Highly sensitive and selective electrochemical sensor for detection of vitamin B12 using an Au/PPy/FMNPs@TD-modified electrode

Parvin, Mohammad Hadi,Azizi, Elmira,Arjomandi, Jalal,Lee, Jin Yong Elsevier 2018 Sensors and actuators. B Chemical Vol.261 No.-

<P><B>Abstract</B></P> <P>A new electrochemical sensor for sensitive and selective detection of vitamin B12 (VB12) was constructed by the electropolymerization of pyrrole (Py) in the presence of ferromagnetic nanoparticle-incorporated triazine dendrimer (FMNPs@TD) on a gold electrode (Au). The gold/polypyrrole/ferromagnetic nanoparticles/triazine dendrimer (Au/PPy/FMNPs@TD) electrode showed electrocatalytic activity for the reduction of VB12 in Britton-Robinson buffers. The performance and interaction of the Au/PPy/FMNPs@TD with VB12 were investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), differential pulse voltammetric (DPV), UV–vis spectroelectrochemistry, and density functional theory calculations. The resulting sensor exhibited excellent performance for determination of VB12 with a wide linear range (2.50 nM–0.5 μM), highly reproducible response (RSD of 2.3%), low percentage of interferences, and long-term stability. The limit of detection (LOD) and limit of quantitation (LOQ) values for the determination of VB12 were 0.91 and 3.00 nM, respectively. The electron transfer rate constants (k<SUB>s</SUB>), charge transfer coefficient (α), surface concentrations of electroactive species (Г), and disproportionation equilibrium constant (K<SUB>D</SUB>) for Au/PPy/FMNPs@TD-modified electrodes were calculated. Finally, the constructed sensor was applied to the determination of VB12 in food samples.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A highly sensitive and selective sensor for detection of VB12 was fabricated. </LI> <LI> The sensor showed electrocatalytic activity for the reduction of VB12. </LI> <LI> The VB12 sensor exhibited long term stability and highly reproducible response. </LI> <LI> The LOD and LOQ values for the determination of VB12 were 0.91 and 3.00 nM. </LI> <LI> The sensor was applied to the determination of VB12 in food samples. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Longitudinal standards for growth velocity of infants from birth to 4 years born in West Azerbaijan Province of northwest Iran

Parvin Ghaemmaghami,Seyyed Mohammad Taghi Ayatollahi,Vahid Alinejad,Elham Haem 한국역학회 2015 Epidemiology and Health Vol.37 No.-

OBJECTIVES: Growth velocity is an important factor to monitor for appropriate child growth. This study presents the growth velocity of infants based on length, weight, and head circumference. METHODS: The subjects of this study were 308 neonates (160 boys and 148 girls) born in West Azerbaijan Province of northwestern Iran who were followed from birth for 4 years. The weights and lengths of the subjects were recorded at birth, 1, 2, 4, 6, and 9 months, and 1, 1.5, 2, 3, and 4 years of age, while the head circumferences were measured just up to 1.5 years of age. In this study, the Lambda-Mu-Sigma (LMS) method using LMS Chartmaker Pro (Institute of Child Health, London, UK) was utilized to obtain growth velocity percentiles. RESULTS: After obtaining growth velocity charts for weight, length, and head circumference (5th, 50th, and 95th percentiles), the researchers could deduce that there was a sharp decrease in the velocity growth charts from birth to 2 years of age but these charts remained relatively stable up to 4 years for both sexes. Growth velocities for the length and weight of boys in the present sample are slightly but not significantly greater than those in girls through the first months of infancy and there was no significant difference between girls and boys up to 4 years. CONCLUSIONS: This paper provided the first local growth velocity standards of length, weight, and head circumference for infants by analyzing longitudinal measurements produced for West Azerbaijan Province, which should be updated periodically. It seems that there has been a significant difference between the growth velocity of infants in northwestern Iran and southern Iran within the past few years.

