정상 체중인 성인 남성에서 지방과다와 심혈관질환의 위험요인간의 관련성

권우성,김준수,채진욱,이근미,정승필,문용 영남대학교 의과대학 2003 Yeungnam University Journal of Medicine Vol.20 No.1

정상체중을 가진 성인 남성에서 지방과다(adiposity)와 심혈관계 질환의 위험요인과의 관련성을 알아보고자 하였다. 2001년 1월 3일부터 12월 31일까지 영남대 학교의료원에서 종합검진을 받은 만 28∼69세 성인 남성 129명을 대상으로 연령, 신체계측, 혈압, 공복 혈당, 혈청 지질대사치와 체성분 검사를 실시하였다. 체질량지수(BMI)가 18.5∼23 kg/㎡(76명)인 정상체중군과 23∼25 kg/㎡(53명)인 과체중군으로 분류하고, 체비방률, 체지방량과 위험인자들(수축기혈압, 이완기혈압, 공복시 혈당, 중성지방, 저밀도지단백, 고밀도지단백)의 상관관계를 분석하고, 정상체중군을 지방과다에 따라 3군으로 나누고, 각 군과 과체중군에서 심혈과 질환의 위험인자를 평가하였다. 정상체중의 제1군을 기준으로 제2, 3군과 과체중군의 위험인자 존재에 대한 비차비(odds ratio)를 조사하였다. 정상체중군에서 지방과다 변수들(adiposity variables: 체지방량, 체지방률)과 수축기 및 이완기 혈압, 저밀도지단백, 중성지방에서 유의한 상관관계가 있었다. (P<0.01). 나이를 보정하고 난 뒤 제1군을 기준으로 제3군(체지방율: 3.7, 체지방양: 4.7)과 과체중군(체지방율: 6.6, 체지방양 11.5)에서 위험인자에 대해 더 높은 비차비를 가졌다. 지방이 많은 정상체중의 성인남성은 적은 지방을 가진 성인 남성보다 심혈관 질환에서 더 높은 유병 위험도를 가졌다. 지방 측정은 정상체중군에서 심혈관계 질환의 위험인자외에 추가적인 정보를 제공할 수 있었다. Background: Most of all studies about the relation between the health risk and obesity are based on the European and American data. The purpose of this study is to examine the relation between adiposity and factors for cardio vacular disease (CVD) in normal weight individuals. Materials and Methods: Normal weight subjects with a body mass index (BMI) between 18.5 and 23 kg/㎡(76 subjects) and overweight subjects with a BMI between 23 and 25 kg/㎡ (53 subjects) were retained for this study. Normal weight subjects were divided into three group of each adiposity variable, then three group and the overweight group were evaluated for the presence of CVD risk factors and analyze the correlation coefficients between adiposity variables and risk factors controlled for age in normal weight, overweight groups. Using logistic regression analysis, the odds ratio (OR) for the prevalence of risk factors for each group of adiposity variables and the overweight group was estimated relative to the first group in normal weight subjects. Results: Systolic BP, diastolic BP, LDL cholestrol, HDL cholesterol, triglycerides in normal weight subjects were significantly correlated with all adiposity variables (P<0.01). Third group (3.7 for %fat and 4.7 for fat mass) of adiposity variables in the normal weight group and the overweight group (6.6 for %fat and 11.5 for fat mass) tended to have higher ORs compared to first group for risk factor variables. Conclusion: Normal weight subjects with elevated adiposity had higher prevalence of risk factors than normal weights subjects with less adiposity. Measuring of adiposity added additional information of cardiovascular disease risk factors in normal weight subjects.

