Muscle-specific color stability in fresh beef from grain-finished Bos indicus cattle

Ana Paula A. A. Salim,Surendranath P. Suman,Anna C.V.C.S. Canto,Bruno R.C. Costa-Lima,Fernanda M. Viana,Maria Lucia G. Monteiro,Teofilo J. P. Silva,Carlos A. Conte-Junior 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.7

Objective: To investigate the color and oxidative stabilities of longissimus lumborum (LL) and psoas major (PM) muscles from grain-finished Bos indicus cattle in Brazil. Methods: The LL and PM muscles were obtained 24 h post-mortem from eight (n = 8) Nellore bull carcasses, fabricated into 1.5-cm steaks, aerobically packaged, and stored at 4°C for nine days. Steaks were analyzed for myoglobin concentration, pH, instrumental color, metmyoglobin reducing activity (MRA) and lipid oxidation. Results: The LL steaks exhibited greater (p<0.05) redness, color stability, and MRA than their PM counterparts on days 5 and 9. The LL and PM steaks demonstrated similar (p>0.05) lightness and yellowness on days 0, 5, and 9. On the other hand, PM steaks exhibited greater (p<0.05) myoglobin concentration, pH, and lipid oxidation than their LL counterparts. Conclusion: These results indicated that muscle source influenced the color and oxidative stabilities of beef from grain-finished Bos indicus animals. These results highlighted the necessity of muscle-specific strategies to improve the color stability of beef from grain-fed Bos indicus cattle.

Analysis of the shelf life of chitosan stored in different types of packaging, using colorimetry and dentin microhardness

Antonio Miranda da Cruz-Filho,Angelo Rafael de Vito Bordin,Luis Eduardo Souza-Flamini,Débora Fernandes da Costa Guedes,Paulo César Saquy,Ricardo Gariba Silva,Jesus Djalma Pécora 대한치과보존학회 2017 Restorative Dentistry & Endodontics Vol.42 No.2

Objectives: Chitosan has been widely investigated and used. However, the literature does not refer to the shelf life of this solution. This study evaluated, through the colorimetric titration technique and an analysis of dentin micro-hardness, the shelf life of 0.2% chitosan solution. Materials and Methods: Thirty human canines were sectioned, and specimens were obtained from the second and third slices, from cemento-enamel junction to the apex. A 0.2% chitosan solution was prepared and distributed in 3 identical glass bottles (v1, v2, and v3) and 3 plastic bottles (p1, p2, and p3). At 0, 7, 15, 30, 45, 60, 90, 120, 150, and 180 days, the specimens were immersed in each solution for 5 minutes (n = 3 each). The chelating effect of the solution was assessed by micro-hardness and colorimetric analysis of the dentin specimens. 17% EDTA and distilled water were used as controls. Data were analyzed statistically by two-way and Tukey-Kramer multiple comparison (α = 0.05). Results: There was no statistically significant difference among the solutions with respect to the study time (p = 0.113) and micro-hardness/time interaction (p = 0.329). Chitosan solutions and EDTA reduced the micro-hardness in a similar manner and differed significantly from the control group (p < 0.001). Chitosan solutions chelated calcium ions throughout the entire experiment. Conclusions: Regardless of the storage form, chitosan demonstrates a chelating property for a minimum period of 6 months.

Identification and Characterization of a Class III Chitin Synthase Gene of Moniliophthora perniciosa, the Fungus That Causes Witches' Broom Disease of Cacao

Catiane S. Souza,Bruno M. Oliveira,Gustavo G. L. Costa,Albert Schriefer,Alessandra Selbach-Schnadelbach,Ana Paula T. Uetanabaro,Carlos P. Pirovani,Gonçalo A. G. Pereira,Alex G. Taranto,Júlio Cézar de 한국미생물학회 2009 The journal of microbiology Vol.47 No.4

Chitin synthase (CHS) is a glucosyltransferase that converts UDP-N-acetylglucosamine into chitin, one of the main components of fungal cell wall. Class III chitin synthases act directly in the formation of the cell wall. They catalyze the conversion of the immediate precursor of chitin and are responsible for the majority of chitin synthesis in fungi. As such, they are highly specific molecular targets for drugs that can inhibit the growth and development of fungal pathogens. In this work, we have identified and characterized a chitin synthase gene of Moniliophthora perniciosa (Mopchs) by primer walking. The complete gene sequence is 3,443 bp, interrupted by 13 small introns, and comprises a cDNA with an ORF with 2,739 bp, whose terminal region was experimentally determined, encoding a protein with 913 aa that harbors all the motifs and domains typically found in class III chitin synthases. This is the first report on the characterization of a chitin synthase gene, its mature transcription product, and its putative protein in basidioma and secondary mycelium stages of M. perniciosa, a basidiomycotan fungus that causes witches’ broom disease of cacao.

