유치원 敎師職의 專門性에 관한 考察

盧公根 論文集 1982 論文集 Vol.3 No.-

The necessity of Kindergarten education is rising to the high tide as we realize the importance of infants education. In kindergarten teachers ability is largely stressed so that the teaching could be done effectively. In this study, Professional characteristics of kindergasten teachers are cleared to set up the true image of kindergarten educators. Distribution of guestionaire to kindergarten teachers who are in the first line and research on related booklets were used simultaneously for this study. Questionairs distributed dates were Oct. 1 - 20, 1980. Recovered guestionaires were 210 and showed collected rate of 60 percent. In handling the statistics, response was conversed into percentage and occasionally weight secure was given to get precedence. Facts obtained from the Study: 1. Professional Characteristics can be embodied, which help emphasize the unique roles of kindergarten teachers. 2. Relay of childrens socialization is rather a heavier role of kindergarten teachers than to give the necessary lessons (explicit curriculum) taught in kindergarten. 3. Factors preventing those distinctive features of teaching in kindergarten are destined, in general derived from social, systematic problems and also from conventionality. 4. Many of kindergarten teachers become incidentaily, however they come to admit the importance of infants, education through direct experience and tend to hold the job even though they are paid low. 5. A large portion of 83 percent among kindergarten teachers tell "Payment is low" and feel that "they are not respected from the society." These make the job unattractive all together. 6. However, they have an optimistic view about kindergarten education (10 years later) which could be developed mainly by payment increase ans training qualified teachers.

Ogive-Cylinder 형 첨두부를 갖는 유도무기 주위 고앙각 흐름에서의 비대칭 와류 특성과 이에 따른 측력발생에 대한 수치적 해석 연구

정성기,제상언,명노신,조태환 慶尙大學校 經營行政大學院 2004 工學硏究院論文集 Vol.20 No.-

The demand for continually increased performance of missiles and aircraft leads to considering flight at very high angle of attack where control is very difficult. This is mainly due to shedding of asymmetric vortices from the forebody, producing side forces even at zero sideslip. In this paper, Computational Fluid Dynamics(CFD) was used to analyze characteristic of asymmetric vortices and examine the effects of several cases for rising asymmetric vortices. There are asymmetric grid cluster, sideslip angle and roughness. As a result of the study, asymmetric vortices were sensitive at the areas of roughness and the analysis results coincided with the experiment results at the specified area.

Detection of Hepatitis B virus DNA in Human Serum by Dot Hybridization Using a Biotin-Labelled Probe

Im Hwan Roe,Jung Hye Roe,Dong Hoo Lee 대한내과학회 1988 The Korean Journal of Internal Medicine Vol.3 No.1

Biotin 표지 HBV DNA 를 이용한 Dot Hybridization 에 관한 연구

노임환(Im Hwan Roe),이동후(Jung Hye Roe),노정혜(Dong Hoo Lee) 대한소화기학회 1988 대한소화기학회지 Vol.20 No.3

N/A A dot blot hybridization technique utilizing a biotin-labelled recombinant DNA probe was used to examine hepatitis B virus (HBV) DNA in serum. The lowest amount of HBV DNA in serum detectable by the color development of an avidin-biotin alkaline phosphatase complex was 40 picogram per 50 microliter. Validity of this method was confirmed by autoradiography using 32P-labelled and 3H-labelled HBV DNA probes. HBV DNA was found in 100% (34/34) of the HBsAg-positive and in 73.5% (25/34) of the HBsAg-negative subjects. In contrast, all nine cases showing negativity in HBV DNA were also HBsAg-negative. Correlation of Hbe antigen/antibody with HBV DNA was investigated in 19 sera of which HBsAg was negative but anti-HBc positive. Of 13 sera with anti-Hbe 10 (76.9%) cases revealed HBV DNA positivity, while four (66.7%) of six sera without anti-HBe were positive in HBV DNA. In conclusion, serum dot hybridization assay utilizing a bir>tinylated probe proved useful in the detection of a free form of HBV DNA regardless of the presence of HBsAg and irrespective of HBeAg-anti-HBe status. Also an anti-HBs does not always correspond to the clearance of HBV. And this indicates, in turn, that the potential infectivity of certain sera previously considered to be safe should be kept in mind. Moreover, it is emphasized that practical advantages in speed, reproducibility, and safety have made this alternative even more attractive than autoradiography using radioisotope- labelled probes.

