Time-degenerative Factors and the Risk of Hepatocellular Carcinoma after Antiviral Therapy among HCV Patients: A Model for Prioritization of Treatment

( Ming-lung Yu ),( Chung-feng Huang ),( Ming-lun Yeh ),( Jee-fu Huang ),( Chia-yen Dai ),( Wan-long Chuang ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1

Aims: Age and hepatic fibrosis are the factors that increase the risk of hepatocellular carcinoma (HCC) over time. We aimed to explore their impac at the initiation of antiviral therapy on HCC among chronic hepatitis C (CHC) patients. Methods: A total of 1281 biopsy-proven CHC patients receiving interferon- based therapy were followed for a mean period of 5.5 years. Results: The 5-year cumulative incidence of HCC did not differ between non-SVR and SVR patients who were <40 years old (7.7 % vs. 0.5%, P=0.1), but was significantly higher in non-SVR patients between 40 and 55 years old (18.0% vs. 1.3%, P<0.001) and >55 years old (15.1% vs. 7.9%, P=0.03). Compared with SVR, non-SVR was independently predictive of HCC in patients 40-55 years old (hazard ratio [HR]/95% confidence intervals [CI]: 10.92/3.78-31.56, P<0.001) and >55 years old (HR/CI: 1.96/1.06-3.63, P=0.03) but not in patients <40 years old (HR/CI: 2.76/0.41-18.84, P=0.3). The 5-year cumulative incidence of HCC did not differ between non-SVR and SVR patients whose fibrosis stage was F0-1 (4.6% vs. 1.9%, P=0.25) but was higher in non-SVR patients with F2-3 (21.4% vs. 4.3%, P<0.001) or F4 (33.5% vs. 8.4%, P=0.002). Compared with SVR, non-SVR was independently predictive of HCC in patients with F2-3 (HR/CI: 4.36 /2.10-9.03, P<0.001) and F4 (HR/CI: 3.84/1.59-9.30, P=0.03) but not in those with F0-1 (HR/CI: 1.53/ 0.49-4.74, P=0.47). Conclusions: Delayed HCV clearance for patients with CHC > 40 years old or with a fibrosis stage > 2 increases the risk of HCC over time.

Tight sportswear and physiological function-Effect on muscle strength and EMG activity-

Xiao Qun Dai,Yu Ping Li,Juan Juan Cai,A Ming Lu,Guo Dong Wang 복식문화학회 2013 服飾文化硏究 Vol.21 No.4

The Different Expression of Gene Profiles on Hepatocellular Carcinoma Cells with Different Intracellular Hepatitis C Viral Load

( Chia-yen Dai ),( Shu-chi Wang ),( Meng-hsuan Hsieh ),( Cheng-fu Yang ),( Ching-i Huang ),( Chung-feng Huang ),( Ming-lun Yeh ),( Jee-fu Huang ),( Wang-long Chung ),( Ming-lung Yu ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: The different hepatitis C virus (HCV) replication has been reported among individual hepatocytes in chronic HCV infection by identifying hepatocytes with different HCV RNA levels. We have previously established a fluorescence-activated cell sorting (FACS) protocol to study the effects of different intracellular viral loads in HCV-infected cells. The present study aimed to further study the gene expression on different hepatocellular carcinoma (HCC) cells with different HCV viral load. Methods: The JFH1-EYFP viral florescence intensity was used to sort the high and low viral load cells after 5 days infection in vitro which has been shown in our previous study that infected cells efficiently and accurately discriminated between high- and low-viral load cell populations. The next generation sequence-RNA sequence was used to clarify the mRNA and miRNA gene network between HCV-high and HCV-low infected cells of the HCC cell line. Venn diagram summarizing the probe sets that were differentially expressingbetween the Huh7.5.1 versus each differential viral load cell population and miRDB and miRTar databases were used to predict HVL and LVL/S2 unique miRNA target genes. Results: By analyzing the NGS dataset and miRNA microarray dataset, of the significant transcripts, three miRNA were unique for the LVL/S2 cells and nine miRNA unique for the HVL. Twenty-three miRNA were common for all 3 viral load groups. We verified them by q-PCR and data confirmed the array data expression level. We found that high viral loads were associated with cell inflammation- and cell death-associated pathway; and the low viral loads were associated many stress response- and cell adhesion molecular (CAMs)-related genes. Conclusions: With the established cell sorting protocol, we have demonstrated that different gene network between HCV-high and HCV-low infected cells in JFH1-EYFP infectious cells exists. Our results may provide a boarder gene regulation map between high and low viral load cell populations.

