Dual Roles of Riboflavin and Its Isoalloxazine Derivatives as a Pro- and Anti-oxidant under Light-stimulated Conditions

Selim Kim,Jungil Hong 한국식품영양과학회 2022 한국식품영양과학회 학술대회발표집 Vol.2022 No.10

Production of Polyhydroxybutyrate (PHB) from Greenhouse Gases (GHGs) Containing CH₄ and CO₂ via Syntrophic Cultivation of Oxygenic Photogranules with Methanotrophs

Selim Saed Selim ASHOOR,Seong-Hoon JUN,Tae Uk KHANG,Jerome HAMELIN,Kim MILFERSTEDT,Jinwon LEE,Jeong-Geol NA 한국생물공학회 2023 한국생물공학회 학술대회 Vol.2023 No.10

Application of Fermented Ulgari-Baechu (Winter Grown Cabbage) Broth using Lactic Acid Bacteria during the Curing Process of Ground Beef

Selim Kim,Seoyoung Lee,Heejeong Kim,Hyeon-Son Choi,Jungil Hong 한국식품영양과학회 2022 한국식품영양과학회 학술대회발표집 Vol.2022 No.10

Evaluation of Photostability and Photosensitizing Properties of Riboflavin and Its Isoalloxazine Derivatives under Different Lights

Selim Kim,Jungil Hong 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

Riboflavin (Rb) and its biotransformed metabolites including flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) have an isoalloxazine ring as a common photoactive moiety. In this study, photostability and photosensitizing properties of Rb, FMN, and FAD were evaluated under different LEDs (Blue, red, green, white) and a regular fluorescent light. Among the derivatives, FAD was most stable under irradiation of all types of light. Among the lights irradiated, Blue LED induced most rapid degradation of the derivatives; their half-lives of Rb, FMN, and FAD at 50 μg/mL under 50 lx Blue LED were 1.28, 0.40, and 4.99 h, respectively. The derivatives were stable under Red LED up to 24 h (2000 lx). Blue LED induced the strongest photosensitizing activities of all Rb derivatives. Enhancement of DCF fluorescence by FMN was most pronounced under light, indicating that ROS generation due to its photosensitizing activity was most prominent. These results indicate that the Rb derivatives were unstable under various lights showing photosensitizing properties, and these characteristics should be considered when exposed to light.

프로바이오틱스 유산균에 의한 질소 산화물 전환 및 철 이온 환원활성

김세림(Selim Kim),홍정일(Jungil Hong) 한국식품과학회 2021 한국식품과학회지 Vol.53 No.6

다양한 유산균들이 생체에 유익한 영향을 주는 프로바이오틱스로 제시되고 있는 가운데, 본 연구에서는 L. kimchicus, L. lactis, L. casei, L. plantarum, LGG 및 Leu. mesenteroides 등 유산균 6종을 선별하여 균주별 nitrate의 환원과 nitrite의 소거, 그리고 철이온에 대한 환원활성을 분석하였다. 사용된 6종의 유산균 중, 5종의 균주에서 72시간 배양시간 동안 nitrate에 대한 환원현상이 나타나지 않았으나, L. kimchicus는 nitrate의 환원을 통해 과량의 nitrite를 생성했다. 한편, nitrite 소거활성은 균주별로 상당한 차이를 보였으며, 특히 L. kimchicus는 nitrate와의 반응과 마찬가지로 가장 높은 소거능을 나타내어 반응시간과 균수에 따른 급격한 배지 중 nitrite 수준 감소를 유도하였다. Ferric ion의 ferrous ion으로의 환원에 있어서도 L. kimchicus가 다른 5종의 균주에 비해 420-1500배의 높은 활성을 보였다. L. kimchicus의 환원활성은 멸균처리 균주나 균체 용해물에서는 거의 발현되지 않아 생균의 대사능력에 의한 것으로 나타났다. 이러한 L. kimchicus의 활성은 프로바이오틱스로서의 효과뿐만 아니라, 관련 환원활성에 의한 NO 등 유용 포스트바이오틱스 대사물에 의한 효과도 기대할 수 있을 것으로 판단된다. Many lactic acid bacteria (LAB) have probiotic properties that exert various health benefits. In this study, the reduction potential of nitrogen oxide compounds and ferric ions by six LAB, including Lactobacillus kimchicus, L. lactis, L. casei, L. plantarum, L. rhamnosus GG, and Leuconostoc mesenteroides were evaluated. The L. kimchicus strain produced a substantial amount of nitrite reduced from nitrate added to the media, whereas the other five LAB strains did not. L. kimchicus also showed the most potent reducing activity of ferric to ferrous ions. However, the reduction potential of the autoclaved L. kimchicus was little pronounced. The scavenging activities of viable LAB or their cell lysates against different radicals were not consistent with the potency of the LAB’s reducing ability. The present results indicate that L. kimchicus has a strong reduction potential for nitrogen oxides in viable status, and that this ability can be used as a probiotic property for various health benefits.

