Motivating people to sustain healthy lifestyles using persuasive technology: A pilot study of Korean Americans with prediabetes and type 2 diabetes

Kim, Miyong T.,Kim, Kim Byeng,Nguyen, Tam H.,Ko, Jisook,Zabora, Jim,Jacobs, Elizabeth,Levine, David Elsevier 2019 Patient education and counseling Vol.102 No.4

<P><B>Abstract</B></P> <P><B>Objective</B></P> <P>To test the efficacy of a hybrid model of the self-help intervention program (hSHIP), which combines a mobile version of SHIP (mSHIP) and personal coaching, to address unique cultural and motivational factors for optimal self-management of type 2 diabetes and prediabetes among Korean Americans (KAs).</P> <P><B>Methods</B></P> <P>A single-group feasibility study design was used. The hSHIP utilizes texts and motivational counseling based on well-tested intervention content for KAs. To facilitate the dissemination of hSHIP, we developed a web application adopting the principles of persuasive technology to motivate behavior changes.</P> <P><B>Results</B></P> <P>Feasibility assessment found that hSHIP was well accepted by both participants and community health workers who delivered the intervention. An average of 1.3% A1C reduction (from 7.8% to 6.5%) was achieved by KAs with diabetes (n = 165), 51.5% of whom lowered their A1C below 6.5% in 6-months. No one with prediabetes (n = 50) progressed to diabetes. Other clinical outcomes (e.g., weight, depression, and blood pressure) also improved significantly; 41.2% were able to reduce or discontinue antidiabetic drugs.</P> <P><B>Conclusion</B></P> <P>The feasibility and initial efficacy of hSHIP were demonstrated.</P> <P><B>Practice implication</B></P> <P>This hybrid diabetes self-management model is a viable tool for traditionally underserved groups with diabetes or prediabetes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We tested feasibility of a hybrid diabetes care model in an ethnic minority group. </LI> <LI> Our model combined a digital touch of mHealth and a human touch of CHWs. </LI> <LI> About a half (51.5%) lowered their hemoglobin A1C below 6.5% in 6 months. </LI> <LI> Many (41.2%) stopped/reduced antidiabetic drugs but still managed their diabetes. </LI> <LI> Weight, depression and blood pressure were also significantly improved. </LI> </UL> </P>

Exosomal PD-L1 promotes tumor growth through immune escape in non-small cell lung cancer

Dong Ha Kim,HyeongRyul Kim,Yun Jung Choi,Seon Ye Kim,Jung Eun Lee,Ki Jung Sung,Young Hoon Sung,Chan-Gi Pack,Min Kyo Jung,Buhm Han,Kunhee Kim,Woo Sung Kim,Soo Jeong Nam,Chang Min Choi,Miyong Yun,Jae Ch 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Programmed cell death protein-1/programmed cell death ligand-1 (PD-1/PD-L1) pathway blockade is a promising new cancer therapy. Although PD-1/PD-L1 treatment has yielded clinical benefits in several types of cancer, further studies are required to clarify predictive biomarkers for drug efficacy and to understand the fundamental mechanism of PD-1/PD-L1 interaction between host and tumor cells. Here, we show that exosomes derived from lung cancer cells express PD-L1 and play a role in immune escape by reducing T-cell activity and promoting tumor growth. The abundance of PD-L1 on exosomes represented the quantity of PD-L1 expression on cell surfaces. Exosomes containing PD-L1 inhibited interferon-gamma (IFN-γ) secretion by Jurkat T cells. IFN-γ secretion was restored by PD-L1 knockout or masking on the exosomes. Both forced expression of PD-L1 on cells without PD-L1 and treatment with exosomes containing PD-L1 enhanced tumor growth in vivo. PD-L1 was present on exosomes isolated from the plasma of patients with non-small cell lung cancer, and its abundance in exosomes was correlated with PD-L1 positivity in tumor tissues. Exosomes can impair immune functions by reducing cytokine production and inducing apoptosis in CD8+ T cells. Our findings indicate that tumor-derived exosomes expressing PD-L1 may be an important mediator of tumor immune escape.

