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김운기,Ok-Ju Sul,곽정숙,Hye-Young Hur,Anne M. Latour,Beverly H. Koller,권병세,Choon-Soo Jeong 생화학분자생물학회 2010 Experimental and molecular medicine Vol.42 No.12
Tumor necrosis factor receptor-related 2 (TR2, HVEM or TNFRSF-14) plays an important role in immune responses,however, the mechanisms regulating its expression are unclear. To understand the control of TR2gene expression, we studied the upstream region of the gene. Gel supershift assays revealed inducible binding of nuclear factor of activated T cells (NFAT)to a putative NFAT site within the TR2 promoter. Furthermore, cotransfection of a dominant negative NFAT construct, or siRNA for NFAT, resulted in increased expression of a TR2 reporter gene. Our findings demonstrate that NFAT negatively regulates TR2expression in activated T cells.
Overexpression of developmentally regulated GTP-binding protein-2 increases bone loss
Ke, Ke,Sul, Ok-Joo,Kim, Woon-Ki,Lee, Mi-Hyun,Ko, Myung-Seok,Suh, Jae-Hee,Kim, Hyun-Ju,Kim, Shin-Yoon,Park, Jeong-Woo,Choi, Hye-Seon American Physiological Society 2013 AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND M Vol.304 No.7
<P>The developmentally regulated GTP-binding protein-2 (DRG2) is a novel subclass of GTP-binding proteins. Many functional characteristics of osteoclasts (OC) are associated with small GTPases. We hypothesized that DRG2 affects bone mass via modulating OC activity. Using DRG2 transgenic mice, we investigated the role of DRG2 in bone remodeling. DRG2 overexpression caused a decrease in bone mass and an increase in the number and activity of OC in vivo. DRG2 overexpression increased fusion, spreading, survival, and resorption activity of OC in vitro<I>.</I> Downregulation of DRG2 by siRNA decreased fusion, spreading, and survival of OC, supporting the observations found in DRG2 transgenic OC. Transgenic mature OCs were larger, with actin rings and higher ERK, Akt, Rac1 and Rho activities than wild-type OCs. Inhibition of these proteins abolished the effects of DRG2 on formation of large OCs with actin rings, implying that DRG2 affects cytoskeleton reorganization in a Rac1/Rho/ERK/Akt-dependent manner. In summary, DRG2 is associated with survival and cytoskeleton organization of OC under influence of macrophage colony-stimulating factor, and its overexpression leads to elevated bone resorptive activity of OC, resulting in bone loss.</P>
새로운 섬유소분해 균주 Trichoderma sp. C-4에서 분리한 Endoglucanase (F-I-III)에 대한 연구
설옥주,정대균,한인섭,정춘수,Sul Ok Ju,Chung Dae Kyun,Han In Seob,Jeong Choon Soo 한국미생물학회 2005 미생물학회지 Vol.41 No.1
One of the endoglucanases, F-I-III, was purified from the culture filtrate of T. sp. C-4 through procedures including chromatography on Sephacryl S-200, DEAE-Sepharose A-50, and Chromatofocusing on Mono-P (FPLC). The molecular weight of the enzyme was determined to be about 56,000 Da by SDS-PAGE, and pI of 4.9 by analytical isoelectric focusing. F-I-III showed the highest enzyme activity at $55^{\circ}C$, and the pH optimum of the enzyme was 5.0. There was no loss of activity when the enzyme was incubated at $50^{\circ}C$ for 24 hours. The specific activity of the enzyme F-I-III toward the CMC was 315.4 U/mg. The Km value for $PNPG_2$ of F-I-III was 2.69 mM. N-terminal sequence of F-I-III was analyzed to be QPGTSTPEVHPKKLTTYK. It showed $95\%$ of homology to that of EGI from T. reesei. The presence of some metal ions (1 mM) had only a little effect on CMCase activity. The treatment of the reducing agents resulted in the increase of endoglucanase activity. 국내에서 분리된 우수섬유소분해 균주인 Trichodema sp. C-4가 생성하는 endoglucanase 중하나를 $(NH_4)_2SO_4$ 침전, Sephacryl S-200 gel filtration, DEAE-Sepharose A-50 ion exchange, Mono-P chromatofocusing (EPLC)의 단계로 정제하고 이를 F-I-III라 명명하였다. 분리된 효소 F-I-III는 분자량 56,000Da, 둥전점 4.9로 측정된 단일 단백질이었다. F-I-III는 $55^{\circ}C$에서 가장 높은 활성을 보였으며, pH 5.0이 반응 최적 조건이었다. $50^{\circ}C$에서 24시간 동안 안정하였으며, pH 4-7의 범위에서 안정하였다. CMC에 대한 비활성은 315.4U/mg 이었으며, PNPG2에 대한 Km 값은 2.69 mM이었다. 이 효소는 같은 균주에서 분리한 다른 endoglucanase와 exoglucanase를 섞었을 때 결정형 섬유소인 Avicel분해에 대한 상승효과를 보였다. $Mg^{2+},\;CO^{2+},\;Fe^{2+},\;Ca^{2+},\;CS^+,\;Li^+$ 등의 이온은 1 mM의 농도에서 효소의 활성에 큰 영향을 미치지 않았고, 1 mM의 환원제 (cystein, EDIA, \beta-mercaptoethanol, dithiothreitol(DTT), L-ascorbic acid)들은 효소의 활성을 증가시켰다. E-I-III의 N-말단 서열을 분석하여 QPGTSTPEVHPKKLTTYK의 서열을 얻었다. 이는 Trichodema reesei의 endoglucanase인 EGI과 $95\%$의 유사도를나타내었다. 분리된 효소 F-I-III는 높은 비활성을 가지고 있어서 활용가치가 높을 것으로 사료되었다.
