Gastroprotective and gastric motility benefits of AD-lico/Healthy Gut™ Glycyrrhiza inflata extract

ALISTARE ROBERT SADR,조제영,허성오,권혁세 한국통합생물학회 2017 Animal cells and systems Vol.21 No.4

The aim of this study was to evaluate in vivo both the anti-Helicobacter and the gastric-relaxing effects of AD-lico/Healthy Gut™ in rat models. AD-lico/Healthy Gut™ is a specially prepared commercial formulation of Glycyrrhiza inflata extract that is under clinical development for indications of gastrointestinal disease and inflammatory bowel disease. In the current study, the oral administration of AD-lico/Healthy Gut™ significantly reduced mucosal damage from Helicobacter pylori in rats and decreased the expression of the inflammatory markers iNOS and COX-2 in the test cells. AD-lico/Healthy Gut™ also reduced mucosal damage caused by water immersion stress in rats. The accelerated gastric emptying in normal rats was also seen with ADlico/ Healthy Gut™, providing relief in gastric relaxation in the test animals. The special formulation of AD-lico/Healthy Gut™ with reduced levels of component glycyrrhizin also has benefits in minimizing the potential side effects attributed to glycyrrhizin seen with similar Glycyrrhiza extracts in terms of induction of hypokalemia and muscle weakness. The preparation has a relatively high phenolic compound content relative to other methods of preparation and is indicative of lower glycyrrhizin levels. These results suggest that AD-lico/Healthy Gut™ may provide the necessary relief from a number of stomach discomfort issues faced by a large population of people.

Synthesis and structural characterization of three dinuclear Copper(II) complexes incorporating pyrazolyl-derived ligands

Soltani, Behzad,Sadr, Moayad Hossaini,Engle, James T.,Ziegler, Christopher J.,Joo, Sang Woo,Hanifehpour, Younes Springer-Verlag 2012 Transition metal chemistry Vol.37 No.8

Embryoid Body Size-Mediated Differential Endodermal and Mesodermal Differentiation Using Polyethylene Glycol (PEG) Microwell Array

차재민,황유식,배호재,Nasser Sadr,Sam Manoucheri,Faramarz Edalat,김기영,김상복,권일근,ALI KHADEMHOSSEINI 한국고분자학회 2015 Macromolecular Research Vol.23 No.3

Embryoid bodies have a number of similarities with cells in gastrulation, which provides useful biologicalinformation about embryonic stem cell differentiation. Extensive research has been done to study the control ofembryoid body-mediated embryonic stem cell differentiation in various research fields. Recently, microengineeringtechnology has been used to control the size of embryoid bodies and to direct lineage specific differentiation ofembryonic stem cells. However, the underlying biology of developmental events in the embryoid bodies of differentsizes has not been well elucidated. In this study, embryoid bodies with different sizes were generated within microfabricatedPEG microwell arrays, and a series of gene and molecular expressions related to early developmentalevents was investigated to further elucidate the size-mediated differentiation. The gene and molecular expressionprofile suggested preferential visceral endoderm formation in 450 μm embryoid bodies and preferential lateral platemesoderm formation in 150 μm embryoid bodies. These aggregates resulted in higher cardiac differentiation in 450 μmembryoid bodies and higher endothelial differentiation in 150 μm embryoid bodies, respectively. Our findings mayprovide further insight for understanding embryoid body size-mediated developmental progress.

Delamination growth analysis in composite laminates subjected to low velocity impact

Masoud Kharazan,M.H. Sadr,Morteza Kiani 국제구조공학회 2014 Steel and Composite Structures, An International J Vol.17 No.4

This paper presents a high accuracy Finite Element approach for delamination modelling in laminated composite structures. This approach uses multi-layered shell element and cohesive zone modelling to handle the mechanical properties and damages characteristics of a laminated composite plate under low velocity impact. Both intralaminar and interlaminar failure modes, which are usually observed in laminated composite materials under impact loading, were addressed. The detail of modelling, energy absorption mechanisms, and comparison of simulation results with experimental test data were discussed in detail. The presented approach was applied for various models and simulation time was found remarkably inexpensive. In addition, the results were found to be in good agreement with the corresponding results of experimental data. Considering simulation time and results accuracy, this approach addresses an efficient technique for delamination modelling, and it could be followed by other researchers for damage analysis of laminated composite material structures subjected to dynamic impact loading.

Farnesylation-defective Rheb Increases Axonal Length Independently of mTORC1 Activity in Embryonic Primary Neurons

최승혁,ALISTARE ROBERT SADR,강지은,류재련,김준환,선웅,허성오 한국뇌신경과학회 2019 Experimental Neurobiology Vol.28 No.2

Rheb (Ras homolog enriched in the brain) is a small GTPase protein that plays an important role in cell signaling for development of the neocortex through modulation of mTORC1 (mammalian-target-of-rapamycin-complex-1) activity. mTORC1 is known to control various biological processes including axonal growth in forming complexes at the lysosomal membrane compartment. As such, anchoring of Rheb on the lysosomal membrane via the farnesylation of Rheb at its cysteine residue (C180) is required for its promotion of mTOR activity. To test the significance of Rheb farnesylation, we overexpressed a farnesylation mutant form of Rheb, Rheb C180S, in primary rat hippocampal neurons and also in mouse embryonic neurons using in utero electroporation. Interestingly, we found that Rheb C180S maintained promotional effect of axonal elongation similar to the wild-type Rheb in both test systems. On the other hand, Rheb C180S failed to exhibit the multiple axon-promoting effect which is found in wild-type Rheb. The levels of phospho-4EBP1, a downstream target of mTORC1, were surprisingly increased in Rheb C180S transfected neurons, despite the levels of phosphorylated mTOR being significantly decreased compared to control vector transfectants. A specific mTORC1 inhibitor, rapamycin, also could not completely abolish axon elongation characteristics of Rheb C180S in transfected cells. Our data suggests that Rheb in a non-membrane compartment can promote the axonal elongation via phosphorylation of 4EBP1 and through an mTORC1-independent pathway.

