유우개량을 위한 유단백질의 유전적 다형에 관한 연구 Ⅰ. αS1 - Casein 및 K - Casein 의 유전적 변이체

한상기(Sang K . Han),이기만(Khy M . Lee),정의용(Eui Y . Chung),장경진(Kyung J . Jang) 한국축산학회 1984 한국축산학회지 Vol.26 No.3

Genetic variants of αs₁-casein (αs₁-Cn) and K-casein (k-Cn) in milk proteins from 138 individual Holstein cattle in Korea were investigated by means of strach-gel-urea electrophoresis and the appearance of phenotypes, gene frequencies and genetic similarities were compared with those of foreign Holstein and of her breeds. The results are summarized as follows: 1. The milk proteins in cattle were controlled by codominant autosomal allelic genes of 9αs₁-Cn^A and αs₁-Cn^B(2 types), and K-Cn^A and K-Cn^B (2 types). 2. The distribution of phenotypes in each milk protein loci was αs₁-Cn BB 124, BC 14; K-Cn AA 76 Ab 49, BB 13. The number of phenotypes coxesponded closely to expectation of Hardy-Weinberg equilibrium (αs₁-Cn:0.7 $gt;P$gt;0.5, k-Cn;0.7$gt;P$gt;0.5). 3. Gene frequencies for genetic variants of the milk protein loci were analyzed. By the predominant allele in αs₁-Cn was αs₁-Cn B with a frequency of 0.949, whereas αs₁-CnC was in low gene frequency (0.051). Among the two alleles for K-Cn, K-Cn A gene showed to occur in high frequency (0.728). However, the Kin B was in low gene frequency (0.272).

