한우 c-fos 유전자의 염기서열 및 발현분석

유성란,정행진,정기철,이준헌,조규완,최재관,나기준,상병찬 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.6

Cellular FOS(c-fos) protein is a transcription factor that forms heterodimers mostly with c-jun family and stimulates the transcription of genes containing AP-1 regulatory elements. This c-fos expression can control growth and differentiation of various precursor cells including myoblasts. The controls by c-fos gene have been identified for affecting skeletal muscle fiber traits which are the key determinants of meat quality in pigs. As a first step for identifying the relationship between c-fos gene and meat quality traits in cattle, we fully sequenced 1,443 bp of Hanwoo c-fos mRNA and analyzed expression patterns from various organs and muscle tissues. The sequence identities of Hanwoo c-fos with that of human, pig and mouse showed 89.8%, 93.3% and rib muscle from 7 organs and 9 different parts of muscles investigated. These results presented here can be used as a valuable marker for meat quality related traits in cattle with further verification.

난관배양액이 처녀발생유기된 돼지난포란의 체외발달에 미치는 영향

문승주,이경호,김호,김창렬,은대숙,김광현,나진수,김재홍 全南大學校 農業科學技術硏究所 1997 農業科學技術硏究 Vol.32 No.-

본 연구는 난관배양액이 돼지수정란의 체외발달에 미치는 효과를 규명키 위하여 수행하였다. 돼지 미성숙 난포란은 TCM-199, Ham's F-10 그리고 Whitten's 배양액에 10% 난포액과 호르몬(PMSG : 10IU/㎖, HCG : 10IU/㎖)을 첨가 20시간 배양하고 호르몬을 첨가하지 않는 배양액에서 20시간 추가 배양하여 총 40시간동안 배양하여 체외성숙을 유도하였다. 체외성숙후 0.1% hyaluronidase로 난구세포를 제거하고 15% FCS가 함유된 TCM-199으로 3회 세척하고 TCM-199에 15% FCS와 10% ethanol 혼합액에 세척한 난자를 옮겨 10분간 배양 처녀발생을 유기하였다. 처녀발생 6시간후 전핵형성율은 체외성숙배양액으로 TCM-199을 사용했을 때 56.4%, Ham's F-10의 경우 58.3%, Whitten's 배양액의 경우 74.0%를 보였다. 처녀발생 유기 48시간째 난할율은 TCM-199을 사용했을 때 45.7%, Ham's F-10에서 45.4%, Whitten's배양액에서 39.2%를 보였으며 세종류의 배양액에 POCM을 첨가 배양했을 때 TCM-199에 44.8%, Ham's F-10에서 45.4%, Whitten's배양액에서 43.7%로 나타났다. 처녀발생육 난자를 96시간 체외배양시킨 결과 상실배 발달율이 POCM을 첨가 했을 때 첨가하지 않은 시험구에 비하여 유의적으로 높았다(P<0.05) The effect of porcine oviductal conditioned medium(POCM) on in vitro development of chemically activated porcine oocytes was studied. Porcine oocytes were cultured in TCM-199, Ham's F-10 and Whitten's medium with hormonal supplements for 20h and 40h additional culture without hormonal supplements. After in vitro maturation, the denuded oocytes were washed 3 times with TCM-199 contaning 15%(v/v) ethanol to induce pathenogenetic activation. At 6h after activation, pronuclea formation rates were 56.4% in TCM-199, 59.3% in Ham's F-10 and 74.0% in Whitten's maturation medium. At 48h after activation, 45.7%, 45.4% and 39.2% of oocytes claved in TCM-199, Ham's F-10 and Whitten's culture medium, respectively. And 44.8%, 45.5% and 43.7% of oocytes were claved in TCM-199, Ham;s F-10 and Whitten's culture medium supplemented with POCM, respectively. The rates of moular were higher in culture medium with POCM than without POCM at 96h after activation.(P<0.05)

Mechanical properties of (W,Ti)C and (W,Ti)C-NiAl₃ cermet consolidated by the high-frequency induction-heating method

Kim, W.,Suh, C.Y.,Roh, K.M.,Cho, S.W.,Na, K.I.,Shon, I.J. Elsevier Sequoia 2013 Journal of alloys and compounds Vol.568 No.-

