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Prognostic Factors and Decision Tree for Long-Term Survival in Metastatic Uveal Melanoma
Daniel Lorenzo,María Ochoa,Josep Maria Piulats,Cristina Gutiérrez,Luis Arias,Jaume Català,María Grau,Judith Peñafiel,Estefanía Cobos,Pere Garcia-Bru,Marcos Javier Rubio,Noel Padrón-Pérez,Bruno Dias,Jo 대한암학회 2018 Cancer Research and Treatment Vol.50 No.4
Purpose The purpose of this study was to demonstrate the existence of a bimodal survival pattern in metastatic uveal melanoma. Secondary aims were to identify the characteristics and prognostic factors associated with long-term survival and to develop a clinical decision tree. Materials and Methods The medical records of 99 metastatic uveal melanoma patients were retrospectively reviewed. Patients were classified as either short ( 12 months) or long-term survivors (> 12 months) based on a graphical interpretation of the survival curve after diagnosis of the first metastatic lesion. Ophthalmic and oncological characteristics were assessed in both groups. Results Of the 99 patients, 62 (62.6%) were classified as short-term survivors, and 37 (37.4%) as long-term survivors. The multivariate analysis identified the following predictors of long-term survival: age 65 years (p=0.012) and unaltered serum lactate dehydrogenase levels (p=0.018); additionally, the size (smaller vs. larger) of the largest liver metastasis showed a trend towards significance (p=0.063). Based on the variables significantly associated with long-term survival, we developed a decision tree to facilitate clinical decision-making. Conclusion The findings of this study demonstrate the existence of a bimodal survival pattern in patients with metastatic uveal melanoma. The presence of certain clinical characteristics at diagnosis of distant disease is associated with long-term survival. A decision tree was developed to facilitate clinical decision-making and to counsel patients about the expected course of disease.
Edwin David Morales-Álvarez,Claudia Marcela Rivera-Hoyos,Angélica María Baena-Moncada,Patricia Landázuri,Raúl A. Poutou-Piñales,Homero Sáenz-Suárez,Luis A. Barrera,Olga Y. Echeverri-Peña 한국미생물학회 2013 The journal of microbiology Vol.51 No.2
The sulfatase family involves a group of enzymes with a large degree of similarity. Until now, sixteen human sulfatases have been identified, most of them found in lysosomes. Human deficiency of sulfatases generates various genetic disorders characterized by abnormal accumulation of sulfated intermediate compounds. Mucopolysaccharidosis type II is characterized by the deficiency of iduronate 2-sulfate sulfatase (IDS), causing the lysosomal accumulation of heparan and dermatan sulfates. Currently, there are several cases of genetic diseases treated with enzyme replacement therapy, which have generated a great interest in the development of systems for recombinant protein expression. In this work we expressed the human recombinant IDS-Like enzyme (hrIDS-Like) in Escherichia coli DH5α. The enzyme concentration revealed by ELISA varied from 78.13 to 94.35 ng/ml and the specific activity varied from 34.20 to 25.97 nmol/h/mg. Western blotting done after affinity chromatography purification showed a single band of approximately 40 kDa, which was recognized by an IgY polyclonal antibody that was developed against the specific peptide of the native protein. Our 100 ml-shake-flask assays allowed us to improve the enzyme activity seven fold,compared to the E. coli JM109/pUC13-hrIDS-Like system. Additionally, the results obtained in the present study were equal to those obtained with the Pichia pastoris GS1115/pPIC-9-hrIDS-Like system (3 L bioreactor scale). The system used in this work (E. coli DH5α/pGEX-3X-hrIDS-Like)emerges as a strategy for improving protein expression and purification, aimed at recombinant protein chemical characterization,future laboratory assays for enzyme replacement therapy, and as new evidence of active putative sulfatase production in E. coli.
Agrobacterium tumefaciens-mediated genetic transformation of Digitalis purpurea L.
