Isolation and Transcriptional Expression of CuZn Superoxide Dismutase from Codonopsis lanceolata

Lee,Kang,In,Jun-Gyo,Yu,Chang-Yeon,Yun,Song-Joong,Min,Byung-Hoon,Rho,Yeong-Deok,Kim,Moo-Sung,Yang,Deok-Chun 한국자원식물학회 2004 Plant Resources Vol.7 No.3

To investigate the defense mechanism against the abiotic stress, a cDNA clone encoding a CuZn superoxide dismutase (CuZnSOD) protein was isolated from a cDNA library prepared from tabroot mRNAs of Codonopsis lanceolata. The eDNA, designated ClSODCc, is 799 nucleotides long and has an open reading frame of 459 bp with a deduced amino acid sequence of 152 residues. The deduced amino acid sequence of ClSODCc matched to the previously reported CuZnSODs. Consensus amino acid residues (His-45, -47, -62, -70, -79, -119 and Asp-82) were involved in Cu-, Cu/Zn-, and Zn- binding ligands. The deduced amino acid sequence of ClSODCc showed high homologies (82%-86%) regardless of species. Expression of ClSODCc by oxidative stress was increased up to 1 h after treatment and declined gradually. Much earlier and stronger expression of ClSODCc was observed in the cold stress treatment.

Laparoscopic single-port access right adrenalectomy using lee's port : A case report

Sung Hoon Choi,Ho Kyoung Hwang,Chang Moo Kang,Woo Jung Lee 대한외과학회 2010 대한외과학회 학술대회 초록집 Vol.2010 No.11

백서에서 수술 후 복부 유착방지용 재료들의 효능평가

심희수(Hee Soo Shim),이영우(Young Woo Lee1),이영무(Young Moo Lee),오영하(Young Ha Oh),권성원(Sung Won Kwon),김진홍(Jin Hong Kim),손승국(Seung Kook Sohn) 대한외과학회 2002 Annals of Surgical Treatment and Research(ASRT) Vol.63 No.3

Physical and chemical Effects on the sonication Treatment of chitosan solution

Lee, Keun Tai,Park, Seong Min,Park, Chan Kyu,Kim, Sang Moo 한국키틴키토산학회 1997 한국키틴키토산학회지 Vol.2 No.2

물리적 방법을 이용한 키토산 올리고당의 제조에 관한 기초 자료를 제시하기 위하여 키토산용액을 20kHz의 초음파로 처리하였으며, 초음파 처리 효과에 미치는 처리 조건의 영향을 조사하였다. 키토산 용액의 고유점도는 초음파 처리 5분까지는 급속하게 감소하였고 그 이후로는 서서히 감소하였다. 키토산 용액의 부피가 작을수록 초음파 처리효과는 컸으며, 키토산 용액의 온도는 초음파 처리에 별다른 효과가 없는 것으로 나타났다. 5분간 초음파 처리 후의 점도는 용매의 종류에 따라 큰 차이가 없었으나, acetate buffer의 경우 초음파 처리효과가 가장 컸다. pH가 높을수록 초음파 처리효과는 크게 나타났으며, 이온강도와 염의 종류에 따른 효과는 나타나지 않았다. 이상의 결과에서, 용액의 부피는 10~20ml, 온도는 20~30℃, 용액의 pH는 4.5, 그리고 용매의 종류는 acetate buffer로 하는 것이 최적 초음파 처리조건으로 추정된다. As the first step of studies related to production of chitooligosaccharides by physical methods, chitosan solution were sonicated with 20kHz and various treatment effects were examined to present fundamental data of sonicated chitosan solution. Intrinsic viscosity of chitosan solution sharply decreased from 3.76dl/g to 2.90dl/g until 5 minutes of sonication and then slowly decreased. With low volume of chitosan solution, sonication was very effective and temperature of chitosan solution slightly affected the efficiency of sonication. In case of changing the solvent, no significant differences were observed on the effect of sonication, however, acetate buffer had highest sonication effect among various solvents. The sonication effect was increased as the increasement of the value of pH, on the contrary, ionic strength and type of counterions showed no effect on sonication, With these results, we assumed that optimal sonication treatment would be as follows, solution volume was 10 ~20ml, temperature range was 20~30℃ , pH value of solution was 4.5 and type of solvent was acetate buffer

An Analysis Model for the Wave Energy Conversion System with Oscillating Water Column (OWC)

