Polymorphisms in TYMS for Prediction of Capecitabine-Induced Hand-Foot Syndrome in Chinese Patients with Colorectal Cancer

Si-Qi Dong,Tong-Min Wang,Jiang-Bo Zhang,Yong-Qiao He,Wen-Qiong Xue,Zi-Yi Wu,Da-Wei Yang,Lian-Jing Cao,Jing-Wen Huang,Xi-Zhao Li,Pei-Fen Zhang,Xiao-Hui Zheng,Wei-Hua Jia 대한암학회 2021 Cancer Research and Treatment Vol.53 No.3

Purpose Capecitabine is an extensively used oral prodrug of 5-fluorouracil in treatment of colon cancer and is known to cause hand-foot syndrome (HFS). As the target enzyme for capecitabine, thymidylate synthase (TYMS) plays a key role for 5-fluorouracil metabolism and has been associated with some side effects caused by capecitabine. The aim of our study is to identify the possible genetic predictors of capecitabine-induced HFS (CAP-HFS) in Chinese colorectal cancer patients.Materials and Methods Whole exons of TYMS were sequenced for 288 extreme phenotype HFS patients, including 144 severe or early-onset (first 2 cycles) moderate HFS extreme cases and 144 extreme controls with no reported HFS. The associations between polymorphisms and CAP-HFS were analyzed using logistic regression under an additive model.Results We identified a novel risk mutation (c.1A>G, chr18:657743), was associated with severe HFS in an extreme case who was affected during the first cycle of treatment. Moreover, we identified three new variants, rs3786362, rs699517, rs2790, and two previously reported variants, 5’VNTR 2R/3R and 3′-untranslated region 6-bp ins-del, which were significantly associated with CAP-HFS (p < 0.05). In silico analysis revealed that the effect of these polymorphisms in the TYMS region on the development of HFS might not be restricted solely to the regulation of TYMS expression, but also the TYMS catalytic activity through the indirect effect on ENOSF1 expression.Conclusion This study identified new polymorphisms in TYMS gene significantly associated with CAP-HFS, which may serve as useful genetic predictors for CAP-HFS and help to elucidate the underlying mechanism of HFS.

Causal Relations between Exposome and Stroke: A Mendelian Randomization Study

Hong-Qi Li,Yi-Wei Feng,Yu-Xiang Yang,Xin-Yi Leng,Prof Can Zhang,Shi-Dong Chen,Kevin Kuo,Shu-Yi Huang,Xue-Qing Zhang,Yi Dong,Xiang Han,Xin Cheng,Mei Cui,Lan Tan,Qiang Dong,Jin-Tai Yu 대한뇌졸중학회 2022 Journal of stroke Vol.24 No.2

Background and Purpose To explore the causal relationships of elements of the exposome with ischemic stroke and its subtypes at the omics level and to provide evidence for stroke prevention. Methods We conducted a Mendelian randomization study between exposure and any ischemic stroke (AIS) and its subtypes (large-artery atherosclerotic disease [LAD], cardioembolic stroke [CE], and small vessel disease [SVD]). The exposure dataset was the UK Biobank involving 361,194 subjects, and the outcome dataset was the MEGASTROKE consortium including 52,000 participants. Results We found that higher blood pressure (BP) (systolic BP: odds ratio [OR], 1.02; 95% confidence interval [CI], 1.01 to 1.04; diastolic BP: OR, 1.03; 95% CI, 1.01 to 1.05; pulse pressure: OR, 1.03; 95% CI, 1.00 to 1.06), atrial fibrillation (OR, 1.18; 95% CI, 1.13 to 1.25), and diabetes (OR, 1.13; 95% CI, 1.07 to 1.18) were significantly associated with ischemic stroke. Importantly, higher education (OR, 0.69; 95% CI, 0.60 to 0.79) decreased the risk of ischemic stroke. Higher systolic BP (OR, 1.06; 95% CI, 1.02 to 1.10), pulse pressure (OR, 1.08; 95% CI, 1.02 to 1.14), diabetes (OR, 1.28; 95% CI, 1.13 to 1.45), and coronary artery disease (OR, 1.58; 95% CI, 1.25 to 2.00) could cause LAD. Atrial fibrillation could cause CE (OR, 1.90; 95% CI, 1.71 to 2.11). For SVD, higher systolic BP (OR, 1.04; 95% CI, 1.00 to 1.07), diastolic BP (OR, 1.06; 95% CI, 1.01 to 1.12), and diabetes (OR, 1.22; 95% CI, 1.10 to 1.36) were causal factors. Conclusions The study revealed elements of the exposome causally linked to ischemic stroke and its subtypes, including conventional causal risk factors and novel protective factors such as higher education.