Ir 전극 계면에서 위상이동 방법 및 상관계수를 이용한 수소의 흡착동온식 결정

전상규,Jeon, Sang-K. 한국전기화학회 2007 한국전기화학회지 Vol.10 No.2

백금족/수용액 계면에서 Langmuir, Frumkin, Temkin 흡착등온식(${\theta}\;vs.\;E$)을 결정하기 위해 최적중간주파수 일 때 위상이동($0^{\circ}{\leq}-{\varphi}{\leq}90^{\circ}$) 거동($-{\varphi}\;vs.\;E$)과 표면피복율($1{\geq}{\theta}{\geq}0$) 거동(${\theta}\;vs.\;E$) 사이의 선형 관계식 연구에 관한 위상이동 방법 및 상관계수를 제안하고 증명하였다. Ir/0.1 M KOH수용액 계면에서 음극 $H_2$ 발생 반응에 관한 수소의 Langmuir 및 Temkin 흡착등온식(${\theta}\;vs.\;E$), 평형상수(Langmuir 흡착등온식: $K=3.3{\times}10^{-4}mol^{-1}$, Temkin 흡착등온식: $K=3.3{\times}10^{-3}{\exp}(-4.6{\theta})\;mol^{-1}$), 상호작용 파라미터(Temkin 흡착등온식: g=4.6), 표준자유에너지($K=3.3{\times}10^{-4}mol^{-1}$ 일 때 ${\Delta}G_{ads}^0=19.9kJ\;mol^{-1},\;K=3.3{\times}10^{-3}{\exp}(-4.6{\theta})\;mol^{-1}$ 및 $0.2<{\theta}<0.8$일 때 $16.5<{\Delta}G_{\theta}^0<23.3kJ\;mol^{-1}$)를 결정한다. 수소 흡착부위의 비균일 및 측 방향 상호작용 효과는 무시할 수 있다. ${\theta}$의 중간값 즉, $0.2<{\theta}<0.8$일 때 Langmuir 또는 Frumkin 흡착등온식과 상관관계에 있는 Temkin 흡착등온식은 상관계수를 이용하여 쉽게 결정할 수 있다. 위상이동 방법 및 상관계수는 흡착동온식(${\theta}\;vs.\;E$) 및 연관된 전극속도론과 열역학 파라미터(K, g, ${\Delta}G_{ads}^0, {\Delta}G_{\theta}^0$)를 결정하기 위한 정확하고 확실한 기술 및 방법이다. The phase-shift method and correlation constants for studying a linear relationship between the behavior ($-{\varphi}\;vs.\;E$) of the phase shift ($0^{\circ}{\leq}-{\varphi}{\leq}90^{\circ}$) for the optimum intermediate frequency and that (${\theta}\;vs.\;E$) of the fractional surface coverage ($1{\geq}\theta{\geq}0$) have been proposed and verified to determine the Langmuir, Frumkin, and Temkin adsorption isotherms (${\theta}\;vs.\;E$) at noble metal/aqueous electrolyte interfaces. At an Ir/0.1 M KOH aqueous electrolyte interface, the Langmuir and Temkin adsorption isotherms (${\theta}\;vs.\;E$), equilibrium constants ($K=3.3{\times}10^{-4}\;mol^{-1}$ for the Langmuir and $K=3.3{\times}10^{-3}{\exp}(-4.6{\theta})\;mol^{-1}$ for the Temkin adsorption isotherm), interaction parameter (g = 4.6 for the Temkin adsorption isotherm), and standard free energies (${\Delta}G_{ads}^0=19.9kJ\;mol^{-1}\;for\;K=3.3{\times}10^{-4}\;mol^{-1}$ and $16.5<{\Delta}G_{\theta}^0<23.3\;kJ\;mol^{-1}\;for\;K=3.3{\times}10^{-3}{\exp}(-4.6{\theta})\;mol^{-1}\;and\;0.2<\theta<0.8$) of H for the cathodic $H_2$ evolution reaction are determined using the phase-shift method and correlation constants. The inhomogeneous and lateral interaction effects on the adsorption of H are negligible. At the intermediate values of ${\theta},\;i.e,\;0.2<{\theta}<0.8$, the Temkin adsorption isotherm (${\theta}\;vs.\;E$) correlating with the Langmuir or the Frumkin adsorption isotherm (${\theta}\;vs.\;E$), and vice versa, is readily determined using the correlation constants. The phase-shift method and correlation constants are accurate and reliable techniques to determine the adsorption isotherms (${\theta}\;vs.\;E$) and related electrode kinetic and thermodynamic parameters(K, g, ${\Delta}G_{ads}^0, {\Delta}G_{\theta}^0$).

The Phase-Shift Method for the Langmuir Adsorption Isotherms of Electroadsorbed Hydrogens for the Cathodic H₂ Evolution Reactions at the Poly-Pt Electrode Interfaces

천장호,전상규,이재항,Chun, Jang H.,Jeon, Sang K.,Lee, Jae H. The Korean Electrochemical Society 2002 한국전기화학회지 Vol.5 No.3

