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Engineering lead-sensing GFP through rational designing
Nadarajan, Saravanan Prabhu,Ravikumar, Yuvaraj,Deepankumar, Kanagavel,Lee, Chong-Soon,Yun, Hyungdon The Royal Society of Chemistry 2014 Chemical communications Vol.50 No.100
<P>Lead is one of the most hazardous metals ubiquitous in the environment, causing serious health hazards to organisms. Recently, fluorescent proteins such as GFP and Dsred were utilized for the development of reagent-less rapid metal sensors. Here, we demonstrate the development of a lead-sensing GFP that is highly sensitive to lead at micro molar concentrations.</P> <P>Graphic Abstract</P><P>A lead biosensor (PbGFP) was developed by engineering lead binding site near the chromophore of green fluorescent protein. The specific binding of lead to chromophore of PbGFP resulted in turn-off mechanism. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c4cc07163h'> </P>
Mathur Nadarajan Kathiravan,김근호,류재원,김평일,이철원,김시욱 한국미생물학회 2015 The journal of microbiology Vol.53 No.11
In this study, novel DNA extraction and purification methods were developed to obtain high-quantity and reliable quality DNA from the microbial community of agricultural yellow loess soil samples. The efficiencies of five different soil DNAextraction protocols were evaluated on the basis of DNA yield, quality and DNA shearing. Our suggested extraction method, which used CTAB, EDTA and cell membrane lytic enzymes in the extraction followed by DNA precipitation using isopropanol, yielded a maximum DNA content of 42.28 ± 5.59 μg/g soil. In addition, among the five different purification protocols, the acid-treated polyvinyl polypyrrolidone (PVPP) spin column purification method yielded high-quality DNA and recovered 91% of DNA from the crude DNA. Spectrophotometry revealed that the ultraviolet A260/A230 and A260/A280 absorbance ratios of the purified DNA were 1.82 ± 0.03 and 1.94 ± 0.05, respectively. PCR-based 16S rRNA amplification showed clear bands at ~1.5 kb with acid-treated PVPP–purified DNA templates. In conclusion, our suggested extraction and purification protocols can be used to recover high concentration, high purity, and high-molecular-weight DNA from clay and silica-rich agricultural soil samples.
( Krithikaa Nadarajan ),( Mei Ling Kang ) 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1
Case report: A previously healthy 36 year old male presented with diffi culty passing urine after sexual intercourse. This was associated with fever and dysuria. On examination, he was febrile at 37.9 degrees but did not appear septic. There was no loin tenderness, palpable bladder or prostatic tenderness; other systems were normal. He had raised white count of 16,000 (90% polys) and procalcitonin of 23.7. Urine microscopic examination showed pyuria. Blood cultures isolated gram-positive cocci in clusters. He was given empirical vancomycin to cover for Staph. Aureus and Coagulase-Negative Staphylococci. The gram-positive organism was subsequently identifi ed as Aerococcus Urinae. Aerococcus Urinae, fi rst described in 1992, as a catalase-negative environmental Gram-positive coccus growing in clusters; and colonizer of the urinary tract, is increasingly reported to cause urinary tract infections. It can also cause invasive infections such as bacteremia and infective endocarditis. Due to its morphology, it is often misidentifi ed as staphylococci. On blood agar it causes alpha hemolysis and may also be mistaken for Streptococcus viridans. It also shares similar antibiotic resistance patterns as Enterococci Spp and may be misidentifi ed as such. This has therapeutic implications. We review and discuss the infections caused by Aerococcus Urinae, its diagnosis and management.
Mathur Nadarajan Kathiravan,김근호,류재원,한기환,김시욱 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.2
A novel hexavalent chromium (CrVI)-removing Bacillus sp. was isolated from leather industry wastewatercontaminated soil. This potential isolate was subjected to Cr(VI) removal under free and immobilized states in a stirred batch reactor (SBR). Two biokinetic parameters, Vmax and Km, and the effective diffusivity (De) for various bead sizes were calculated from Lineweaver-Burk and Eadie-Hoftsee plots, respectively. With respect to bead size, De decreased significantly from a maximum of 3.024 × 10−6 cm2/sec in the 0.20 cm bead to 2.948 × 10−6, 1.775 × 10−7, and 1.144 × 10−7 cm2/sec in the 0.40-, 0.60- and 0.80 cm beads, respectively. Additionally, steady and unsteady state modeling of diffusional mass transfer into the immobilized beads was conducted to determine the mass transfer rate as a function of time and the beads’ radial profile. Furthermore, the space-time yield (STY) was modeled according to the residence time of the reactor. The reactor’s STY was reasonable and could be further boosted by increasing the fractional biocatalyst.