Identification of loci and genes for growth related traits from a genome-wide association study in a slow- 3 fast-growing broiler chicken cross

Ranran Liu,Yanfa Sun,Guiping Zhao,Hongyang Wang,Maiqing Zheng,Peng Li,Li Liu,Jie Wen 한국유전학회 2015 Genes & Genomics Vol.37 No.10

Identification of functional genes for multifactorial traits is an ongoing priority. A genome-wide association study and gene expression study for weight and carcass composition traits is presented here using a F2 resource population derived from a cross between slowand fast-growing chicken breeds. For weight traits, eight SNPs on GGA4 plus one SNP on GGA27 were significantly associated with two or more target traits, including body weight (BW) at day 56, BW93, carcass weight, eviscerated weight, breast weight (BrW), thigh muscle weight (ThW) and wing weight. Of 11candidate genes identified near significant SNPs, three were shown by Q-PCR to be differentially expressed in breast muscle between chickens selected from phenotypic extremes of carcass weight and BrW, including ligand dependent nuclear receptor corepressor-like protein and LIM domainbinding factor 2. For major carcass composition traits, nine significant SNPs on GGA1, GGAZ and GGA6 were identified as being associated with BrW and/or percentage. The wings apart-like homolog (Drosophila, WAPAL) on GGA6 had the most significant association with percentage of ThW.

Identification of Histone Deacetylase 2 as a Functional Gene for Skeletal Muscle Development in Chickens

Shahjahan, Md.,Liu, Ranran,Zhao, Guiping,Wang, Fangjie,Zheng, Maiqing,Zhang, Jingjing,Song, Jiao,Wen, Jie Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.4

A previous genome-wide association study (GWAS) exposed histone deacetylase 2 (HDAC2) as a possible candidate gene for breast muscle weight in chickens. The present research has examined the possible role of HDAC2 in skeletal muscle development in chickens. Gene expression was measured by quantitative polymerase chain reaction in breast and thigh muscles during both embryonic (four ages) and post-hatch (five ages) development and in cultures of primary myoblasts during both proliferation and differentiation. The expression of HDAC2 increased significantly across embryonic days (ED) in breast (ED 14, 16, 18, and 21) and thigh (ED 14 and 18, and ED 14 and 21) muscles suggesting that it possibly plays a role in myoblast hyperplasia in both breast and thigh muscles. Transcript abundance of HDAC2 identified significantly higher in fast growing muscle than slow growing in chickens at d 90 of age. Expression of HDAC2 during myoblast proliferation in vitro declined between 24 h and 48 h when expression of the marker gene paired box 7 (PAX7) increased and cell numbers increased throughout 72 h of culture. During induced differentiation of myoblasts to myotubes, the abundance of HDAC2 and the marker gene myogenic differentiation 1 (MYOD1), both increased significantly. Taken together, it is suggested that HDAC2 is most likely involved in a suppressive fashion in myoblast proliferation and may play a positive role in myoblast differentiation. The present results confirm the suggestion that HDAC2 is a functional gene for pre-hatch and post-hatch (fast growing muscle) development of chicken skeletal muscle.

Developmental Hypothyroidism Influences the Development of the Entorhinal-Dentate Gyrus Pathway of Rat Offspring

Ting Jin,Ranran Wang,Shiqiao Peng,Xin Liu,Hanyi Zhang,Xue He,Weiping Teng,Xiaochun Teng 대한내분비학회 2022 Endocrinology and metabolism Vol.37 No.2

