Poster Session : PS 0377 ; Infectious Disease ; Multi-Year Vancomycin Course is Feasible in the Outpatient Parenteral Antimicrobial Therapy (OPAT) Clinic

( Mei Ling Kang ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Vancomycin remains the mainstay of treatment for methicillin-resistant Staphylococcus aureus (MRSA) infections despite reports of decreasing effi cacy and advent of newer agents. Prolonged use of vancomycin is limited by intravenous (iv) formulation and concerns of toxicities. We describe 4 patients who, intolerant of alternative treatments, received 2 to 7 years of vancomycin in OPAT clinic with successful suppression of MRSA prosthetic infections. Case 1: Sixty-nine year-old man with infected spinal implant tolerated 29 months of vancomycin without renal, neurological or bone marrow toxicity, till removal of the implant. He suffered one episode of line-related bacteraemia 8th month into treatment and was successfully treated with line-change and antibiotics. Case 2: Seventy-one year old male with chronic osteomyelitis. He received 2 years of vancomycin via a peripherally inserted central catheter (PICC); and suffered renal impairment and PICC-related bacteraemia. He recovered following treatment with no long-term sequelae. Case 3: Seventy-one year-old woman with prosthetic hip infection was suppressed with 28 months of vancomycin. She did not experience neurological or haematological toxicities but had 2 episodes of PICC infections. One was mild; the other resulted in klebsiella bacteraemia and renal impairment. Both were successfully treated. Case 4: Sixty-seven year-old man with MRSA aortic graft infection received a 7-year course of Vancomycin in OPAT clinic. He developed permanent but mild renal impairment and thrombocytopenia. He also had PICC-related complications: chronic venous thrombosis requiring angioplasty and anticoagulation therapy 1 ½ years into treatment; and 2 episodes of line sepsis 7th year of vancomycin. His infection was successfully treated in hospital but he died of a myocardial infarction. Prolonged courses of vancomycin can be given in OPAT clinic with acceptable complications. Renal impairment is mostly mild and reversible. Line infections are common but treatable.

구조물 형상 추정을 위한 변위계측 위치 선정기법 연구

강미령(Jinag, Mei Ling),최준호(Choi, Jun Ho),김대혁(Kim, Dae Hyuk),이기세(Lee, Kee Sei),강영종(Kang, Young Jong) 대한토목학회 2012 대한토목학회 학술대회 Vol.2012 No.10

Poster Session : PS 0416 ; Infectious Disease ; Gram-Positive Cocci in Clusters in Blood Culture: It Ain`t Always Staphyloccocal Spp! A Review of Aerococcus Urinae Infections

( Krithikaa Nadarajan ),( Mei Ling Kang ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Case report: A previously healthy 36 year old male presented with diffi culty passing urine after sexual intercourse. This was associated with fever and dysuria. On examination, he was febrile at 37.9 degrees but did not appear septic. There was no loin tenderness, palpable bladder or prostatic tenderness; other systems were normal. He had raised white count of 16,000 (90% polys) and procalcitonin of 23.7. Urine microscopic examination showed pyuria. Blood cultures isolated gram-positive cocci in clusters. He was given empirical vancomycin to cover for Staph. Aureus and Coagulase-Negative Staphylococci. The gram-positive organism was subsequently identifi ed as Aerococcus Urinae. Aerococcus Urinae, fi rst described in 1992, as a catalase-negative environmental Gram-positive coccus growing in clusters; and colonizer of the urinary tract, is increasingly reported to cause urinary tract infections. It can also cause invasive infections such as bacteremia and infective endocarditis. Due to its morphology, it is often misidentifi ed as staphylococci. On blood agar it causes alpha hemolysis and may also be mistaken for Streptococcus viridans. It also shares similar antibiotic resistance patterns as Enterococci Spp and may be misidentifi ed as such. This has therapeutic implications. We review and discuss the infections caused by Aerococcus Urinae, its diagnosis and management.