Could Tumor Size Be A Predictor for Papillary Thyroid Microcarcinoma: a Retrospective Cohort Study

Wang, Min,Wu, Wei-Dong,Chen, Gui-Ming,Chou, Sheng-Long,Dai, Xue-Ming,Xu, Jun-Ming,Peng, Zhi-Hai Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.18

Background: Central lymph node metastasis(CLNM) is common in papillary thyroid microcarcinoma (PTMC). The aim of this study was to define the pathohistologic risk grading based on surgical outcomes. Materials and Methods: Statistical analysis was performed to figure out the optimal cut-off values of size in preoperative ultrasound images for defining the risk of CLNM in papillary thyroid microcarcinoma. Receiver operating characteristic curves (ROC) studies were carried out to determine the cutoff value(s) for the predictor(s). All the patients were divided into two groups according to the above size and the clinic-pathological and immunohistochemical parameters were compared to determine the significance of findings. Results: The optimal cut-off value of tumor size to predict the risk of CLNM in papillary thyroid microcarcinoma was 0.575 cm (area under the curve 0.721) according to the ROC curves. Significant differences were observed on the multifocality, extrathyroidal extension and central lymph node metastasis between two groups which were divided according to the tumor size by the cutoff values. Patients in two groups showed different positive rate and intensity of Ki67. Conclusions: The size of PTMC in ultrasound images are helpful to predict the aggressiveness of the tumors, it could be an easy predictor for PTMC prognosis and assist us to choose treatment.

Pre-treatment Metabolic Tumor Volume and Total Lesion Glycolysis are Useful Prognostic Factors for Esophageal Squamous Cell Cancer Patients

Li, Yi-Min,Lin, Qin,Zhao, Long,Wang, Li-Chen,Sun, Long,Dai, Ming-Ming,Luo, Zuo-Ming,Zheng, Hua,Wu, Hua Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.3

Objectives: To study application of the maximum standardized uptake value (SUVmax), metabolic tumor volume (MTV) and total lesion glycolysis (TLG) with $^{18}F$-FDG PET/CT for predicting prognosis of esophageal squamous cell cancer (ESC) patients. Methods: Eighty-six patients with ESC staged from I to IV were prospectively enrolled. Cisplatin-based chemoradiotherapy (CCRT) or palliative chemoradiotherapy were the main treatment methods and none received surgery. $^{18}F$-FDG PET/CT scans were performed before the treatment. SUVmax, MTV, and TLG were measured for the primary esophageal lesion and regional lymph nodes. Receiver operating characteristic curves (ROCs) were generated to calculate the P value of the predictive ability and the optimal threshold. Results: MTV and TLG proved to be good indexes in the prediction of outcome for the ESC patients. An MTV value of 15.6 ml and a TLG value of 183.5 were optimal threshold to predict the overall survival (OS). The areas under the curve (AUC) for MTV and TLG were 0.74 and 0.70, respectively. Kaplan-Meier analysis showed an MTV less than 15.6 ml and a TLG less than 183.5 to indicate good media survival time (p value <0.05). In the stage III-IV patient group, MTV could better predict the OS (P < 0.001), with a sensitivity and specificity of 0.80 and 0.67, respectively. Conclusions: Pre-treatment MTV and TLG are useful prognostic factors in nonsurgical ESC.