The Positive Regulation of p53 by the Tumor Suppressor VHL

Roe, Jae-Seok,Youn, Hong-Duk Informa UK (TaylorFrancis) 2006 Cell cycle Vol.5 No.18

<P>The ubiquitin-mediated degradation of hypoxia-inducible factor-alpha (HIF-alpha) by a von Hippel-Lindau tumor suppressor protein (pVHL) is mechanistically responsible for controlling gene expression due to oxygen availability. Germline mutations in the VHL gene cause dysregulation of HIF and induce an autosomal dominant cancer syndrome referred to as VHL disease. However, it is unclear whether HIF accumulation caused by VHL mutations is sufficient for tumorigenesis. Recently, we found that pVHL directly associates and positively regulates the tumor suppressor p53 by inhibiting Mdm2-mediated ubiquitination, and by subsequently recruiting p53-modifying enzymes. Moreover, VHL-deleted RCC cells showed attenuated apoptosis or abnormal cell-cycle arrest upon DNA damage, but became normal when pVHL was restored. Thus, pVHL appears to play a pivotal role in tumor suppression by participating actively as a component of p53 transactivation complex during DNA damage response.</P>

Phosphorylation of von Hippel-Lindau protein by checkpoint kinase 2 regulates p53 transactivation.

Roe, Jae-Seok,Kim, Hwa-Ryeon,Hwang, In-Young,Ha, Nam-Chul,Kim, Seong-Tae,Cho, Eun-Jung,Youn, Hong-Duk Landes Bioscience 2011 Cell Cycle Vol.10 No.22

<P>von-Hippel Lindau protein (pVHL) suppresses tumorigenesis in the kidney, in part through regulation of hypoxia-inducible factor alpha (HIF alpha). However, HIF has been proposed to be necessary but insufficient for renal tumorigenesis. p53 was implicated as a transcription factor that is regulated by pVHL, but the molecular mechanism by which pVHL regulates p53 on DNA damage is unknown. We demonstrated that checkpoint kinase-2 (Chk2) binds to the beta-domain of pVHL and phosphorylates Ser 111 on DNA damage. Notably, this modification enhances pVHL-mediated transactivation of p53 by recruiting p300 and Tip60 to the chromatin of p53 target gene. Further, the naturally occurring pVHL mutants pVHL-S111R and pVHL-S111C showed diminished binding to coactivators, ultimately retarding p53-mediated growth arrest and apoptosis. In this study, we determined the molecular mechanism by which pVHL transactivates p53 on DNA damage and demonstrated that p53-related pVHL subtype mutants regulate tumorigenecity in VHL diseases.</P>

The Late Transcripts of SV40

Roe, Jung Hye,Park, Joo Sang 한국유전학회 1988 Genes & Genomics Vol.10 No.4

We have examined the structures and cellulardistributions of the SV40 late RNAs present in monkey cells at late times after infection. One particular RNA species, spliced at residue 373 (373-RNA), was found to be as abundant as the major late 16S RNAs. This result was unexpected since previous reports showed that the molecular ratio of the 373-spliced 19S RNA to 16S RNA is approximately 0.1 among either cytoplasmic polyadenylated or polysomal viral RNAs. Both sedimentation and electrophoretic analyses indicated that the 373-RNA was approximately 16S to 19S in size. Analysis of the structure of this novel RNA species by S1 mapping and primer extension showed that its 5′ end mapped to the major cap site at nucleotide residue 325 and that nucleotide residue 373 was joined covalently to nucleotide residue 558 as is the case with the majority of the cytoplasmic polyadenylated SV40 late 19S RNAs. However, whereas most SV40 late 16S RNA is polyadenylated and located in the cytoplasm, the majority of 373-RNA was found to lack polyA, exhibit sequence heterogeneity at its 3′ end and be located in the nucleus. The cellular distribution is reversed when the remaining 3′ splice region (around residue 1463) is deleted from this RNA. This implies that the 3' splice region, in the absence of its 5′ splice pair, inhibits the nuclear transport of this 19S spliced RNA, possibly by forming a splicing complex which is an intermediate for the formation of a complete spliceosome.

