광산 폐석의 알칼리 반응성에 대한 광물.암석학적 연구

임한욱,최성범,백환조,김덕현 강원대학교 석재복합신소재제품연구센터 1997 석재연 논문집 Vol.2 No.-

석회암을 이용한 골재의 알칼리 반응성을 측정하기 위하여 한국공업규격의 표준시험 방법에 의한 팽창성을 측정하였다. 기존의 연구결과에 의하면 특히 백운석을 포함하는 석회암의 경우 골재-알칼리 반응이 상당한 것으로 알려지고 있으나, 본 연구에 사용된 석회암 시료의 경우는 알칼리 반응성이 매우 작은 것으로 나타났다. 또한, 역학적 특성의 결과도 매우 양호하며 골재로서의 활용성이 충분한 것으로 판단된다. An experimental program was undertaken to investigate the alkali-aggregate reaction of limestone aggregates, following the standard testing method for potential alkali reactivity of carbonate rocks for concrete aggregates. It is generally known tat dolomitic limestones may exhibit high potential of alkali-aggregate reaction. However, the limestone samples employed in this study showed negligible effect of the alkali reaction, even with substantial dolomite content. It is also concluded that the mechanical properties of the limestone aggregates are adaptable for concrete aggregates

정형외과 영역에 있어서 Ketorolac Tromethamine과 복합제(Aspirin/Acetaminophen/Caffeine Anhydrous)와의 비교 임상적 연구

이광진,임상덕,김석범 최신의학사 1989 最新醫學 Vol.32 No.2

The comparison of gene expression of inflammatory mediators between palmoplantar pustulosis and pompholyx

( Do Young Kim ),( Ji Young Kim ),( Hyojung Sohn ),( Taegyun Kim1 ),( Jihun Park ),( Beom Jin Lim2 ),( Sang Ho Oh ) 대한피부과학회 2012 대한피부과학회 학술발표대회집 Vol.64 No.3

Proteome Analysis of Chicken Embryonic Gonads: Identification of Major Proteins from Cultured Gonadal Primordial Germ Cells

Sang In Lee,Beom Ku Han,Sang Hyun Park,Tae Min Kim,Sang Soo Sin,Young Mok Lee,Hee Bal Kim,Jeong Mook Lim,Jae Yong Han 한국가금학회 2005 한국가금학회 정기총회 및 학술발표회 Vol.22 No.-

배자 생식세포 발달에 관련된 메카니즘을 밝혀내기 위해서, 닭 배자 생식기에서 추출한 원시 생식세포의 단백질체 지도를 만들었다. 총 500 배자를 6일간 배양하여 배자 생식기를 획득했고, 7-10일 배양 후, 배양된 원시 생식세포는 2차원 젤 전기 영동법에 의해 분할되어 졌다. 유의적 발현 수준을 나타낸 많은 단백질 스팟 들은 MALDI-TOP 와 LC-MS/MS에 의해 확인되었으며, 89개의 단백질 스팟 중에 50개의 mass spectra 들이 데이터베이스에서 조류 단백질과 일치함을 확인하였다. 본 실험에서 행한 단백질체 지도는 형질전환 연구와 생식세포 생물학 분야에 중요한 참고 문헌으로 가치를 가질 수 있을 것이다. The domestic chicken (Gallus gallus) is an important model for research in developmental biology because its embryonic development occurs in ovo. To examine the mechanism of embryonic germ cell development, we constructed proteome map of gonadal primordial germ cells (gPGC) from chicken embryonic gonads. Embryonic gonads were collected from 500 embryos at 6 day of incubation, and the gPGC were cultured in vitro until colony formed. After 7-10 days in culture, gPGC colonies were separated from gonadal stroma cells (GSCs). Soluble extracts of cultured gPGCs were then fractionated by two-dimensional gel electrophoresis (pH 4-7). A number of protein spots, including those that displayed significant expression levels, were then identified by use of matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry and LC-MS/MS. Of the 89 gPGC spots examined, 50 yielded mass spectra that matched avian proteins found in on-line databases. Proteome map of thistype will serve as an important reference for germ cell biology and transgenic research.

