Prognostic Value of TP53 Mutation for Transcatheter Arterial Chemoembolization Failure/Refractoriness in HBV-Related Advanced Hepatocellular Carcinoma

Miao Xue,Yanqin Wu,Wenzhe Fan,Jian Guo,Jialiang Wei,Hongyu Wang,Jizhou Tan,Yu Wang,Wang Yao,Yue Zhao,Jiaping Li 대한암학회 2020 Cancer Research and Treatment Vol.52 No.3

Purpose This study aimed to investigate the clinicopathologic features and mutational landscape of patients with hepatitis B virus (HBV)–related advanced hepatocellular carcinomas (HCC) undergoing transcatheter arterial chemoembolization (TACE). Materials and Methods From January 2017 to December 2018, 38 patients newly diagnosed with HBV-related advanced HCC were enrolled in the final analysis. Their pathological tissues and corresponding blood samples before TACE treatment were collected for whole-exome sequencing. Response to TACE was evaluated at 1-3 months after two consecutive use of TACE. Predictive factors were analyzed by univariate and multivariate analyses in a bivariate Logistic regression model. Enrichment of related pathways of all driver genes were acquired using the gene set enrichment analysis (GSEA). Results Among 38 patients, 23 (60.5%) exhibited TACE failure/refractoriness. Patients with TACE failure/refractoriness showed higher frequency of TP53 mutation than their counterparts (p=0.020). Univariate and multivariate analyses showed that only vascular invasion and TP53mutation were significantly correlated with TACE failure/refractoriness in HBV-related advanced HCC. Of the 16 patients without vascular invasion, eight (50.0%) had TP53 mutations, and TP53mutation was associated with TACE failure/refractoriness (p=0.041). Moreover, GSEA showed that mitogen-activated protein kinase and apoptosis pathways induced by TP53 mutation were possibly associated with TACE failure/refractoriness. Conclusion Our study suggested that TP53 mutation was independently related with TACE efficacy, which may work via mitogen-activated protein kinase and apoptosis pathways. These findings may provide evidence to help distinguish patients who will particularly benefit from TACE from those who require more personalized therapeutic regimens and rigorous surveillance in HBV-related advanced HCC.

Investigation on properties of autopolymerizing hydragel for denture soft lining material

Miao XUE,Jiao SUN,Wei HE 대한치과기재학회 2000 대한치과재료학회지 Vol.27 No.1

Denture soft lining materials have been recognized in prosthodontic clinical practice for many years. They act as enabling uniform distribution of pressure on denture-bearing tissues and reducing discomfort for patients with sharp or severely reabsorbed alveolar ridges and sensitive mucosa. However, the present two main kinds of soft liners still exist some shortcomings respectively, i.e. the plasticized acrylies may become harder and harder as the using time passed ; the silicone clastomers may present the weaker bond strength between the denture base resin and soft liner. Hydrophilic hydrogel is a polymer that can be expanded in water, remained the water molecular in its structure, but unresolved in water. There have been widely used in clinic such as plastic surgery, soft contact lens, ophthalmology, etc., also as a vehicle for the delivery of drugs. In this study, a new compound hydrogel was considered as a soft lining material. The reason for this assuming was that hydroxyl groups existed within its structure produced the hydrophilicity, while the backbone carbon chain ensured chemical and mechanical stability. The aim of this paper is to investigate the properties of a new prepared compound autopolymerizing hydrogel, including water sorption, solubility, bond strength and and biocompatibility.

Congratulatory Address

Miao XUE 대한치과재료학회 2000 대한치과기재학회 학술대회 Vol.2000 No.1

Insect-specific microRNA involved in the development of the silkworm Bombyx mori

Yong Zhang,Xue Zhou,Xie Ge,Jiang-Hao Jiang,Mu-Wang Li,Shi-Hai Jia,Xiao-Nan Yang,Yun-Chao Kan,Xue-Xia Miao,Guo-Ping Zhao,Fei Li,Yong-Ping Huang 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10

MicroRNAs (miRNAs) are endogenous non-coding genes that participate in post-transcription regulation by either degrading mRNA or blocking its translation. It is considered to be very important in regulating insect development and metamorphosis. Insects are the largest group of animals and are extremely valuable in biological and agriculture research. Insects are also important pests to human health and agriculture, and efforts are necessary protect both humans and plants from disease and damage. Despite their importance, insects lag behind mammals, nematodes, and plants in miRNA research. At present, only 279 insect miRNAs have been identified from Drosophila melanogaster, Anopheles gambiae, Apis mellifera, Bombyx mori, and D. pseudoobscura in miRBase, and most of these miRNAs were computationally predicted without experimental validation. Functional analysis of insect miRNAs has only been conducted in D. melanogaster.

Paying Attention to Students and Promoting Students` Mathematics Understanding

Miao Li,Jian Lan Tang,Xiao Xue Huang 한국수학교육학회 2008 수학교육연구 Vol.12 No.1

Dynamic Guaranteed Cost Compression for Time Series Big Data

Miao Bei-bei,Jin Xue-bo 보안공학연구지원센터 2015 International Journal of Database Theory and Appli Vol.8 No.4

Most time series big data is with noise and uncertain. To abstract the key information effectively and quickly, the estimation is one of the feasible methods for the uncertain big data. The Kalman filter with adaptive method by part of samples can give the high dimensional characteristics, reduce the computing cost and data uncertainty, but encounter the irregular estimation. The number of sample and the performance of the abstracted information have the tradeoff, which means we can use the suitable number of sample to abstract the key information of the series data. This paper discusses how to find the suitable sampling points for the time series data and the simulations show that the key dynamic information of time series big data can be guaranteed with the compression amount number of sample data.