In the case of cemented (W,Ti)C, Co is added as a binder for the formation of composite structures. However, the high cost of Co and the low corrosion resistance of the (W,Ti)C-Co cermet have generated interest in recent years for alternative binder phases. In this study, NiAl<SUB>3</SUB> was used as a binder and consolidated by the high-frequency induction heated sintering (HFIHS) method. The densification of both monolithic (W,Ti)C and (W,Ti)C-NiAl<SUB>3</SUB> cermet was accomplished within 3min. Highly dense (W,Ti)C and (W,Ti)C-NiAl<SUB>3</SUB> with a relative density of upto 99% were obtained within 3min by HFIHS under a pressure of 80MPa. The method was found to enable not only the rapid densification but also the prohibition of grain growth preserving the nano-scale microstructure. The average grain sizes of the sintered (W,Ti)C and (W,Ti)C-NiAl<SUB>3</SUB> were lower than 100nm. The addition of NiAl<SUB>3</SUB> to (W,Ti)C enhanced the toughness at the expense of the slight decrease in hardness. The hardness of (W,Ti)C and (W,Ti)C-NiAl<SUB>3</SUB> was significantly higher than that of (W,Ti)C-Co or (W,Ti)C-Ni. The fracture toughness and hardness values of (W,Ti)C, (W,Ti)C-5vol.%NiAl<SUB>3</SUB>, and (W,Ti)C-10vol.%NiAl<SUB>3</SUB> consolidated by HFIHS with a pressure of 80MPa and a induced current were 7.6+/-0.4MPam<SUP>½</SUP> and 2850+/-35kg/mm<SUP>2</SUP>, 8.5+/-0.3MPam<SUP>½</SUP> and 2610+/-37kg/mm<SUP>2</SUP>, 9.7+/-0.5MPam<SUP>½</SUP> and 2520+/-26kg/mm<SUP>2</SUP>, respectively.

조직구성 수성 망상증 1예

나한식,조경상,김종두,이승일,문철웅,이호영,장숙진 朝鮮大學校 附設 醫學硏究所 1989 The Medical Journal of Chosun University Vol.14 No.2

Histiocytic medullary reticulosis is a clinicopathologic syndrome characterized by its acute onset and relentless progression to death within a few months. The major clinical features are fever, malaise, weakness, weight loss, lymphadenopathy, hepatosplenomegaly, jaundice and purpura. The common laboratory findings are severe anemia, leukopenia, and thrombocytopenia. Histologically, bone marrow were infiltrated with atypical, neoplastic histiocytes, many of which had ingested large number of red blood cells, leukocyte, platelet. We experienced one case of HMR who was a 22 year old male admitted via ER with malaise, general weakness, fever and headache. The patient died often 11 days of illness. Death was due to massive bleeding and DIC thought to be due to pancytopenia secondary to massive blood element phagocytosis. So, we reported one case which considered compatible for HMR, with a few elementary reviewed literatures.

호흡기 관련 아데노바이러스의 진단 및 형 분석을 위한 Single tube multiplex polymerase chain reaction의 개발

나병국,김지희,신구철,이주연,이진수,강춘,김우주 국립보건연구원 2000 국립보건원보 Vol.37 No.-

Lithium ion capacitors fabricated with polyethylene oxide-functionalized polysilsesquioxane hybrid ionogel electrolytes

Na, W.,Lee, A.S.,Lee, J.H.,Hwang, S.S.,Hong, S.M.,Kim, E.,Koo, C.M. Pergamon Press 2016 ELECTROCHIMICA ACTA Vol.188 No.-

We present a new polyethylene glycol-functionalized polysilsesquioxane, a ladder-like structured poly(poly(ethylene oxide)-co-methacryloxypropyl)silsesquioxane) (PEO-SQ) hybrid-type polymer gelator to fabricate hybrid ionogel electrolytes for lithium ion capacitors. The organic-inorganic hybrid polymer gelator contains not only an inorganic ladder-like polysilsesquioxane backbone for the thermal and electrochemical stability, but also dual organic pendant groups consisting of free-dangling PEO chains and unsaturated methacryloxypropyl groups for enhanced lithium ion dissociation and thermal curing function, respectively. The successful crosslinking of 1M LiTFSI in N-butyl-N-methylpyrrolidinium bis(trifluoromethylsulfonyl)imide (BMPTFSI) ionic liquid with only a small amount of PEO-SQ hybrid crosslinker (5wt%) gave non-leaking ionogels with high thermal stability (@?400<SUP>o</SUP>C), large ionic conductivities, and excellent lithium ion capacitor C-rate performance and cyclability, comparable to those of neat ionic liquid electrolyte and superior over conventional organic ionogels fabricated with organic crosslinkers without PEO-moeities.