김운기,Jin-Soo Park,Ok-Ju Sul,Jae-Hee Seo,최범규,Hee-Young Park,Anne M. Latour,Beverly H. Koller,권병세,Choon-Soo Jeong 한국분자세포생물학회 2011 Molecules and cells Vol.31 No.2
Previous work has suggested that the LIGHT-TR2 costimulatory pathway plays a role in the acute and chronic stages of dextran sulfate sodium (DSS)-induced colitis [Steinberg et al. (2008); Wang et al. (2005)]. To clarify the role of TNFR-related 2 (TR2) signaling in the maintenance of intestinal homeostasis, we generated a TR2 knock-out (KO) mouse. Using DSS to induce colitis, we compared the colitic symptoms and pathological changes in wild type (WT) and TR2 KO mice, and the production of cytokines by the diseased colons. We also studied the role of TR2 in suppressing innate and adaptive immunity in the DSS model. TR2 deficient mice were characterized by reduced symptoms of intestinal inflammation compared with wildtype mice, and reduced production of cytokines. We therefore generated a monoclonal antibody against mouse TR2 which was specific to TR2 and capable of blocking TR2 signals. With this antibody, we demonstrated that antagonizing TR2 during the development of DSS-induced colitis reduced the symptoms of inflammation. Our findings suggest that TR2 is an important mediator in colitis, and may serve as a therapeutic target in inflammatory bowel disease.
Kim, Woon-Ki,Sul, Ok-Ju,Kwak, Jung-Sook,Hur, Hye-Young,Latour, Anne M.,Koller, Beverly H.,Kwon, Byoung-S.,Jeong, Choon-Soo Korean Society for Biochemistry and Molecular Bion 2010 Experimental and molecular medicine Vol.42 No.12
Tumor necrosis factor receptor-related 2 (TR2, HVEM or TNFRSF-14) plays an important role in immune responses, however, the mechanisms regulating its expression are unclear. To understand the control of TR2 gene expression, we studied the upstream region of the gene. Gel supershift assays revealed inducible binding of nuclear factor of activated T cells (NFAT) to a putative NFAT site within the TR2 promoter. Furthermore, cotransfection of a dominant negative NFAT construct, or siRNA for NFAT, resulted in increased expression of a TR2 reporter gene. Our findings demonstrate that NFAT negatively regulates TR2 expression in activated T cells.