K-pop 가사의 러시아어 번역 양상 및 자연스러움에 대한 코퍼스 기반 연구

김세림 ( Kim Selim ) 연세대학교 유럽사회문화연구소 2023 유럽사회문화 Vol.- No.31

본 논문은 K-pop 가사의 러시아어 번역을 분석 대상으로 삼아 그 양상 및 오류를 고찰한다. 이를 위해 코퍼스 분석을 비롯한 정성적 방법과 정량적 방법을 모두 사용하며, 수작업으로 수정한 한-러 병렬 코퍼스를 기반으로 정렬된 텍스트를 형태-통사적, 의미적 층위에서 비교 분석한다. 본 연구에서는 기존의 노래 가사 번역 이론과 문화소 번역 전략을 고려하여, 프란존(Franzon 2008)이 제안한 '운율적 일치' 와 '의미적 일치'라는 널리 용인되는 개념을 분석틀로 삼는다. 그에 따라 언어 쌍간의 음절 수, 단어 및 문장의 대응관계를 논의한다. 특히 번역의 통사적 자연스러움을 평가하기 위해 한국의 국립국어원에서 구축한 한-러 병렬 코퍼스 2021을 참조 코퍼스로 사용하여 언어별 텍스트 내 단어와 글자 수를 비교한다. 단어, 표현, 그리고 문장의 변조를 대상으로 한 본고의 분석 결과는 한-러 번역의 양상 및 전략과 팬 번역에서의 오류 유형에 대한 이해를 도모할 수 있다. 대중가요에 흔히 등장하는 단어 및 표현이 러시아어에 어떻게 대응하는지 살펴보고 그에 대한 데이터셋을 구축하는 것은 인간 번역 뿐만 아니라 기계 번역의 품질 향상으로도 이어질 수 있다. 또한 본 연구는 양국의 제2언어 학습자에게 도움이 되는 한-러 병렬 코퍼스 혹은 오류 코퍼스를 구축하는 데에도 기여할 것으로 기대된다. This paper examines Russian translations of K-pop lyrics focusing on their patterns and errors. For this purpose, both qualitative and quantitative methods, including corpus analysis are used. Based on the manually revised Korean-Russian parallel corpus, the aligned texts are compared and analyzed at the morpho-syntactic and semantic levels. Considering existing song translation theories and translation strategies of cultural-specific items, this study takes the broadly accepted concepts of ‘prosodic match’ and ‘semantic-reflexive match’ proposed by Franzon(2008) as a framework for data analysis. The number of syllables, word correspondences, and sentence correspondences between the language pairs were discussed accordingly. To evaluate the syntactic naturalness, in particular, the Korean-Russian Parallel Corpus 2021 constructed by the National Institute of the Korean Language was used as a reference corpus to compare the number of words or syllables in the texts. The analysis of words, expressions, and alternations in sentences leads us to a better understanding of the patterns and strategies of Korean-Russian translation and types of translation errors in fan translation. Investigating how words or expressions that commonly appear in popular songs correspond to Russian can segue into constructing datasets, which contribute to the quality of not only human translation but also machine translation. The present study is also expected to contribute to building an accurate Korean-Russian parallel corpus or even an error-annotated corpus which would be helpful to second language learners in both countries.

입자분산용매의 나노임프린팅을 이용한 나노딤플 패터닝 시 크랙 발생 특성

박세림(SeLim Park),김진규(JinGyu Kim),한택우(Teak Woo Han),김욱배(Wook-Bae Kim) 한국트라이볼로지학회 2021 한국트라이볼로지학회 학술대회 Vol.2021 No.4

우육 염지 시 Lactobacillus kimchicus 얼갈이배추 발효물 첨가에 의한 아질산나트튬 저감효과

김세림(Selim Kim),김희정(Hee Jeong Kim),이서영(Seo Young Lee),홍정일(Jungil Hong) 한국식품과학회 2022 한국식품과학회지 Vol.54 No.6