순환전압전류 및 교류임피던스 기법을 이용하여 다결정 Pt/0.5M $H_2SO_4$ 및 0.5M LiOH수용액 계면에서 저전위 수소흡착(UPD H) 과 전위 수소흡착(OPD H)에 관한 Langmuir 흡착등온식 $({\theta}\;vs.\;E)$ 을 연구조사 하였다. 계면에서 치적중간주파수일 때 위상이동$(0^{\circ}{\leq}{-\phi}{\leq}90^{\circ})$ 거동은 표면피복율$(1{\geq}{\theta}{\geq}0)$ 거동에 정확하게 상응한다. 위상이 동 방법 즉 최적중간주파수일 때 위상이동 변화$({-\phi}\;vs.\;E)$는 계면에서 음극 $H_2$ 발생 반응에 관한 UPD H와 OPDH의 Langmuir흡착등온식을 결정할 수 있는 새로운 전기화학적 방법으로 사용할 수 있다 다결정 Pt/0.5M $H_2SO_4$ 수용액 계면에서 OPD H의 흡착평형상수(K)와 표준자유에너지$({\Delta}G_{ads})$는 각각 $2.1\times10^{-4}$와 21.0kJ/mol 이다. 다결정 Pt/0.5M LiOH 수용액 계면에서 K는 음전위(E)에 따라 2.7 (UPD H)에서 $6.2\times10^{-6}$ (OPD H) 또는 $6.2\times10^{-6}$(OPD H)에서 2.7 (UPD H)로 전이한다. 유사하게 ${\Delta}G_{ads}$는 E에 따라 -2.5kJ/mol (UPD H)에서 29.7kJ/mol (OPD H)또는 29.7kJ/mol (OPD H)에서 -2.5kJ/mol (UPD H)로 전이한다. K와 ${\Delta}G_{ads}$의 전이는 다결정 Pt전극 표면의 상이한 UPD H와 OPD H의 흡착부위에 기인한다. 다결정 Pt전극 계면에서 UPD H와 OPD H는 음극 $H_2$ 발생 반응에 따른 순차적 과정이 아니라, 수소 흡착부위 자체에 따른 독립적 과정이다. UPD H와 OPD H의 기준은 음극 $H_2$발생 반응과 전위가 아니라, 수소 흡착부위와 과정이다. 수용액에서 음극 $H_2$발생 반응에는 다결정 Pt선 전극이 단결정 Pt(100)원반 전극보다 더 효율적이고 유용하다 위상이동 방법은 열역학적 방법과 상충적이 아니라, 보완적이다. The Langmuir adsorption isotherms of the under-potentially deposited hydrogen (UPD H) and the over-potentially deposited hydrogen (OPD H) at the poly-Pt/0.5M $H_2SO_4$ and 0.5 M LiOH aqueous electrolyte interfaces have been studied using cyclic voltammetric and ac impedance techniques. The behavior of the phase shift $(0^{\circ}{\leq}{-\phi}{\leq}90^{\circ})$ for the optimum intermediate frequency corresponds well to that of the fractional surface coverage $(1{\geq}{\theta}{\geq}0)$ at the interfaces. The phase-shift method, i.e., the phase-shift profile $({-\phi}\;vs.\;E)$ for the optimum intermediate frequency, can be used as a new electrochemical method to determine the Langmuir adsorption isotherms $({\theta}\;vs.\;E)$ of the UPD H and the OPD H for the cathodic $H_2$ evolution reactions at the interfaces. At the poly-Pt/0.5M $H_2SO_4$ aqueous electrolyte interface, the equilibrium constant (K) and the standard free energy $({\Delta}G_{ads})$ of the OPD H are $2.1\times10^{-4}$ and 21.0kJ/mol, respectively. At the poly-Pt/0.5M LiOH aqueous electrolyte interface, K transits from 2.7(UPD H) to $6.2\times10^{-6}$ (OPD H) depending on the cathode potential (E) and vice versa. Similarly, ${\Delta}G_{ads}$ transits from -2.5kJ/mol (UPD H) to 29.7kJ/mol (OPD H) depending on I and vice versa. The transition of K and ${\Delta}G_{ads}$ is attributed to the two distinct adsorption sites of the UPD H and the OPD H on the poly-Pt surface. The UPD H and the OPD H on the poly-Pt surface are the independent processes depending on the H adsorption sites themselves rather than the sequential processes for the cathodic $H_2$ evolution reactions. The criterion of the UPD H and the OPD H is the H adsorption sites and processes rather than the $H_2$ evolution reactions and potentials. The poly-Pt wire electrode is more efficient and useful than the Pt(100) disc electrode for the cathodic $H_2$ evolution reactions in the aqueous electrolytes. The phase-shift method is well complementary to the thermodynamic method rather than conflicting.

