Radicicol Inhibits iNOS Expression in Cytokine-Stimulated Pancreatic Beta Cells

Cha Kyung Youn,Seon Joo Park,Mei Hong Li,Min Young Lee,Kun Yeong Lee,Man Jin Cha,Ok Hyeun Kim,Ho Jin You,In Youp Chang,Sang Pil Yoon,Young Jin Jeon 대한생리학회-대한약리학회 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.4

Here, we show that radicicol, a fungal antibiotic, resulted in marked inhibition of inducible nitric oxide synthase (iNOS) transcription by the pancreatic beta cell line MIN6N8a in response to cytokine mixture (CM: TNF-Ձ, IFN-Ճ, and IL-1Ղ). Treatment of MIN6N8a cells with radicicol inhibited CM-stimulated activation of NF-ՊB/Rel, which plays a critical role in iNOS transcription, in a dose-related manner. Nitrite production in the presence of PD98059, a specific inhibitor of the extracellular signal-regulated protein kinase-1 and 2 (ERK1/2) pathway, was dramatically diminished, suggesting that the ERK1/2 pathway is involved in CM-induced iNOS expression. In contrast, SB203580, a specific inhibitor of p38, had no effect on nitrite generation. Collectively, this series of experiments indicates that radicicol inhibits iNOS gene expression by blocking ERK1/2 signaling. Due to the critical role that NO release plays in mediating destruction of pancreatic beta cells, the inhibitory effects of radicicol on iNOS expression suggest that radicicol may represent a useful anti-diabetic activity.

Radicicol Inhibits iNOS Expression in Cytokine-Stimulated Pancreatic Beta Cells

Youn, Cha Kyung,Park, Seon Joo,Li, Mei Hong,Lee, Min Young,Lee, Kun Yeong,Cha, Man Jin,Kim, Ok Hyeun,You, Ho Jin,Chang, In Youp,Yoon, Sang Pil,Jeon, Young Jin The Korean Society of Pharmacology 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.4

Here, we show that radicicol, a fungal antibiotic, resulted in marked inhibition of inducible nitric oxide synthase (iNOS) transcription by the pancreatic beta cell line MIN6N8a in response to cytokine mixture (CM: TNF-${\alpha}$, IFN-${\gamma}$, and IL-$1{\beta}$). Treatment of MIN6N8a cells with radicicol inhibited CM-stimulated activation of NF-${\kappa}B$/Rel, which plays a critical role in iNOS transcription, in a dose-related manner. Nitrite production in the presence of PD98059, a specific inhibitor of the extracellular signal-regulated protein kinase-1 and 2 (ERK1/2) pathway, was dramatically diminished, suggesting that the ERK1/2 pathway is involved in CM-induced iNOS expression. In contrast, SB203580, a specific inhibitor of p38, had no effect on nitrite generation. Collectively, this series of experiments indicates that radicicol inhibits iNOS gene expression by blocking ERK1/2 signaling. Due to the critical role that NO release plays in mediating destruction of pancreatic beta cells, the inhibitory effects of radicicol on iNOS expression suggest that radicicol may represent a useful anti-diabetic activity.

한천분해효소를 생산하는 해양세균 Thalassomonas sp. SL-5의 분리 및 특성

이동근,김남영,장민경,이옥희,이상현,Lee, Dong-Geun,Kim, Nam-Young,Jang, Min-Kyung,Lee, Ok-Hee,Lee, Sang-Hyeon 한국생명과학회 2007 생명과학회지 Vol.17 No.1

A novel agar-degrading bacterium SL-5 was isolated from seashore of Homigot at Kyung-Buk province, and cultured in marine broth 2216 media. The bacterium SL-5 was identified as Thalassomonas genus by 16S rDNA sequencing with 96% identity. Growth rate was faster at $27^{\circ}C$ than at $37^{\circ}C$ and agarase was produced as growth-related. The optimum pH of the enzyme activity was 7.0 and the optimum temperature for the reaction was $40^{\circ}C$. Although the enzyme had no thermostability, the enzyme activity was remained over 80% at $60^{\circ}C$. The enzyme hydrolyzed neoagarohexaose to yield neoagarobiose as the main product, indicating that the enzyme is $\beta-agarase$. Thus, the enzyme would be useful for the industrial production of neoagarobiose. 포항 호미곶 해수에서 한천분해활성을 보이는 해양성 세균 SL-5을 분리하였으며 16S rDNA 염기서열분석으로 해양기원의 Thalassomonas 속과 가장 유사한 균주임을 확인하였다. SL-5 균주가 생성하는 한천분해효소(agarase)는 성장의존성인 것으로 확인되었고 효소활성을 위한 최적 pH는 pH 7.0(20 mM sodium phosphate 완충용액)이고 최적 온도는 $40^{\circ}C$로 나타났다. 한천분해효소는 $80^{\circ}C$까지 65%의 효소활성을 보이는 호열성 효소이지만, 내열성은 그리 높지 않았다. 한천 분해효소의 분해산물에 대한 TLC 분석결과, 본 효소가 $\beta-agarase$라는 사실을 확인할 수 있었다. 분리한 Thalassomonas sp. SL-5 균주가 생산하는 한천분해효소를 이용하여 한천으로부터 다양한 기능성 한천올리고당을 생산하는데 있어 유용하게 사용될 것으로 기대된다.