Epidemiological Characteristics of Norovirus Outbreaks in Shenyang from 2017 to 2021

Qi Ying,Dong Xinxin,Cheng Xiaowei,Xu Han,Wang Jin,Wang Bing,Chen Ye,Sun Baijun,Zhang Linlin,Yao Yan 한국미생물학회 2023 The journal of microbiology Vol.61 No.4

Norovirus is one of the leading causes of acute gastroenteritis outbreaks worldwide. This study aimed to identify the epidemiological characteristics of norovirus outbreaks and to provide evidence for public health entities. Specimens and epidemiological survey data were collected to determine if there were differences in the attack rate of norovirus in terms of the year, season, transmission route, exposure setting, and region and to determine whether there were relationships between the reporting interval, the number of illnesses in a single outbreak and the duration of the outbreak. Norovirus outbreaks were reported throughout the year, with seasonal characteristics (i.e., high rates in spring and winter). Among all regions in Shenyang with the exception of Huanggu and Liaozhong, norovirus outbreaks had been reported, and the primary genotype was GII.2[P16]. Vomiting was the most common symptom. The main places of occurrence were childcare institutions and schools. The person-to-person route was the main transmission route. The median duration of norovirus was 3 days (IQR [interquartile range]: 2–6 days), the median reporting interval was 2 days (IQR: 1–4 days), the median number of illnesses in a single outbreak was 16 (IQR: 10–25); there was a positive correlation between these parameters. Norovirus surveillance and genotyping studies still need to be further strengthened to increase knowledge regarding the pathogens and their variant characteristics, to better characterize the patterns of norovirus outbreaks and to provide information for outbreak prevention. Norovirus outbreaks should be detected, reported and handled early. Public health entities and the government should develop corresponding measures for different seasons, transmission routes, exposure settings, and regions.

Novel efficient and environmentally friendly recovering of high performance nano-LiMnPO4/C cathode powders from spent LiMn2O4 batteries

Qi Meng,Jianguo Duan,Ying-Jie Zhang,Peng Dong 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.80 No.-

In this study, a novel efficient and environmentally friendly recycling process is developed to recoverspent LiMn2O4 powder as raw materials for high-performance LiMnPO4/C nanocomposites. The processcomprises a mechanochemical liquid-phase activation of the precursor mixture followed by a single-stepsolid-state heat treatment. The results indicate that the recovered LiMnPO4/C nano-composite has apromising electrochemical performance with discharge capacities of 148.5, 136.1, and 116.5 mA h g 1 at0.05, 0.2, and 1C rate, respectively, in the voltage range of 2.5–4.5 V. A perfect cyclic performance is alsodisplayed with a capacity retention of 98% after 100 cycles at 1C rate.

Cardiac-specific overexpression of Ndufs1 ameliorates cardiac dysfunction after myocardial infarction by alleviating mitochondrial dysfunction and apoptosis

