WRKY71 Acts Antagonistically Against Salt-Delayed Flowering in Arabidopsis thaliana

Yu, Yanchong,Wang, Long,Chen, Jiacai,Liu, Zhenhua,Park, Chung-Mo,Xiang, Fengning Oxford University Press 2018 Plant & cell physiology Vol.59 No.2

한국과 중국 대학생의 상거래에 있어서의 소비윤리 의식 및 행동에 관한 비교 연구

김효정 ( Hyo Chung Kim ),왕해용 ( Hai Long Wang ),김하 ( Xia Jin ),이승남 ( Sheng Nan Li ) 한국윤리학회 2013 倫理硏究 Vol.90 No.1

This study examined comparison of perceptions and behaviors of ethics of consumption in business transactions among college students in Korea and China, and factors affecting them. The data were collected from students in Gyeongnam region, Korea and Qinyang region, China through a self-administered questionnaire. Frequency, Cronbach`s α, t test, Pearson`s correlation analysis, and multiple regression analysis were conducted by SPSS Windows V.19.0. For Korean respondents, the mean of ``passively benefiting at the expense of others`` was highest in both of the perceptions and behaviors of ethics of consumption in business transactions, while for Chinese respondents, the mean of ``no harm/no foul`` was highest. Additionally, Korean respondents showed more ethical perceptions for ``actively benefiting from illegal activity``, ``actively benefiting from questionable behavior``, and ``no harm/ho foul`` than Chinese respondents. On the other hands, Korean respondents showed less unethical behaviors for ``actively benefiting from illegal activity``, and ``actively benefiting from questionable behavior`` than Chinese respondents. According to the multiple regression analysis, age, gender, idealism, and nationality were significant factors affecting perceptions and behaviors of ethics of consumption in business transactions, respectively. On the basis of these results, education of ethics of consumption for college students is needed to have desirable values of consumption.

Selective control of multiple ferroelectric switching pathways using a trailing flexoelectric field

Park, Sung Min,Wang, Bo,Das, Saikat,Chae, Seung Chul,Chung, Jin-Seok,Yoon, Jong-Gul,Chen, Long-Qing,Yang, Sang Mo,Noh, Tae Won Nature Publishing Group UK 2018 Nature nanotechnology Vol.13 No.5

<P>Flexoelectricity is an electromechanical coupling between electrical polarization and a strain gradient(1) that enables mechanical manipulation of polarization without applying an electrical bias(2,3). Recently, flexoelectricity was directly demonstrated by mechanically switching the out-of-plane polarization of a uniaxial system with a scanning probe microscope tip(3,4). However, the successful application of flexoelectricity in low-symmetry multiaxial ferroelectrics and therefore active manipulation of multiple domains via flexoelectricity have not yet been achieved. Here, we demonstrate that the symmetry-breaking flexoelectricity offers a powerful route for the selective control of multiple domain switching pathways in multiaxial ferroelectric materials. Specifically, we use a trailing flexoelectric field that is created by the motion of a mechanically loaded scanning probe microscope tip. By controlling the SPM scan direction, we can deterministically select either stable 71 degrees ferroelastic switching or 180 degrees ferroelectric switching in a multiferroic magnetoelectric BiFeO3 thin film. Phase-field simulations reveal that the amplified in-plane trailing flexoelectric field is essential for this domain engineering. Moreover, we show that mechanically switched domains have a good retention property. This work opens a new avenue for the deterministic selection of nanoscale ferroelectric domains in low-symmetry materials for non-volatile magnetoelectric devices and multilevel data storage.</P>