Clinical outcome of proton therapy for patients with chordomas

Sang Hee Youn,Kwan Ho Cho,Joo-Young Kim,Boram Ha,Young Kyung Lim,Jong Hwi Jeong,Sang Hyun Lee,Heon Yoo,Ho-Shin Gwak,Sang Hoon Shin,Eun Kyung Hong,Han Kyu Kim,Je Beom Hong 대한방사선종양학회 2018 Radiation Oncology Journal Vol.36 No.3

Purpose: To investigate the clinical outcome of proton therapy (PT) in patients with chordoma. Materials and Methods: Fifty-eight patients with chordoma treated with PT between June 2007 and December 2015 at the National Cancer Center, Korea, were retrospectively analyzed. The median total dose was 69.6 cobalt gray equivalent (CGE; range, 64.8 to 79.2 CGE). Local progression-free survival (LPFS), distant metastasis-free survival (DMFS), overall survival (OS), and diseasespecific survival (DSS) rates were calculated by the Kaplan - Meier method. Results: With the median follow-up of 42.8 months (range, 4 to 174 months), the 5-year LPFS, DMFS, OS, and DSS rates were 87.9%, 86.7%, 88.3%, and 92.9%, respectively. The tumor location was associated with the patterns of failure: the LPFS rates were lower for cervical tumors (57.1%) than for non-cervical tumors (93.1%) (p = 0.02), and the DMFS rates were lower for sacral tumors (53.5%) than for non-sacral tumors (100%) (p = 0.001). The total dose was associated with both the LPFS rate and DMFS rate. The initial tumor size was associated with the DMFS rate, but was not associated with the LPFS rate. Three patients had grade 3 late toxicity with none ≥grade 4. Conclusion: PT is an effective and safe treatment in patients with chordomas. The tumor location was associated with the patterns of failure: local failure was common in cervical tumors, and distant failure was common in sacral tumors. Further refinement of PT, such as the utilization of intensity modulated PT for cervical tumors, is warranted to improve the outcome.

Low-dose Ultraviolet A1 Phototherapy for Treating Pityriasis Rosea

( Sang Hee Lim ),( Sang Min Kim ),( Byung Ho Oh ),( Jong Hyun Ko ),( Yang Won Lee ),( Yong Beom Choe ),( Kyu Joong Ahn ) 대한피부과학회 2009 Annals of Dermatology Vol.21 No.3

Background: UVA1 phototherapy has recently demonstrated high levels of efficacy and tolerability for treating a variety of inflammatory and neoplastic skin diseases. Objective: The purpose of the present study was to assess the clinical efficacy of UVA1 (340~400 nm) phototherapy for treating pityriasis rosea and to assess the course of the disease after treatment. Methods: Fifteen patients with extensive pityriasis rosea were treated with low-dose UVA1 phototherapy (starting at 10~20 J/cm2 and then it was increased to 30 J/cm2). The treatments were given 2~3 times a week until complete clearance of lesions was achieved or until there was partial improvement without further amelioration, in spite of 5 additional treatments. The rate of clearing was monitored by estimating the pityriasis rosea severity (PRSS) score and the pruritus score. Results: The extent of disease (PRSS) in all 15 patients lessened during the study (30.1±3.6 vs. 2.0±1.6, respectively, p<0.05). The overall reduction of the PRSS showed a significant improvement after the second or third treatment. The pruritus of 12 of 15 patients lessened during the treatment period, and it was unchanged in the remaining 3 patients. The mean previous duration of disease was 11.2±4.9 days and this did not interfere with the successful outcome of UVA1 phototherapy. Conclusion: This study shows that UVA1 phototherapy is a useful, well-tolerated treatment option for patients suffering from pityriasis rosea with extensive eruptions and considerable pruritus. (Ann Dermatol 21(3) 230~236, 2009)

Characterization of CBF/DREB1 Gene Family in Brassica rapa

Sang-Choon Lee,Myung-Ho Lim,Jae-Gyeong Yu,Beom-Seok Park,Tae-Jin Yang 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