L. kimchicus는 얼갈이배추에 대한 발효과정을 통해 신속하게 상당량의 천연 아질산염을 효과적으로 생산하였으며, 이는 얼갈이배추 중 질산염에 대한 환원효과에 의한 것으로 나타났다. L. kimchicus의 생육은 4℃의 저온조건 및 5% 이상의 염도에서 현저하게 지연되었으나, 25-37℃의 온도, 그리고 2%의 염도는 L. kimchicus의 생육에 큰 영향을 미치지 않았다. 분쇄우육의 염지과정 중 얼갈이배추 발효액 첨가는 OxyMb의 생성을 유의적으로 지연시켰으며, 75℃에서 30분간 가열 처리하여 육색을 고정했을 경우 얼갈이배추 발효액 자체는 염지육의 적색도를 유의적으로 증가시키지 못했다. 그러나 NaNO₂와 조합처리 시 동일량의 NaNO₂ 단독처리 시보다 유의적인 육색의 적색도 증가를 유도하였으며, 48시간 염지 시 얼갈이배추 발효액과 75 ppm NaNO₂를 혼합처리한 경우에도 150 ppm NaNO₂처리군과 유사한 적색도를 나타냈다. 또한 염지육에 잔류하는 아질산염도 얼갈이배추 발효액과의 조합처리 군에서 NaNO₂만을 처리한 군에 비해 유사한 적색도를 보였음에도 유의적으로 낮은 수준을 나타냈다. 본 결과는 L. kimchicus에 의한 얼갈이배추 발효물의 활용이 육류의 염지과정에서 NaNO₂의 저감화 효과를 나타낼 수 있으며, 다양한 채소 발효물을 염지에 활용하여 NaNO₂의 대체제로 사용할 수 있는 가능성을 제시하였다. Sodium nitrite (NaNO₂) is important for developing the color and enhancing the flavor of cured meats, but its safety remains controversial. In this study, the effects of winter-grown cabbage broth fermented by Lactobacillus kimchicus (FB) on ground beef during the curing process were investigated. L. kimchicus produced a substantial amount of nitrite after 24 h of FB fermentation. After 24 h of curing at 4℃, the addition of NaNO₂ or FB delayed oxymyoglobin formation. After heating, the redness of the cured meat was significantly more pronounced with FB plus NaNO₂ added than with the same level of NaNO₂ only added. After 48 h of curing, meat redness was similar between samples cured with FB plus 1/2 the level of NaNO₂ and those cured with NaNO₂ only, whereas the residual nitrite level was significantly lower in samples cured with FB plus 1/2 the level of NaNO₂. The present results suggest that fermented vegetables containing natural nitrite sources may be applied to meat curing process to reduce NaNO₂ usage.

Ultra-fine SnO₂ nanoparticles doubly embedded in amorphous carbon and reduced graphene oxide (rGO) for superior lithium storage

Sher Shah, Md. Selim Arif,Lee, Jooyoung,Park, A. Reum,Choi, Youngjin,Kim, Woo-Jae,Park, Juhyun,Chung, Chan-Hwa,Kim, Jaeyun,Lim, Byungkwon,Yoo, Pil J. Elsevier 2017 ELECTROCHIMICA ACTA Vol.224 No.-

<P><B>Abstract</B></P> <P>SnO<SUB>2</SUB> is a well-studied anode material for lithium ion batteries (LIBs). However, it undergoes severe capacity fading because of a large volume change (∼300%) during cycling. Composites of SnO<SUB>2</SUB> with electro-conductive graphene would deliver improved capacity and rate performance. Nevertheless, achieving the theoretical capacity of SnO<SUB>2</SUB> is still elusive, mainly because of disintegration of the active material from graphene and severe aggregation of SnO<SUB>2</SUB>, or Sn nanoparticles produced upon cycling. To surmount these limitations, in this work, nanocomposites containing ultra-fine sized SnO<SUB>2</SUB> nanoparticles (UFSN) with reduced graphene oxide and amorphous carbon were synthesized in a single step at low temperature and environmentally benign way, in which ascorbic acid was employed as the carbon source and reducing agent. UFSN could decrease the lithium ion diffusion path length. As a result of effective buffering effect afforded by the mesoporous structure against volume change and improved lithium ion diffusivity, the ternary nanocomposite achieves ultra-high capacity of 1245mAhg<SUP>−1</SUP> after 210 cycles at 100mAg<SUP>−1</SUP> and excellent cycling stability. Since the proposed approach is facile, straightforward, and highly reproducible, it is anticipated that this system would be a potential alternative to the conventional graphite anode for LIBs.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

The Korean Society of Gastroenterology & SIDDS 2032 : Slide Session ;K-LG-03 : Lower GI Tract ; Parthenolide Enhances Sensitivity of Colorectal Cancer Cells to Trail by Inducing Death Receptor 5 and Promotes Trail-Induced Apoptosis

( Kyung Bo Yoo ),( Selim Kim ),( Seung Hun Kim ),( In Hee Kim ),( Seung Ok Lee ),( Soo Teik Lee ),( Sang Wook Kim ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising cancer therapeutic agent. Recombinant human TRAIL has been under clinicaltrials, whereas various kinds of malignant tumors have resistance to TRAIL. So, combination therapy with other anticancer agents is a promising strategy to overcome TRAIL resistance. Parthenolide (PT) has recently been demonstrated as a promising anticancer agent and several investigations of combined therapy PT are reported.Methods: In this study, we investigate the molecular mechanisms by which PT sensitizes colorectal cancer (CRC) cells to TRAIL-induced apoptosis. HT-29 (TRAIL-resistant)and HCT116 (TRAIL-senstive) cells were treated with PT and/or TRAIL. The results demonstrated that combined treatment induced apoptosis which was determined using MTT, cell cycle analysis, annexin-V assay, and Hoechst 33258 staining. Results: Interestingly, we confi rm that HCT116 cells have much higher death receptor(DR) 5 than HT-29 cells and PT upregulates DR5 protein level and surface expression in both of cell lines. Apoptosis through the mitochondrial pathway was confi rmed by detecting regulation of Bcl-2 family members, p53 cytochrome C release and caspase cascades. Conclusions: These results suggest that PT sensitizes TRAIL-induced apoptosis via upregulation of DR5 and mitochondrial-dependent pathway. Combination treatment using PT and TRAIL might offer an effective strategy to overcome TRAIL resistance in certain CRC cells.