The HCV-Associated Hepatocarcinogenesis in Intracellular Low Viral Load Cells

( Chia-yen Dai ),( Shu-chi Wang ),( Chung-feng Huang ),( Wang-long Chung ),( Ming-lung Yu ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: There are differential viral load distribution in HCV infected liver tissues. We conduct the present study aimed to dissect the different viral load cells to investigate the viral-host resistance on the HCV asssoicated hepatocarcinogenesis. Methods: The study was performed using a replicated in vitro HCV-fluorescence infection model to discuss HCV-high viral load (HVL) cells and the HCV-low viral load (LVL) cells by Flow sort system. The next generation RNA sequence and miRNA array were used to explore the gene profiles and miRNA expression on different HCV-viral load cell populations. Results: The ROS indicator shows that the ROS abundantly in low viral load cells. The RNA-Seq analysis showed that significant enrichment in Cancer (P=5.00E-02 - 2.76E-04; 40 molecules) and a network of the Cellular Movement, Immune Cell Trafficking, Inflammatory Response (Score: 18) by IPA analysis. Protein analysis results confirmed the GADD45A and iNOS overexpression in the LVL cells which verified the oxidative stress qPCR array data in LVL cells. We also found the up-regulated Src oncoprotein expression and down-regulated E-cadherin expression in LVL cells. The miRNA array showed that miR-194, miR-192/215 and miR-10a were preferentially expressed in low viral load cells. Conclusions: With our established cell sorting system, this study provided the gene network between viral and host cells resistance by different viral load cells. The findings show the activated oxidative stress related-gene expression in hepatocytes is associated with the HCV-infected epithelial-to-mesenchymal transition (EMT), providing an important link between HCV viral load and liver cancer. The miRNA-gene intergraded dada need further studies.

Effect of Active Synthetic 2-Substituted Quinazolinones on Anti-Platelet Aggregation and the Inhibition of Superoxide Anion Generation by Neutrophils

Chang, Fang-Rong,Wu, Chin-Chung,Hwang, Tsong-Long,Patnam, Ramesh,Kuo, Reen-Yen,Wang, Wei-Ya,Lan, Yu-Hsuan,Wu, Yang-Chang The Pharmaceutical Society of Korea 2003 Archives of Pharmacal Research Vol.26 No.7

Quinazolinones, 2-substituted and 3-substituted, mainly synthesized by microwave irradiation, were subjected to anti-platelet aggregation and inhibition of superoxide anion generation assays. Interestingly, 2-phenyl-4-quinazolinone (4) exhibited significant inhibitory activities toward platelet aggregation and neutrophil activation, and it might therefore serve as a prototype lead compound.

The Different Expression of Gene Profiles on Hepatocellular Carcinoma Cells with Different Intracellular Hepatitis C Viral Load

( Chia-yen Dai ),( Shu-chi Wang ),( Meng-hsuan Hsieh ),( Cheng-fu Yang ),( Ching-i Huang ),( Chung-feng Huang ),( Ming-lun Yeh ),( Jee-fu Huang ),( Wang-long Chung ),( Ming-lung Yu ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: The different hepatitis C virus (HCV) replication has been reported among individual hepatocytes in chronic HCV infection by identifying hepatocytes with different HCV RNA levels. We have previously established a fluorescence-activated cell sorting (FACS) protocol to study the effects of different intracellular viral loads in HCV-infected cells. The present study aimed to further study the gene expression on different hepatocellular carcinoma (HCC) cells with different HCV viral load. Methods: The JFH1-EYFP viral florescence intensity was used to sort the high and low viral load cells after 5 days infection in vitro which has been shown in our previous study that infected cells efficiently and accurately discriminated between high- and low-viral load cell populations. The next generation sequence-RNA sequence was used to clarify the mRNA and miRNA gene network between HCV-high and HCV-low infected cells of the HCC cell line. Venn diagram summarizing the probe sets that were differentially expressingbetween the Huh7.5.1 versus each differential viral load cell population and miRDB and miRTar databases were used to predict HVL and LVL/S2 unique miRNA target genes. Results: By analyzing the NGS dataset and miRNA microarray dataset, of the significant transcripts, three miRNA were unique for the LVL/S2 cells and nine miRNA unique for the HVL. Twenty-three miRNA were common for all 3 viral load groups. We verified them by q-PCR and data confirmed the array data expression level. We found that high viral loads were associated with cell inflammation- and cell death-associated pathway; and the low viral loads were associated many stress response- and cell adhesion molecular (CAMs)-related genes. Conclusions: With the established cell sorting protocol, we have demonstrated that different gene network between HCV-high and HCV-low infected cells in JFH1-EYFP infectious cells exists. Our results may provide a boarder gene regulation map between high and low viral load cell populations.