Background: Developmental hypothyroidism impairs learning and memory in offspring, which depend on extensive neuronal circuits in the entorhinal cortex, together with the hippocampus and neocortex. The entorhinal-dentate gyrus pathway is the main entrance of memory circuits. We investigated whether developmental hypothyroidism impaired the morphological development of theentorhinal-dentate gyrus pathway. Methods: We examined the structure and function of the entorhinal-dentate gyrus pathway in response to developmental hypothyroidism induced using 2-mercapto-1-methylimidazole. Results: 1,1´-Dioctadecyl-3,3,3´,3´-tetramethylindocarbocyanine perchlorate tract tracing indicated that entorhinal axons showeddelayed growth in reaching the outer molecular layer of the dentate gyrus at postnatal days 2 and 4 in hypothyroid conditions. Theproportion of fibers in the outer molecular layer was significantly smaller in the hypothyroid group than in the euthyroid group atpostnatal day 4. At postnatal day 10, the pathway showed a layer-specific distribution in the outer molecular layer, similar to the euthyroid group. However, the projected area of entorhinal axons was smaller in the hypothyroid group than in the euthyroid group. Anelectrophysiological examination showed that hypothyroidism impaired the long-term potentiation of the perforant and the cornuammonis 3–cornu ammonis 1 pathways. Many repulsive axon guidance molecules were involved in the formation of the entorhinaldentate gyrus pathway. The hypothyroid group had higher levels of erythropoietin-producing hepatocyte ligand A3 and semaphorin3A than the euthyroid group. Conclusion: We demonstrated that developmental hypothyroidism might influence the development of the entorhinal-dentate gyruspathway, contributing to impaired long-term potentiation. These findings improve our understanding of neural mechanisms formemory function.

A review on the risk, prevention and control of cooling water intake blockage in coastal nuclear power plants

Heshan Lin,Shuyi Zhang,Ranran Cao,Shihao Yu,Wei Bai,Rongyong Zhang,Jia Yang,Li Dai,Jianxin Chen,Yu Zhang,Hongni Xu,Kun Liu,Xinke Zhang Korean Nuclear Society 2024 Nuclear Engineering and Technology Vol.56 No.2

In recent decades, numerous instances of blockages have been reported in coastal nuclear power plants globally, leading to serious safety accidents such as power reduction, manual or automatic power loss, or shutdown of nuclear power units. Loss or shortage of cooling water may compromise the reliability of the cooling water system, thus threatening the operational safety of power plants and resulting in revenue reduction. This study provides a comprehensive review of the current state of cooling water system safety in coastal nuclear power plants worldwide and the common challenges they face, as well as the relevant research on cooling water system safety issues. The research overview and progress in investigation methods, outbreak mechanisms, prevention and control measures, and practical cases of blockages were summarized. Despite existing research, there are still many shortcomings regarding the pertinence, comprehensiveness and prospects of related research, and many problems urgently need to be solved. The most fundamental concern involves understanding the list of potential risks of blockages and their spatially distributed effects in surrounding waters. Furthermore, knowledge of the biological cycles and ecological habits of key organisms is essential for implementing risk prevention and control and for building a scientific and effective monitoring system.

Polymorphisms of Ionotropic Glutamate Receptor-Related Genes and the Risk of Autism Spectrum Disorder in a Chinese Population

Xinyan Xie,Fang Hou,Li Li,Yanlin Chen,Lingfei Liu,Xiu Luo1,Huaiting Gu,Xin Li,Jiajia Zhang,Jianhua Gong,Ranran Song 대한신경정신의학회 2019 PSYCHIATRY INVESTIGATION Vol.16 No.5

Objective: To evaluate the association of GRIK2 and NLGN1 with autism spectrum disorder in a Chinese population. Methods: We performed spatio-temporal expression analysis of GRIK2 and NLGN1 in the developing prefrontal cortex, and examined the expression of the genes in ASD cases and healthy controls using the GSE38322 data set. Following, we performed a case-control study in a Chinese population. Results: The analysis using the publicly available expression data showed that GRIK2 and NLGN1 may have a role in the development of human brain and contribute to the risk of ASD. Later genetic analysis in the Chinese population showed that the GRIK2 rs6922753 for the T allele, TC genotype and dominant model played a significant protective role in ASD susceptibility (respectively: OR=0.840, p=0.023; OR=0.802, p=0.038; OR=0.791, p=0.020). The NLGN1 rs9855544 for the G allele and GG genotype played a significant protective role in ASD susceptibility (respectively: OR=0.844, p=0.019; OR=0.717, p=0.022). After adjusting p values, the statistical significance was lost (p>0.05). Conclusion: Our results suggested that GRIK2 rs6922753 and NLGN1 rs9855544 might not confer susceptibility to ASD in the Chinese population.