Kim, Seok-Hyung,Lee, Ok-Jun,Kwon, Ju-Lee,Kim, Jin Man,Sul, Hae-Joung,Song, Kyu Sang,Kim, Kyung-Hee Potamitis Press 2015 Anticancer research Vol.35 No.7
<P>To investigate whether differential expression of Yes-associated protein (YAP) and 관-catenin is important in gastric carcinogenesis.</P>
Ke, Ke,Kim, Woon-Ki,Sul, Ok-Joo,Phan, Van Tien,Lee, Mi-Hyun,Kim, Hyun-Ju,Kim, Shin-Yoon,Choi, Hye-Seon American Physiological Society 2012 AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND M Vol.303 No.11
<P>The aim of the present study was to evaluate the effect of fibrinogen on number and function of osteoclasts (OC) consequently resulting in bone loss. It was hypothesized that the enhanced level of released fibrinogen due to loss of ovarian function caused bone loss by acting on OCs. Bone loss was induced by ovariectomy (OVX) in mice and analyzed by micro-CT. The effect of fibrinogen on OCs was evaluated by tartrate-resistant acid phosphatase, annexin V, actin staining, pit formation observed on dentine slices, and Western blotting. Exogenous fibrinogen increased OC survival, actin ring formation, and bone resorption in vitro. The effect of fibrinogen was dependent on β(3)-integrin, which is a marker for mature OCs. Fibrinogen induced the activation of transforming oncogene from Ak strain (Akt), Ras-related C3 botulinum toxin substrate 1 (Rac1), and Rho family of GTPase (Rho) and the degradation of the Bcl-2 interacting mediator of cell death (Bim) in a manner similar to macrophage colony-stimulating factor (M-CSF). OVX increased plasma fibrinogen and serum M-CSF together with elevated actin ring formation and bone loss. The increased fibrinogen level due to loss of ovarian function may contribute, at least partly, to bone loss through the enhanced number and activity of OCs.</P>
분체공학 , 유동층 , 고분자 , 재료 ( 무기 , 유기 ) : 소결금속섬유필터의 기초 성능 특성
김상도(Sang Do Kim),박영옥(Young Ok Park),서정민(Jeong Min Seo),박석주(Seok Ju Park),최호경(Ho Kyoung Choi),박현설(Hyun Sul Park),임정환(Jeong Hwan Lim),손재익(Jae Ek Son) 한국화학공학회 2001 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.39 No.4
Basic filtration characteristics including pressure drop, penetration and fractional collection efficiency were investigated for sintered metal fiber filters. Two filters were applied. It was found that the porosity, weight and thickness were same, the air permeability and filtering accuracy were different. The performance test of fabric filter was investigated using a flat sheet filter testing unit, which can be applied to the filter of 300×300 ㎜ size. The test dust was AC Fine Dust. The pressure drop increased with filtration velocity and dust loading. The initial stage of dust cake formation was a growing process of particle dendrites on a single filament in the pore and the second stage was a bridge formation process with increasing dust loading. The final stage was a process of constant deposition of dust on a fabric surface under heavy dust loading conditions. The penetration sharply increased with filtration velocity in the intial stage of dust loading. It sharply decreased with dust loading, but the rate of decrease gradually diminished under heavy dust loading conditions. The pressure drop and penetration have been correlated in terms of operating variables such as dust loading and filtration velocity. The pressure drop and penetration calculated from the correlation equation have been compared with the experimental data and the agreement between the two data was fairly good. The fractional collection efficiency increased with dust loading.
이술현,손미경,박지일,김옥수,정현주,김영준,Lee, Sul-Hyun,Son, Mee-Kyoung,Park, Ji-Il,Kim, Ok-Su,Chung, Hyun-Ju,Kim, Young-Joon 대한치주과학회 2008 Journal of Periodontal & Implant Science Vol.38 No.4
Purpose: Autogenous transplantation of teeth can be defined as transplantation of teeth from one site to another in the same individual, involving transfer of impacted or erupted teeth into extraction sites or surgically prepared sockets". Successful autogenous transplantation of teeth depends upon a complex variety of factors. Such factors include damage to the periodontal ligament of the donor tooth, residual bone height of the recipient site, extra-oral time of tooth during surgery. Schwartz and Andreasen previously reported that autogenous transplantation of teeth with incomplete root formation demonstrated higher success rate than that of teeth with complete root formation. Gault and Mejare yielded similar rate of successful autogenous transplantation both in teeth with complete root formation and in teeth with incomplete root formation when appropriate cases were selected. This case report was aimed at the clinical and radiographic view in autogenous transplantation of teeth with complete root formation. Materials and Methods: Patients who presented to the department of periodontics, Chonnam National University Hospital underwent autogenous transplantation of teeth. One patient had vertical root fracture in a upper right second molar and upper left third molar was transplanted. And another patient who needed orthodontic treatment had residual root due to caries on upper right first premolar. Upper right premolar was extracted and lower right second premolar was transplanted. Six months later, orthodontic force was applied. Results: 7 months or 11/2 year later, each patient had clinically shallow pocket depth and normal tooth mobility. Root resorption and bone loss were not observed in radiograph and function was maintained successfully. Conclusion: Autogenous transplantation is considered as a predictive procedure when it is performed for the appropriate indication and when maintenance is achieved through regular radiographic taking and follow-up.