A comparative study of the adjuvanticity of Hansenula polymorpha, Saccharomyces cerevsiae and Yarrowia lipolytica in oral and nasal immunization with virus capsid antigens

Lee, Jae Young,Xu, Mei Ling,Kim, Hyoung Jin,Kang, Hyun Ah,Kim, Hong-Jin Springer-Verlag 2013 Biotechnology letters. Vol.35 No.11

Anti-inflammatory effects of novel <i>polygonum multiflorum</i> compound via inhibiting NF-κB/MAPK and upregulating the Nrf2 pathways in LPS-stimulated microglia

Park, Sun Young,Jin, Mei Ling,Kang, Nam Jun,Park, Geuntae,Choi, Young-Whan Elsevier/North-Holland 2017 Neuroscience letters Vol.651 No.-

<P><B>Abstract</B></P> <P>The incorporation of <I>Polygonum multiflorum</I> into the diet can result in anti-aging effects owing to its wide range of biological and pharmaceutical properties. We investigated the anti-neuroinflammatory properties of CRPE56IGIH isolated from <I>P. multiflorum</I> by focusing on its role in the induction of phase II antioxidant enzymes and the modulation of upstream signaling pathways. In microglia, CRPE56IGIH significantly inhibited lipopolysaccharide (LPS)-stimulated nitric oxide and prostaglandin E<SUB>2</SUB> production with nonspecific cytotoxicity. CRPE56IGIH also markedly inhibited LPS-inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 protein and mRNA expression in the same manner as it inhibited nitric oxide and prostaglandin E<SUB>2</SUB> production. In the control cells, NF-κB transactivation and nuclear translocation occurred at a baseline level, which was significantly increased in response to LPS. However, pretreatment with CRPE56IGIH concentration-dependently inhibited the LPS-induced NF-κB transactivation and nuclear translocation. The phosphorylation of Janus kinase-signal transducers and activators of transcription and mitogen-activated protein kinases was markedly upregulated by LPS, but considerably and dose-dependently inhibited by pretreatment with CRPE56IGIH. Furthermore, CRPE56IGIH induced the expression of phase II antioxidant enzymes, including heme oxygenase-1 (HO-1) and NADPH dehydrogenase quinone-1 (NQO-1). The activation of upstream signaling pathways, such as the Nrf2 pathway, was significantly increased following CRPE56IGIH treatment. Furthermore, the anti-neuroinflammatory effect of CRPE56IGIH was reversed by transfection of Nrf2, HO-1, and NQO-1 siRNA. Our results indicated that CRPE56IGIH isolated from <I>P. multiflorum</I> could be used as a natural anti-neuroinflammatory agent that induces phase II antioxidant enzymes <I>via</I> Nrf2 signaling.</P> <P><B>Highlights</B></P> <P> <UL> <LI> CRPE56IGIH is a natural compound isolated from <I>Polygonum multiflorum.</I>. </LI> <LI> CRPE56IGIH inhibits LPS induced neuroinflammatory response in microglia. </LI> <LI> CRPE56IGIH inhibits LPS-induced NF-κB and JAK-STATs activation in microglia. </LI> <LI> Nrf2 mediates the anti-neuroinflammation of CRPE56IGIH in microglia. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Genetic Overlap in the Quantitative Resistance of Rice at the Seedling and Adult Stages to Xanthomonas oryzae pv. oryzae

Yong-Li Zhou,Xue-Wen Xie,Mei-Rong Xu,Jin-Ping Zang,Ling-Hua Zhu,Jian-Long Xu,Zhi-Kang Li 한국식물학회 2012 Journal of Plant Biology Vol.55 No.2

The genetic components responsible for the qualitative and quantitative resistance of rice to three Chinese races (C2, C4, and C5) of Xanthomonas oryzae pv. oryzae (Xoo)were investigated at the seedling and adult stages in two successive years in set of Lemont/Teqing cross introgression lines (ILs) in a Teqing background, to create a complete linkage map using 160 well-distributed SSR markers. Teqing was resistant to C2 and C4, but moderately susceptible to C5,whereas Lemont was susceptible to all three races. Highly significant correlations were detected among the resistance to different races at different developmental stages. A major gene (Xa4), 14 main-effect QTLs (M-QTLs), and 18 epistatic QTLs were identified in the two developmental stages over 2 years,and were largely responsible for the segregation of resistance in the ILs. In 2007, the Lemont alleles at all loci in the seedling stage, except QBbr10 to C4, increased lesion length (LL) or decreased resistance. The Teqing allele at the Xa4 locus acted as a resistance gene against C2 and C4, but acted as a M-QTL when its resistance was overcome by the virulent race C5. MQTLs showed a degree of race specificity and had a cumulative effect on resistance. Most M-QTLs (94%) consistently expressed resistance to the same race at the seedling and adult stages, indicating that a high degree of genetic overlap exists between Xoo resistance at both developmental stages in rice. Among the digenic interactions, most co-introgressed Lemont alleles at the two epistatic loci lead to significantly smaller LL with all three races, compared to other types of interacting alleles at both development stages. The results indicate that a high level of resistance may be achieved by the cumulative effect of multiple M-QTLs, including the residual effects of “defeated” major resistance genes and the epistatic effects of co-introgression from diverse susceptible varieties.