Naivy Pe´rez-Alonso,Elio Jime´nez,Borys Chong-Pe´rez,Alina Capote,Anabel Pe´rez,Yovanny Izquierdo,Geert Angenon 한국식물생명공학회 2014 Plant biotechnology reports Vol.8 No.5
Genetic transformation is a tool of specialinterest for developing new biotechnological strategies forthe production of bio-active compounds such as cardenolides,which are exclusively obtained from plants. To date,Digitalis plants are the main economically viable source ofcardenolides for the pharmaceutical industry. This studydescribes the development of efficient plant regenerationand Agrobacterium-mediated genetic transformation protocolsfor Digitalis purpurea L. First, a plant regenerationprocedure starting from leaf segments of in vitro-cultivatedplants was established and the minimal inhibitory concentrationof G-418 (geneticin) for callus induction wasdetermined. Both leaf segments and callus tissue weresensitive to G-418 70 mg l-1. Afterwards, two Agrobacteriumstrains were used to test their T-DNA transferability on D. purpurea leaf tissues, EHA105 andC58C1RifR (pMP90), both harboring the binary vectorpTJK136. Strain C58C1RifR (pMP90) yielded a highernumber of transformed plants than EHA105. Successfultransformation was confirmed by histochemical b-glucuronidase(GUS) assays of the putative transgenic tissuesand PCR analyses using b-glucuronidase (uidA)- andneomycin phosphotransferase II (nptII)-specific primers. Southern blot hybridization confirmed the stable integrationof the nptII gene in the transgenic plants. In total, 518independent transgenic lines were regenerated with anaverage of 6.91 transgenic lines per initial leaf segmentinfected with A. tumefaciens strain C58C1RifR (pMP90). To date, only a few studies have been published on thegenetic transformation of Digitalis species. The protocolsfor plant regeneration and genetic transformation describedin this paper will contribute to functional studies for abetter understanding of cardenolide biosynthetic pathwaysand the metabolic engineering of cardenolides to develophigh-yielding improved genotypes.
REAL HYPERSURFACES OF QUATERNIONIC PROJECTIVE SPACE SATISFYING ▽_(U_(i))R=O
Pe´rez, Juan de Dios,Suh, Young Jin 경북대학교 위상수학 기하학연구센터 1995 硏究論文集 Vol.5 No.-
we classify real hypersurfaces of quaternionic projective space satisfying ▽_(Ui) R=0, i=1, 2, 3.
Macrina Pe´rez-Lo´pez,Rodolfo Garcı´a-Contreras,Marcos Soto-Herna´ndez,Jose´ Salud Rodrı´guez-Zavala,Mariano Martı´nez-Va´zquez,Francisco Javier Prado-Galbarro,Israel Castillo-Jua´rez 한국식품영양과학회 2018 Journal of medicinal food Vol.21 No.4
Seed oils from oleaginous plants are rich in fatty acids (FAs) that play important roles in the health of the consumers. Recent studies indicate that FA also can play an important role in communication and regulation of virulence in bacteria. Nevertheless, evidence demonstrating protection against bacterial infections mediated by their quorum sensing inhibition (QSI) activity is scarce. In this study, sunflower, chia, and amaranth oils, were assayed for their QSI capacity by inhibiting violacein production and alkaline exoprotease activity of Chromobacterium violaceum. In vitro assays revealed that the oils exhibited QSI activities, whereas in vivo they delayed death of mice inoculated intraperitoneally with the bacterium. Gas chromatography coupled with mass spectrometry analysis of the oils indicated the presence of saturated FA (SAFA) and unsaturated FA as main components. Through a structure–activity relationship study of free FAs, bactericidal effect was identified mainly for polyunsaturated FAs, whereas QSI activity was restricted to SAFA of chains 12–18 carbon atoms in length. These data correlate with a possible interaction suggested by molecular docking analysis of lauric, myristic, and stearic acids with the CviR protein. Our study highlights the antiquorum sensing potential of SAFA, which may be future antivirulence therapeutic agents for the treatment of bacterial infections.
