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Nguyen, Khanh Hoang Viet,Dao, Trong Khoa,Nguyen, Hong Duong,Nguyen, Khanh Hai,Nguyen, Thi Quy,Nguyen, Thuy Tien,Nguyen, Thi Mai Phuong,Truong, Nam Hai,Do, Thi Huyen Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.5
Objective: Fibronectin 3 (FN3) and immunoglobulin like modules (Ig) are usually collocated beside modular cellulase catalytic domains. However, very few researches have investigated the role of these modules. In a previous study, we have sequenced and analyzed bacterial metagenomic DNA in Vietnamese goats' rumen and found that cellulase-producing bacteria and cellulase families were dominant. In this study, the properties of modular cellulases and the role of a FN3 in unique endoglucanase belonging to glycosyl hydorlase (GH) family 5 were determined. Methods: Based on Pfam analysis, the cellulases sequences containing FN3, Ig modules were extracted from 297 complete open reading frames (ORFs). The alkaline, thermostability, tertiary structure of deduced enzymes were predicted by AcalPred, TBI software, Phyre2 and Swiss models. Then, whole and truncated forms of a selected gene were expressed in Escherichia coli and purified by His-tag affinity column for assessment of FN3 ability to enhance enzyme activity, solubility and conformation. Results: From 297 complete ORFs coding for cellulases, 148 sequences containing FN3, Ig were identified. Mostly FN3 appeared in 90.9% beta-glucosidases belonging to glycosyl hydrolase family 3 (GH3) and situated downstream of catalytic domains. The Ig was found upstream of 100% endoglucanase GH9. Rarely FN3 was seen to be situated downstream of X domain and upstream of catalytic domain endoglucanase GH5. Whole enzyme (called XFN3GH5 based on modular structure) and truncate forms FN3, XFN3, FN3GH5, GH5 were cloned in pET22b (+) and pET22SUMO to be expressed in single and fusion forms with a small ubiquitin-related modifier partner (S). The FN3, SFN3 increased GH5 solubility in FN3GH5, SFN3GH5. The SFN3 partly served for GH5 conformation in SFN3GH5, increased modules interaction and enzyme-soluble substrate affinity to enhance SXFN3GH5, SFN3GH5 activities in mixtures. Both SFN3 and SXFN3 did not anchor enzyme on filter paper but exfoliate and separate cellulose chains on filter paper for enzyme hydrolysis. Conclusion: Based on these findings, the presence of FN3 module in certain cellulases was confirmed and it assisted for enzyme conformation and activity in both soluble and insoluble substrate.
Hong Khanh Dieu Nguyen,Toan Dang Nguyen,Dung Ngoc Hoang,Duc Sy Dao,Thao Tien Nguyen,Limphirat Wanwisa,Lan Linh Hoang 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.2
Hydrotalcite-like compound containing metal cations such as Mg2+, Al3+ and Ni2+ was characterized using Ni K-edge EXAFS and in situ Ni K-edge XANES techniques for clarifying its bonding environment around Ni2+ sites and structure changes during calcination from room temperature to 550 oC, respectively. At the fixed molar ratio of Mg/Ni/Al of 2/1/1, the results obtained from EXAFS analysis showed a slight blue shift before and after the calcination at 550 oC and a reduction in white line peak; the best fits of the two samples revealed tiny change in coordination number about 7 for Ni-O path but considerable difference for Ni-Mg(Al) path from about 4.5 to 9.5, confirming a modification from brucite like to mixed oxide structure. On the other hand, bond distances of the Ni-O and Ni-Mg paths nearly fixed at about 2.06 Å to 3.0 Å reflected stability of the cationic bond order on each plane, but partial collapse and decomposition of the interlayer formed by water molecules and anion CO3 2− after the calcination. Linear combination fit extracted from the in situ Ni K-edge XANES also confirmed the changes along with the calcination such as slow and fast decreases of brucite fraction at 150 oC and 330 oC, respectively, in corresponding to the mixed oxide fraction increases. The achieved bonding structures were also applied to explain acid-base occurrence of the hydrotalcite-like material, especially the acid sites generated by different static charges along with the bonds. The explanation was illustrated by NH3-TPD method.
Advanced Magnetic Materials Produced by Using Rapid Quenching Technology
Nguyen Hoang Nghi,Nguyen Van Dung,Trinh Thi Thanh Nga,Bui Thi Khanh Nhung,Mai Thanh Tung,Nguyen Huu Tinh,Hoang Nhat Hieu,Bui Xuan Chien,Nguyen Thi Hong Tam 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.6
Amorphous alloys produced by using a rapid quenching technique are considered to be a precursor for producing nanosized and multiphase structures by precipitation (the top-down method). By using this technique, several new magnetic materials with different magnetic properties based on different physical mechanisms have been manufactured and investigated. Three new magnetic materials, a nanocrystalline soft magnetic material, a soft-hard permanent magnet and a Me-3d granular alloy with a GMR effect, what were prepared under local conditions, are presented and discussed in the experimental point of view. Amorphous alloys produced by using a rapid quenching technique are considered to be a precursor for producing nanosized and multiphase structures by precipitation (the top-down method). By using this technique, several new magnetic materials with different magnetic properties based on different physical mechanisms have been manufactured and investigated. Three new magnetic materials, a nanocrystalline soft magnetic material, a soft-hard permanent magnet and a Me-3d granular alloy with a GMR effect, what were prepared under local conditions, are presented and discussed in the experimental point of view.