Qi Bingchao,Song Liqiang,Hu Lang,Guo Dong,Ren Gaotong,Peng Tingwei,Liu Mingchuan,Fang Yexian,Li Chunyu,Zhang Mingming,Li Yan 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Myocardial infarction (MI) is the leading cause of premature death among adults. Cardiomyocyte death and dysfunction of the remaining viable cardiomyocytes are the main pathological factors of heart failure after MI. Mitochondrial complexes are emerging as critical mediators for the regulation of cardiomyocyte function. However, the precise roles of mitochondrial complex subunits in heart failure after MI remain unclear. Here, we show that NADH:ubiquinone oxidoreductase core subunit S1 (Ndufs1) expression is decreased in the hearts of heart failure patients and mice with myocardial infarction. Furthermore, we found that cardiac-specific Ndufs1 overexpression alleviates cardiac dysfunction and myocardial fibrosis in the healing phase of MI. Our results demonstrated that Ndufs1 overexpression alleviates MI/hypoxia-induced ROS production and ROS-related apoptosis. Moreover, upregulation of Ndufs1 expression improved the reduced activity of complex I and impaired mitochondrial respiratory function caused by MI/hypoxia. Given that mitochondrial function and cardiomyocyte apoptosis are closely related to heart failure after MI, the results of this study suggest that targeting Ndufs1 may be a potential therapeutic strategy to improve cardiac function in patients with heart failure.

Intercomparison of ADC-Lite Images on UAV and TM Simulation Data for Soybean Leaf Area Index Retrieval

Qi Zhang,Zhongbin Su,Guijun Yang,Minghui Wang,Weizheng Shen,Xiaowei Teng,Jinhui Dong 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.7

Currently, TM images has a very high practical value and widely used in all aspects of agricultural. Unmanned Aerial Vehicles (UAV) remote sensing platform mounted ADC-Lite multi-spectral sensor has consistent channels response functions with TM sensor in TM2, TM3 and TM4, demonstrated to compete with TM sensor, due to low operational cost, high operational flexibility, high spatial resolution of imagery (0.018m with flight altitude 50m) and heterogeneity both at time and spatial-scale. In order to make sure whether it has widely used as TM sensor, moreover, the aim of this work is to assess ADC-Lite performance such as its adaptability and practicability. In this paper, ADC-Lite multi-spectral data, ground truth ASD hyperspectral and Leaf area index (LAI) data were acquired in soybean planting area, Jiaxiang County, Shandong Province on September18th, 2015. Since the ADC-Lite has different spatial scales with TM, this paper used TM simulation data transformed by ground truth ASD data, constructed LAI inversion model by empirical model based on two sensors and ground measured data, using 5 vegetation indices: ratio vegetation index (RVI), normalized difference vegetation index (NDVI), soil adjust vegetation index (SAVI), difference vegetation index (DVI) and triangular vegetation index (TVI). Determination coefficient R2, root mean square error (RMSE) and the estimation accuracy (EA) 3 indicators were acquired to assess the model. This work suggests that the established model of ADC-Lite sensor with TM simulation sensor has high consistency in accuracy. NDVI linear regression model derived from both of them presented a strong correlation with ground-measured LAI. It’s preliminarily shown that ADC-Lite images assess soybean LAI is feasible. This is anticipated to have tremendous implications that ADC-Lite can be made supplement for existing satellites, aerial and ground sensing, provide important information for Crop condition monitoring and critical data to support crop maturity, nutrition monitoring and fertilization management.

Effect of humidity on mechanical properties of dissolving microneedles for transdermal drug delivery

Qi Lei Wang,Jia Wei Ren,Bo Zhi Chen,Xuan Jin,Can Yang Zhang,Xin Dong Guo 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.59 No.-

Dissolving microneedles (DMNs) prepared from soluble polymers has been being widely investigated as a safe and effective transdermal delivery system for drug and vaccine. Limited by the materials, the air humidity would probably affect the mechanical property of DMNs, which is essential for the effective needle insertions and drug delivery. In this study, four kinds DMNs prepared by polyvinyl alcohol, sodium hyaluronate (HA), chitosan and gelatin are studied on the mechanical strength and insertion ability under varying humidity conditions. Based on these investigations, we hope to provide the effective time of DMNs to ensure a successful insertion and drug delivery.