The Characterization of an Abundant SV40 Late RNA Species in the Nucleus of the Infected Monkey Cell

Roe, Jung Hye 한국유전학회 1986 Genes & Genomics Vol.8 No.4

Late in the lytic cycle of infection of monkey cells SV40 produces two classes of late RNAs, 16S and 19S in size, which encode the virion proteins VP1 and VP2/3, respectively. These RNAs are made by differential splicing of the primary nuclear transcripts and exhibit heterogeneity in their 5'ends as well as splice sites utilized in processing. The mechanism of selection is not yet known. The relative abundances of the various species of spliced late RNAs present in the cytoplasm of SV40-infected monkey cells has been determined for both polysomal and polyadenylated RNA. The ratio of 16S to 19S cytoplasmic, polyadenylated RNA is approximately four. I have examined the structures and cellular distributions of the SV40 late RNAs present in monkey cells at late times after infection (48 hours after infection). One particular RNA species, spliced at residue 373 (373-RNA), was found to be as abundant as the major late 16S RNAs. This result was unexpected since previous reports showed that the molecular ratio of the 373-spliced 19S RNA to 16S RNA is approximately 0.1 among either cytoplasmic polyadenylated or polysomal viral RNAs. Both sedimentation and electrophoretic analyses indicated that the 373-RNA was approximately 16S to 19S in size. Therefore, it was not a splicing intermediate or the product of premature termination of transcription within the late leader region. Analysis of the structure of this RNA by S1 mapping and primer extension showed that its 5'end mapped to the major cap site (at nt. 325) and that nucleotide residue 373 was joined to residue 558 as is found in the majority of the cytoplasmic, polyadenylated SV40 late 19S RNAs. However, as judged by the oligo (dT)-cellulose chromatography, whereas most SV40 late 16S RNA is polyadenylated and located in the cytoplasm, the majority of 373-RNA was found to lack poly A tail, exhibit heterogeneity at its 3'end and be located in the nucleus. It is probable that the lack of poly A caused the preferential distribution of 373-RNA in the nucleus, thus regulating the amount of the functional mRNA in the cytoplasm. The structure of the 3'end of this RNA is currently under investigation. This will tell whether the 3'end is generated by transcriptional termination or by random degradation. Whether this RNA has ever been polyadenylated will be determined by the pulse-chase type of experiments.

p53 STABILIZATION AND TRANSACTIVATION BY A VON HIPPEL-LINDAU PROTEIN

Roe, Jae-Seok,Kim, Hyungsoo,Lee, Soon-Min,Youn, Hong-Duk 이화여자대학교 세포신호전달연구센터 2006 고사리 세포신호전달 심포지움 Vol. No.8

von Hippel-Lindau(VHL) disease is a rare autosomal dominant cancer syndrome. Although hypoxia inducible factor-α(HIF-α) is a well-documented substrate of a von Hippel-Lindau tumor suppressor protein(pVHL), it remains controversial as to whether the dysregulation of HIF is sufficient for de novo tumorigenesis in VHL-deleted cells, which suggests that pVHL may have HIF-independent tumor suppressor functions. Here we found that pVHL directly associates with and stabilizes p53 by suppressing the mdm2-mediated ubiquitination and nuclear export of p53. Moreover, upon genotoxic stress, pVHL invoked a synergistic interaction between p53 and p300, and thus the acetylation of p53, which ultimately led to increasing p53 transcriptional activity and p53-mediated cell cycle arrest and apoptosis. This mechanism suggests that the tumor suppressor pVHL has an unexpected function to upregulate another tumor suppressor p53.

Changing Geopolitical Boundaries and their Impact upon Port Selection and Concentration

ROE, MiChael 한국해운물류학회 2004 해운물류연구 Vol.43 No.-

There have been substantial geopolitical changes world-wide in the past twenty years which have led to the creation of new countries, the re-emergence of old ones, and a number of serious border movements. Examples include the emergence of Slovenia, Russia and Moldova as separate national identities, the reunification of Germany from the old Federal and Democratic Republics, and the creation of Kazahkstan, Armenia and Georgia. The future holds the prospect of further change including the potential for re-unification of Korea. In each case the effect upon transport and logistics has been substantial and within this, there has occurred a large number of major changes in port choice, commodities carried, direction of trade and mode. In turn these changes have had a major impact upon port selection and concentration. This paper examines the role of geopolitical change and its relationship to ports and analyses a model of the changes that have occurred in recent years concentrating upon the port impacts. Case studies from Eastern Europe and Central Asia will be used to develop the analysis whist a concluding section will examine the potential for further geopolitical/port inter-relationships in the context of developments in Korea, the Trans-Siberian Railway and the Far East-European market.