결핵균 30-kDa와 32-kDa 단백항원의 분리정제와 마우스 비장세포 증식에 미치는 영향

백태현,오명주,김화중,조은경,박정규,최대경 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.1

Identification and characterization of individual components of M.tuberculosis have long been a focus of research on tuberculosis. The 30-kDa and 32-kDa antigens are major constituents of M bovis BCG and M. tuberculosis culture fluids. Because 30-kDa and 32-kDa antigens are partially identical, these antigens are difficult to purify in large amounts by biochemical techniques. This study was performed to purify the 30-kDa and 32-kDa antigens complexes primarily purified by 50% ammonium sulfate precipitation, hydroxylapatite chromatography and Sephadex G-75 gel filtration. And then further purification for separation of the two antigens was accomplished on preparative isoelectric focusing. Recovery of 30-kDa and 32-kDa antigens during above the purification procedures were 28% and 14%, respectively, and 147.0 and 59.8-fold purification were showed, respectively. On silver stained SDS-PAGE gels, the purified 32-kDa antigen gave a single band at 32-kDa molecule, while 30-kDa antigen gave one major band at 30-kDa molecule and faint additional band at 32-kDa. The pI of 30kDa-and 32-kDa antigens were 4.3 and 4.6, respectively. The partial identity between these two antigens was observed through the same pattern of reactivity of antigens in the ELISA and precipitation pattern of immunodiffusion. We also examined the immunological activities of both purified antigens by splenocyte proliferation of BCG-infected mice. Proliferative response to the 30-kDa and 32-kDa antigens did not show significant difference. However, macrophage-like cells existed in spleen cells from BCG-infected mice were involved in the suppression of the proliferative response to mycobacterial antigen. These results suggest that the 30-kDa and 32-kDa antigens could be effectively purified by the IEF and reactions of partial identity between the two antigens were found. However, 30-kDa antigen was the more immunogenic antigen than 32-kilodalton.

간호대학생의 감사성향이 대학생활 스트레스에 미치는 영향

조명주(Jo, Myoung-Ju) 계명대학교 간호과학연구소 2017 계명간호과학 Vol.21 No.1

Purpose: This study was conducted to investigate the effect of gratitude on life stress in nursing students. Methods: A total of 106 nursing students participated in the study. Data were analyzed using frequencies, t-test, ANOVA, pearson correlation coefficient, and hierarchical regression analysis with SPSS WIN 18.0 program. Results: The mean scores for gratitude and life stress were above-average level and below-average level respectively. There were significant mean differences in life stress according to sex and perceived physical health status. Life stress negatively correlated with gratitude. Gratitude was a significant factor of life stress explaining 16.6% of the variances. Conclusion: To decrease life stress among nursing students, nursing educators need to develop educational intervention programs to enhance gratitude.

간호대학생의 행복에 영향을 미치는 요인

조명주(Myoung-Ju Jo) 한국간호교육학회 2016 한국간호교육학회지 Vol.22 No.2

Purpose: This study explored factors that influence happiness in nursing students. Methods: The participants were 182 nursing students at a university in B city. Data were collected with a structured questionnaire. Data were analyzed using descriptive analysis, t-test, ANOVA, Pearson correlation coefficients, and multiple regression with the SPSS Win 22.0. Results: Happiness in nursing students correlated significantly with resilience (r=.79, p<.001) and self-differentiation (r=.38, p<.001). Resilience, subjective health status, and selection motivation to study nursing explained 69.1% of the variance in happiness. Conclusion: The study shows resilience, subjective health status, and selection motivation to study nursing are important factors for happiness in nursing students. These findings can be utilized to develop strategies for increasing nursing students’ happiness.