Development of High Spectral Resolution Lidar System for Measuring Aerosol and Cloud

Ming Zhao,Chen-Bo Xie,Zhiqing Zhong,Bang-Xin Wang,Zhenzhu Wang,Pang-Da Dai,Zhen Shang,Min Tan,Dong Liu,Yingjian Wang 한국광학회 2015 Current Optics and Photonics Vol.19 No.6

A high spectral resolution lidar (HSRL) system based on injection-seeded Nd:YAG laser and iodineabsorption filter has been developed for the quantitative measurement of aerosol and cloud. The laserfrequency is stabilized at 80 MHz by a frequency locking system and the absorption line of iodine cellis selected at the 1111 line with 2 GHz width. The observations show that the HSRL can provide verticalprofiles of particle extinction coefficient, backscattering coefficient and lidar ratio for cloud and aerosolup to 12 km altitude, simultaneously. For the measured cases, the lidar ratios are 10~20 sr for cloud,28~37 sr for dust, and 58~70 sr for urban pollution aerosol. It reveals the potential of HSRL to distinguishthe type of aerosol and cloud. Time series measurements are given and demonstrate that the HSRL hasability to continuously observe the aerosol and cloud for day and night

Research on Several Multicast Routing Algorithms

Dai Yin-fei,Liu Ming-hui 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.3

This paper first describes the dynamic multicast routing and routing optimization standard, then puts forward the RPL protocol based energy efficient DODAG multicast routing algorithm. Through simulation experiments, we can compare and analyze the advantages and disadvantages of several kinds of dynamic multicast routing algorithm. And according to the simulation results obtained conclusion, EE - DODAG multicast in the process of each packet sent after more a small amount of forwarding nodes, and consume less energy in survival time.

Tight sportswear and physiological function - Effect on muscle strength and EMG activity -

Dai, Xiao-Qun,Li, Yu-Ping,Cai, Juan-Juan,Lu, A-Ming,Wang, Guo-Dong The Costume Culture Association 2013 服飾文化硏究 Vol.21 No.4

It has been reported that tight sportswear could have complicated influence on physiological function of human body. The purpose of this present study was to investigate the effect of wearing gradient compression tights (GCT) on muscle strength and EMG activity during repeated isokinetic muscle contractions. Four healthy male undergraduate students performed maximal voluntary isokinetic concentric muscle contractions on biomechanical test and training systems with GCT and loose pants as control (Cont) respectively. During each test, the peak torque of extensor and flexor contractions and the surface electromyography (sEMG) of the rectus femoris and medial gastrocnemius was recorded simultaneously, the peak torque was recorded as an indicator of muscle strength, and the average amplitude and mean power frequency of sEMG were calculated as indicators of EMG activity. The results showed that: the peak torque decreased gradually during continuous muscle contractions both when the Cont and GCT were worn, average sEMG and mean power frequency declined along with the repetitions of muscle contractions for both wearing conditions, and the change tendency was consistence with that of peak torque. There was no obvious difference between the peak torque recorded wearing the Cont or wearing GCT, but when GCT were worn, average sEMG was lower and mean power frequency was higher than the Cont condition. In 24 samples obtained from four subjects, 80% of results showed the same trend. So we could make a conclusion that wearing GCT had no obvious effect on the improvement of muscle strength, but it would affect the EMG activity positivly.

Magnetoelectric Transducer Based Rotation Energy Harvester

Ming Li,Yumei Wen,Ping Li,Jin Yang,Xianzhi Dai 한국자기학회 2010 한국자기학회 학술연구발표회 논문개요집 Vol.2010 No.12

Dynamic Performance Analysis of Capture and Separation Process for a Microsatellite Docking Device

DAI Ye,ZHANG Yuan,LI Wen-juan,TANG Wen-ming 보안공학연구지원센터 2015 International Journal of Future Generation Communi Vol.8 No.3

Aiming at the problem of short lifetime and high cost in the on-orbit Servicing technology, a new type of automatic autonomous probe-cone docking mechanism used for microsatellite docking is proposed which can realize on-orbit special services. The structure of the mini docking mechanism is provided according to the design indices, and a virtual prototype model of the mini-type universal docking system is established by using MATLAB software. The dynamics modeling of the docking process is presented based on the virtual work principle. The simplified dynamic analysis model during the capture process is established. The dynamic characteristic analysis of the catching process is completed to research the influence of the key parameters, and the virtual prototype model of the docking device is established through ADAMS for further verifying the proposed design. The analysis shows that this mechanism has the good capacity of capture and separation process and energy absorption to reduce the impact during the docking process. And the simulation results meet the design demands of mini-type universal autonomous docking mechanism.