Lee, Sang-Moo 동양대학교 2000 동양대학교 논문집 Vol.6 No.1

부유식 파랑에너지 변환시스템(Oscillating Water Column)에 대한 해석은 입력파와 챔버, 챔버 내 공기의 상호작용으로 인하여 어려움이 많다. 이 논문은 이와 같은 요소를 고려하면서도 쉽고 간편한 해석법을 제시한다. 파랑에너지에 의한 자가발전은 파랑에너지를 기계적 운동으로 변환하고 이를 전기에너지로 변환함으로써 가능하다. 본 논문은 파랑에너지에서 기계적 에너지로 변환하는 과정에 집중하여 그 부분의 성능을 해석한다. 단일 진동수 규칙파가 입력되었을 때에 파에 의하여 챔버의 상하운동이 선형적으로 발생하는 것으로 보며, 이 상하운동에 챔버 내의 압력 영향을 고려하였다. 상하운동과 챔버 내로 투과한 파, 그리고 챔버 내 압력에 의해 발생되는 파에 의해 챔버 내의 상대 운동을 정하고, 그 상대운동에 의한 공기의 압축 팽창과 온도상승을 근사적 열역학적 방정식으로 해석하여 오리피스를 통한 유량을 결정하였다. 얻어진 식은 간단하면서도 관련요소의 영향을 전반적으로 표현한다. 결과에 따르면 고정식의 에너지 변환식은 부유식의 특별한 경우로서 파악되었다. 또한 고정식의 시스템을 그대로 부유식으로 바꿨을 때 그 변환효율은 적어지는 것으로 나타났다. 본 해석법은 계산이 간편하므로 설계 단계에서 유용하게 활용될 수 있을 것으로 기대된다.

THE POSSIBILITY OF MEASUREMENT OF FLUCTUATING CONCENTRATION ON A ROTATING DISK

Lee,Sung Moo 한국화학공학회 1966 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.4 No.1

In the measurement of fluctuating concentration and velocity of turbulence in the concentration polarized system, electrolysis on the fully developed rotating disk electrode, as a cathode, is not able to determine accurately those values, as the width of the disk electrode is too larger compared to the scales of turbulence to provide an uniformly linear accessibility. In order to obtain the uniformly or linearly accessible surface and determine the correlation coefficient of turbulence, the typical RING ELECTRODE is considered. Different thin platinum wires are arranged with different distances in the direction of the streamlines on the disk and the other areas of the surface are artificially insulated. Under the necessary assumptions, herein, a theoretical analysis is consistent with the experimental data obtained by other investigators. The edge effect in the arrangement of the uncoated sections and at the edge of the disk is adjustable with the typical geometry for being compensated with turbulent properties but it must be unavoidably negligible for the theoretical analysis on the rotating disk. Gas evolution, non-uniform current density and overpotentials are also controllable, more or less, with the typical structure of the system. Further investigations of the flow near corner, corrosion, and contamination of the ring electrode surface should be studied.

The Korean Academy of Tuberculosis and Respiratory Diseases : Slide Session ; OS-048 : COPD ; Relationship Between Lung Function and Chest X-Ray Findings in Koreans: A Population-Based Study

( Sang Hoon Lee ),( Joo Han Song ),( Kyung Soo Chung ),( Song Yee Kim ),( Eun Young Kim ),( Ji Jung ),( Young Ae Kang ),( Moo Suk Park ),( Se Kyu Kim ),( Joon Chang ),( Young Sam Kim ) 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1

Background: Chronic obstructive pulmonary disease (COPD) is a major health problem in many countries, and it is estimated to be the third common cause of death by 2020. However, there are few population-based studies to investigate the chest x-ray fi ndings in patients with COPD. Methods: We investigated the relationship of lung function and chest x-ray fi ndings using Korea National Health and Nutrition Examination Survey data. A total of 45,811 subjects were enrolled from July 2008 to December 2012. 13,602 subjects (5,878 male and 7,724 female) who undertook chest x-ray and PFT were analyzed in this study. Chest x-ray fi ndings were interpreted by one radiologist and four pulmonologists, and these readings were re-checked by six other doctors. Chest x-ray fi ndings are divided by 11 categories. PFT results are classifi ed as normal, obstructive, or restrictive pattern. All data analysis was done by SAS 9.2.Results: 10,342 (76.0%) subjects showed normal lung function; 1,776 (13.1%) obstructive pattern; 1,484 (10.9%) restrictive pattern. Inactive tuberculosis was the most common fi nding (7.9%) in this study. In subjects with airway obstruction, inactive tuberculosis (20.1%) was the most common fi nding and inactive other lung disease (6.3%) was the second. Like subject with obstructive lung pattern, inactive tuberculsis (11.3%) and inactive other lungdieseas (5.5%) were most common in subjects with restrictive lung function. Conclusions: To the best of our knowledge, there were no studies to investigate the association of lung function and chest x-ray fi ndings using population based study. In Korea, 20.1% of subjects with airway obstruction showed inactive tuberculosis on chest x-ray. Inactive tuberculosis and inactive other lung disease were most common chest x-ray fi ndings in both subjects with obstructive and restrictive lung function, but the proportion was different between two groups.