Blanco, Lucrecio Pe′rez 서울大學校 스페인中南美硏究所 1996 이베로아메리카硏究 Vol.7 No.-
Fue en la primavera de 1988, tiempo en que yo ayudaba al profesor Kim a publicar en español su primer libro de poemas de So Jong-Ju, y dado a la luz bajo el amparo de la Universidad Complutense en ese mismo año de 1988 con el titulo de Junto al crisantemo, cuando me senti acosado como un niño caprichoso por el poema creano: '¿Conoce usted un pais/ donde viven personas ma´s tranquilas y solitarias/ que un suen∼o tranquilo/ o la meditacio´n de un bonzo/ en el sono de una montaña/ o que la medianoche?"
J.F. Ramı´rez-Pe´ rez,R. Herna´ndez-Altamirano,J.M. Martı´nez-Magada´n,R. Cartas-Rosado,E. Soto-Castruita,R. Cisneros-Devora,L.A. Alca´ zar-Vara,R. Oviedo-Roa,V.Y. Mena-Cervantes,L.S. Zamudio-Rivera 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.45 No.-
A new class of branched geminal zwitterionic liquids (BGZLs) betaine base with a long alkyl chains witheither 8 (BGZL-ZW8) or 12 (BGZL-ZW12) carbons was synthesized and characterized by nuclearmagnetic resonance (NMR) and Fourier transform infrared (FTIR) spectroscopic techniques. The BGZLsmolecules presents functionality as viscosity reducer and wettability modifier with application inenhanced oil recovery (EOR) processes with good oil recovery factors (ORF). The above is demonstratedby performing spontaneous imbibition tests on embedded limestone cores with light and heavy crudeoils in Amott cells at high temperature (90 8C) and different concentrations of BGZLs in presence ofconnate water with high salinity and hardness (36,275 ppm as NaCl and 6700 ppm hardness as CaCO3). The rheological behavior of BGZLs was also determined as viscosity reducers in heavy crude oil atconcentrations of 2000, 1000 and 500 ppm at temperatures of 25 and 40 8C, in the range of 36.11% to25 8C and 43.31% to 40 8C. To explain the mechanism through BGZLs act as viscosity reducers, werealized theoretical studies using the density functional theory (DFT), also as wettability modifierstaking BGZL-ZW8 and BGZL-ZW12 behavior, observed in the experimental part. The interaction energiesshow that BGZLs can disaggregate asphaltene (Asph) dimmer (Asph:Asph) by the formation ofsupramolecular sandwich complex (Asph:BGZL:Asph) among BGZLs and Asph:Asph, modifying theproperties of crude oil such as viscosity, is possible to support the fact of the BGZLs can desorbs Asphmolecules of the rock surface, modifying the calcite surface (Cs) wettability of oil-wet to water-wet. Theresults of this research show that it is feasible the use of zwitterionic liquids as viscosity and wettabilitymodifier in EOR process.
Susana Herna´ndez-Pe´rez,Rosa Marı´a Oliart-Ros,Leticia Casas-Godoy,Georgina Sandoval,Veronica Guarner Lans,Vicente Castrejo´n-Te´llez,Lucı´a Quevedo-Corona,Lucı´a Quevedo-Corona,Carolina Pen˜a-Montes 한국식품영양과학회 2022 Journal of medicinal food Vol.25 No.8
Metabolic syndrome (MS) is a group of abnormalities in which obesity, insulin resistance (IR), oxidative stress, and dyslipidemia stand out. This pathology predisposes to the development of cardiovascular diseases and diabetes. The ingestion of linear fructooligosaccharides (FOS) such as inulin reduces conditions such as hyperinsulinemia, increased body fat, and triglyceridemia. When FOS are esterified with fatty acids, they present emulsifying and surfactant properties; however, there are no reports of their function at the biological level. The purpose of this investigation was to evaluate the effect of Agave tequilana Weber's FOS (AtW-FOS) and FOS esterified with lauric acid (FOS-LA) in MS markers in a rat model induced by a HFHC diet. Supplementation with AtW-FOS and FOS-LA decreased IR, improved glucose tolerance, reduced liver weight (19%), plasma triglycerides (24%), and blood pressure (16%) when compared with the untreated MS group. In conclusion, the ingestion of AtW-FOS and FOS-LA has beneficial effects in the prevention of MS alterations, showing a high potential for their application in functional foods.