Final report on key comparison CCAUV.U-K3.1

Haller, J,Koch, C,Costa-Felix, R P B,Dubey, P K,Durando, G,KIM, Y T,Yoshioka, M BUREAU INTERNATIONAL DES POIDS ET MESURES 2016 METROLOGIA -BERLIN- Vol.53 No.-

<P></P> <P>The CCAUV.U-K3.1 is a follow-up of the comparison CCAUV.U-K3 which was the third key comparison in the ultrasound branch organised under the auspices of the Consultative Committee for Acoustics, Ultrasound and Vibration (CCAUV) of the BIPM. It was necessary to allow participants with discrepant values and additional laboratories to have another possibility to take part in an international comparison and to test their measurement facilities. The comparison dealt with power measurement in the frequency range 2 to 10.5 MHz and a power range between 10 mW and 15 W. The parameter range covered is of particular importance for medical applications of ultrasound.</P> <H2>Main text</H2> <P> To reach the main text of this paper, click on <A HREF='http://www.bipm.org/utils/common/pdf/final_reports/AUV/U-K3/CCAUV.U-K3.1_Final_Report.pdf'>Final Report</A>. Note that this text is that which appears in Appendix B of the BIPM key comparison database <A HREF='http://kcdb.bipm.org/'>kcdb.bipm.org/</A>.</P> <P>The final report has been peer-reviewed and approved for publication by the CCAUV, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).</P>

<i>δ</i> Sct-type pulsations in eclipsing binary systems: Y Cam

Rodrí,guez, E.,Garcí,a, J. M.,Costa, V.,Lampens, P.,van Cauteren, P.,Mkrtichian, D. E.,Olson, E. C.,Amado, P. J.,Daszyń,ska-Daszkiewicz, J.,Turcu, V.,Kim, S.-L.,Zhou, A. Y.,Ló,p Blackwell Publishing Ltd 2010 MONTHLY NOTICES- ROYAL ASTRONOMICAL SOCIETY Vol.408 No.4

<P>ABSTRACT</P><P>We present the results of a three-continent multisite photometric campaign carried out on the Algol-type eclipsing binary system Y Cam, in which the primary component is a multiperiodic δ Sct-type pulsator. The observations consist of 86 nights and more than 450 h of useful data collected mainly during the Northern winter 2002–2003. This means that this is the most extensive time series for such kind of systems obtained so far. These observations were collected mostly in the Johnson V filter, but they also include, for the first time, nearly complete binary light curves in simultaneous Strömgren <I>uvby</I> filters together with a few Crawford Hβ data obtained around the orbital phase of the first quadrature. A detailed photometric analysis is presented for both binarity and pulsation. The results indicate a semidetached system with the secondary filling its Roche lobe. No significant contribution from a third body is found. The residuals from the computed binary solution were then used to investigate the pulsational content of the primary component. The frequency analysis of the out-of-primary-eclipse data leads to a set of eight significant and independent pulsational peaks in a well-defined region of the frequency domain. This means that this is the largest set of excited modes discovered so far in the pulsating component of such kind of systems. The possibility of aliasing problems during the present run or short-term time-scale amplitude variations in some of them was investigated with null results. Indeed the results indicate that <I>f</I><SUB>1</SUB> and <I>f</I><SUB>3</SUB> form a frequency doublet with a beat period of <I>P</I><SUB>beat</SUB>= 17.065 d. Our results confirm the frequencies already detected by earlier authors and show the presence of some additional significant peaks. The observed amplitudes during the present run are also consistent with those derived from older data sets. We perform a preliminary mode identification for most of the frequencies on the basis of the collected multicolour photometry, the observed frequency spacings and the mode visibility in eclipsing binaries.</P>

Intake and Performance of Yearling Steers Grazing Guineagrass (Panicum maximum cv. Tanzânia) Pasture Supplemented with Different Energy Sources

Santana, M.C.A.,Euclides, V.B.P.,Mancio, A.B.,Medeiros, S.R.,Costa, J.A.R.,Oliveira, R.L. Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.3

The aim of this study was to evaluate the efficiency of supplements containing different energy sources in relation to mineral supplementation of steers grazing guineagrass (Panicum maximum cv Tanz$\hat{a}$nia) pasture, during the dry season. The experimental design was a randomized block with three treatments and four replications. The treatments consisted of a mineral supplementation and two other supplements, one based on corn seed and the other based on soybean hulls, and provided at 0.8% of body weight. Forty-eight, 12 month-old crossbred steers with an average initial body weight of 267 kg, were assigned to twelve paddocks (1,125 ha) of guineagrass. The animals that were fed with soybean hulls and corn seed presented a greater average daily gain (0.982 and 0.937) when compared with the mineral supplementation. Soybean hulls can be used as a satisfactory food source, replacing corn as an energy source in the supplementation of beef cattle without compromising animal performance.