Chronic Alcohol Consumption Results in Greater Damage to the Pancreas Than to the Liver in the Rats

Lee, Seong-Su,Hong, Oak-Kee,Ju, Anes,Kim, Myung-Jun,Kim, Bong-Jo,Kim, Sung-Rae,Kim, Won-Ho,Cho, Nam-Han,Kang, Moo-Il,Kang, Sung-Koo,Kim, Dai-Jin,Yoo, Soon-Jib The Korean Society of Pharmacology 2015 The Korean Journal of Physiology & Pharmacology Vol.19 No.4

Alcohol consumption increases the risk of type 2 diabetes. However, its effects on prediabetes or early diabetes have not been studied. We investigated endoplasmic reticulum (ER) stress in the pancreas and liver resulting from chronic alcohol consumption in the prediabetes and early stages of diabetes. We separated Otsuka Long-Evans Tokushima Fatty (OLETF) rats, a type-2 diabetic animal model, into two groups based on diabetic stage: prediabetes and early diabetes were defined as occurrence between the ages of 11 to 16 weeks and 17 to 22 weeks, respectively. The experimental group received an ethanol-containing liquid diet for 6 weeks. An intraperitoneal glucose tolerance test was conducted after 16 and 22 weeks for the prediabetic and early diabetes groups, respectively. There were no significant differences in body weight between the control and ethanol groups. Fasting and 120-min glucose levels were lower and higher, respectively, in the ethanol group than in the control group. In prediabetes rats, alcohol induced significant expression of ER stress markers in the pancreas; however, alcohol did not affect the liver. In early diabetes rats, alcohol significantly increased most ER stress-marker levels in both the pancreas and liver. These results indicate that chronic alcohol consumption increased the risk of diabetes in prediabetic and early diabetic OLETF rats; the pancreas was more susceptible to damage than was the liver in the early diabetic stages, and the adaptive and proapoptotic pathway of ER stress may play key roles in the development and progression of diabetes affected by chronic alcohol ingestion.

AEG-1 is Associated with Invasion and Poor Prognosis in Colorectal Cancer

Moo-jun Baek,Tae Sung Ahn,Myung Won Son,Hae Il Jeong,Jong Eun Lee,Chang Hwan Lee,Saeng Jin Park,Jong Hyuk Yun,Ji Hyeon Ahn 대한종양외과학회 2015 대한임상종양학회 학술대회지 Vol.2015 No.2

Effects of Stocking Density or Group Size on Intake, Growth, and Meat Quality of Hanwoo Steers (Bos taurus coreanae)

Lee, Sang-Moo,Kim, Jae-Yeon,Kim, Eun-Joong Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.11

This study was conducted to investigate the effects of stocking density or group size on feed intake, daily gain, and carcass characteristics of Hanwoo (Korean indigenous breed) steers reared from 7 months to 31 months of age. Thirty Hanwoo steers were divided into four groups with three replicates each (a total of 12 pens). In each group, one (G1), two (G2), three (G3), and four steers (G4) per pen were allocated as treatments. Pen size was $32.0m^2$, and therefore Hanwoo steers in G1, G2, G3, and G4 were reared under different space allowances, i.e. 32.0, 16.0, 10.6, and $8.0m^2$/steer, respectively. Steers were reared following a conventional beef cattle management method in Korea, and were offered a fixed amount of commercial concentrate with ad libitum forages. Results were subjected to analysis of variance with stocking density as the main effect, and significance was declared at p<0.05. Although total feed intake was not significantly altered, it numerically increased in animals of low stocking density (G1) compared to those subjected to high stocking density treatment (i.e. G4). Feed conversion ratio was higher (p<0.05) in G3 compared to G1 and G2. Animals in G1 (low stocking density) grew faster (p<0.05) than those of high stocking density (G3 and G4). Back fat thickness, meat yield index, and meat yield grade were similar among all levels of stocking density. However, longissimus muscle area was larger in G1 and G2 (p<0.01) compared to G3 and G4, and animals in G3 produced smaller carcasses (p<0.05). Carcass quality traits, including marbling score, meat color, fat color, texture, maturity and meat quality grade, as determined by a group of experts, were not significantly different among the treatments. In conclusion, lower stocking density resulted in increased feed efficiency, daily gain, and carcass weight in Hanwoo steers. However it remains unclear whether such differences are the results of stocking density or group size, or a combination of both. Nonetheless, these results confirm previous studies reporting a negative effect of increased stocking density on animal productivity. Further, animal welfare under an intensive farming system in relation to economical return is discussed.