Isolation and Characterization of Human Chorionic Membranes Mesenchymal Stem Cells and Their Neural Differentiation

Zongning Miao,선홍일,Yifeng Xue 한국조직공학과 재생의학회 2017 조직공학과 재생의학 Vol.14 No.2

Mesenchymal stem cells (MSCs) can be obtained from a variety of human tissues. Placenta has become an attractive stem cell source for potential applications in regenerative medicine and tissue engineering. The aim of this study was to localize and characterize MSCs within human chorionic membranes (hCMSCs). For this purpose, immunofluorescence labeling with CD105 and CD90 were used to determine the distribution of MSCs in chorionic membranes tissue. A medium supplemented with a synthetic serum and various concentrations of neurotrophic factors and cytokines was used to induce hCMSCs to neural cells. The results showed that the CD90 positive cells were scattered in the chorionic membranes tissue, and the CD105 positive cells were mostly located around the small blood vessels. hCMSCs expressed typical mesenchymal markers (CD73, CD90, CD105, CD44 and CD166) but not hematopoietic markers (CD45, CD34) and HLA-DR. hCMSCs differentiated into adipocytes, osteocytes, chondrocytes, and neuronal cells, as revealed by morphological changes, cell staining, immunofluorescence analyses, and RT-PCR showing the tissue-specific gene presence for differentiated cell lineages after the treatment with induce medium. Human chorionic membranes may be the source of MSCs for treatment of nervous system injury.

Large deviations for dependent heavy tailed random variables

Yu Miao,Tianyu Xue,Ke Wang,Fangfang Zhao 한국통계학회 2012 Journal of the Korean Statistical Society Vol.41 No.2

Let {Xn, n ≥ 1} be a stationary sequence of random variables with heavy tails. In this paper,we study the logarithmic asymptotic behaviors for the distributions of the partial sums Sn = ni =1 Xi under the assumption that {Xn, n ≥ 1} is a sequence of dependent random variables. Our main interest is in the crude estimates P(|Sn| > nx) ≈ n−αx+1 for appropriate values x where α is a specific parameter. Some results in this paper extend the works of Hu and Nyrhinen (2004).

Antioxidant effect of peony seed oil on aging mice

Xiao-Miao Han,Su-Xi Wu,Mei-Fang Wu,Xue-Feng Yang 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.6

D-galactose was injected into mice of ages 4–5 months, and peony seed oil was administered using an oral gavage to assess its possible anti-aging functions. The content of malondialdehyde (MDA) and the activities of monoamine oxidase (MAO), glutathione peroxidase (GSHPx), and superoxide dismutase (SOD) in the brain and liver of these mice were determined using biochemical kits. The significance of the differences in the content of the components associated with aging and anti-aging among each group was analyzed statistically. The MDA content and activities of MAO in the brain and liver of mice in the peony seed oil group were significantly lower than those in the aging group. The activities of GSH-Px, Cu/Zn-SOD, and Mn-SOD in the brain and liver of mice in the peony seed oil group were very significantly higher than those in the aging group. Peony seed oil was determined to have an obvious anti-aging function.

Simultaneous determination and difference evaluation of 14 ginsenosides in Panax ginseng roots cultivated in different areas and ages by high-performance liquid chromatography coupled with triple quadrupole mass spectrometer in the multiple reaction-monitoring mode combined with multivariate statistical analysis

Yang Xiu,Xue Li,Xiuli Sun,Dan Xiao,Rui Miao,Huanxi Zhao,Shuying Liu 고려인삼학회 2019 Journal of Ginseng Research Vol.43 No.4

Background: Ginsenosides are not only the principal bioactive components but also the important indexes to the quality assessment of Panax ginseng Meyer. Their contents in cultivated ginseng vary with the growth environment and age. The present study aimed at evaluating the significant difference between 36 cultivated ginseng of different cultivation areas and ages based on the simultaneously determined contents of 14 ginsenosides. Methods: A high-performance liquid chromatography (HPLC) coupled with triple quadrupole mass spectrometer (MS) method was developed and used in the multiple reaction-monitoring (MRM) mode (HPLC-MRM/MS) for the quantitative analysis of ginsenosides. Multivariate statistical analysis, such as principal component analysis and partial least squares-discriminant analysis, was applied to discriminate ginseng samples of various cultivation areas and ages and to discover the differentially accumulated ginsenoside markers. Results: The developed HPLC-MRM/MS method was validated to be precise, accurate, stable, sensitive, and repeatable for the simultaneous determination of 14 ginsenosides. It was found that the 3- and 5-yrold ginseng samples were differentiated distinctly by all means of multivariate statistical analysis, whereas the 4-yr-old samples exhibited similarity to either 3- or 5-yr-old samples in the contents of ginsenosides. Among the 14 detected ginsenosides, Rg1, Rb1, Rb2, Rc, 20(S)-Rf, 20(S)-Rh1, and Rb3 were identified as potential markers for the differentiation of cultivation ages. In addition, the 5-yr-old samples were able to be classified in cultivation area based on the contents of ginsenosides, whereas the 3- and 4-yr-old samples showed little differences in cultivation area. Conclusion: This study demonstrated that the HPLC-MRM/MS method combined with multivariate statistical analysis provides deep insight into the accumulation characteristics of ginsenosides and could be used to differentiate ginseng that are cultivated in different areas and ages.