우리나라 제품의 수출경쟁력 추이에 관한 연구

성극제 경희대학교 사회과학대학 1997 社會 科學 論叢 Vol.15 No.-

알콕시기와 아릴옥시기가 치환된 폴리포스파젠의 합성 및 특성에 관한 연구

성용길,백형기,손연수 동국대학교 산업기술대학원 1993 산업기술논문집 Vol.1 No.-

Polyphosphazenes are represented as a general formula [(R_1R_2)p=N]_n, where R_1 and R_2 are representing organic substituent groups. Poly[(2,2,2-trifluoroethoxy)(p-chlorophenoxy)phosphazenes] were synthesixed by the 2,2,2-trifluoroethoxy and p-chlorophenoxy. The polymers were characterized by means of element analysis, 1^H-NMR,(31)^P-NMR, FT-IR and gel permeation chromatograpy. The weights of all the polymers are above 10^6, where their chromatogrames, in particular, exhibit a monomodal and narrow molecular weoght distribution with low (M￣w/Mn) (2.7∼3.77) values in contrast to the bimodal and broad (M￣w/Mn〉10) molecular weight distributions in known poly(organophosphazenes) systems. The thermal properties of the copolymers reveal appropriately median values between those of each homopolymers. The glass temperatures of the copolymers exhibit between -47℃ and 16℃. The thermal degradation starts at 334℃ and ends up to 460℃ with two step decompositions. The tensile strength of the copolymers is intermediate between those of each homopolymer. However, ultimate elongation values show higher valve than those of each homopolymers.

금속파편충격에 의한 강판의 가속도신호 특성

성게용,윤용구 한국비파괴검사학회 1992 한국비파괴검사학회지 Vol.12 No.2

Acceleration signal characteristics of a steel plate, impacted by steel balls, were studied in an attempt to apply the experimental results to the impact location and mass estimation of metallic loose parts in the cooling system of nuclear power plants. Experimental results show that the variation of maximum acceleration amplitude and impact contact time due to the change of ball mass and impact velocity can be well explained by the Hertz impact theory. The frequency spectral pattern shifted slightly in spite of the increase of impact velocity and impact location. Ball mass, however, strongly affected the frequency spectral pattern. Hence the frequency spectrum can be used for estimation of the mass of unknown loose parts in the cooling system.

K-NOW: Korea Network for Observation and prediction of ice sheet and sea level changes in a Warming world

Won Sang Lee,Emilia Kyung Jin,Choon-Ki Lee,Sukyoung Yun,Ji Sung Na,Hyun-Ju Lee,Byeong-Hoon Kim,Clare Eayrs,Jinseok Kim,Jiyeon Lee,Jinhoon Jung,In-Woo Park,Donghyuck Lee,K-NOW team members 한국기상학회 2023 한국기상학회 학술대회 논문집 Vol.2023 No.10