Effect of ripening time on bacteriological and physicochemical goat milk cheese characteristics

Rodrigo V. Moreira,Marion P. Costa,Beatriz S. Frasao,Vivian S. Sobral,Claudius C. Cabral,Bruna L. Rodrigues,Se´rgio B. Mano,Carlos A. Conte-Junior 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.4

Cheese ripening involves lactose metabolism, lipolysis and proteolysis, which are affected by many factors. The aim of this study was to assess changes due to ripening (90 days) of goat milk cheese through bacteriological and physicochemical analysis in order to verify if, at the end of ripening period, this cheese could be considered ‘‘lactose-free’’. Three batches of the goat milk cheese were manufactured and ripened at 10 C and 80% relative humidity for 90 days. Titratable acidity increased by about 59 D due to carbohydrate degradation and organic acid production. However, pH (5.31–5.25) remained constant. Lactococcus was the dominant cheese microbiota, acting in the fermentation of lactose (1.17–0.06 mg/g) and lactic acid production (5.49– s10.01 mg/g). Thus, ripening time was decisive for bacteriological and physicochemical goat milk cheese characteristics.

Influence of shear deformation of exterior beam-column joints on the quasi-static behavior of RC framed structures

Ricardo J.T. Costa,Fernando C.T. Gomes,Paulo M.M.P. Providência,Alfredo M.P.G. Dias 사단법인 한국계산역학회 2013 Computers and Concrete, An International Journal Vol.12 No.4

In the analysis and design of reinforced concrete frames beam-column joints are sometimes assumed as rigid. This simplifying assumption can be unsafe because it is likely to affect the distributions of internal forces and moments, reduce drift and increase the overall load-carrying capacity of the frame. This study is concerned with the relevance of shear deformation of beam-column joints, in particular of exterior ones, on the quasi-static behavior of regular reinforced concrete sway frames. The included parametric studies of a simple sub-frame model reveal that the quasi-static monotonic behavior of unbraced regular reinforced concrete frames is prone to be significantly affected by the deformation of beam-column joints.

A facile ultrasound‑assisted synthesis of mesoporous carbon

Rayanne O. Araujo,Vanuza O. Santos,Jamily L. Santos,Flaviana C. P. Ribeiro,Maria J. F. Costa,Jamal S. Chaar,Newton P. S. Falc?o,Carlos E. F. da Costa,Luiz K. C. de Souza 한국탄소학회 2023 Carbon Letters Vol.33 No.1

The ultrasonic method is an alternative to the conventional route to produce structured carbon materials, offering the advantages of synthesis in a short period of time under room temperature. The main objective of this work is to synthesize a sulfonated mesoporous carbon catalyst from a phenolic resin composed of phloroglucinol and formaldehyde. The synthesis was performed by the soft-template method in an ultrasonic processor and the product was subsequently carbonized and sulfonated for application in the esterification model reaction. Functionalization with sulfuric acid of MCS5-6 h sample brought about a decrease in porosity but simultaneously resulted in the generation of functional groups of an acidic nature. The MCS5-6 h catalyst with a sulfonic density of 1.6 mmol g? 1, surface area of 402 m2 g? 1 and pore diameter of 10.6 nm maintained in mesoporous even after acid treatment. MCS5-6 h showed excellent activity in the esterification reaction with 95% oleic acid conversion. The recyclability of MCS5-6 h was satisfactory during five reaction cycles. The present work addressed a promising alternative for the synthesis of carbon catalysts using ultrasound irradiation, thus providing an alternative with a lower cost of time and energy for large-scale production.

Modeling of flow-induced crystallization of colored polypropylene in injection molding

Rong Zheng,R. I. Tanner,D. Lee Wo,Xi-jun Fan,C. Hadinata,F.S. Costa,P.K. Kennedy,P. Zhu,G. Edward 한국유변학회 2010 Korea-Australia rheology journal Vol.22 No.3

We present a study on flow-induced crystallization of an injection-molded isotactic polypropylene (iPP) mixed with colorant additives. Two types of blue colorants were used in the study: one is the ultramarine Blue composed of Sodium Alumino Sulpho Silicate (UB) and the other is the PV Fast Blue composed of Cu-Phthalocyanine (CuPc). The CuPc colorant exhibits increased nucleation of both quiescent and flow-induced crystallization, and results in more oriented microstructures, causing a high degree of anisotropy in material properties and shrinkage of the injection molded parts. In contrast, the nucleating effect of the UB colorant is negligible under the quiescent condition, but becomes notable in the case of short-term shear treatment at high shear rates. The UB colorant does not significantly increase the degree of anisotropy as compared with the virgin iPP. A flow-induced crystallization kinetic model is used in the numerical simulation for the injection molding process, and predicted the colorant effects as observed in experiments.