Monitoring of particulate hazardous air pollutants and affecting factors in the largest industrial area in South Korea: The Sihwa-Banwol complex

Kyung-Min Baek,Young-Kyo Seo,Jun-Young Kim,Sung-Ok Baek 대한환경공학회 2020 Environmental Engineering Research Vol.25 No.6

Ambient air monitoring of particulate hazardous air pollutants was performed from 2005 to 2007 in the Sihwa-Banwol industrial complexes, which is the largest industrial area in Korea. The occurrence of polycyclic aromatic hydrocarbons (PAHs) and heavy metals, their spatial and seasonal distributions, and the factors affecting the variations in concentrations were investigated. The annual average concentration of benzo[a]pyrene was 1.27 ng/m³, with a maximum of 10.41 ng/m³. The PAH levels between the industrial and residential sites did not differ significantly, although heavy metals strongly related to industrial activities showed a clear variation between the two groups. Thus, industrial activities were not the only sources of PAHs; they also included automobile emissions and residential heating fuels. Coal-fired power plants outside the city and transboundary contributions from China and North Korea also affected the PAH levels in the area. Although ambient levels of heavy metals had no specific seasonal pattern, PAH levels showed distinct seasonal variations, with the highest level in winter. Factors affecting the PAH concentrations were vehicle exhaust, domestic heating, industrial activities, incineration within the area, and fuel combustion outside the area. The Sihwa-Banwol industrial complexes can be regarded as a hot-spot of PAH pollution in the Seoul metropolitan area.

Degrading characteristics of hydrocarbon and biosurfactant production by crude oil-degrading microorganisms

Kyung-Taek Ok(오경택),Myoung Jin Go(고명진),Gue Hwan Park(박귀환),Chang Min Kang(강창민),Seon Yong Chung(정선용) 한국생물공학회 2004 한국생물공학회 학술대회 Vol.2004 No.4

GPD 프로모터를 이용한 항균활성 효모의 활성증진

Min-Kyung Jang(장민경),Ki Hwan Yu(유기환),Nam-Young Kim(김남영),Ok-Hee Lee(이옥희),Jae-Kyun Shin(신재균),Hye Ji Jang(장혜지),Seungwoo Lee(이승우),Dong-Geun Lee(이동근),Sang-Hyeon Lee(이상현) 한국생명과학회 2010 생명과학회지 Vol.20 No.6

박테리오신의 항균활성 개선을 위해 선행연구에서 개발된 ADH 프로모터에 의해 박테리오신 유전자를 발현시키는 효모발현 벡터에 GPD 프로모터 단편을 도입하여 강력한 프로모터를 가진 효모발현 재조합 플라스미드 DNA를 제작하였다. ADH 프로모터에 의해 박테리오신 유전자를 발현시키는 기존의 형질전환 효모들에 비해 새롭게 개발된 GPD 프로모터에 의해 박테리오신 유전자를 발현시키는 형질전환 효모들이 그람양성 대표세균인 고초균(B. subtilis)과 그람음성 장내세균인 대장균(E. coli) 모두에서 보다 높은 생육억제환을 나타내는 것을 확인하였다. 이 연구의 결과로 GPD 프로모터에 의해 발현이 유도되는 박테리오신 생산 효모들을 이용하여 부패하기 쉬운 식품들의 보존성을 향상시키기 위한 보존제 대체물질 또는 가축 사료에서 병원균의 생육을 저해하기 위한 항생제 대체물질의 산업적인 생산이 가능하게 될 것으로 기대된다. We have previously reported recombinant productions of bacteriocins using yeast expression plasmid pAUR123, which contains the alcohol dehydrogenase (ADH) promoter, in Saccharomyces cerevisiae cells and their antibacterial activities. In order to improve the antibacterial activities of bacteriocidal yeast cells, a strong glyceraldehyde phosphate dehydrogenase (GPD) promoter gene of S. cerevisiae was amplified and inserted upstream into bacteriocin genes such as the OR-7, Subpeptin JM4-A or JM4-B gene in the corresponding recombinant yeast plasmid. Yeast cells transformed by the recombinant plasmid containing the GPD promoter represented higher antibacterial activities against both Gram positive B. subtilis and Gram negative E. coli cells compared to those transformed by the corresponding recombinant plasmid containing the ADH promoter. Thus, yeast cells harboring the recombinant plasmid containing the GPD promoter constructed in this study could be applied in the food preservative or animal feed industries.