혈중 콜레스테롤과 음독자실시도

최승필,권태욱,황주일,김세경 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

Objective: Several studies suggest that a low cholesterol concentration is associated with a greater than normal risk of mortality from suicide. The authors sought to determine whether a low cholesterol level is associated with a history of serious suicide attempts among drug ingested ED patients. Method: serum cholesterol levels of attempted suicide of 89 patients, aged 20-72 years, were obtained from the ED admission biochemical profiles. Results: The seriousness of suicide attempt was associated with low serum cholesterol concentration in all age subgroups. Conclusions: Low cholesterol concentration should be futher investigated as a potential biological marker of suicide risk.

정신병적 증상이 동반된 기면병 1예

홍승철,송정민,정종현,권용실,한진희,이성필 大韓神經精神醫學會 2003 신경정신의학 Vol.42 No.5

Narcolepsy is a sleep disorder characterized by excessive daytime sleepiness, cataplexy, sleep paralysis, hypnagogic hallucinations and disrupted nocturnal sleep. We report a patient with a diagnosis of schizophrenia who was subsequently diagnosed as narcolepsy. A case of a 22-year-old woman with concomitant narcolepsy and psychotic symptoms was discussed from a perspective of the diagnosis and therapeutic challenges, particularly on pharmcological treatment dealing with issues raised by their coexistence.

폐경 후 여성에서 체성분과 골밀도와의 관계

채진욱,김일희,권우성,이근미,정승필,문용 영남대학교 의과대학 2003 Yeungnam University Journal of Medicine Vol.20 No.1

Background: Body weight is an important factor that influence the bone density in postmenopausal women except estrogen dificiency. However, different results are reported about the relationship between body composition and bone density in the postmenopausal women. We have studied the relationship between age, body weight, body mass index (BMI), waist hip ratio (WHR), muscle mass, fat mass, fat free mass and bone density. Materials and Methods: We have studied 127 persons of postmenopausal women who visited university medical center and examined the inbody 3.0 and Dual Energy X-ray Absorptiometry (DEXA) from Jan, 2001 to Jun, 2002. they didn't have any disease and didn't received homone therapy, osteoprosis therapy or other medication that influence the bone density. Results: The numbers of study subjects is total 127 persons. Mean age is 56.9±5.14, mean weight is 59.3±8.7 kg, mean BMI is 25.37±3.16 (kg/㎡), mean fat mass is 20.02±5.05 kg, mean muscle mass is 37.49±4.50 kg, mean fat free mass is 39.80±4.70, mean BMD is 0.828±0.148 (g/㎠). In the result of linear regression analysis, age, height, weight, muscle mass, fat free mass, fat mass, BMI are significant determinants of BMD. In stepwise multiple regression analysis, age is the most significant determinant of BMD and besides age, fat mass is the most significant determinant of BMD among body composition. Conclusion: In postmenopausal women, age, height, weight, BMI, muscle mass, fat free mass, fat mass are significant determinants of BMD and besides age, fat free mass is the most significant determinant of BMD among the body composition. So, diet and exercise that increase fat free mass will contribute to bone density increment.

The Effects of Photodynamic Therapy for Vancomycin-resistant Enterococci

( Pil Seung Kwon ) 대한임상검사과학회 2011 대한임상검사과학회지(KJCLS) Vol.43 No.3

The aim of this study was to evaluate the effects of the photosensitizer photogem with light-emitting diode (LED) on vancomycin-resistant enterococci (VRE). Two VRE strains isolated from the feces of patients. that was identificated Enterococcus faecium (vanA) and Enterococcus gallinarum (vanC1) using traditional biochemical tests and confirmed VRE genotyping from using polymerase chain reaction. In addition, three strains were used Enterococcus. faecalis CDC-286 (vanA), E. faecalis CDC-583 (vanB) and E. gallinarum CDC-42 (vanC1). To examine the antimicrobial effect of photogem mediated photodynamic therapy (PDT) against, CFU quantification and Disk diffusion antimicrobial susceptibility test were evaluated. The effects of Photodynamic therapy was not associated with genotype. Photogem mediated PDT perfectly inhibited the colony formation of E. faecalis CDC-286. The number of viable bacteria decreased greatly after PDT application with photogem 50 μg/mL and energy density of 15 J/ ㎠. The diameter of inhibition zone was increased to after PDT more than before PDT. The case of vancomycin disc on E. faecalis CDC-583 and E. galinanum-Patient were changed from resistant to intermediate resistant, from intermediate resistant to susceptable. These results demonstrate that lethal photosensitization of VRE can be achieved using photogem plus 630 nm LED irradiation.