간호대학생과 산과 및 아동 간호사의 미혼모에 대한 인식과 성태도

조명주(Myoung-Ju Jo) 한국산학기술학회 2021 한국산학기술학회논문지 Vol.22 No.5

본 연구는 간호대학생과 산과 및 아동 간호사의 미혼모에 대한 인식과 성태도를 확인하고 대상자의 일반적 특성에 따른 차이를 파악하기 위해 실시하였다. 연구대상자는 B광역시와 U광역시에 간호대학에 재학 중인 3·4학년 학생 94명과 산과 및 소아청소년과에서 근무하는 8개 병원 간호사 101명이다. 자료수집은 2018년 9월부터 2019년 1월까지 실시하였다. 수집된 자료는 빈도와 백분율, 평균과 표준편차, independent t-test, one-way ANOVA로 분석하였다. 간호대학생과 간호사의 미혼모에 대한 인식과 성태도의 정도를 확인한 결과, 간호대학생의 미혼모에 대한 인식과 성태도의 정도 모두 유의한 차이가 있었다. 간호대학생의 일반적 특성에 따른 미혼에 대한 인식의 차이는 학년, 나이, 형제관계에서 유의한 차이가 있었고, 간호사는 나이, 직급, 총 근무기간, 현부서 근무기간, 결혼 여부, 자녀 유무에서 유의한 차이가 있었다. 간호대학생의 일반적 특성에 따른 성태도의 차이는 자신의 주변에서 미혼모 접촉 경험에서 유의한 차이가 있었고, 간호사는 주변에서 미혼모 접촉 경험, 병원 규모, 결혼 여부에서 유의한 차이가 있었다. 본 연구는 간호대학생과 간호사의 미혼모에 대한 인식과 성태도를 규명하고 대상자의 특성에 따른 차이를 확인하여, 향후 간호대학생과 간호사의 미혼모에 대한 인식과 성태도와 관련된 연구와 교육의 기초자료로 활용하고자 한다. This study was undertaken to identify the perception and sexual attitude of nursing students and obstetrics and pediatric nurses towards unwed mothers, and to identify differences in the general characteristics. Data were collected as a self-reported questionnaire survey from September, 2018 to January, 2019. Subjects included 94 nursing students enrolled at 2 nursing colleges, and 101 nurses of 8 hospitals located in the B & C Metropolitan. The data collected was analyzed by descriptive statistics, frequencies, percentages, means and standard deviations, independent t-test, and one-way ANOVA. Comparing results obtained from nursing students and nurses revealed a statistically significant difference in their overall perception of an unwed mother and sexual attitude. The perception of an unwed mother showed significant differences considering the grade, age and sibling status of nursing students, whereas nurses revealed significantly different perceptions according to their age, occupational position, clinical career, career of current work department, marital status, and children status. Sexual attitude was significantly different for nursing students according to their experience contact with unwed mother, whereas perception of nurses differed significantly for sexual attitude according to experience contact with unwed mother, types of hospital, and marital status. We propose that data obtained from this research can be used as basic data for research and education related to the perception of unwed mothers and sexual attitude of nursing students and nurses.

입원정신질환자의 자살사고에 영향을 미치는 요인

조명주(Myoung-Ju Jo),전원희(Won-Hee Jun) 한국콘텐츠학회 2014 한국콘텐츠학회논문지 Vol.14 No.11

본 연구는 입원정신질환자의 자살사고에 영향을 미치는 요인을 규명하는 서술적 조사연구이다. 연구대상자는 C 대학병원 정신과 병동에 입원한 정신질환자 186명이었다. 구조화된 자가 보고식 설문지를 이용하여 자료를 수집하였으며, 자료 분석은 SPSS WIN 프로그램을 사용하여 빈도와 백분율, 평균과 표준편차, t-test, ANOVA, Pearson correlation coefficient, hierarchical multiple regression analysis를 실시하였다. 연구결과, 자살사고는 결혼상태, 진단명, 자살시도 과거력에 따라 유의한 차이가 있었고 우울, 자아존중감, 영성과 유의한 상관관계가 있었다. 일반적 특성 중 자살사고에 유의한 차이를 보였던 결혼상태, 진단명, 자살시도 과거력을 통제한 상태에서 위계적 회귀분석을 실시한 결과, 자살사고에 영향을 미치는 요인으로는 우울과 영성이 유의한 것으로 나타났으며 이들 변수의 자살사고에 대한 설명력은 52.4%이었다. 결론적으로, 입원정신질환자들의 자살사고를 감소시키기 위해서 이들의 우울을 감소시키고 영성을 증진하는 중재프로그램을 개발하여 적용할 필요가 있다. The purpose of this study was to investigate factors affecting suicidal ideation in psychiatric inpatients. A total of 186 psychiatric inpatients participated in the study. Data were analyzed by frequencies, t-test, ANOVA, Pearson correlation coefficient, and hierarchical multiple regression with SPSS WIN 18.0. Suicidal ideation showed significant differences according to marital status, diagnosis, and previous suicide attempt history. There were significant correlations among depression, self-esteem, spirituality and suicidal ideation. Depression and spirituality were identified as predictors affecting suicidal ideation. The regression model explained 52.4% of suicidal ideation. As a result, intervention program for decreasing depression and improving spirituality is essential to decrease suicidal ideation in psychiatric inpatients.