Photosynthesis-Irradiance Relationship and Primary Production of Phytoplankton in Lake Gocheonam

( Min Kyung Jung ),( Ok Hee Lee ),( Kyung Je Cho ) 한국환경생물학회 2004 환경생물 : 환경생물학회지 Vol.22 No.4

N/A Photosynthetic activities and primary production of phytoplankton were investigated in Lake Gocheonam from October 1999 to August 2000. As an estuary lake with a barrage in the Southwestern coast of the Korean peninsula, the lake has received more attention after it became known as the habitat of large population of rare and endangered bird-Baikal Teal. As the lake had high algal biomass ranging from 20 μg chl-α L^(-1) to 125 μg chl-α L^(-1) in average values and rich eutrophication indicator species, the freshwaters were in a very productive or hypertrophic state. In the results obtained from the phytoplankton incubation in the laboratory, the maximum photosynthetic rate (P_(max)) varied according to seasons and sampling stations. Photo-synthetic activities were higher during the warm season than the cold seasons and the serial order of P_(max) was August dominated with Microcystis, April with Chlamydomonas and Nitzschia, October with Chlamydomonas and January with Stephanodiscus. The water of the lake was persistently turbid throughout the year due to strong winds from the adjacent sea. Despite the water turbidity, the phytoplankton productions estimated from a mathematical model had very broad range from 18 mgCm^(-2)day^(-1) to 10,300 mgCm^(-1)day^(-1).

Pandemic Novel Influenza A (H1N1) Virus in Korea: The Experience from August to September 2009

( Kyung Ok Lee ),( Min Young Park ),( Lyoung Hyo Kim ),( Hye Soon Seong ),( Bo Hyun Park ),( Su Jin Jeong ) 대한임상검사과학회 2009 대한임상검사과학회지(KJCLS) Vol.41 No.4

Novel influenza A virus, subtype H1N1 of swine-lineage, has been transmitted rapidly to many regions of the world. Rapid detection of the virus is essential to instigate appropriate patient care and public health management and for disease surveillance. The aim of this study is to determine the prevalence of novel influenza A (H1N1) virus in Korea using reverse-transcription real time polymerase chain reaction (rRT-PCR). Novel H1N1 virus was detected in a total of 8,948 nasopharyngeal samples from patients with influenza-like illness throughout Korea from August to September 2009. RNA was extracted from 300 μl of sample using an RNA extraction kit (Zymo Research, CA, USA). In the present study, Genekam kit (Genekam, Duisburg, Germany) was used to detect novel H1N1 virus. Novel H1N1 virus was found in 1,130 samples from a total of 8,948 samples (12.6%). The highest frequency was found in 10- to 19-year-olds (M: 29.3% vs. F: 16.4%), followed by 20- to 29-year-olds (M: 17.9% vs. F: 15.4%), 40- to 49-year-olds (M: 6.5% vs. F: 8.1%), 50- to 59-year-olds (M: 6.0% vs. F: 5.5%), and 30- to 39-year-olds (M: 4.6% vs. F: 3.8%). The mean positive rate was higher in men than in women (M: 14.7% vs. F: 7.4%). Novel H1N1 virus showed the lowest prevalence in patients over 60 years old. The positive rate increased daily and showed a significant high peak in mid-September 2009. In 19 provinces of Korea, Cheonan (41.1%), Busan (37.3%), Gangneung (33.3%), Jinju (32.1%), Ulsan (24.6%), Deajeon (23.7%) areas showed high frequencies and other provinces were found less than 10% of novel H1N1 virus. Since reverse-transcription real time PCR assay is rapid, accurate, and convenient, it may assist public health laboratories in detecting novel H1N1 virus. Moreover, these data could be useful for the management of patients with influenza-like illness.

Fermentation Patterns of Various Pectin Sources by Human Fecal Microbiota

Min, Byungjick,Kyung Koo, Ok,Park, Si Hong,Jarvis, Nathan,Ricke, Steven C.,Crandall, Philip G.,Lee, Sun-Ok 'Scientific Research Publishing, Inc.' 2015 Food and nutrition sciences Vol.6 No.12

Effects of Gastrocnemius and Lumbar Back Muscle Exercise on Standing Balance

Kyung Tae Yoo,Min Young An,Su Jung Eom,Bo Kyoung Kim,Joon Hee Lee,Jung Hyun Choi,Hee Joon Shin,Ok Kon Moon,Wan Suk Choi,Kyung Ok Min 국제물리치료학회 2013 Journal of International Academy of Physical Ther Vol.4 No.2

The purpose of this study was to test the effect of Gastrocnemius and Low Back-muscle isotonic exercise on static•dynamic standing balance during the period of 4 weeks. This study was two groups pretest-posttest design. Nineteen subjects who were over 22 years old were randomly assigned to either the experimental group that received the Gastrocnemius muscle exercise(n=9) or the low back muscle exercise(n=10) : The former group performed isotonic exercise(plantar flexion), the latter group performed isotonic exercise(trunk extension) a total of 18 times for three times per week for four weeks. Two groups also performed static and dynamic balance before the exercise and 4weeks after the exercise. The data were analyzed by using the paired t-test and independent t-test. The results were as follows: As compared with change of dynamic balance performance capacity at two groups, a significant difference was shown in the test(p<.05), but not in static balance(p>.05). Also, a significant difference of balance between groups was not shown in the test. In this study indicated that gastrocnemius and low back muscle isotonic exercise will have positive impact on standing balance.