The Effects of Photodynamic Therapy for Vancomycin-resistant Enterococci

Kwon, Pil Seung 대한임상검사과학회 2011 대한임상검사과학회지(KJCLS) Vol.43 No.3

The aim of this study was to evaluate the effects of the photosensitizer photogem with light-emitting diode (LED) on vancomycin-resistant enterococci (VRE). Two VRE strains isolated from the feces of patients. that was identificated Enterococcus faecium (vanA) and Enterococcus gallinarum (vanC1) using traditional biochemical tests and confirmed VRE genotyping from using polymerase chain reaction. In addition, three strains were used Enterococcus. faecalis CDC-286 (vanA), E. faecalis CDC-583 (vanB) and E. gallinarum CDC-42 (vanC1). To examine the antimicrobial effect of photogem mediated photodynamic therapy (PDT) against, CFU quantification and Disk diffusion antimicrobial susceptibility test were evaluated. The effects of Photodynamic therapy was not associated with genotype. Photogem mediated PDT perfectly inhibited the colony formation of E. faecalis CDC-286. The number of viable bacteria decreased greatly after PDT application with photogem $50{\mu}g/mL$ and energy density of $15J/cm^2$. The diameter of inhibition zone was increased to after PDT more than before PDT. The case of vancomycin disc on E. faecalis CDC-583 and E. galinanum-Patient were changed from resistant to intermediate resistant, from intermediate resistant to susceptable. These results demonstrate that lethal photosensitization of VRE can be achieved using photogem plus 630 nm LED irradiation.

Antimicrobial Effects of Photodynamic Therapy Using Blue Light Emitting Diode with Photofrin and Radachlorine against Propionibacterium acnes

Kwon, Pil-Seung 대한임상검사과학회 2015 대한임상검사과학회지(KJCLS) Vol.47 No.1

Photodynamic therapy (PDT) apply photosensitizers and light. The purpose of this study was to evaluate the in vitro efficacy of PDT using blue LED (light emitting diode) with photofrin and radachlorin for Propionibacterium acnes. The colony forming units method was used to assess the antibacterial activity. Suspension (1 mL) containing P. acnes at $1{\times}10^5CFU/mL$ were prepared and then 2 fold serial diluted to $12.5{\mu}g/mL$ from $50{\mu}g/mL$ concentration of photofrin and radachlorin. After 60 minutes incubation, light was irradiated for 10 to 30 minutes using the following light source of wavelength 460 nm, each energy density 36, 72 and $108J/cm^2$. Bacterial growth was evaluated after 72 hours incubation in a Phenylethanol Blood Agar (PEBA) culture. In addition, flow cytometric analysis were performed to measure the live cell after PDT. Also transmission electron microscopy (TEM) was employed to evaluate the effect of pathogens by PDT. The PDT Group was perfectly killed to all kind of photosensitizers dose of $12.5{\mu}g/mL$ with irradiation of 10 minutes. Also other Groups were killed to all kind of photosensitizers dose of $6.25{\mu}g/mL$ with irradiation time of 20 and 30 minutes. The flow cytometry showed a lower number of viable bacteria in the PDT group compared to the control group. The images of the TEM results were showed in cytoplasmic membrane damage and partially deformed to cell morphologies. These results suggest that radachlorin and photofrin combine blue LED PDT can be effectively treated when was proved treatment for acnes therapy.