Efficacy of nano‐particulated, water‐soluble erlotinib against intracranial metastases of EGFR ‐mutant lung cancer

Kim, Dong Ha,Choi, Yun Jung,Sung, Ki Jung,Yoo, Seon‐,A,Sung, Young Hoon,Kim, Jeong Kon,Choi, Chang‐,Min,Yun, Miyong,Lee, Eun Yong,Jin, Yong Suk,Cook, Seungho,Rho, Jin Kyung,Lee, Jae Cheol John Wiley and Sons Inc. 2018 Molecular Oncology Vol.12 No.12

<P>Central nervous system (CNS) metastasis is one of the serious complications of epidermal growth factor receptor (EGFR)‐mutant lung cancer, which arises due to poor penetration of the brain–blood barrier by EGFR‐tyrosine kinase inhibitors (EGFR‐TKIs). Although osimertinib, a third‐generation EGFR‐TKI, has efficacy against CNS metastases, further treatment modalities are still needed as some of these lesions do not respond to osimertinib, or undergo progression after an initial response to this drug if radiotherapy has already been conducted. Here, we investigated the efficacy of water‐soluble erlotinib (NUFS‐sErt) against these metastases. This agent was synthesized using a nano‐particulation platform technology utilizing fat and supercritical fluid (NUFS™) to resolve the low solubility problem that typically prevents the creation of injectable forms of EGFR‐TKIs. The average NUFS‐sErt particle size was 236.4 nm, and it showed time‐dependent dissolution in culture media. The effects of NUFS‐sErt were similar to those of conventional erlotinib in terms of inhibiting the proliferation of EGFR‐mutant lung cancer cells and suppressing EGFR signaling. In an intraperitoneal xenograft model of HCC827 cells, intraperitoneal administration of NUFS‐sErt produced a dose‐dependent inhibition of tumor growth and enhanced survival rate. Notably, the injection of NUFS‐sErt into the brain ventricle caused significant tumor growth inhibition in an intracranial xenograft model. Hence, our current findings indicate that NUFS‐sErt is a novel, water‐soluble form of erlotinib that can be administered using intraventricular or intrathecal injections. The target cases would be patients with a progressive CNS metastasis and no other therapeutic options. This drug could also be given intravenously to patients with swallowing difficulties or an inability to ingest due to a medical condition.</P>

Preconceptional use of folic acid and knowledge about folic acid among low-income pregnant women in Korea

Jihyun Kim,Miyong Yon,Cho-il Kim,Yoonna Lee,Gui-Im Moon,Jinhwan Hong,Taisun Hyun 대한지역사회영양학회 2017 Nutrition Research and Practice Vol.11 No.3

BACKGROUND/OBJECTIVES: Folic acid supplementation before pregnancy is known to significantly reduce the risk of having a baby with neural tube defects (NTDs). Therefore, it is important for women to be aware of the effects of folic acid supplementation before pregnancy. The purpose of this study was to investigate the awareness and preconceptional use of folic acid and to assess the current knowledge about folic acid among low-income pregnant women in Korea. SUBJECTS/METHODS: A questionnaire survey was conducted in 2012. Five hundred pregnant women were selected from the waiting list for the Nutriplus program implemented in public health centers using a multistage clustered probability sampling design. Data from 439 women were analyzed after excluding ones with incomplete answers. RESULTS: Among women who responded to the questionnaire, 65.6% had heard of folic acid before pregnancy, and 26.4% reported on the preconceptional use of folic acid. Women with a university degree or higher education were more likely to be aware of folic acid and to take folic acid in the preconception period. In a multivariate logistic regression, when age, education level, household income, employment status, gravidity, parity, and folic acid awareness were included in the model, folic acid awareness was a strong predictor of preconceptional folic acid use. As of interview, 85.4% and 77.7% of women were aware of the NTD-preventive role of folic acid and the appropriate time to take folic acid, respectively. The main sources of information on folic acid were healthcare professionals (41.2%), friends and family members (31.2%), and the media (26.5%). CONCLUSIONS: Our results suggest that public health strategies are needed to increase the preconceptional use of folic acid among Korean women.

p31comet Induces cellular senescence through p21 accumulation and Mad2 disruption.