The C-repeat/dehydration-responsive element binding transcription factors (CBF/DREBs) are involved in an important pathway for abiotic stress-response in plants. We have identified CBF/DREB1 gene family from Brassica rapa whole genome sequence and designated them as BrDREB1s. They contain conserved nucleus localization signal, AP2/EREBP domain, and CBF/DREB1 signature, as other known plant CBF/DREB1s. By comparative genomics, we found that nine of ten BrDREB1 genes were present in seven macro-synteny blocks co-linear to four Arabidopsis counterpart blocks and also genomic organizations of their flanking regions were very similar to those for co-linear Arabidopsis CBF/DREB1 genes. In particular, three genes, BrDREB1A, BrDREB1B1, and BrDREB1C1, were closely located within a 59 kb genomic sequence, which was similar to that of their Arabidopsis counterpart genes. However, the genomic regions of those BrDREB1 genes contained additional sequences, compared to their co-linear regions in A. thaliana. The expression of BrDREB1 genes under abiotic stresses were examined by searching microarray database and by RT-PCR analysis. All of eight genes tested were highly up-regulated during cold treatment and some of them were also responsive to salt, drought, and ABA treatment. Taken together, these results indicate that CBF/DREB1-mediated stress signaling pathway is also functioning in B. rapa. On the other hand, differences in genomic organization and gene number for CBF/DREB1 are thought to cause different response to stress between B. rapa and A. thaliana. In this presentation, we will introduce more detailed results for CBF/DREB1 gene family in B. rapa.

Clinical outcome of proton therapy for patients with chordomas

Youn, Sang Hee,Cho, Kwan Ho,Kim, Joo-Young,Ha, Boram,Lim, Young Kyung,Jeong, Jong Hwi,Lee, Sang Hyun,Yoo, Heon,Gwak, Ho-Shin,Shin, Sang Hoon,Hong, Eun Kyung,Kim, Han Kyu,Hong, Je Beom The Korean Society for Radiation Oncology 2018 Radiation Oncology Journal Vol.36 No.3

Purpose: To investigate the clinical outcome of proton therapy (PT) in patients with chordoma. Materials and Methods: Fifty-eight patients with chordoma treated with PT between June 2007 and December 2015 at the National Cancer Center, Korea, were retrospectively analyzed. The median total dose was 69.6 cobalt gray equivalent (CGE; range, 64.8 to 79.2 CGE). Local progression-free survival (LPFS), distant metastasis-free survival (DMFS), overall survival (OS), and disease-specific survival (DSS) rates were calculated by the Kaplan-Meier method. Results: With the median follow-up of 42.8 months (range, 4 to 174 months), the 5-year LPFS, DMFS, OS, and DSS rates were 87.9%, 86.7%, 88.3%, and 92.9%, respectively. The tumor location was associated with the patterns of failure: the LPFS rates were lower for cervical tumors (57.1%) than for non-cervical tumors (93.1%) (p = 0.02), and the DMFS rates were lower for sacral tumors (53.5%) than for non-sacral tumors (100%) (p = 0.001). The total dose was associated with both the LPFS rate and DMFS rate. The initial tumor size was associated with the DMFS rate, but was not associated with the LPFS rate. Three patients had grade 3 late toxicity with none ≥grade 4. Conclusion: PT is an effective and safe treatment in patients with chordomas. The tumor location was associated with the patterns of failure: local failure was common in cervical tumors, and distant failure was common in sacral tumors. Further refinement of PT, such as the utilization of intensity modulated PT for cervical tumors, is warranted to improve the outcome.