Photodynamic Therapy for Methicillin-resistant Staphylococcus aureus with High-level Mupirocin Resistance using 630 ㎚ Light-emitting Diode

Pil Seung Kwon(권필승),Jin-Kyung Kim 대한의생명과학회 2010 Biomedical Science Letters Vol.16 No.4

This study aims to evaluate the effect of Photodynamic Therapy (PDT) against methicillin-resistant Staphylococcus aureus with high-level mupirocin resistance (Hi-Mup MRSA). To examine the antimicrobial effect of photogemmediated PDT against Hi-Mup MRSA, CFU quantifications, bacteria cell viability tests, and disk diffusion antimicrobial susceptibility tests were evaluated. In addition, one of PDT mechanisms was investigated by accumulating photogem (10 ㎍/㎖) in Hi-Mup MRSA. Photogem-mediated PDT properly inhibited the colony formation of Hi-Mup MRSA. Viable bacteria decreased greatly after a PDT application with photogem 10 ㎍/㎖ at energy density 15 J/㎠. The diameter of the inhibition zone around susceptible disks increased after PDT. In addition, we confirmed the accumulation of photogem in bacteria through fluorescent images. These results demonstrated that excellent photosensitization of Hi-Mup MRSA can be achieved using photogem with 630 nm LED irradiation. Thus, PDT may make survival Hi-Mup MRSA inactive.

Photodynamic Therapy for Methicillin-resistant Staphylococcus aureus Using Various Photosensitizer

Pil Seung Kwon,Yoon kyung Jo(조윤경) 대한의생명과학회 2009 Biomedical Science Letters Vol.15 No.3

The aim of this study was to evaluate the photodynamic effect of various photosensitizing agents against methicillinresistant Staphylococcus aureus (MRSA). MRSA was exposed to light from a 632 ㎚ diode laser (15 J/㎠) in the presence of various photosensitizer, such as photofrin, photogem, radachlorine and ALA. In vivo study was performed using ICR mice. Twenty eight mice had a standard wound (100 ㎟) created on the dorsum, and MRSA was inoculated into the wound region. The four groups were classified as follows: (1) the untreated control group (bacteria alone), (2) the bacteria plus light group (15 J/㎠), (3) the bacteria plus photofrin group (kept in the dark), and (4) the photodynamic therapy (PDT) group (bacteria, photofrin, and light). After photofrin (dose 1 mg/kg) injection, the experiemental group was irradiated with 632 ㎚ diode laser (15 J/㎠) for 30 minutes after In vitro results of PDT showed the complete killing of MRSA at the photofrin, radachlorine, and photogem However, ALA-PDT was ineffective on MRSA viability. In vivo results showed that photofrin has therapeutic effect on the wound infection. These results demonstrate that selective lethal photosensitization of MRSA can be achieved using phofrin, photogem and radachlorin. Thus, PDT can inactivate MRSA survival.

Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry의 활용

KWON Pil Seung 대한임상검사과학회 2023 대한임상검사과학회지(KJCLS) Vol.55 No.4

The timeliness and accuracy of test results are crucial factors for clinicians to decide and promptly administer effective and targeted antimicrobial therapy, especially in life-threatening infections or when vital organs and functions, such as sight, are at risk. Further research is needed to refine and optimize matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based assays to obtain accurate and reliable results in the shortest time possible. MALDI-TOF MS-based bacterial identification focuses primarily on techniques for isolating and purifying pathogens from clinical samples, the expansion of spectral libraries, and the upgrading of software. As technology advances, many MALDI-based microbial identification databases and systems have been licensed and put into clinical use. Nevertheless, it is still necessary to develop MALDI-TOF MS-based antimicrobial-resistance analysis for comprehensive clinical microbiology characterization. The important applications of MALDI-TOF MS in clinical research include specific application categories, common analytes, main methods, limitations, and solutions. In order to utilize clinical microbiology laboratories, it is essential to secure expertise through education and training of clinical laboratory scientists, and database construction and experience must be maximized. In the future, MALDI-TOF mass spectrometry is expected to be applied in various fields through the use of more powerful databases.