비육후기 한우수소에 있어서 한약재박 펠렛사료의 대체급여효과

성경일,정종원,김상록 한국동물자원과학회 2001 한국축산학회지 Vol.43 No.6

본 연구는 한약재박 펠렛(POMR)을 비육후기의 한우수소에 일부 대체 급여하였을 때 증체량, 사료섭취량, 사료비 및 도체등급에 미치는 영향에 대하여 검토하였다. 대조구의 배합사료 섭취량은 9.8㎏, POMR구의 배합사료 및 POMR의 섭취량은 각각 8.5 및 2.7 ㎏ 이었으며, 총 건물섭취량은 대조구 13.2 ㎏, POMR구 16.3 ㎏으로 POMR구에서 많았다. (p<0.05). 일당 증체량은 대조구 0.89 ㎏, POMR구 0.83 ㎏으로 대조구에서 다소 높은 경향을 보였으나 처리간에 유의적인 차이는 없었다. 조단백질 섭취량은 대조구 1.80 ㎏, POMR구 2.11 ㎏, TDN 섭취량은 대조구 8.2 ㎏, POMR구 9.9 ㎏ 으로 모두 POMR구에서 높았으며, 조단백질 및 TDN 섭취량은 두 처리구 모두 영양소요구량을 충족하였다. 실험기간동안 두당 총사료비는 대조구 234,402원, POMR구 223,894원으로 POMR구가 13,508원(5.7%) 절감되었다. 도체중은 POMR구가 높은 경향을 보였으며, 등지방두께는 대조구에서 높았다(p<0.05). 배최장근단면적 및 육량지수는 처리구간에 차이가 없었다. 근내지방도 등급은 POMR구가 대조구보다 양호한 경향을 보였으며, 육색 및 지방색은 처리구간에 차이는 없었다. 조직감은 POMR구가 대조구보다 양호하였다(p<0.05). 이상에서 비육후기의 한우수소에 POMR로 일부 대체 급여하여도 조단백질 및 TDN 섭취량은 영양소요구량을 충분히 충족하였으며, 증체량에 차이가 없어 사료급여량의 감소로 사료비가 절감되었다. 육질등급에서 POMR구가 대조구보다 양호하게 나타나 앞으로 육질과 한약재박 펠렛사료의 미량성분과의 관계를 검토할 필요가 있을 것으로 사료된다. To evaluate the effect of partial substitution of a diet by pellets of oriental herbal medicine residues (POMR) on body weight gain, feed intake, feed cost and carcass characteristics, twenty Korean native bulls (485 ㎏, initial BW) in the fattening period were randomly assigned to a control diet or POMR diet. Dry matter (DM) intake for concentrate was higher in the control diet than that of the POMR diet, but DM intake of concentrate plus POMR for the POMR diet was higher than that of the control diet. Total DM intake was higher (P<0.05) in the POMR diet than that of the control diet. Average daily gain appeared to be higher in the control diet than that of the POMR diet, but there was no significant different. Crude protein and TDN intake were higher in the POMR diet than that of the control diet. Estimated crude protein and TDN intakes in both diets exceeded the requirements of the Japanese Feeding Standard for Beef Cattle. Feed cost per bull was reduced by 5.7% for the POMR diet compared to the control diet. Backfat thickness was higher (P<0.05) in the control diet than that of the POMR diet, but rib-eye area and carcass meat production index did not differ between two diets. Marbling score tended to be higher in the POMR diet than that of the control diet, but there was no difference in meat color and fat color. These results indicate that the pritial substitution of POMR may increase economic return without impairing animal performance in Korean native bulls for fattening period. Further study is necessary to evaluate the relationship between trace mineral composition of POMR and beef quality.

한국 토종닭고기의 숙성중 부위별 근원섬유 단백질의 변화

성삼경,안동현,권연주,박소연 한국축산학회 1997 한국축산학회지 Vol.39 No.5

Postmortem changes were examined in myofibrillar proteins of M. pectoralis, M. sartorius and M. soleus from Korean native chickens(KNC) and commercial broilers. Purified myofibrils were prepared from the muscles after 0, 3, 6, 12, 24, 48, 72 and 120 hours of post mortem storage at 4℃. SDS-PAGE results showed that 30kDa and 32kDa components appeared earlier in the M. pectoralis than in the M. sartorius and M. coleus from KNC and broilers. These components were observed after 12 hours post mortem in all M. pectoralis. Titin I disappeared after 12 hours post mortem in M. pectoralis from KNC, earlier than that of broilers. In M. sartorius, thin II appeared after 6 hours post mortem in KNC, earlier than that of broilers, and thin I disappeared after 120 hours of storage. Titin II appeared after 24 hours post mortem in M. Soleus from KNC and broilers. Among all the samples studied, the post mortem changes in M. Pectoralis was the fastest and those changes in M. coleus was the slowest. The molecular weight of the titin in M. coleus and M. sartorius is larger than that of M. pectoralis from KNC.

재래산양 락토페린 유전자 5' Flanking 영역의 특성에 관한 연구

상병찬,유대열,유성란,이태훈,신상태,이경광 한국축산학회 2000 한국축산학회지 Vol.42 No.1

Lactoferrin(Lf), a ferric ion(Fe^(3+))-binding glycoprotein, is found most notably in milk and some exocrine secretions. It is a main antimicrobial component of colostrum and milk that contributes to protection of the infant against infectious diseases. To analyze 5' flanking region of Korean native goat lactoferrin gene, we cloned and sequenced 975 by of 5' flanking region of Korean native goat Lf gene. When compared to those of bovine and human Lf, the nucleotide sequences exhibited about 90.9% and 49.7% homology, respectively. The transcription start site was localized by primer extension to residue C, which is 38 nucleotides upstream from the ATG start codon. The 5' flanking region of Korean native goat lactoferrin gene contains putative transcription factor binding sites such as SP1, CAT, C/EBP and GATA-1.