conclusions : No significant association was found with bipolar disorder and the polymorphism of ACE gene. This finding does not support that ACE I/D polymorphism is a significant risk factor for bipolar disorder.
원보 : 파스틱 정(R)(나테글리니드 90mg)에 대한 글루나테 정(R)의 생물학적 동등성
탁성권 ( Sung Kwon Tak ),이진성 ( Jin Sung Lee ),최상준 ( Sang Joon Choi ),서지형 ( Ji Hyung Seo ),이명재 ( Myung Jae Lee ),강종민 ( Jong Min Kang ),류주희 ( Ju Hee Ryu ),홍승재 ( Seung Jae Hong ),임성빈 ( Sung Vin Yim ),이경태 ( 한국약제학회 2009 Journal of Pharmaceutical Investigation Vol.39 No.2
단보 : 인체 혈장중 라베프라졸의 정량을 위한 LC-MS/MS 분석법 검증 및 단일 용량 투여에 의한 약물동태 연구
탁성권 ( Sung Kwon Tak ),서지형 ( Ji Hyung Seo ),류주희 ( Ju Hee Ryu ),최상준 ( Sang Joon Choi ),이명재 ( Myung Jae Lee ),강종민 ( Jong Min Kang ),이진성 ( Jin Sung Lee ),홍승재 ( Seung Jae Hong ),임성빈 ( Sung Vin Yim ),이경태 ( 한국약제학회 2009 Journal of Pharmaceutical Investigation Vol.39 No.1
Apo-1/Fas (CD95) Gene Polymorphism in Korean Hepatocellular Carcinoma Patients
Kim, Sung-Soo,Hong, Seung-Jae,Ahn, Yun-Gul,Kim, Bong-Seog,Yuh, Young-Jin,Han, Kye-Young,Lee, Hee-Jae,Chung, Joo-Ho,Yim, Sung-Vin,Cho, Jae-Young,Park, Yeon-Hee The Korean Society of Pharmacology 2003 The Korean Journal of Physiology & Pharmacology Vol.7 No.1
It is well known that different expression of Apo-1/Fas (CD95) plays important roles in various tumors and hepatocellular carcinoma (HCC) pathogenesis. Apo-1/Fas mediated apoptosis is one of the important pathways of apoptosis and is known to mediate apoptotic cell death by fas ligand (FasL). To examine the possible relationship between Apo-1/Fas gene polymorphism and HCC susceptibility, MvaI restriction fragment length polymorphism (RFLP) of Apo-1/Fas gene was examined in 94 Korean HCC patients and 240 control subjects. No statistically significant difference in the genotypic distribution and allelic frequencies was found between the control and the HCC. It is, therefore, concluded that Apo-1/Fas gene polymorphism is not associated with HCC susceptibility. Further studies are needed in order to clarify the relationships between genotypes of Apo-1/Fas gene and HCC pathogenesis.
Choi, Han-Sung,Jung, Kyung-Hee,Lee, Seung-Chul,Yim, Sung-Vin,Chung, Joo-Ho,Kim, Youn-Wha,Jeon, Woo-Kyu,Hong, Hoon-Pyo,Ko, Young-Gwan,Kim, Chul-Ho,Jang, Ki-Hyo,Kang, Soon-Ah The Korean Society of Food Science and Nutrition 2009 Journal of medicinal food Vol.12 No.1
This study evaluated whether or not bovine colostrum (BC) is able to treat or prevent intestinal barrier damage, bacterial translocation, and the related systemic inflammatory response syndrome (SIRS) and multiple organ dysfunction syndrome (MODS) in an intestinal ischemia/reperfusion (I/R)-injured rat model. Fifty Sprague-Dawley rats were used. The rats' intestinal I/R injuries were induced by clamping the superior mesenteric artery for 30 minutes. After 3 hours of reperfusion and then twice daily reclamping during the experiment, the experimental group was given BC (4 mL/kg/day) perorally, and the other groups received 0.9% saline and low fat milk (LFM) after intestinal I/R injury. Seventy-two hours later we assessed (1) intestinal damage and intestinal permeability, (2) enteric bacterial count and bacterial translocation, (3) serum albumin, protein, and hepatic enzyme levels, (4) pathologic findings of ileum and lung, (5) activity of oxygen-free radical species, and (6) pro-inflammatory cytokines (tumor necrosis factor-$\alpha$ and interleukin-$1{\beta}$). Intestinal damage, intestinal permeability, and bacterial translocation to other organs were significantly reduced in rats fed with BC after I/R when compared to rats fed LFM/saline after I/R (P <.05). In the evaluation of acute lung injury, neutrophils were found only in the lungs of the saline-fed group after I/R, and the wet/dry ratio of the lung tissue was significantly reduced in the BC-fed group after I/R compared to other I/R groups. A marked difference was found between LFM/saline-fed groups and BC-fed groups regarding malondialdehyde (P < .05) and pro-inflammatory cytokines (P < .01). In conclusion, BC may have beneficial effects in treating and preventing intestinal barrier damage, bacterial translocation and the related SIRS and MODS in the intestinal I/R-injured rat model.
Association between cytochrome P450 promoter polymorphisms and ischemic stroke
KIM, SU KANG,YIM, SUNG-VIN,LEE, BYUNG-CHEOL Spandidos Publications 2012 Experimental and therapeutic medicine Vol.3 No.2
<P>The human cytochrome P450 (CYP) superfamily includes at least 57 genes that encode enzymes with diverse metabolic and biosynthetic functions. This study was conducted in order to investigate the associations between polymorphisms in CYP superfamily genes (CYP11B2, CYP17A1, CYP2B6, CYP2C9, CYP2E1 and CYP7A1) and ischemic stroke (IS). Six single nucleotide polymorphisms (SNPs) of CYP superfamily genes were selected and genotyped by direct sequencing in 121 patients with IS and 321 control subjects. The genetic data were analyzed using SNPStats and SPSS 18.0. Multiple logistic regression models (codominant 1, codominant 2, dominant, recessive and log-additive) were used to evaluate odds ratios (ORs), 95% confidence intervals (CIs) and p-values. The rs179998 SNP of CYP11B2 was significantly associated with IS (p=0.0336 in a log-additive model). The rs3813867 SNP of CYP2E1 was significantly associated with smoking in IS (p=0.0336 in a log-additive model). The rs1799998 SNP of CYP11B2 and rs3808607 of CYP7A1 were related to diabetes mellitus in IS (p<0.05). CYP11B2, CYP2E1 and CYP7A1 SNPs were associated with IS in the population studied. Further study is required to confirm these associations and to determine their biological significance.</P>