간호대학생의 희망, 감사성향이 영성에 미치는 영향

조명주(Jo, Myoung-Ju),전원희(Jun, Won-Hee) 한국간호교육학회 2014 한국간호교육학회지 Vol.20 No.4

Purpose: This study investigates the factors affecting spirituality in nursing students. Method: A total of 247 nursing students participated in the study. Data were analyzed by frequencies, t-tests, ANOVA, Pearson correlation coefficients and multiple stepwise regression via SPSS WIN 18.0. Results: The mean scores for hope, gratitude, and spirituality were above-average. However, there were significant mean differences in spirituality according to religion, religious activity, voluntary service, satisfaction with the nursing field, and motivation for entering a college of nursing. Spirituality positively correlated with hope and gratitude. Significant predictors of spirituality included hope, gratitude, voluntary service and religious activity. The regression model explained 51.3% of perceived spirituality. Conclusion: To improve spirituality among nursing students, nursing educators should develop educational intervention programs to promote hope and gratitude, and support participation in voluntary service and religious activity.

성찰일지를 이용한 임종간호교육이 간호대학생의 영성, 죽음에 대한 태도, 임종간호태도에 미치는 효과

조명주(Jo myoung-ju),전원희(Jun won-hee) 한국콘텐츠학회 2015 한국콘텐츠학회 종합학술대회 논문집 Vol.2015 No.5

학령전기 아동을 둔 어머니의 자녀 성교육에 대한 부모효능감 영향 요인

조명주(Jo, Myoung-Ju),임견주(Lim, Kyoun-Joo),최은정(Choi, Eun Joung) 한국보건간호학회 2018 韓國保健看護學會誌 Vol.32 No.2

Glutathione (GSH) Response as a Metabolic Biomarker to Benzo(α)pyrene and Aroclor 1254 Exposure in the Pacific Oyster Crassostrea gigas

Jo, Qtae,Choy, Eun-Jung,Lee, Yong-Hwa,Ko, Sung-Jung,Song, Young-Chae,Kim, Myoung-Jin,Hwang, Eung-Ju The Korean Society of Fisheries and Aquatic Scienc 2006 Fisheries and Aquatic Sciences Vol.9 No.4

We measured activities of the ubiquitous tripeptide non-protein thiol (L-${\gamma}$-glutamyl-L-cysteinyl-glycine), glutathione (GSH), which is believed to playa fundamental role in detoxifying xenobiotics in biological systems, as a metabolic biomarker for benzo(${\alpha}$)pyrene and Aroclor 1254 exposure in the Pacific oyster Crassostrea gigas. Reproductive oysters were exposed to the pollutants for 50 days by the algal vectoring method in which the oysters were fed with concentrated standard algal foods grown in culture media containing Aroclor 1254 (0, 5, 50, 500 ng/g) or benzo(${\alpha}$)pyrene (0, 10, 100, 1,000 ng/g). Both pollutants induced maternal GSH activities in 10 days in a dosage-dependent manner (p<0.05), although Aroclor 1254 was stronger. The pollutant-driven GSH elevation persisted for 20 to 30 days depending on the pollutants and concentrations. Thereafter, a drastic decline in the GSH activity was observed due to metabolic failure, after which the oyster GSH remained at low levels throughout the remainder of the experiment. The pollutant exposures influenced maternal reproductive output in terms of fertilization, hatching, and morphology. These results imply that changes in activity of the GST-catalyzing molecule can be used as an oyster biomarker for Aroclor 1254 and benzo(${\alpha}$)pyrene exposure.

Anti-Cancer Effects of Dendropanax morbiferus Organs Extracts in Huh-7 Cells

Ju-Sung Kim,Mi Kyung Cha,Jong Hwan Ra,Tae Kyung Hyun,Myoung Jo Kim 한국약용작물학회 2012 한국약용작물학회 학술대회논문집 Vol.2012 No.2