The Effect of Estrogen Replacement Therapy on Visceral Fat, Serum Glucose, Lipid Profiles and Apelin Level in Ovariectomized Rats

( Parvin Babaei ),( Adele Dastras ),( Bahram Soltani Tehrani ),( Shiva Pourali Roudbaneh ) 대한폐경학회 2017 대한폐경학회지 Vol.23 No.3

Objectives: Ovarian hormones have been shown to regulate body weight, intra-abdominal fat accumulation and plasma level of cytokines. The aim of this study was to investigate the effect of estrogen replacement therapy on visceral adipose tissue, plasma level of apelin, lipid profiles, and glucose in ovariectomized (OVX) rats. Methods: Thirty female Wistar rats were divided into OVX (n = 20) and sham (n = 10) groups. OVX rats were subdivided into estrogen replacement therapy (OVX+est; n = 10) receiving 17 β-estradiol valerates (30 μg/kg, s.c., 5 day/week, for eight weeks), and vehicle control group receiving sesame oil same as experiment group (OVX+ses oil; n = 10). After the treatments, all groups were sacrificed and blood samples were collected, visceral fats were taken from the abdominal cavity and weighed immediately. Apelin were measured using enzyme-linked immunosorbent assay kits. Lipid profiles and glucose were measured using the enzymatic colorimetric method. Data were analyzed with one-way analysis of variance and (P < 0.05) determined as the statistical significance level. Results: After eight weeks, body weight, body mass index (BMI), visceral fat, apelin and lipid profiles (P < 0.01) were increased significantly in OVX rats compared to sham group. Treatment with estrogen leads to significant reduction in body weight and BMI (P < 0.05), there was no significant change in serum apelin level in OVX+est rats compared to OVX+ses. Conclusions: These results suggest that estradiol replacement therapy successfully attenuated some of the metabolic syndrome components, and apelin does not probably stand as a mediator of these physiological functions. (J Menopausal Med 2017;23:182-189)

Biochemical characterization of digestive proteases and carbohydrases of the carob moth, Ectomyelois ceratoniae (Zeller) (Lepidoptera: Pyralidae)

Parvin Razavi Tabatabaei,,Vahid Hosseininaveh,Seyed Hossein Goldansaz,Khalil Talebi 한국응용곤충학회 2011 Journal of Asia-Pacific Entomology Vol.14 No.2

Digestive proteinases and carbohydrases of Ectomyelois ceratoniae (Zeller) larvae were investigated using appropriate substrates and inhibitors. Midgut pH in larvae was determined to be slightly alkaline. Midgut extracts showed optimum activity for proteolysis of hemoglobin at pH 9–12. Midgut proteinases also hydrolyzed the synthetic substrates of trypsin, chymotrypsin, and elastase at pH 8–11. Maximum digestive α-amylase activity was also observed at pH 8–11. However, optimum activity for α- and β-glucosidase occurred at pH 5–8. Alpha- and β-galactosidases optimum activities occurred at pH 5 and pH 6, respectively. Inhibitors of serine proteases were effective on midgut serine proteases (trypsin and chymotrypsin proteases). Zymogram analyses revealed at least five bands of total proteolytic activity in the larval midgut. Proteasespecific zymogram analyses revealed at least four, two, and one isozymes for trypsin-, chymotrypsin-, and elastase-like activities respectively. Two α-amylase isozymes were found in the midgut of fifth instar larvae and in the whole bodies of 1st through 5th instar larvae. Zymogram studies also revealed the presence of one and two bands of activity for β- and α-glucosidase, respectively. Recycling of α-amylase and proteases in the larval midgut was not complete. At least one isozyme of trypsin, chymotrypsin, elastase, and α-amylase were not recycled and were observed in the larval hindgut.