The combined effect of dismantling for steam sterilization and aging on the accuracy of spring-style mechanical torque devices

Mahshid, Minoo,Saboury, Aboulfazl,Sadr, Seyed Jalil,Fayyaz, Ali,Kadkhodazadeh, Mahdi Korean Academy of Periodontology 2013 Journal of Periodontal & Implant Science Vol.43 No.5

Purpose: This study aimed to assess the combined effect of dismantling before sterilization and aging on the accuracy (${\pm}10%$ of the target torque) of spring-style mechanical torque devices (S-S MTDs). Methods: Twenty new S-SMTDs from two different manufacturers (Nobel Biocare and Straumann: 10 of each type) were selected and divided into two groups, namely, case (group A) and control (group B). For sterilization, 100 cycles of autoclaving were performed in 100 sequences. In each sequence, 10 repetitions of peak torque values were registered for aging. To measure and assess the output of each device, a Tohnichi torque gauge was used (P<0.05). Results: Before steam sterilization, all of the tested devices stayed within 10% of their target values. After 100 cycles of steam sterilization and aging with or without dismantling of the devices, the Nobel Biocare devices stayed within 10% of their target torque. In the Straumann devices, despite the significant difference between the peak torque and target torque values, the absolute error values stayed within 10% of their target torque. Conclusion: Within the limitations of this study, there was no significant difference between the mean and absolute value of error between Nobel Biocare and Straumann S-S MTDs.

A modified multi-objective elitist-artificial bee colony algorithm for optimization of smart FML panels

Ghashochi-Bargha, H.,Sadr, M.H. Techno-Press 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.52 No.6

In Current paper, the voltages of patches optimization are carried out for minimizing the power consumption of piezoelectric patches and maximum vertical displacement of symmetrically FML panels using the modified multi-objective Elitist-Artificial Bee Colony (E-ABC) algorithm. The voltages of patches, panel length/width ratios, ply angles, thickness of metal sheets and edge conditions are chosen as design variables. The classical laminated plate theory (CLPT) is considered to model the transient response of the panel, and numerical results are obtained by the finite element method. The performance of the E-ABC is also compared with the PSO algorithm and shows the good efficiency of the E-ABC algorithm. To check the validity, the transient responses of isotropic and orthotropic panels are compared with those available in the literature and show a good agreement.

PSO algorithm for fundamental frequency optimization of fiber metal laminated panels

Ghashochi-Bargh, H.,Sadr, M.H. Techno-Press 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.47 No.5

In current study, natural frequency response of fiber metal laminated (FML) fibrous composite panels is optimized under different combination of the three classical boundary conditions using particle swarm optimization (PSO) algorithm and finite strip method (FSM). The ply angles, numbers of layers, panel length/width ratios, edge conditions and thickness of metal sheets are chosen as design variables. The formulation of the panel is based on the classical laminated plate theory (CLPT), and numerical results are obtained by the semi-analytical finite strip method. The superiority of the PSO algorithm is demonstrated by comparing with the simple genetic algorithm.

Augmented reduction in colonic inflammatory markers of dextran sulfate sodiuminduced colitis with a combination of 5-aminosalicylic acid and AD-lico™ from Glycyrrhiza inflata

조재영,권혁세,허성오,ALISTARE ROBERT SADR 한국통합생물학회 2018 Animal cells and systems Vol.22 No.3

The primary aim of this study was to determine whether the oral administration of AD-lico™, a functional extract from Glycyrrhiza inflata in combination with 5-aminosalicylic acid (5-ASA) could ameliorate the inflammatory symptoms in dextran sulfate sodium (DSS)-induced colitis in rodents. This DSS rodent model is used to study drug candidates for colitis, as part of the spectrum of diseases falling under the inflammatory bowel disease (IBD) category. Here, with oral AD-lico™ administration, there was a substantial disruption of the colonic architectural changes due to DSS and a significant reduction in colonic myeloperoxidase (MPO) activity, a marker of colitis. In the same samples, there were also reduced levels of colonic and serum IL-6 in the oral AD-lico™ treated rats. This study also addressed the possible mechanisms for ADlico ™ mediated changes on colonic inflammation markers. These included the observations that AD-lico™ dampened the IL-6 proinflammatory-signaling pathway in THP-1 human monocytic cells and reduced the TNFα-mediated upregulation of surface adhesion molecule ICAM-1 in human umbilical vein endothelial cells (HUVECs). Finally, it was shown that AD-lico™ could be combined with 5-ASA in reducing the inflammatory markers for colorectal sites affected by colitis, a first study of its kind for a combination therapy.

PSO algorithm for fundamental frequency optimization of fiber metal laminated panels

H. Ghashochi-Bargh,M.H. Sadr 국제구조공학회 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.47 No.5

In current study, natural frequency response of fiber metal laminated (FML) fibrous composite panels is optimized under different combination of the three classical boundary conditions using particle swarm optimization (PSO) algorithm and finite strip method (FSM). The ply angles, numbers of layers, panel length/width ratios, edge conditions and thickness of metal sheets are chosen as design variables. The formulation of the panel is based on the classical laminated plate theory (CLPT), and numerical results are obtained by the semi-analytical finite strip method. The superiority of the PSO algorithm is demonstrated by comparing with the simple genetic algorithm.