A novel process for leaching of metals from LiNi1/3Co1/3Mn1/3O2 material of spent lithium ion batteries: Process optimization and kinetics aspects

Qi Meng,Ying-Jie Zhang,Peng Dong,Feng Liang 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.61 No.-

A novel process of electrochemical cathode reduction has been developed for leaching of metals from spent LiNi1/3Co1/3Mn1/3O2 material. The leaching efficiencies of Li, Ni, Co and Mn reached to 100, 99.87, 99.58 and 99.82%, respectively with 1.5 mol/L malic acid and working voltage of 8 V at 300 r/min and 60 °C for 30 min. Kinetics analysis indicates that leaching process is controlled by surface chemical reaction. The Ea for Li, Ni, Co and Mn was 41.71, 42.83, 44.38 and 43.17 kJ/mol, respectively. Based on EIS analysis, leaching process is mainly controlled by charge transfer resistance of the surface chemical reaction.

The estimations of planing hull running attitude and resistance by using CFD and Goal Driven Optimization

ZHANG, Qi,KIM, Dong-Joon The Korean Society of Fisheries and Ocean Technolo 2015 수산해양기술연구 Vol.51 No.3

As a "kind of" mature ship form, planing hull has been widely used in military and civilian areas. Therefore, a reasonable design for planing hull becomes more and more important. For planing hull, resistance and trim are always the most important problems we are concerned with. It affects the planing hull's economic efficiency and maneuverability very seriously. Instead of the expensive towing tank experiments, the development of computer comprehensive ability allows us to previously apply computational fluid dynamics(CFD)to the ship design. In this paper, the CFD method and Goal Driven Optimization (GDO) were used in the estimations of planing hull resistance and running attitude to provide a possible method for performance computation of planing hull.

Pretreatments of Broussonetia papyrifera: in vitro assessment on gas and methane production, fermentation characteristic, and methanogenic archaea profile

Dong Lifeng,Gao Yanhua,Jing Xuelan,Guo Huiping,Zhang Hongsen,Lai Qi,Diao Qiyu 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.9

Objective: The present study was conducted to examine the gas production, fermentation characteristics, nutrient degradation, and methanogenic community composition of a rumen fluid culture with Broussonetia papyrifera (B. papyrifera) subjected to ensiling or steam explosion (SE) pretreatment. Methods: Fresh B. papyrifera was collected and pretreated by ensiling or SE, which was then fermented with ruminal fluids as ensiled B. papyrifera group, steam-exploded B. papyrifera group, and untreated B. papyrifera group. The gas and methane production, fermentation characteristics, nutrient degradation, and methanogenic community were determined during the fermentation. Results: Cumulative methane production was significantly improved with SE pretreatment compared with ensiled or untreated biomass accompanied with more volatile fatty acids production. After 72 h incubation, SE and ensiling pretreatments decreased the acid detergent fiber contents by 39.4% and 22.9%, and neutral detergent fiber contents by 10.6% and 47.2%, respectively. Changes of methanogenic diversity and abundance of methanogenic archaea corresponded to the variations in fermentation pattern and methane production. Conclusion: Compared with ensiling pretreatment, SE can be a promising technique for the efficient utilization of B. papyrifera, which would contribute to sustainable livestock production systems. Objective: The present study was conducted to examine the gas production, fermentation characteristics, nutrient degradation, and methanogenic community composition of a rumen fluid culture with Broussonetia papyrifera (B. papyrifera) subjected to ensiling or steam explosion (SE) pretreatment.Methods: Fresh B. papyrifera was collected and pretreated by ensiling or SE, which was then fermented with ruminal fluids as ensiled B. papyrifera group, steam-exploded B. papyrifera group, and untreated B. papyrifera group. The gas and methane production, fermentation characteristics, nutrient degradation, and methanogenic community were determined during the fermentation.Results: Cumulative methane production was significantly improved with SE pretreatment compared with ensiled or untreated biomass accompanied with more volatile fatty acids production. After 72 h incubation, SE and ensiling pretreatments decreased the acid detergent fiber contents by 39.4% and 22.9%, and neutral detergent fiber contents by 10.6% and 47.2%, respectively. Changes of methanogenic diversity and abundance of methanogenic archaea corresponded to the variations in fermentation pattern and methane production.Conclusion: Compared with ensiling pretreatment, SE can be a promising technique for the efficient utilization of B. papyrifera, which would contribute to sustainable livestock production systems.