Yun, Miyong,Han, Young-Hoon,Yoon, Sun Hee,Kim, Hee Young,Kim, Bu-Yeo,Ju, Yeun-Jin,Kang, Chang-Mo,Jang, Su Hwa,Chung, Hee-Yong,Lee, Su-Jae,Cho, Myung-Haing,Yoon, Gyesoon,Park, Gil Hong,Kim, Sang Hoon,L American Association for Cancer Research 2009 Molecular Cancer Research Vol.7 No.3

<P>Functional suppression of spindle checkpoint protein activity results in apoptotic cell death arising from mitotic failure, including defective spindle formation, chromosome missegregation, and premature mitotic exit. The recently identified p31(comet) protein acts as a spindle checkpoint silencer via communication with the transient Mad2 complex. In the present study, we found that p31(comet) overexpression led to two distinct phenotypic changes, cellular apoptosis and senescence. Because of a paucity of direct molecular link of spindle checkpoint to cellular senescence, however, the present report focuses on the relationship between abnormal spindle checkpoint formation and p31(comet)-induced senescence by using susceptible tumor cell lines. p31(comet)-induced senescence was accompanied by mitotic catastrophe with massive nuclear and chromosomal abnormalities. The progression of the senescence was completely inhibited by the depletion of p21(Waf1/Cip1) and partly inhibited by the depletion of the tumor suppressor protein p53. Notably, p21(Waf1/Cip1) depletion caused a dramatic phenotypic conversion of p31(comet)-induced senescence into cell death through mitotic catastrophe, indicating that p21(Waf1/Cip1) is a major mediator of p31(comet)-induced cellular senescence. In contrast to wild-type p31(comet), overexpression of a p31 mutant lacking the Mad2 binding region did not cause senescence. Moreover, depletion of Mad2 by small interfering RNA induced senescence. Here, we show that p31(comet) induces tumor cell senescence by mediating p21(Waf1/Cip1) accumulation and Mad2 disruption and that these effects are dependent on a direct interaction of p31(comet) with Mad2. Our results could be used to control tumor growth.</P>

항공기용 10㎸A 교류 발전기의 설계, 개발 및 시험평가

김미용(Miyong Kim),김영민(Youngmin Kim),박장홍(Janghong Park) 항공우주시스템공학회 2019 항공우주시스템공학회 학술대회 발표집 Vol.2019 No.11

This study describes the design, prototyping, and test evaluation of a 10kVA AC generator for an aircraft. The design was carried out considering the physical requirements, functional requirements, and environmental requirements of the aircraft system. The AC generator performs design verification through electromagnetic design and analysis, structural design and analysis, and thermal / flow analysis, and performs design, development, manufacturing and test evaluation according to the AS9100 quality system. Environmental testing was performed in accordance with the requirements of the RTCA DO-160G, and this document describes the overall process and results from design to test evaluation.

애기장대(Arabidopsis thaliana)의 잎과 줄기 절편체로부터 기관분화를 통한 식물체 재분화

Myoung Duck KIM,Miyong Chang,Joon Chul KIM 강원대학교 기초과학연구소 2000 기초과학연구 Vol.11 No.-

Shoot induction from leaf and stern explants of Arabidopsis tfr1liana was obtained when leaf explants were cultured on MS medium containing 0.15 mg/L lAA and 5 mg/L 2-jp after liquid preculture on MS medium containing 5 mg/L lAA and 0.5 mg/L 2,4- D for 7 days and also, when stem explants were cultured on MS medium containing 0.15 mg/L lAA and 5 mg/L 2-jp after liquid preculture on CP medium containing 0.5 mg/L 2,4-D and 0.1 mg/L BAP for 7 days. Callus induction from stern explants was obtained on CP medium containing 0.5 mg/L 2,4-D and 0.1 mg/L BAP in the dark at 25 t: after four weeks of culture. Shoot forming calli selected from subcultured-calli were cultured on MS medium containing 0.05 mg/L lAA and 7 mg/L 2-jp under continous illumination for 4 weeks. Shoot regenerating frequency (explants and calli regenerating at least one shoot) was more than 55%. Regenerated shoots were rooted on MS medium without phytohomones and transplanted to potting soil. Histological observations of leaf and stern explants revealed that meristematic dome was originated from massive cells of epidermal and subepidermal cells and that meristemoid from the shoot forming calli developed to shoot primordia and shoot.