Nguyen Thi Hong Chien,Thi Lan Nguyen,Khanh Linh Bui,Tho Van Nguyen,Thanh Hoa Le 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.1
Anaplasma marginale and A. platys were detected and characterized (16S rDNA sequence analysis) from dairy and indigenous cattle, and the latter in domestic dogs in Vietnam. A phylogenetic tree was inferred from 26 representative strains/species of Anaplasma spp. including 10 new sequences from Vietnam. Seven of our Vietnamese sequences fell into the clade of A. marginale and 3 into A. platys, with strong nodal support of 99 and 90%, respectively. Low genetic distances (0.2-0.4%) within each species supported the identification. Anaplasma platys is able to infect humans. Our discovery of this species in cattle and domestic dogs raises considerable concern about zoonotic transmission in Viet- nam. Further systematic investigations are needed to gain data for Anaplasma spp. and members of Anaplasmataceae in animal hosts, vectors and humans across Vietnam.
The Impact of Exchange Rate on Exports and Imports: Empirical Evidence from Vietnam
Nga Hong NGUYEN,Hat Dang NGUYEN,Loan Thi Kim VO,Cuong Quoc Khanh TRAN 한국유통과학회 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.5
The exchange rate is considered a tool improving the volume of exports and reducing imports. This paper aims to determine the impact of the exchange rate on exports and imports between Vietnam and the United States in the context of the trade war. The research uses Autoregressive Distributed Lag (ARDL) and Nonlinear Autoregressive Distributed Lag (NARDL) Model in the time-series data from 2010:1 to 2020:9. The ARDL’s results support that real exchange rate impact on export and import volumes, but less than the trade war. The trade war helps trade balance increase 0.35%, while the exchange rate increases trade balance 0.191% when the Vietnamese currency devalues 1% in the long run. In the short term, the real exchange rate makes the trade balance decrease. Therefore, the J curve exists between Vietnam and the U.S. The NARDL expresses that the exchange rate is asymmetric both in the short term and the long term. The findings of this study point to two important elements. Firstly, the exchange rate plays a minor role in exports and imports. Secondly, trade war plays a vital role in increasing exports and imports volume between two countries, and the J curve exists between the two countries.
Effect of Saline Concentrations on Biological Nitrification in Batch Reactor
Young Joon Lee, Nguyen Viet Hoang, Nguyen Hong Khanh, Pham Tuan Linh, Gi Youn Kim 조선대학교 기초과학연구원 2011 조선자연과학논문집 Vol.4 No.2
Pham Tuan Linh2 and Gi Youn Kim This study was carried out on 4 batch reactors to determine the specific ammonium oxidizing rate (SAOR), specific nitrate forming rate (SNFR) and inhibitory degree of nitrifying activities with saline concentrations. Under salt free condition ammonia was consumed during the reaction period within 200 min. When the salt level increased to 10, 20 and 30 g NaClL-1 in reactor, ammonia depletion took 250, 300 and above 350 min, respectively. During concentration above 10 g NaClL-1, there was nitrite accumulation. Also, at 30 g NaClL-1 ammonia did not depleted and NO2 --N accumulated until the final reaction. Nitrate formation rates decreased with increasing salt concentration. SAOR and SNFR showed a decreasing trend as salinity concentrations were increased. The SAOR was reduced from 0.2 to 0.08 mg NH4+-N g-1VSS day-1 as the salt concentration increased from 0 to 30 g NaCLL-1. Similarly, the SNFR decreased from 0.26 kg NO3--N kg-1VSS day-1 at saline free to 0.1 kg NO3--N kg-1VSS day-1 at saline 30 g L-1. A severe inhibition of nitrifiers activity was observed at increased salt concentrations. The inhibition ratio of specific ammonium oxidation rates were 17, 47 and 60% on the reactor of 10, 20 and 30 g NaClL-1 added, respectively. The inhibition ratio of specific nitrate forming rates also were inhibited 30, 53 and 62% on the reactor of 10, 20 and 30 g NaClL-1 added, respectively. As the salinity concentrations increased from 0 to 30 mg NaClL-1, the average MLSS concentration increased from 1,245 to 1,735 mgL-1. The SS concentration of supernatant in reactor which settled about 30 minutes was not severely difference between concentration of salt free reactor and one of those high salt contained reactors
MAI, Thi Cam Tu,NGUYEN, Hong Son,PHAN, Nguyen Ngoc Diem,LE, Minh Hang,LUU, Phuong Khanh,NGUYEN, Thi Thu Thao,NGUYEN, Thi Thu Trang Korea Distribution Science Association 2022 The Journal of Asian Finance, Economics and Busine Vol.9 No.6
This study examines the impact of CSR and its authenticity on customer loyalty through the brand image in the chain coffee shop industry. Using qualitative and quantitative methods, 601 survey questionnaires were distributed, of which 491 were analyzed. The results show that CSR has a positive impact on brand image and customer loyalty. In addition, authenticity does not moderate the impact of CSR on brand image and loyalty, as it is difficult for customers to verify the authenticity of CSR action programs. This study emphasizes the importance of CSR action programs and authenticity for businesses to sustainably enhance their distinctive brand image and customer loyalty. Therefore, for sustainable development in the future, managers of coffee shop chains need to focus on the following issues. First, the most important thing is the right awareness of businesses regarding CSR and CSR authenticity. Third, businesses should strengthen the transparency of CSR action programs in various media so that consumers can easily verify authenticity, to increase brand image and improve customer loyalty.