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RISS 인기검색어
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- 저자 : 이동후(Jung Hye Roe) (검색결과 1 건)
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Biotin 표지 HBV DNA 를 이용한 Dot Hybridization 에 관한 연구
노임환(Im Hwan Roe),이동후(Jung Hye Roe),노정혜(Dong Hoo Lee) 대한소화기학회 1988 대한소화기학회지 Vol.20 No.3
N/A A dot blot hybridization technique utilizing a biotin-labelled recombinant DNA probe was used to examine hepatitis B virus (HBV) DNA in serum. The lowest amount of HBV DNA in serum detectable by the color development of an avidin-biotin alkaline phosphatase complex was 40 picogram per 50 microliter. Validity of this method was confirmed by autoradiography using 32P-labelled and 3H-labelled HBV DNA probes. HBV DNA was found in 100% (34/34) of the HBsAg-positive and in 73.5% (25/34) of the HBsAg-negative subjects. In contrast, all nine cases showing negativity in HBV DNA were also HBsAg-negative. Correlation of Hbe antigen/antibody with HBV DNA was investigated in 19 sera of which HBsAg was negative but anti-HBc positive. Of 13 sera with anti-Hbe 10 (76.9%) cases revealed HBV DNA positivity, while four (66.7%) of six sera without anti-HBe were positive in HBV DNA. In conclusion, serum dot hybridization assay utilizing a bir>tinylated probe proved useful in the detection of a free form of HBV DNA regardless of the presence of HBsAg and irrespective of HBeAg-anti-HBe status. Also an anti-HBs does not always correspond to the clearance of HBV. And this indicates, in turn, that the potential infectivity of certain sera previously considered to be safe should be kept in mind. Moreover, it is emphasized that practical advantages in speed, reproducibility, and safety have made this alternative even more attractive than autoradiography using radioisotope- labelled probes.
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