Regulation of Crosstalk between Epithelial to Mesenchymal Transition Molecules and MMP-9 Mediates the Antimetastatic Activity of Anethole in DU145 Prostate Cancer Cells

Ha, ByungChul,Ko, HyunSuk,Kim, BongLee,Sohn, Eun Jung,Jung, Ji Hoon,Kim, Ji Sung,Yoon, Jung Jae,Won, Gunho,Kim, Ji-Hyun,Jung, Deok-beom,Yun, Miyong,Shim, BumSang,Kim, Sung-Hoon American Chemical Society and American Society of 2014 Journal of natural products Vol.77 No.1

<P>The underlying antimetastatic mechanism of anethole (<B>1</B>) still remains unclear in association with the molecules of the epithelial to mesenchymal transition (EMT). Herein, the role of the EMT molecules was elucidated in terms of the antimetastatic activity of <B>1</B> using DU145 cells. Anethole significantly inhibited the adhesion of DU145 cells to vitronectin-coated plates, as well as migration in a wound-healing assay and invasion using a Boyden chamber. Also, anethole suppressed the expression of MMP-9 in DU145 cells by zymography, ELISA, and RT-PCR. Consistently, the silencing of MMP-9 enhanced the activity of <B>1</B> to upregulate the expression of E-cadherin and to attenuate the expression of Vimentin in DU145 cells. Compound <B>1</B> enhanced E-cadherin, which is an epithelial marker and attenuated the expression of Vimentin, Twist, and Snail as mesenchymal molecules at the mRNA level. Consistently, anethole upregulated E-cadherin and downregulated the expression of Vimentin, Twist and PI3K, and AKT at the protein level in DU145 cells. Conversely, the antimetastatic effects of <B>1</B> to inhibit invasion and the expression of MMP-9 and upregulate E-cadherin were reversed by the EMT inducer TGF-β in DU145 cells. Overall, the present findings suggest that anethole exerts antimetastatic activity via regulation of crosstalk between EMT molecules and MMP-9 on the basis of the in vitro data obtained.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2014/jnprdf.2014.77.issue-1/np4006376/production/images/medium/np-2013-006376_0006.gif'></P>

A derivative of epigallocatechin-3-gallate induces apoptosis via SHP-1-mediated suppression of BCR-ABL and STAT3 signalling in chronic myelogenous leukaemia : EGCG-MP effectively induces apoptosis in CML

Jung, Ji Hoon,Yun, Miyong,Choo, Eun-Jeong,Kim, Sun-Hee,Jeong, Myoung-Seok,Jung, Deok-Beom,Lee, Hyemin,Kim, Eun-Ok,Kato, Nobuo,Kim, Bonglee,Srivastava, Sanjay K,Kaihatsu, Kunihiro,Kim, Sung-Hoon Wiley (Blackwell Publishing) 2015 British journal of pharmacology Vol.172 No.14

Measures for a closer-to-real estimate of dietary exposure to total mercury and lead in total diet study for Koreans

Eunmi Koh,Hyehyung Shin,Miyong Yon,Ji Woon Nam,Yoonna Lee,Dohee Kim,Jeeyeon Lee,Meehye Kim,Sung-Kug Park,Hoon Choi,Cho-il Kim 한국영양학회 2012 Nutrition Research and Practice Vol.6 No.5

Previous Korean total diet studies (KTDSs) have estimated dietary exposure to toxic chemicals based on 110-120 representative foods selected from over 500 foods appeared in the Korea National Health & Nutrition Examination Surveys (KNHANES), which would result in a possible underestimation. In order to find measures for a closer-to-real estimate of dietary exposure to heavy metals, this study examined the feasibility of mapping foods to the representative foods in the KTDS by comparing estimates. In mapping, those foods not analyzed in the 2009 KTDS (443 out of 559 foods appeared in the 2007 KNHANES) were mapped to the 114 representative foods used in the 2009 KTDS based on the closeness in regards to biological systematics and morphological similarity. Dietary exposures to total mercury and lead were re-estimated using the content of total mercury and lead in 114 foods analyzed in the 2009 KTDS, food intake, and individual’s own body weight for respondents in the 2007 KNHANES instead of mean body weight of Koreans used in the 2009 KTDS. The re-estimates of exposure with mapping were approximately 50% higher than the original estimates reported in the 2009 KTDS. In addition, mapping enabled the comparison of percentile distribution of the exposure among populations of different age groups. In conclusion, estimates via mapping resulted in a more comprehensive estimation of dietary exposure to heavy metals present in foods that Koreans consume.