^19F(p, αγ)^16O 반응의 α-γ 각상관

박상태,김기동,이대원,정운혁,노태익,정성채,이종환,나상균 동의대학교 기초과학연구소 1994 基礎科學硏究論文集 Vol.4 No.1

The α-γ angular correlations in the ^19F(p, αγ)^16O reaction have been measured using a 500 keV VDG accelerator at E_p=340 keV and a CaF₂ taget prepared by evaporation in vaccum. The measurement of the α-γ angular correlation between the α-rays from the decay of ^20Ne into ^16O and the γ-rays from ^16O has been performed using a Si surface barrier detector and a NaI(TI) detector to determine the spin of the 13.196 MeV level in ^20Ne, and those of the 6.14 MeV and the ground level in ^16O, together with the orbital angular momentum of the emitted α's. The α-γ angular correlations in the ^19F(p, αγ)^16O reaction have been also calculated for the assumed spins of the 13.196 MeV level in ^20Ne, and those of the 6.14 MeV and ground level in ^16O. The spins of the levels under condsideration have been determined by comparison of the calculated values with experimental value. The result makes a good agreement to Arnold's data.

연속파 레이저 스페클 寫眞法과 畵像處理에 의한 面內變位 測定에 관한 硏究

김충원,정낙규,나기대,김경석,김택현 한국비파괴검사학회 1990 한국비파괴검사학회지 Vol.10 No.1

This paper presents experimental results which explore the capability of a CW laser speckle photography for the measurement of in -plane displacement at high temperature. The serious restrictions on the application of the method seem to be the ambient air turbulence and the change of surface texture caused by the oxidation, as they tend to decorrelate the double exposured speckle patterns. In order to assess only the effect of air turbulence, a ceramics-coated stainless steel plate is heated in air and Ar-laser specklegrams are made with combination of temperature and lateral translation displacement. The slight reduction in visibility of Young's fringes i observed at 1000˚C. The analyses of Young's fringes are carried out by a image processing system using a TV-camera and computers, and the result agrees well with the micrometer reading. Futhermore, uncoated stainless steel and Hastelloy X plates are tested and the effect of oxidation is also evaluated. The experimental results demonstrate that a CW laser speckle photography is applicable at temperatures up to 1000˚C.

Beta-Amyloid Oligomers Activate Apoptotic BAK Pore for Cytochrome c Release

Kim, J.,Yang, Y.,Song, S.,Na, J.H.,Oh, K.,Jeong, C.,Yu, Y.,Shin, Y.K. Biophysical Society ; Published for the Biophysica 2014 Biophysical journal Vol.107 No.7

In Alzheimer's disease, cytochrome c-dependent apoptosis is a crucial pathway in neuronal cell death. Although beta-amyloid (Aβ) oligomers are known to be the neurotoxins responsible for neuronal cell death, the underlying mechanisms remain largely elusive. Here, we report that the oligomeric form of synthetic Aβ of 42 amino acids elicits death of HT-22 cells. But, when expression of a bcl-2 family protein BAK is suppressed by siRNA, Aβ oligomer-induced cell death was reduced. Furthermore, significant reduction of cytochrome c release was observed with mitochondria isolated from BAK siRNA-treated HT-22 cells. Our in vitro experiments demonstrate that Aβ oligomers bind to BAK on the membrane and induce apoptotic BAK pores and cytochrome c release. Thus, the results suggest that Aβ oligomers function as apoptotic ligands and hijack the intrinsic apoptotic pathway to cause unintended neuronal cell death.

A new Schiff-based chemosensor for chromogenic sensing of Cu2+, Co2+ and S2- in aqueous solution: experimental and theoretical studies

Min, C.,Na, S.,Shin, J.,Kim, J.,Jo, T.,Kim, C. CNRS 2017 NEW JOURNAL OF CHEMISTRY Vol.41 No.10

<P>A new Schiff-based multifunctional colorimetric chemosensor 1 was developed for the detection of various analytes (Cu2+, Co2+ and S2-). Sensor 1 could simply monitor Cu2+ and Co2+ via the naked eye in an aqueous environment. The quite low detection limits were found to be 0.19 mu M for Cu2+ and 0.18 mu M for Co2+, which were much lower than the values recommended by the World Health Organization (WHO) for Cu2+ (31.5 mu M) and Environmental Protection Agency (EPA) for Co2+ (17 mu M). Importantly, 1 showed high preferential selectivity for Cu2+ and Co2+ over competitive metal ions. For practical applications, the sensing abilities of 1 for detecting Cu2+ and Co2+ were examined in real water samples. In addition, 1 exhibited high selectivity for S2- even in the presence of other anions without any interference. Moreover, the sensing mechanisms of 1 toward Cu2+, Co2+ and S2- were explained by theoretical calculations.</P>