Dietary Sodium to Potassium Ratio and the Incidence of Chronic Kidney Disease in Adults

Parvin Mirmiran,Pantea Nazeri,Zahra Bahadoran,Sajjad Khalili-Moghadam,Fereidoun Azizi 한국식품영양과학회 2018 Preventive Nutrition and Food Science Vol.23 No.2

The aim of this study was to explore the association of dietary sodium to potassium (Na/K) ratio and the risk of chronic kidney disease (CKD) in general Iranian adults. In this prospective cohort study, 1,780 adults, free of baseline CKD with complete follow-up data, were selected from among participants of the Tehran Lipid and Glucose Study and followed for 6.3 years for development of CKD. Dietary sodium and potassium were assessed using a valid and reliable 168-item food frequency questionnaire. Estimated glomerular filtration rate (eGFR) was calculated using the Modification of Diet in Renal Disease Study equation, and CKD was defined as eGFR <60 mL/min/1.73 ㎡. Mean dietary intakes of sodium and potassium were 4,547±3,703 and 3,753±1,485 mg/d, respectively, and their ratio was 1.35±1.29. No significant association was found between dietary intakes of sodium and potassium and the risk of CKD after 6.3 y of followup, whereas in the case of dietary Na/K ratio, participants in the highest compared to lowest tertile (2.43 vs 1.61) had a significantly increased risk of CKD (odds ratio=1.52, 95% confidence interval=1.01∼2.30); an increasing trend in the risk of CKD across tertiles of dietary sodium to potassium ratio was also observed (P for trend=0.05). Present findings demonstrate that the dietary Na/K ratio is a stronger predictor of CKD than the dietary sodium or potassium per se. Decreased dietary Na/K ratio may be considered as an effective dietary approach to modify the risk of kidney dysfunction.

The interaction effects of aerobic exercise training and vitamin D supplementation on plasma lipid profiles and insulin resistance in ovariectomized rats

( Parvin Babaei ),( Arsalan Damirchi ),( Rastegar Hoseini ) 한국운동영양학회 2015 Physical Activity and Nutrition (Phys Act Nutr) Vol.19 No.3

[Purpose] The purpose ofthis study was to determine the interaction effects of aerobic exercise training and vitamin D supplementation on indices of obesity and plasma lipid profiles in ovariectomized (OVX) rats. [Methods] Forty female Wistar rats were divided into 5 groups: aerobic training (3 days/week for 8 weeks; AT; n = 8), aerobic training and vitamin D supplementation (OVX + AT + Vit D; n = 8), vitamin D supplementation (OVX + Vit D; n = 8), ovariectomized control (OVX + C, n = 8) and SHAM (n = 8). After blood sampling, visceral fat was taken from the abdominal cavity and weighed immediately. Data was statistically analyzed by One-way ANOVA and Repeated measure ANOVA tests with a 0.05 significance level. [Results] Body weight, visceral fat, BMI and food intake decreased significantly in OVX + AT + Vit D (P < 0.001); whereas these variables increased significantly in OVX + C (P < 0.001) and SHAM (P < 0.023) groups. At the end of two-months of follow-up, we observed significant differences in TC, TG, HDL-C, LDL-C, glucose, insulin, and HOMA-IR in all groups. [Conclusion] It seems that aerobic training with vitamin D, due to the involvement of muscle mass and exposure to dynamic pressure on the bones and muscles, increased energy expenditure, stimulated insulin exudation and glucose homeostasis, decreased insulin resistance and improved the lipid profile in ovariectomized rats.

Identification and characterization of spermidine synthase gene from Panax ginseng

Parvin, Shohana,Kim, Yeon-Ju,Pulla, Rama Krishna,Sathiyamoorthy, S.,Miah, Md. Giashuddin,Kim, Yu-Jin,Wasnik, Neha G.,Yang, Deok-Chun Springer-Verlag 2010 MOLECULAR BIOLOGY REPORTS Vol.37 No.2

Survivability Evaluation Model in Wireless Sensor Network using Software Rejuvenation

Parvin, Sazia,Thein, Thandar,Kim, Dong-Seong,Park, Jong-Sou Korea convergence Security Association 2008 융합보안 논문지 Vol.8 No.1

The previous works in sensor networks security have focused on the aspect of confidentiality, authentication and integrity based on cryptographic primitives. There has been no prior work to assess the survivability in systematic way. Accordingly, this paper presents a survivability model of wireless sensor networks using software rejuvenation for dual adaptive cluster head. The survivability model has state transition to reflect status of real wireless sensor networks. In this paper, we only focus on a survivability model which is capable of describing cluster head compromise in the networks and able to switch over the redundant cluster head in order to increase the survivability of that cluster. Second, this paper presents how to enhance the survivability of sensor networks using software rejuvenation methodology for dual cluster head in wireless sensor network. We model and analyze each cluster as a stochastic process based on Semi Markov Process (SMP) and Discrete Time Markov Chain (DTMC). The proof of example scenarios and numerical analysis shows the feasibility of our approach.