취약계층 노동자를 위한 이해대변 조직의 다양성 연구

우상범(Sang Beom Woo),임상훈(Sang Hoon Lim) 한국고용노사관계학회 2021 産業關係硏究 Vol.31 No.3

오랫동안 노동자들의 주요한 이해대변 조직은 노동조합이었다. 최근에는 경영환경 변화로 특수고용직, 플랫폼 노동, 기간제 등 다양한 고용형태를 지닌 취약계층 노동자들이 증가하고 있다. 그러나 이들의 노조조직률이 매우 저조하여 노동조합을 통한 이해대변은 취약한 상황이다. 이에 본 연구는 현재 지역에서 운영되고 있는 비정규지원센터 사례를 분석하여 노조 이외에 취약계층 노동자들의 이해대변 조직의 가능성과 다양성을 제시하려고 한다. 사례 분석 결과 비정규지원센터는 지역의 산업과 노동시장의 특성을 반영하여 광역과 기초 지자체에서 운영되고 있다. 조례를 통해 설립된 비정규지원센터는 지자체의 예산이 투입되고 노사관계 전문성과 경험을 가진 상근자들이 근무하고 있다. 또한, 취약계층 노동자뿐만 아니라 청소년, 일반 시민들을 대상으로 노동인권 교육을 통해 지역사회의 노동 인식 전환을 꾀하고 있다. 무엇보다 비정규지원센터는 노동법률 상담을 통해 법/제도적으로 소외받고 있는 비정규직들의 임금 및 노동조건에 관한 고충을 처리해준다. 이처럼 비정규지원센터는 다양한 사업과 상담 활동으로 취약계층 노동자들의 이해대변 기능을 수행하고 있다. 본 연구는 사례 분석을 통해 비정규지원센터와 노조 간 취약계층 노동자들에 대한 이해대변을 비교했다. 또한, 비정규지원센터가 지닌 이해대변의 가능성과 다양성을 타진하면서 현재의 문제점을 바탕으로 취약계층 이해대변에 대한 시사점과 향후 정책방안을 제시했다. For a long time, primary interest representation organization for workers is trade union. Recently, it increases a lot of forms of employment like irregular worker, platform labor because of change of management environment. Their interest representation, however, is weak for their low union density. Therefore, this paper proposes possibility and diversity of interest representation organizations for vulnerable workers through cases of 4 contingent worker support centers. Centers are managed in the local government based on local industry and labor market and the local government supports the centers founded by ordinance with local budget. Many full-time employees show their career and experience for workers’ support in the centers. The centers contribute change of labor cognition for vulnerable workers, the young and citizen through labor education in local community. Most of all, the centers solve difficulties such as wage, working conditions and unfair dismissal for irregular workers by expert labor/legal advice. This paper analyzed interest representation between the centers and trade union and showed possibility and diversity of interest representation organization. Finally, this article proposed theoretical implications and policy plan for interest representation of vulnerable workers.

한국인 피부에서 분리된 Malassezia dermatis의 진균 및 분자생물학적 고찰

임상희 ( Sang Hee Lim ),김상민 ( Sang Min Kim ),정보라 ( Bo Ra Jung ),이양원 ( Yang Won Lee ),최용범 ( Yong Beom Choe ),안규중 ( Kyu Joong Ahn ) 대한피부과학회 2007 대한피부과학회지 Vol.45 No.10

Background: Malassezia (M.) yeasts are lipophilic fungi which are regarded as normal flora of the skin, and are recovered in 75∼98% of healthy adults. Gueho et al reclassified the Malassezia yeasts into 7 species (M. furfur, M. obtusa, M. globosa, M. slooffiae, M. sympodialis, M. pachydermatis, M. restricta) on the basis of molecular biology and by employing an interdisciplinary approach of morphology, microstructurology and physiology. Recently novel species of the genus Malassezia have been discovered as a result of molecular analysis. But there are no additional reports in Korea regarding newly reported Malassezia species because most identification and classification of Malassezia in Korea depend on classical methods and research on molecular biologic application is insufficient. Objective: Five clinical isolates of M. dermatis were isolated from the skin of healthy subjects without skin disease or seborrheic dermatitis patients using molecular biology techniques for the first time in Korea. Hence the present study describes mycological and molecular biological characteristics of these five isolates as a novel species of M. dermatis. Methods: Morphological and biochemical analyses, such as colony morphologies, microscopic morphologies and physiological characteristic were done targeting 5 clinical isolates of M. dermatis. Molecular techniques, namely, 26S rDNA PCR-RFLP, 26S rDNA and internal transcribed spacer region 1 (ITS1) sequencing, were done for identification and phylogenetic systematic analysis. Results: Five clinical isolates of M. dermatis showed positive in the catalase test. No growth is obtained on Sabouraud`s dextrose agar (SDA) without lipid supplementation but all grew in 0.5% Tween 60 and 0.1% Tween 80 added 2% glucose/1% peptone culture medium. Round and ellipsoidal yeast cells and budding of the yeast cells were observed under microscope, resembling M. sympodialis, M. furfur, and M. nana. The 26S rDNA PCR-RFLP pattern showed the same pattern as M. dermatis (JCM 11348), the standard strain. 26S rDNA and ITS1 sequencing were performed for exact identification, showing 99% accordance with M. dermatis (AB070361), and M. dermatis (AB070356), confirming the species to be new, the first to be reported in Korea. Phylogenetic trees based on the D1/D2 domains of the 26S rDNA sequences and nucleotide sequences of the ITS 1 region showed that the isolates were conspecific and belonged to the genus Malassezia and crusted with M. sympodialis. Conclusion: Taking a molecular biological classification approach, we have successfully isolated 5 cases of M. dermatis-the first in Korea. Although it is not known whether M. dermatis plays a role in Malassezia-related skin disease, this species was part of the microflora in both patients with seborrheic dermatitis and healthy subjects. (Korean J Dermatol 2007;45(10):1020∼1030)