Dietary L-Arginine Intakes and the Risk of Metabolic Syndrome

Parvin Mirmiran,Sajjad Khalili Moghadam,Zahra Bahadoran,Asghar Ghasemi,Fereidoun Azizi 한국식품영양과학회 2017 Preventive Nutrition and Food Science Vol.22 No.4

This study was conducted to investigate whether regular dietary intake of L-arginine could affect the occurrence of metabolic syndrome (MetS). Eligible adult men and women (n=1,237), who participated in the Tehran Lipid and Glucose Study, were followed for a median of 6.3 years. Dietary intakes of L-arginine and serum nitrate and nitrite (NOx) concentration were assessed at baseline (2006∼2008), and demographics, anthropometrics, and biochemical variables were evaluated at baseline and follow-up examinations. The occurrence of MetS was assessed in relation to total L-arginine, intakes of L-arginine from animal and plant sources, with adjustment of potential confounding variables. Participants who had higher intake of L-arginine also had higher serum NOx at baseline (35.0 vs. 30.5 μmol/L, P<0.05). After 6 years of follow-up, higher intakes of L-arginine from animal sources were accompanied with increased risk of MetS [odd ratios (OR)=1.49, 95% confidence interval (95% CI)=1.02∼2.18]. Compared to the lowest, the highest intakes of L-arginine from plant sources were related to significantly reduced risk of MetS (OR=0.58, 95% CI=0.32∼0.99). In conclusion, our findings suggest a potentially protective effect of plant derived L-arginine intakes against development of MetS and its phenotypes; moreover, higher intakes of L-arginine from animal sources could be a dietary risk factor for development of metabolic disorders.

Lipid Accumulation Product Is Associated with Insulin Resistance, Lipid Peroxidation, and Systemic Inflammation in Type 2 Diabetic Patients

Parvin Mirmiran,Zahra Bahadoran,Fereidoun Azizi 대한내분비학회 2014 Endocrinology and metabolism Vol.29 No.4

Background: Lipid accumulation product (LAP) is a novel biomarker of central lipid accumulation related to risk of diabetes and cardiovascular disease. In this study, we assessed the association of LAP with glucose homeostasis, lipid and lipid peroxidation, and subclinical systemic inflammation in diabetic patients. Methods: Thirty-nine male and 47 female type 2 diabetic patients were assessed for anthropometrics and biochemical measurements. LAP was calculated as [waist circumference (cm)–65]×[triglycerides (mmol/L)] in men, and [waist circumference (cm)–58]×[triglycerides (mmol/L)] in women. Associations of LAP with fasting glucose, insulin, insulin resistance index, lipid and lipoprotein levels, malondialdehyde, and high-sensitive C-reactive protein (hs-CRP) were assessed. Results: Mean age and LAP index were 53.6±9.6 and 51.9±31.2 years, respectively. After adjustments for age, sex and body mass index status, a significant positive correlation was observed between LAP index and fasting glucose (r=0.39, P<0.001), and homeostasis model assessment of insulin resistance (r=0.31, P<0.05). After additional adjustment for fasting glucose levels, antidiabetic and antilipidemic drugs, the LAP index was also correlated to total cholesterol (r=0.45, P<0.001), high density lipoprotein cholesterol (HDL-C) levels (r=–0.29, P<0.05), triglycerides to HDL-C ratio (r=0.89, P<0.001), malondialdehyde (r=0.65, P<0.001), and hs-CRP levels (r=0.27, P<0.05). Conclusion: Higher central lipid accumulation in diabetic patients was related to higher insulin resistance, oxidative stress and systemic inflammation.